Detection of T.b. rhodesiense trypanosomes in humans and domestic animals in south east Uganda by amplification of serum resistance-associated gene.

Abstract:

:The human serum resistance-associated (SRA) gene was identified in 28 (80%) of the 35 T.b. rhodesiense trypanosomes from parasitologically confirmed sleeping sickness cases, using the primers designed by Radwanska and in 27 (77.1%) of the same 35 T.b. rhodesiense trypanosomes using the primers designed by Gibson. However, about 20% of the 35 T.b. rhodesiense trypanosomes could not be detected by SRA-polymerase chain reaction (PCR) even when an aliquot of the first PCR was used in the second PCR, indicating that the gene may be absent in those trypanosomes or the trypanosomes could be having another variant of SRA not detectable by these primers since three variants of SRA genes have so far been identified or the amount of trypanosomal DNA extracted from infected blood was too low to be detected. The trypanosome isolates that are SRA gene negative may indicate the presence of some T.b. rhodesiense trypanosomes with modified or lack SRA genes or simple loss of the SRA gene from the expression site in which it resides during antigenic variation. Analysis of trypanosomes derived from domestic animals showed that 79 (90.8%) of the 87 trypanosomes isolated from cattle were positive by Trypanosoma brucei (TBR)-PCR, indicating that they were Trypanozoon while 8 (9.2%) of the trypanosome isolates which were negative by TBR-PCR could be T. vivax, T. congolense, or T. theileri. When subjected to SRA-PCR, 10 (11.5%) of the 87 trypanosomes isolates derived from cattle were positive, indicating that there could be T.b. rhodesiense circulating in cattle, which is similar to the percentage of T.b. rhodesiense previously obtained in cattle in Serere, Soroti district.

journal_name

Ann N Y Acad Sci

authors

Enyaru JC,Matovu E,Nerima B,Akol M,Sebikali C

doi

10.1196/annals.1373.041

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

311-9

eissn

0077-8923

issn

1749-6632

pii

1081/1/311

journal_volume

1081

pub_type

杂志文章
  • Selective estrogen receptor modulator effects on serum lipoproteins and vascular function in postmenopausal women and in hypercholesterolemic men.

    abstract::Epidemiological observations, clinical mechanistic studies, and basic laboratory research suggest that estrogen therapy is associated with beneficial cardiovascular effects in postmenopausal women. Estrogen has a multitude of biological effects that may account for this apparent benefit (which remains to be proved in ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2001.tb04016.x

    authors: Blum A,Cannon RO 3rd

    更新日期:2001-12-01 00:00:00

  • Fish health--some concepts, constraints, and comparisons.

    abstract::This has been an overview of fish health, a comparison of some aspects of fish husbandry with other animal husbandry, and comments concerning fish health research--especially where it is going. The 1972 disease survey identified current problems; it ranked the problems according to priority. More important, research e...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1975.tb26826.x

    authors: Wolf K

    更新日期:1975-01-24 00:00:00

  • Hypothalamic inflammation: a double-edged sword to nutritional diseases.

    abstract::The hypothalamus is one of the master regulators of various physiological processes, including energy balance and nutrient metabolism. These regulatory functions are mediated by discrete hypothalamic regions that integrate metabolic sensing with neuroendocrine and neural controls of systemic physiology. Neurons and no...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2011.06388.x

    authors: Cai D,Liu T

    更新日期:2011-12-01 00:00:00

  • Application of risk assessment to international trade in animals and animal products.

    abstract::NAFTA and GATT have specified that we must base import decisions on sound scientific information. This includes the use of risk assessment procedures to justify excluding or restricting the international movement of either live animals or products from animals. Procedures and techniques have been described for calcula...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1996.tb53535.x

    authors: Metcalf HE,Blackwell JH,Acree JA

    更新日期:1996-07-23 00:00:00

  • Saliva testing for drugs of abuse.

    abstract::Saliva testing for drugs of abuse can provide both qualitative and quantitative information on the drug status of an individual undergoing testing. Self-administration by the oral, intranasal, and smoking routes often produces "shallow depots" of drug that contaminate the oral cavity. This depot produces elevated drug...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1993.tb18346.x

    authors: Cone EJ

    更新日期:1993-09-20 00:00:00

  • From organ to molecules: steps and consequences.

