Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering.

Abstract:

:Cardiac tissue engineering is focused on obtaining functional cardiomyocyte constructs to provide an alternative to cellular cardiomyoplasty. Mechanical stimuli have been shown to stimulate protein expression and the differentiation of mammalian cells from contractile tissues. Our aim was to obtain a flexible scaffold which could be used to apply mechanical forces during tissue regeneration. Poly(1,8-octanediol-co-citric acid) (POC) is an elastomer that can be processed into scaffolds for tissue engineering. We investigated the effect of modifying the porosity on the mechanical properties of the POC scaffolds. In addition, the effects of the storage method and strain rate on material integrity were assessed. The maximum elongation of POC porous films varied from 60% to 160% of their original length. A decrease in the porosity caused a rise in this elastic modulus. The attachment of HL-1 cardiomyocytes to POC was assessed on films coated with fibronectin, collagen and laminin. These extracellular matrix proteins promoted cell adhesion in a protein-type- and concentration-dependent manner. Therefore, POC scaffolds can be optimised to meet the mechanical and biological parameters needed for cardiac culture. This porous material has the potential to be used for cardiac tissue engineering as well as for other soft tissue applications.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

Hidalgo-Bastida LA,Barry JJ,Everitt NM,Rose FR,Buttery LD,Hall IP,Claycomb WC,Shakesheff KM

doi

10.1016/j.actbio.2006.12.006

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

457-62

issue

4

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(07)00004-9

journal_volume

3

pub_type

杂志文章
  • Substrate stiffness orchestrates epithelial cellular heterogeneity with controlled proliferative pattern via E-cadherin/β-catenin mechanotransduction.

    abstract:UNLABELLED:Epithelial cellular heterogeneity has been observed in pathological tissues with abnormal matrix stiffness and cells cultured on rigid substrates. However, it remains unclear how matrix stiffness influences cellular heterogeneity formation in multi-cellular population. Here, we demonstrated that cellular het...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.05.025

    authors: Wang B,Qin P,Zhao H,Xia T,Wang J,Liu L,Zhu L,Xu J,Huang C,Shi Y,Du Y

    更新日期:2016-09-01 00:00:00

  • Antiadhesion effect of the C17 glycerin ester of isoprenoid-type lipid forming a nonlamellar liquid crystal.

    abstract::Postoperative adhesion is a relevant clinical problem that causes a variety of clinical complications after abdominal surgery. The objective of this study is to develop a liquid-type antiadhesion agent and evaluate its efficacy in preventing tissue adhesion in a rat peritoneal adhesion model. The liquid-type agent was...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.12.009

    authors: Murakami T,Hijikuro I,Yamashita K,Tsunoda S,Hirai K,Suzuki T,Sakai Y,Tabata Y

    更新日期:2019-01-15 00:00:00

  • Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics.

    abstract::A predictive framework for the evolution of stem cell biology in 3-D is currently lacking. In this study we propose deep image informatics of the nuclear biology of stem cells to elucidate how 3-D biomaterials steer stem cell lineage phenotypes. The approach is based on high content imaging informatics to capture minu...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.08.052

    authors: Dhaliwal A,Brenner M,Wolujewicz P,Zhang Z,Mao Y,Batish M,Kohn J,Moghe PV

    更新日期:2016-11-01 00:00:00

  • Synthesis, mechanical and biological characterization of ionic doped carbonated hydroxyapatite/β-tricalcium phosphate mixtures.

    abstract::The influence of ionic substituents in calcium phosphates intended for bone and tooth replacement biomedical applications is an important research topic, owing to the essential roles played by trace elements in biological processes. The present study investigates the mechanical and biological evaluation of ionic doped...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.12.009

    authors: Kannan S,Vieira SI,Olhero SM,Torres PM,Pina S,da Cruz e Silva OA,Ferreira JM

    更新日期:2011-04-01 00:00:00

  • Peptide-mediated shape- and size-tunable synthesis of gold nanostructures.

    abstract::While several biological processes have been shown to be useful for the production of well-designed, inorganic nanostructures, the mechanism(s) controlling the size and shape of nano and micron size particles remains elusive. Here we report on the controlled size- and shape-specific production of gold nanostructures u...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.01.019

    authors: Kim J,Rheem Y,Yoo B,Chong Y,Bozhilov KN,Kim D,Sadowsky MJ,Hur HG,Myung NV

