Smooth muscle alpha-actin expression and myofibroblast differentiation by TGFbeta are dependent upon MK2.


:Fibroblasts play a major role in processes such as wound repair, scarring, and fibrosis. Differentiation into myofibroblasts, characterized by upregulation of smooth muscle alpha-actin (smalpha) in response to profibrotic agents such as TGFbeta is believed to be an important step in fibrosis. Therefore, elucidating mechanisms of myofibroblast differentiation might reveal novel targets in treating diseases such as idiopathic pulmonary fibrosis (IPF). MK2 is a kinase substrate of p38 MAP kinase that mediates some effects of p38 activation on the actin cytoskeleton. Using mouse embryonic fibroblasts (MEF) from MK2 knockout (MK2(-/-)) mice, we demonstrate that disrupting expression of MK2 expression reduces filamentous actin and stress fibers. It also causes MK2(-/-) MEF to express less smalpha than their corresponding wild-type (WT) MEF at baseline and in response to TGFbeta. Furthermore, TGFbeta causes downregulation of smalpha in MK2(-/-) MEF, instead of upregulation observed in WT MEF. Expression of other fibroblast markers, such as collagen, is not altered in MK2(-/-) MEF. Our results further suggest that downregulation of smalpha in MK2(-/-) MEF is not due to lack of activation of serum responsive promoter elements, but probably due to reduced smalpha message stability in these cells. These results indicate that MK2 plays a key role in regulation of smalpha expression, and that targeting MK2 might present a therapeutic approach in managing conditions such as pulmonary fibrosis.


J Cell Biochem


Sousa AM,Liu T,Guevara O,Stevens J,Fanburg BL,Gaestel M,Toksoz D,Kayyali US




Has Abstract


2007-04-15 00:00:00












  • Constitutive and inducible expression of cytochromes P4501A (CYP1A1 and CYP1A2) in normal prostate and prostate cancer cells.

    abstract::Constitutive and benzo[a]pyrene (B[a]P) inducible expression of CYP1A1 and CYP1A2 in prostate cancer and normal prostate epithelial cells were examined by immunoblotting. Androgen independent prostate cancer cell lines DU145 and PC3 have constitutive expression of CYP1A and CYP1A1 and CYP1A2, respectively. Four microm...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sterling KM Jr,Cutroneo KR

    更新日期:2004-02-01 00:00:00

  • Sea urchin zygote chromatin exhibit an unfolded nucleosomal array during the first S phase.

    abstract::To investigate changes in chromatin organization associated with DNA replication during the first stages of development of the sea urchin Tetrapygus niger, we compared micrococcal nuclease (MNase) digestion patterns of chromatin from zygotes harvested during the first S phase and from unfertilized eggs. We observed th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Imschenetzky M,Puchi M,Gutierrez S,Montecino M

    更新日期:1995-10-01 00:00:00

  • Three-dimensional porous scaffolds at the crossroads of tissue engineering and cell-based gene therapy.

    abstract::In the last 20 years, more than 1,500 gene therapy clinical trials have been approved worldwide targeting a variety of indications, from inherited monogenic diseases to acquired conditions such as cancer, cardiovascular and infectious diseases. However, concerns about the safety and efficacy of gene therapy pharmaceut...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Coutu DL,Yousefi AM,Galipeau J

    更新日期:2009-10-15 00:00:00

  • Membrane dynamics of γ-secretase with the anterior pharynx-defective 1B subunit.

    abstract::The four-subunit protease complex γ-secretase cleaves many single-pass transmembrane (TM) substrates, including Notch and β-amyloid precursor protein to generate amyloid-β (Aβ), central to Alzheimer's disease. Two of the subunits anterior pharynx-defective 1 (APH-1) and presenilin (PS) exist in two homologous forms AP...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Dehury B,Kepp KP

    更新日期:2021-01-01 00:00:00

  • Vanadium induces apoptosis and modulates the expressions of metallothionein, Ki-67 nuclear antigen, and p53 during 2-acetylaminofluorene-induced rat liver preneoplasia.

    abstract::Our previous studies have shown that vanadium, a dietary micronutrient, has an inhibitory effect against experimentally induced rat hepatocarcinogenesis. In this study, we evaluated the role of vanadium on some potential protein expression markers of carcinogenesis, such as metallothionein (MT), an intracellular metal...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Chakraborty T,Samanta S,Ghosh B,Thirumoorthy N,Chatterjee M

