Abstract:
:The behavior of glutathione reductase (GR, EC 1.6.4.2) activity and isoforms were analyzed in wheat (Triticum aestivum L.) leaves and roots exposed to a chronic treatment with a toxic cadmium (Cd) concentration. A significant growth inhibition (up to 55%) was found in leaves at 7, 14 and 21 days, whereas roots were affected (51%) only after three weeks. Wheat plants grown in the presence of 100microM Cd showed a time-dependent accumulation of this metal, with Cd concentration being 10-fold higher in roots than in leaves. Nevertheless, lipid peroxidation was augmented in leaves in all experiments, but not in roots until 21 days. Cadmium treatment altered neither the GR activity nor the isoform pattern in the leaves. However, GR activity increased 111% and 200% in roots at 7 and 14 days, respectively, returning to control levels after 21 days. Three GR isoforms were found in roots of control and treated plants, two of which were enhanced by Cd treatment at 7 and 14 days, as assessed by activity staining on native gels. The changes in the isoform pattern modified the global kinetic properties of GR, thereby decreasing significantly (2.5-fold) the Michaelis constant (K(m)) value for oxidized glutathione. Isozyme induction was not associated with an enhancement of GR mRNA and protein expression, indicating that post-translational modification could occur. Our data demonstrated that up-regulation of GR activity by the induction of distinctive isoforms occurs as a defense mechanism against Cd-generated oxidative stress in roots.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Yannarelli GG,Fernández-Alvarez AJ,Santa-Cruz DM,Tomaro MLdoi
10.1016/j.phytochem.2006.11.016subject
Has Abstractpub_date
2007-02-01 00:00:00pages
505-12issue
4eissn
0031-9422issn
1873-3700pii
S0031-9422(06)00726-6journal_volume
68pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract::Four meroterpenoids, 1-hydroxychevalone C, 1-acetoxychevalone C, 1,11-dihydroxychevalone C, and 11-hydroxychevalone C and two ester epimers, 2S,4S-spinosate and 2S,4R-spinosate, together with seven known compounds, chevalones B, C, and E, tryptoquivaline, nortryptoquivaline, tryptoquivaline L, and quinadoline A were i...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2016.09.008
更新日期:2016-12-01 00:00:00
abstract::Three-day-old soybean (Glycine max) seedlings were exposed to 0.4 M sorbitol solution for 4 h to induce amidinotransferase activity, with the corresponding enzyme being purified to homogeneity by chromatographic separation on DEAE-Sephacel, Sephacryl S-300 and L-arginine Sepharose 4B. The purified enzyme used L-argini...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(02)00401-6
更新日期:2002-12-01 00:00:00
abstract::Three undescribed isoflavones, derriscandenon A, B, and C, together with seven known isoflavones were isolated and structurally characterized during a study of the chemical constituents in the leaves of Derris scandens (Roxb.) Benth (Leguminosae, Fabaceae) collected in Bangladesh. The inhibitory activity of the compou...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2020.112376
更新日期:2020-07-01 00:00:00
abstract::The dichloromethane extract from leaves of Juniperus brevifolia, through chromatographic fractionations yield six compounds: 3beta-hydroxy-abieta-8,11,13-trien-7-one, 18-hydroxy-sandaracopimara-8(14),15-dien-7-one, sandaracopimara-8(14),15-dien-18-yl formate; and the first examples of sandaracopimaranes and abieta-8,1...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2007.07.026
更新日期:2008-01-01 00:00:00
abstract::In a study of 62 plant species of the Cretan flora for their phytotoxic activity, plants were extracted successively with CH2Cl2, MeOH and H2O. Phytotoxicity evaluation of the 240 extracts was performed against Lactuca sativa L. and Agrostis stolonifera L.. The MeOH extract of Bellis longifolia was the most phytotoxic...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2017.08.019
更新日期:2017-12-01 00:00:00
abstract::Coumarins and meroterpene from the fruits of Cullen corylifolium were evaluated for their ability to bind and activate human estrogen receptor α (hERα) by a combination of in vitro studies and molecular dynamics simulations. The recombinant hERα ligand binding domain (hERα-LBD) was produced in BL21 (DE3)pLysS and the ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2018.05.010
更新日期:2018-08-01 00:00:00
abstract::Glycolipids of fresh latex from three clones of Hevea brasiliensis were characterized and quantified by HPLC/ESI-MS. Their fatty acyl and sterol components were further confirmed by GC/MS after saponification. The four detected glycolipid classes were steryl glucosides (SG), esterified steryl glucosides (ESG), monogal...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2011.04.023
更新日期:2011-10-01 00:00:00