    更新日期:2010-07-01 00:00:00

  • Nanocomposite hydrogels stabilized by self-assembled multivalent bisphosphonate-magnesium nanoparticles mediate sustained release of magnesium ion and promote in-situ bone regeneration.

    abstract::Hydrogels are appealing biomaterials for applications in regenerative medicine due to their tunable physical and bioactive properties. Meanwhile, therapeutic metal ions, such as magnesium ion (Mg2+), not only regulate the cellular behaviors but also stimulate local bone formation and healing. However, the effective de...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.09.039

    authors: Zhang K,Lin S,Feng Q,Dong C,Yang Y,Li G,Bian L

    更新日期:2017-12-01 00:00:00

  • Screening of rat mesenchymal stem cell behaviour on polydimethylsiloxane stiffness gradients.

    abstract::Substrate stiffness is emerging as an effective tool for the regulation of cell behaviours such as locomotion, proliferation and differentiation. In order to explore the potential application of this biophysical tool, material platforms displaying lateral and continuously graded stiffness are advantageous since they a...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.09.030

    authors: Wang PY,Tsai WB,Voelcker NH

    更新日期:2012-02-01 00:00:00

  • A modular design strategy to integrate mechanotransduction concepts in scaffold-based bone tissue engineering.

    abstract::Repair or regeneration of load-bearing bones has long been an incentive for the tissue engineering community to develop a plethora of synthetic bone scaffolds. Despite the key role of physical forces and the mechanical environment in bone regeneration, the mechanotransduction concept has rarely been incorporated in st...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.10.012

    authors: Entezari A,Swain MV,Gooding JJ,Roohani I,Li Q

    更新日期:2020-12-01 00:00:00

  • Microelastic properties of lung cell-derived extracellular matrix.

    abstract::The mechanical properties of the extracellular microenvironment regulate cell behavior, including migration, proliferation and morphogenesis. Although the elastic moduli of synthetic materials have been studied, little is known about the properties of naturally produced extracellular matrix. Here we have utilized atom...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.07.021

    authors: Soucy PA,Werbin J,Heinz W,Hoh JH,Romer LH

    更新日期:2011-01-01 00:00:00

  • Self-assembled composite matrix in a hierarchical 3-D scaffold for bone tissue engineering.

    abstract::It is of high clinical relevance in bone tissue engineering that scaffolds promote a high seeding efficiency of cells capable of osteogenic differentiation, such as human bone marrow-derived mesenchymal stem cells (hMSCs). We evaluated the effects of a novel polycaprolactone (PCL) scaffold on hMSC seeding efficiency, ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.12.031

    authors: Chen M,Le DQ,Baatrup A,Nygaard JV,Hein S,Bjerre L,Kassem M,Zou X,Bünger C

    更新日期:2011-05-01 00:00:00

  • Synthesis of degradable-polar-hydrophobic-ionic co-polymeric microspheres by membrane emulsion photopolymerization: In vitro and in vivo studies.

    abstract::The synthesis of microspheres for tissue regeneration requires good control over the particle size and size distribution. This is particularly important when considering the immune response that may be triggered by the presence of particles in tissue. This report outlines the design of an injectable microsphere system...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.03.014

    authors: Tawagi E,Ganesh T,Cheng HM,Santerre JP

    更新日期:2019-04-15 00:00:00

  • Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance.

    abstract::Novel porous bilayered scaffolds, fully integrating a silk fibroin (SF) layer and a silk-nano calcium phosphate (silk-nanoCaP) layer for osteochondral defect (OCD) regeneration, were developed. Homogeneous porosity distribution was achieved in the scaffolds, with calcium phosphate phase only retained in the silk-nanoC...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.10.021

    authors: Yan LP,Silva-Correia J,Oliveira MB,Vilela C,Pereira H,Sousa RA,Mano JF,Oliveira AL,Oliveira JM,Reis RL

    更新日期:2015-01-01 00:00:00

  • Quantifying stent-induced damage in coronary arteries by investigating mechanical and structural alterations.