    更新日期:2005-03-01 00:00:00

  • Global chimeric exchanges within the intracellular face of the bradykinin B2 receptor with corresponding angiotensin II type Ia receptor regions: generation of fully functional hybrids showing characteristic signaling of the AT1a receptor.

    abstract::The intracellular (IC) face of the G-protein coupled receptors (GPCR), bradykinin (BK) B2 and angiotensin (AT) 1a, is similar in sequence homology and in size. Both receptors are known to link to Galphai and Galphaq but differ markedly in a number of physiologic actions, particularly with respect to their hemodynamic ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yu J,Prado GN,Taylor L,Piserchio A,Gupta A,Mierke DF,Polgar P

    更新日期:2002-01-01 00:00:00

  • Differentiation in Acanthamoeba castellanii is induced by specific monoclonal antibodies.

    abstract::Monoclonal antibodies that bind a large molecular weight plasma membrane protein of Acanthamoeba castellanii cause the cells to differentiate. A different monoclonal antibody that binds specifically to the major plasma membrane protein has no effect upon cell division or differentiation. The induction of differentiati...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Villemez CL,Carlo PL,Russell MA

    更新日期:1985-01-01 00:00:00

  • Communication between the cell membrane and the nucleus: Role of protein compartmentalization.

    abstract::Understanding how the information is conveyed from outside to inside the cell is a critical challenge for all biologists involved in signal transduction. The flow of information initiated by cell-cell and cell-extracellular matrix contacts is mediated by the formation of adhesion complexes involving multiple proteins....

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Lelièvre SA,Bissell MJ

    更新日期:1998-01-01 00:00:00

  • Long noncoding RNA MEG3 inhibits breast cancer growth via upregulating endoplasmic reticulum stress and activating NF-κB and p53.

    abstract::Long noncoding RNA (lncRNA) maternally expressed 3 (MEG3) has been implicated as a tumor suppressor gene in several human cancer types. However, little is known regarding its involvement and potential mechanism in human breast cancer. In this study, we explored the effect of MEG3 on the growth of human breast cancer c...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zhang Y,Wu J,Jing H,Huang G,Sun Z,Xu S

    更新日期:2019-04-01 00:00:00

  • Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved Rotating Wall Vessel bioreactor (RWV).

    abstract::Weightlessness induces bone loss in humans and animal models. We employed the NASA-approved Rotating Wall Vessel bioreactor (RWV) to develop osteoblast-like cell cultures under microgravity and evaluate osteoblast phenotype and cell function. Rat osteoblast-like cell line (ROS.SMER#14) was grown in the RWV at a calcul...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Rucci N,Migliaccio S,Zani BM,Taranta A,Teti A

    更新日期:2002-01-01 00:00:00

  • Cigarette smoke extract regulates cytosolic phospholipase A2 expression via NADPH oxidase/MAPKs/AP-1 and p300 in human tracheal smooth muscle cells.

    abstract::Up-regulation of cytosolic phospholipase A(2) (cPLA(2)) by cigarette smoke extract (CSE) may play a critical role in airway inflammatory diseases. However, the mechanisms underlying CSE-induced cPLA(2) expression in human tracheal smooth muscle cells (HTSMCs) were not completely understood. Here, we demonstrated that ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Cheng SE,Lin CC,Lee IT,Hsu CK,Kou YR,Yang CM

    更新日期:2011-02-01 00:00:00

  • Calcium-protein interactions in the extracellular environment: calcium binding, activation, and immunolocalization of a collagenase/gelatinase activity expressed in the sea urchin embryo.

    abstract::We have purified and characterized a collagenase/gelatinase activity expressed during sea urchin embryonic development. The native molecular mass was determined to be 160 kDa, while gelatin substrate gel zymography revealed an active species of 41 kDa, suggesting that the native enzyme is a tetramer of active subunits...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Mayne J,Robinson JJ

    更新日期:1998-12-15 00:00:00

  • Role of microRNA-410 in molecular oncology: A double edged sword.

    abstract::MicroRNAs (miRNAs) are short non-coding single-stranded RNAs, which play significant roles in the regulation of a myriad of biological processes. Overwhelmingly increasing high-impact research has also deepened our understanding about the central role of miRNAs in cancer development, metastatic spread, and development...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Wen R,Umeano AC,Essegian DJ,Sabitaliyevich UY,Wang K,Farooqi AA

    更新日期:2018-11-01 00:00:00

  • Twist-ing cell fate: mechanistic insights into the role of twist in lineage specification/differentiation and tumorigenesis.