abstract::Four cycloartane-type saponins, kahiricosides II-V (1-4), were isolated from the aerial parts of Astragalus kahiricus of Egyptian origin. Their structures were established as 9beta,19-cyclolanost-24E-ene-3beta,6alpha,16beta,27-tetraol-3-O-beta-D-glucopyranoside, 9beta,19-cyclolanost-24E-ene-3beta,6alpha,16beta,27-tetr...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2004.08.037
更新日期:2004-11-01 00:00:00
abstract::The phytohormones, auxin and ethylene, together control a wide range of physiological and developmental processes in plants. The lack of knowledge regarding how the underlying signaling processes are reflected at the protein level represents a major gap in understanding phytohormone signaling, including that mediated ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2012.08.007
更新日期:2012-12-01 00:00:00
abstract::Hyperforin is an important antidepressant constituent of Hypericum perforatum (St. John's wort). Cell cultures of the related species H. calycinum were found to contain the homologue adhyperforin and to a low extent hyperforin, when grown in BDS medium in the dark. Adhyperforin formation paralleled cell culture growth...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2004.11.003
更新日期:2005-01-01 00:00:00
abstract::In the course of our screening for new bioactive natural products, a culture of Hypoxylon rickii, a xylariaceous ascomycete collected from the Caribbean island Martinique, was identified as extraordinary prolific producer of secondary metabolites. Ten metabolites of terpenoid origin were isolated from submerged cultur...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2015.06.002
更新日期:2015-09-01 00:00:00
abstract::The incubation of 15α-hydroxy-ent-kaur-9(11),16-dien-19-oic acid (15α-hydroxy-grandiflorenic acid) with the fungus Fusarium fujikuroi gave as main metabolite its 3β,6β-dihydroxy derivative, which by an oxidative decarboxylation afforded a 19-nor compound with a 4,18-double bond. Other substances obtained were a 3α-hyd...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2013.01.006
更新日期:2013-05-01 00:00:00
abstract::Eight ring C-seco and ring-intact limonoids, and 25 known limonoids were isolated from the fruits of Melia toosendan (Meliaceae). Their structures were elucidated on the basis of extensive spectroscopic evidence including HRMS, 1D and 2D NMR spectroscopic data. A total of 29 isolated limonoids was evaluated for NF-κB ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2014.08.009
更新日期:2014-11-01 00:00:00
abstract::The Arabidopsis thaliana irregular xylem4 (irx4) cinnamoyl-CoA reductase 1 (CCR1) mutant was reassessed for its purported exclusive rate-limiting or key effects on lignification. Analyses of gross growth characteristics and stem cross-section anatomy, from seedling emergence to senescence, revealed that stunted irx4 m...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2004.12.016
更新日期:2005-09-01 00:00:00
abstract::Plant growth and defence are both fuelled by compounds synthesized from a common pool of carbon and nitrogen, implying the existence of a competition for carbon and nitrogen allocation to both metabolisms. The ratio of carbon to nitrogen (C:N) of an organ is often regarded as a convenient indicator of growth and quali...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2012.12.003
更新日期:2013-04-01 00:00:00
abstract::The ginseng pathogen Pythium irregulare is able to selectively metabolize the 20(S) protopanaxadiol ginsenosides Rb1, Rb2, Rc, Rd, and gypenoside XVII via extracellular glycosidases, leading to the formation and partial assimilation of ginsenoside F2. Herein we have partially purified three ginsenoside-deglycosylating...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2009.09.007
更新日期:2009-12-01 00:00:00
abstract::Phenylpropanoid glycosides, 1'-O-benzyl-alpha-L-rhamnopyranosyl-(1''-->6')-beta-D-glucopyranoside (1) and alpha-L-xylopyranosyl-(4''-->2')-(3-O-beta-D-glucopyranosyl)-1'-O-E-caffeoyl-beta-D-glucopyranoside (2), together with the known derivatives, 1,6-di-O-caffeoyl-beta-D-glucopyranoside (3), 1-O-(E)-caffeoyl-beta-D-g...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2005.06.019
更新日期:2005-08-01 00:00:00
abstract::Non-targeted LC-MS metabolomics on fruit of three wild and domesticated apple species (Malus sylvestris, M. sieversii and M. domestica) showed that two crab apple (M. sylvestris) accessions were distinguished by high concentrations of an ascorbic acid glycoside (AAG). This was partly purified, but key NMR signals were...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2020.112297
更新日期:2020-05-01 00:00:00
abstract::Green tissues of spikemoss Selaginella martensii Spring possessed the complex oxylipins patterns. Major oxylipins were the products of linoleic and α-linolenic acids metabolism via the sequential action of 13-lipoxygenase and divinyl ether synthase (DES) or allene oxide synthase (AOS). AOS products were represented by...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2015.08.003