    abstract::The classic cardiac research programs revolved around measurable properties such as pressures, work done, vascular blood flow, electrical propagation, and other such parameters that defined the global heart functions in health and decease. Consistently, the first Henry Goldberg Workshop, held in Haifa in 1984, focused...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1341.001

    authors: Beyar R

    更新日期:2005-06-01 00:00:00

  • Genetic determinants of Paget's disease of bone.

    abstract::Paget's disease is a common skeletal disorder with a strong genetic component, which is characterized by focal increases in disorganized bone remodeling, predominantly affecting the axial skeleton. Current evidence suggests that classical Paget's disease of bone (PDB) is caused by a combination of rare alleles of larg...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2011.06228.x

    authors: Ralston SH,Albagha OM

    更新日期:2011-12-01 00:00:00

  • Peripheral markers of apoptosis in Parkinson's disease: the effect of dopaminergic drugs.

    abstract::In this study, we measured the lymphocyte levels of proteins involved in apoptosis regulation, such as Bcl-2, the peripheral benzodiazepine receptor (PBR), caspase-3, and Cu/Zn superoxide dismutase (Cu/Zn SOD), in patients with Parkinson's disease (PD), either untreated or under therapy with dopaminergic agents (l-Dop...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1299.123

    authors: Blandini F,Mangiagalli A,Cosentino M,Marino F,Samuele A,Rasini E,Fancellu R,Martignoni E,Riboldazzi G,Calandrella D,Frigo GM,Nappi G

    更新日期:2003-12-01 00:00:00

  • Longitudinal clinical course following pharmacological treatment of methamphetamine psychosis which persists after long-term abstinence.

    abstract::The present article investigated clinical symptoms and their longitudinal clinical course following pharmacological treatment in 32 female incarcerated patients suffering from methamphetamine (METH) psychosis who were referred to psychiatric consultation. The length of METH-abuse periods of the patients ranged from 2 ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1369.012

    authors: Akiyama K

    更新日期:2006-08-01 00:00:00

  • Criteria for selecting electrodes for electrical stimulation: theoretical and practical considerations.

    abstract::Smaller, more charge-intensive electrodes are needed for "safe" stimulation of the nervous system. In this paper we review critical concepts and the state of the art in electrodes. Control of charge density and charge balance are essential to avoid tissue electrolysis. Chemical criteria for "safe" stimulation are revi...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1983.tb31628.x

    authors: Brummer SB,Robblee LS,Hambrecht FT

    更新日期:1983-01-01 00:00:00

  • A method for characterization of total circulating DNA.

    abstract::Although much work has been done in the field of circulating DNA, no definitive information on sequencing data of total circulating DNA is available. Characterization of total circulating DNA by sequence analysis may give valuable information about the origin and function of these nucleic acid molecules. Circulating D...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1448.031

    authors: van der Vaart M,Pretorius PJ

    更新日期:2008-08-01 00:00:00

  • Aftereffects of vestibular and optokinetic stimulation and their interaction.

    abstract::In humans the influence of prior vestibular stimulation (3, 6, 9, 12, and 18 degrees/second2 for 10 seconds) and subsequent whole-field optokinetic stimulation (30, 60, 90, 120, and 180 degrees/second for 1 minute) or the presentation of a stationary pattern on after-nystagmus (AN) was studied. For comparison, pure ve...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1981.tb30889.x

    authors: Koenig E,Dichgans J

    更新日期:1981-01-01 00:00:00

  • Antigenic profile, prevalence, and clinical significance of antiphospholipid antibodies in women referred for in vitro fertilization.

    abstract::The aim of this prospective study was to assess the prevalence of antiphospholipid antibodies (aPL) in women who had undergone in vitro fertilization (IVF) and the relationship between aPL and IVF outcome. A total of 101 infertile women with at least three unsuccessful IVF attempts were consecutively included in this ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1422.048

    authors: Sanmarco M,Bardin N,Camoin L,Beziane A,Dignat-George F,Gamerre M,Porcu G

    更新日期:2007-06-01 00:00:00

  • The role of strategies in motor learning.

