Steady-state kinetic behaviour of two- or n-enzyme systems made of free sequential enzymes involved in a metabolic pathway.

Abstract:

:The overall rate of functioning of a set of free sequential enzymes of the Michaelis-Menten type involved in a metabolic pathway has been computed as a function of the concentration of the initial substrate under steady-state conditions. Curves monotonically increasing up to a saturation plateau have been obtained in all cases. The shape of these curves is sometimes, but not usually, close to that of a hyperbola. Cases exist in which the overall rate of reaction becomes quasi proportional to the concentration of initial substrate almost up to the saturation plateau, which never occurs with individual enzymes. Increasing the number of enzymes sequentially involved in a metabolic pathway does not seem to generate any particularly original behaviour compared with that of two-enzyme systems.

journal_name

C R Biol

journal_title

Comptes rendus biologies

authors

Legent G,Thellier M,Norris V,Ripoll C

doi

10.1016/j.crvi.2006.02.008

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

963-6

issue

12

eissn

1631-0691

issn

1768-3238

pii

S1631-0691(06)00197-1

journal_volume

329

pub_type

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