    abstract::Bone marrow-derived mesenchymal stem cells (MSC), are multipotent cells that give rise to multiple lineages including osteoblasts, adipocytes, muscle, and fibroblasts. MSCs are useful for clinical applications such as cell therapy because they can be isolated from an individual and expanded for use in tissue repair, a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Cakouros D,Raices RM,Gronthos S,Glackin CA

    更新日期:2010-08-15 00:00:00

  • PTEN Regulates Renal Extracellular Matrix Deposit via Increased CTGF in Diabetes Mellitus.

    abstract::Extracellular matrix accumulation and fibrosis are the features of diabetic nephropathy. PI3K (phosphatidylinositol 3-kinase)/Akt (protein kinase B) signal pathway and its inhibitor PTEN (phosphatase and tensin homolog deleted on chromosome 10) are revealed to modulate renal fibrosis. However, the exact mechanism is s...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zhu L,Zhao S,Liu S,Liu Q,Li F,Hao J

    更新日期:2016-05-01 00:00:00

  • Differentiation of HL-60 promyelocytic leukemia cells is accompanied by a modification of magnesium homeostasis.

    abstract::Magnesium homeostasis in HL-60 promyelocytic leukemia cells was compared to that in neutrophyl-like HL-60 cells obtained by 1.3% DMSO treatment. Magnesium homeostasis was studied by the characterization of magnesium efflux, the identification of intracellular magnesium pools, and the regulation of intracellular ionize...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wolf FI,Covacci V,Bruzzese N,Di Francesco A,Sacchetti A,Corda D,Cittadini A

    更新日期:1998-12-01 00:00:00

  • Molecular cloning of a tumor promoter-inducible mRNA found in JB6 mouse epidermal cells: induction is stable at high, but not at low, cell densities.

    abstract::From the mouse JB6 epidermal cell line C122 we have isolated a cDNA clone representing a 1.6-kilobase mRNA, called 2ar, that exhibits a biphasic induction in response to 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The first phase of induction in subconfluent cells is transient, peaking at 6 h after the addition of TP...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Smith JH,Denhardt DT

    更新日期:1987-05-01 00:00:00

  • Caspase inhibition and N6-benzyladenosine-induced apoptosis in HL-60 cells.

    abstract::As an extension of our recently published work (Mlejnek and Kuglík [2000] J. Cell. Biochem. 77:6-17), the role of caspases in N(6)-benzylaminopurine riboside (BAPR)-induced apotosis in HL-60 cells was evaluated in this study. Here, BAPR-induced apoptosis was accompanied by activation of caspase-3 and caspase-9. Howeve...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Mlejnek P

    更新日期:2001-01-01 00:00:00

  • Recent advances in understanding the mechanisms of osteoclast precursor fusion.

    abstract::Bone marrow macrophages fuse on the bone surface to form multinucleated osteoclasts that then organize to efficiently resorb bone. Many, if not all, of the stages of macrophage fusion involve cytoskeletal components that reorganize the cells. Recruitment may involve chemotactic responses to bone matrix protein and cal...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Oursler MJ

    更新日期:2010-08-01 00:00:00

  • Fibronectin promotes cervical cancer tumorigenesis through activating FAK signaling pathway.

    abstract::Cervical cancer is a cancer arising from the cervix, and it is the fourth most common cause of death in women. Overexpression of fibronectin 1 (FN1) was observed in many tumors and associated with the survival and metastasis of cancer cells. However, the mechanism by which FN1 promotes cervical cancer cell viability, ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zhou Y,Shu C,Huang Y

    更新日期:2019-04-11 00:00:00

  • Light and electron microscopic immunocytochemical localization of two major proteins in garlic bulb.

    abstract::Garlic is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, antidiabetic, and anticoagulant. Two major proteins of 40 KD and 14 KD constituting approximately 96% of total garlic proteins have been recently purified at our Institute. This immunocytochemic...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wen GY,Mato A,Wisniewski HM,Malik MN,Jenkins EC,Sheikh AM,Kim KS

    更新日期:1995-08-01 00:00:00

  • Matrix metalloproteinase (MMP)-12 regulates MMP-9 expression in interleukin-1beta-treated articular chondrocytes.

    abstract::Limited information is available on the expression and role of matrix metalloproteinase (MMP)-12 in chondrocytes. We characterized the expression mechanism of MMP-12 and possible function in chondrocytes. Interleukin (IL)-1beta induced the expression and activation of MMP-12 in primary culture chondrocytes and cartila...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Oh H,Yang S,Park M,Chun JS