    abstract::There has been renewed interest in the role of strategies in sensorimotor learning. The combination of new behavioral methods and computational methods has begun to unravel the interaction between processes related to strategic control and processes related to motor adaptation. These processes may operate on very diff...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2011.06430.x

    authors: Taylor JA,Ivry RB

    更新日期:2012-03-01 00:00:00

  • Plasma levels of AGE peptides in type 1 diabetic patients are associated with serum creatinine and not with albumin excretion rate: possible role of AGE peptide-associated endothelial dysfunction.

    abstract::Patients with renal impairment have an increased risk for cardiovascular disease, which may be the result of advanced glycation end products (AGEs). The aim of this study was to investigate the levels of AGE peptides in relation to kidney function and to study the relationship of AGE peptides with endothelial function...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1333.075

    authors: Schalkwijk CG,Ter Wee PM,Stehouwer CD

    更新日期:2005-06-01 00:00:00

  • The role of input-output analysis of energy and ecologic systems. In the early development of ecological economics--a personal perspective.

    abstract::A summary is provided of the early history of research on the flow of nonrenewable energy resources through the economy and of the flow of renewable energy resources through a natural ecosystem. The techniques are similar, and many specific applications are provided. A combined economic and ecological technique is als...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2009.05165.x

    authors: Hannon B

    更新日期:2010-01-01 00:00:00

  • Immunogenicity of modified tumor cells in syngenic hosts.

    abstract::Modified tumor cells were used to immunize three murine hosts against syngenic ascitic lymphomas: C3H-6C3HED, BALB/c-P1798, and DBA/2-L1210. When the host was capable of a significant immune response against the malignant cells during progressive tumor growth (e.g., C3H vs. 6C3HED), protective immunization against a l...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1976.tb41636.x

    authors: Prager MD,Gordon WC,Baechtel FS

    更新日期:1976-01-01 00:00:00

  • Antisense promoter mapping. Inhibitory methods of transcriptional analysis.

    abstract::We have employed antisense methods to study the transcriptional functions of c-fos protein (Fos). Clones expressing inducible anti-fos RNA have been employed to inhibit c-fos expression, resulting in activation of c-fos transcription by inhibiting its normal repressor function. The sites of negative regulation by Fos ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1992.tb21061.x

    authors: Holt JT

    更新日期:1992-10-28 00:00:00

  • A regional approach to assess the impact of living in a chemical world.

    abstract::In the United States, some 80,000 commercial and industrial chemicals are now in use of which over 30,000 are produced or used in the Great Lakes region. Thus, the environmental quality within the Great Lakes basin has been compromised particularly with respect to persistent toxic substances (PTS). Information derived...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1371.028

    authors: De Rosa CT,Hicks HE,Ashizawa AE,Pohl HR,Mumtaz MM

    更新日期:2006-09-01 00:00:00

  • The integrative aspects of cardiac physiology and their implications for cell-based therapy.

    abstract::Cardiac development is characterized by a complex interplay of chemical, mechanical, and electrical forces, which together contribute to the proper formation of the heart muscle. In adult myocardium, cardiomyocytes are elongated, well-coupled by gap junctions, and organized in spatially well-defined muscle fibers. Thi...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2009.05077.x

    authors: Pijnappels DA,Gregoire S,Wu SM

    更新日期:2010-02-01 00:00:00

  • Is PR3-ANCA formation initiated in Wegener's granulomatosis lesions? Granulomas as potential lymphoid tissue maintaining autoantibody production.

    abstract::In Wegener's granulomatosis (WG), antiproteinase 3 (PR3) autoantibodies (PR3-ANCA) are crucial in the development of generalized vasculitis. Wegener's pathognomonic lesion, a granulomatous inflammation of the upper and lower respiratory tract, contains abundant lymphocytes and macrophages. Lymphocyte clusters in germi...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1361.042

    authors: Voswinkel J,Müller A,Lamprecht P

    更新日期:2005-06-01 00:00:00

  • Estrogens: neuroprotective or neurotoxic?

    abstract::The present paper reviews the major modes of action of estrogen on the molecular, cellular, tissue, and neurobehavioral levels of mammalian physiology, with an emphasis on the brain as an estrogen target tissue. We draw a distinction between receptor- and nonreceptor-mediated actions, as well as delineate the range of...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1999.tb07988.x

    authors: Scallet AC

    更新日期:1999-01-01 00:00:00

  • Purification of Cowdria ruminantium organisms for use in genome analysis by pulsed-field gel electrophoresis.