更新日期:2015-10-01 00:00:00
abstract::Lotus japonicus roots, cultured in a modified B5 medium, produced and secreted germination stimulants that induced Striga hermonthica seed germination. The germination-inducing activity was detected both in the roots and the culture filtrate. Following bioassay-guided purification procedures, an active compound was is...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2007.06.011
更新日期:2008-01-01 00:00:00
abstract::Glucosylation is a predominant flavonoid modification reaction affecting the solubility, stability, and subsequent bioavailability of these metabolites. Flavonoid glycosides affect taste characteristics in citrus making the associated glucosyltransferases particularly interesting targets for biotechnology applications...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2009.07.027
更新日期:2009-07-01 00:00:00
abstract::Repeated fractionations of the methanol extract of the subterranean parts (rhizomes and roots) of Gentiana siphonantha afforded two new and five known secoiridoids, in addition to the widespread plant constituents beta-sitiosterol, daucosterol and oleanolic acid. The structures of the new acyl secoiridoid glycosides w...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(97)00225-2
更新日期:1997-11-01 00:00:00
abstract::Two isoflavanones (5,7-dihydroxy-2',3',4'-trimethoxy-isoflavanone and 5,7-dihydroxy-2'-methoxy-3',4'-methylenedioxy-isoflavanone), four isoflavanone O-glycosides (5,7-dihydroxy-2',3',4'-trimethoxy-isoflavanone 7-O-beta-glucopyranoside, 5,7-dihydroxy-2'-methoxy-3',4'-methylenedioxy-isoflavanone 7-O-beta-glucopyranoside...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2007.02.015
更新日期:2007-05-01 00:00:00
abstract::Both in vivo and in vitro ACC-oxidase activities as well as ethylene production from embryonic axes of chickpea seeds were strongly inhibited by EGTA, a selective extracellular Ca2+ ion chelator, indicating that the influx of Ca2+ is important for enzymatic activity. EGTA inhibition was restored by exogenous Ca2+. Tre...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(98)00591-3
更新日期:1999-02-01 00:00:00
abstract::Seven saponins named pithedulosides A-G were isolated from the seeds of Pithecellobium dulce. Their structures were established through spectral analyses as echinocystic acid 3-O-alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranoside, echinocystic acid and oleanolic acid 3-O-alpha-L-arabinopyranosyl- (1-->2)-alpha-L-...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(96)00725-x
更新日期:1997-04-01 00:00:00
abstract::Eleven triterpenoid saponins were isolated from the roots of Gypsophila trichotoma Wender. (G. trichotoma Wender. var. trichotoma) (Caryophyllaceae), together with one known compound. The structures were established on the basis of extensive NMR analysis ((1)H, (13)C NMR, COSY, TOCSY, ROESY, HSQC, and HMBC), completed...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2013.03.001
更新日期:2013-06-01 00:00:00
abstract::Three flavonoids and three saponins have been isolated from Oxytropis species. Their structures were determined as isorhamnetin-3-O-beta-D-glucoside, rhamnetin-3-O-beta-D-galactoside, apigenin, 3-O-[alpha-L-rhamnopyranosyl (1----2)-beta-D-glucopyranosyl(1----4)-beta-D-glucuronopyranosyl]+ ++soyasapogenol B, 3-O-[beta-...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/0031-9422(91)85128-m
更新日期:1991-01-01 00:00:00
abstract::Highly specialized chemical defense pathways are a particularly noteworthy metabolic characteristic of sessile organisms, whether terrestrial or marine, providing protection against pests and diseases. For this reason, knowledge of the metabolites involved in these processes is crucial to producing ecologically fit cr...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2014.07.024
更新日期:2015-05-01 00:00:00
abstract::From the branches of Microtropis japonica (Celastraceae), nine aliphatic glucosides, named microtropins A-I, were isolated. The 6-position of glucose was esterified with (2S,3R)-2-ethyl-2,3-dihydroxybutyric acid. Microtropins A-D contained a rare natured product nitrile functional group in their aglycones. The absolut...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2012.11.007
更新日期:2013-03-01 00:00:00
abstract::Three mature Eucalyptus globulus trees with 40 years of age were studied at three height levels (0%, 35%, and 60% of total tree height) and at three radial regions (sapwood, inner and outer heartwood). The composition of lipophilic extracts was evaluated and an extensive list of 202 compounds was reported for the firs...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2020.112520
更新日期:2020-12-01 00:00:00