    更新日期:2008-12-15 00:00:00

  • Comprehensive analysis of novel three-long noncoding RNA signatures as a diagnostic and prognostic biomarkers of human triple-negative breast cancer.

    abstract::Currently, traditional predictors of prognosis (tumor size, nodal status, progesterone receptor [PR], estrogen receptor [ER], or human epidermal growth factor receptor-2 [HER2]) are insufficient for precise survival prediction for triple-negative breast cancer (TNBC). Long noncoding RNAs (lncRNAs) have been observed t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Fan CN,Ma L,Liu N

    更新日期:2019-03-01 00:00:00

  • miR-144 reverses cisplatin resistance in cervical cancer via targeting LHX2.

    abstract::Mounting evidence showed that microRNAs involve in development and chemoresistance of various human cancers. We explored the roles and mechanisms of miR-144 in resistance to cisplatin (CDDP) of cervical cancer cells. miR-144 and LIM homeobox 2 (LHX2) expression in CDDP-resistant and the parental cells was determined b...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Shi F,Su J,Liu Z,Wang J,Wang T

    更新日期:2019-09-01 00:00:00

  • The metabolism of arachidonic and eicosapentaenoic acids in human neutrophils stimulated by A23187 and FMLP.

    abstract::A23187 stimulates the metabolism of endogenous as well as exogenous arachidonic acid (AA) and eicosapentaenolc acid (EPA) to their corresponding leukotrienes in human neutrophils. In contrast, conflicting results have been obtained concerning the effect of FMLP on the metabolism of these fatty acids. In the present st...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Mahadevappa VG,Powell WS

    更新日期:1989-07-01 00:00:00

  • Calmodulin binding to the Fas-mediated death-inducing signaling complex in cholangiocarcinoma cells.

    abstract::We have previously demonstrated that the antagonists of calmodulin (CaM) induce apoptosis of cholangiocarcinoma cells partially through Fas-mediated apoptosis pathways. Recently, CaM has been shown to bind to Fas, which is regulated during Fas or CaM antagonist-mediated apoptosis in Jurkat cells and osteoclasts. Accor...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Chen Y,Pawar P,Pan G,Ma L,Liu H,McDonald JM

    更新日期:2008-02-15 00:00:00

  • Embryonic stem cells and in vitro hematopoiesis.

    abstract::To study hematopoietic differentiation a variety of in vitro systems have been established using hematopoietic precursors derived from various explanted adult and fetal tissues. In this prospective we describe and discuss the potential of a novel system for studying the earliest stages of hematopoietic development. In...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Snodgrass HR,Schmitt RM,Bruyns E

    更新日期:1992-07-01 00:00:00

  • Association of vanadate-sensitive Mg(2+)-ATPase and shape change in intact red blood cells.

    abstract::Intact human erythrocytes, initially depleted of Mg2+ by EDTA incubation in the presence of A23187, exhibit Mg(2+)-dependent phosphate production of around 1.5 mmol per liter cells.h, half-maximally activated at around 0.4 mM added free Mg2+. This appears to correspond to Mg(2+)-stimulated adenosine triphosphatase (Mg...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Xu YH,Lu ZY,Conigrave AD,Auland ME,Roufogalis BD

    更新日期:1991-08-01 00:00:00

  • Decreased DMT1 and increased ferroportin 1 expression is the mechanisms of reduced iron retention in macrophages by erythropoietin in rats.

    abstract::Recycled iron from reticuloendothelial macrophages to erythroid precursors is important to maintain the iron homeostasis. However, the molecular mechanisms underlying iron homeostasis in macrophages are poorly understood. In this study, male Sprague-Dawley rats were treated with recombinant human erythropoietin (rHuEp...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Kong WN,Zhao SE,Duan XL,Yang Z,Qian ZM,Chang YZ

    更新日期:2008-05-15 00:00:00

  • Effect of homocysteinylation of low density lipoproteins on lipid peroxidation of human endothelial cells.

    abstract::Homocysteine-thiolactone (HcyT) is a toxic product whose synthesis is directly proportional to plasma homocysteine (Hcy) levels. Previous studies demonstrated that the interaction between HcyT and low density lipoproteins (LDL) induces the formation of homocystamide-LDL adducts (Hcy-LDL). Structural and functional alt...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Ferretti G,Bacchetti T,Moroni C,Vignini A,Nanetti L,Curatola G

    更新日期:2004-05-15 00:00:00