    abstract::Cowdria ruminantium is an obligate intracellular rickettsial pathogen which is responsible for a tick-borne disease of domestic and wild ruminants called heartwater or cowdriosis. Although several genes have been cloned and partially sequenced, the genome size, gross structure, and organization of the C. ruminantium g...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1998.tb11064.x

    authors: de Villiers EP,Brayton KA,Zweygarth E,Allsopp BA

    更新日期:1998-06-29 00:00:00

  • Role of nitric oxide in myocardial preconditioning.

    abstract::Ischemic preconditioning (PC) is a polygenic defensive cellular adaptive phenomenon whereby brief ischemic stimuli render the heart resistant to subsequent similar stress. The late phase of ischemic PC lasts for three to four days, protects against both myocardial stunning and infarction, and thus has considerable cli...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2002.tb04053.x

    authors: Dawn B,Bolli R

    更新日期:2002-05-01 00:00:00

  • Does the expression of c-kit (CD117) in neuroendocrine tumors represent a target for therapy?

    abstract::Neuroendocrine tumors are very heterogeneous, develop from a variety of tissues, and can be difficult to diagnose. Without the clinical manifestation of metastases, it is often difficult to characterize them as malignant. Even so-called completely (R0) resected tumors can spread clinically visible metastases within a ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1353.055

    authors: Koch CA,Gimm O,Vortmeyer AO,Al-Ali HK,Lamesch P,Ott R,Kluge R,Bierbach U,Tannapfel A

    更新日期:2006-08-01 00:00:00

  • Analysis of foreign protein overproduction in recombinant CHO cells. Effect of growth kinetics and cell cycle traverse.

    abstract::Intracellular foreign protein (beta-galactosidase) expression in recombinant CHO cell lines in continuous culture was analyzed by developing a mathematical model that includes the effects of metabolic burden and cell cycle dependence of intracellular foreign protein expression. This combined growth kinetic and cell cy...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1994.tb47392.x

    authors: Gu MB,Todd P,Kompala DS

    更新日期:1994-05-02 00:00:00

  • Synergistic effects of antiviral agents and humoral antibodies in experimental Herpesvirus hominis encephalitis.

    abstract::Experimental Herpesvirus hominis encephalitis in 3-week-old Swiss mice was utilized to evaluate the antiviral effects of adenine arabinoside (ara-A), phosphonacetic acid (PAA), and humoral antibodies to H. hominis (HIG). Administration of ara-A, PAA, or HIG during the early phase of infection reduced the mortality rat...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1977.tb21967.x

    authors: Cho CT,Feng KK

    更新日期:1977-03-04 00:00:00

  • Steroid biosynthesis within the frog brain: a model of neuroendocrine regulation.

    abstract::There is now clear evidence that the brain, similar to the adrenal gland, gonads, and placenta, is a steroidogenic organ. Notably in the frog brain, the presence of various steroidogenic enzymes has been detected by immunohistochemistry in specific populations of neurons and/or glial cells. These steroidogenic enzymes...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2008.03664.x

    authors: Do Rego JL,Seong JY,Burel D,Luu-The V,Larhammar D,Tsutsui K,Pelletier G,Tonon MC,Vaudry H

    更新日期:2009-04-01 00:00:00

  • Mechanisms of cerebrovascular amyloid deposition. Lessons from mouse models.

    abstract::Cerebrovascular deposition of amyloid is a frequent observation in Alzheimer's disease patients. It can also be detected sporadically in normal aged individuals and is further found in familial diseases linked to specific gene mutations. The source and mechanism of this pathology are still unknown. It has been suggest...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2000.tb06381.x

    authors: Burgermeister P,Calhoun ME,Winkler DT,Jucker M

    更新日期:2000-04-01 00:00:00

  • Rickettsial diseases in Russia.

    abstract::Currently, several rickettsioses are officially being reported in the Russian Federation. These are epidemic typhus and Brill-Zinsser disease, both caused by Rickettsia prowazekii which has a historic prevalence in Russia. Nowadays only single sporadic cases of R. prowazekii infection are reported. The last significan...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1374.006

    authors: Tarasevich IV,Mediannikov OY

    更新日期:2006-10-01 00:00:00