The effect of storage in liquid nitrogen on the recovery of human dental plaque bacteria.

Abstract:

:Centrifuged deposits of various individual bacteria from type-culture collections and dental plaque samples were immersed in liquid nitrogen for periods of up to 2 years. All the organisms were recovered after storage in liquid nitrogen but Fusobacterium nucleatum (ATCC 10953) and Campylobacter fetus (NCTC 10354) consistently showed a reduction in the number of colony-forming units after freezing. The main effect on bacterial proportions in plaque samples was a reduction in aerobic Gram-negative cocci. Thus, storage in liquid nitrogen may be useful for comparative studies of dental plaque and as a means of storing individual organisms.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Wilson RF,Woods A,Ashley FP

doi

10.1016/0003-9969(84)90095-5

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

941-4

issue

11

eissn

0003-9969

issn

1879-1506

journal_volume

29

pub_type

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