    abstract::Vascular damage develops with diverging severity during and after percutaneous coronary intervention with stent placement and is the prevailing stimulus for in-stent restenosis. Previous work has failed to link mechanical data obtained in a realistic in vivo or in vitro environment with data collected during imaging p...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.08.016

    authors: Geith MA,Nothdurfter L,Heiml M,Agrafiotis E,Gruber M,Sommer G,Schratzenstaller TG,Holzapfel GA

    更新日期:2020-10-15 00:00:00

  • Theoretical and experimental quantification of the role of diffusive chemogradients on neuritogenesis within three-dimensional collagen scaffolds.

    abstract::A critical challenge to regenerating close mimics of native axonal pathways under chronic neurodegenerative disease or injury conditions is the inability to stimulate, sustain and steer neurite outgrowth over a long distance, until they reach their intended targets. Understanding neurite outgrowth necessitates quantit...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.05.009

    authors: Kothapalli CR,Honarmandi P

    更新日期:2014-08-01 00:00:00

  • On the design of dental resin-based composites: a micromechanical approach.

    abstract::Adhesive resin-based restorative materials have the potential to considerably strengthen teeth and offer more economically viable alternatives to traditional materials such as gold, amalgam or ceramics. Other advantages are direct and immediate placement and the elimination of the use of mercury. However, polymerizati...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2007.06.011

    authors: Kahler B,Kotousov A,Swain MV

    更新日期:2008-01-01 00:00:00

  • Effect of subvoxel processes on non-destructive characterization of β-tricalcium phosphate bone graft substitutes.

    abstract::The geometric features of bone graft substitutes, such as the pore and pore interconnection sizes, are of paramount importance for their biological performance. Such features are generally characterized by micro-computed tomography (μCT). Unfortunately, the resolution of μCT is often too limited. The aim of this study...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.07.014

    authors: Bashoor-Zadeh M,Baroud G,Bohner M

    更新日期:2011-11-01 00:00:00

  • Bacterial microbots for acid-labile release of hybrid micelles to promote the synergistic antitumor efficacy.

    abstract::Bacteria have inherent properties of self-propelled navigation and specific infiltration into solid tumors. In the current study, we investigate a novel type of bacterial microbots for delivery of hybrid micelles to promote the synergistic antitumor efficacy. Escherichia coli Nissle 1917 (EcN) is used as a bacterial c...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.07.041

    authors: Xie S,Chen M,Song X,Zhang Z,Zhang Z,Chen Z,Li X

    更新日期:2018-09-15 00:00:00

  • High-density antimicrobial peptide coating with broad activity and low cytotoxicity against human cells.

    abstract:UNLABELLED:Medical device-associated infections are a multi-billion dollar burden for the worldwide healthcare systems. The modification of medical devices with non-leaching coatings capable of killing microorganisms on contact is one of the strategies being investigated to prevent microorganism colonization. Here we d...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.01.035

    authors: Rai A,Pinto S,Evangelista MB,Gil H,Kallip S,Ferreira MG,Ferreira L

    更新日期:2016-03-01 00:00:00

  • Inflammatory response against different carbon fiber-reinforced PEEK wear particles compared with UHMWPE in vivo.

    abstract::Poly(ether ether ketone) (PEEK) and its composites are recognized as alternative bearing materials for use in arthroplasty because of their mechanical properties. The objective of this project was to evaluate the biological response of two different kinds of carbon fiber-reinforced (CFR) PEEK compared with ultra-high ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.06.002

    authors: Utzschneider S,Becker F,Grupp TM,Sievers B,Paulus A,Gottschalk O,Jansson V

    更新日期:2010-11-01 00:00:00

  • A novel peptide for efficient siRNA delivery in vitro and therapeutics in vivo.

    abstract::Small interfering RNA (siRNA) shows great therapeutic potential due to its ability to regulate gene expression in a highly selective manner. However, its application has been limited by ineffective cellular uptake of siRNAs. To achieve successful gene-silencing efficiency, a safe and effective delivery vector is gener...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2015.04.002

    authors: Pan R,Xu W,Yuan F,Chu D,Ding Y,Chen B,Jafari M,Yuan Y,Chen P

    更新日期:2015-07-01 00:00:00

  • Material properties and osteogenic differentiation of marrow stromal cells on fiber-reinforced laminated hydrogel nanocomposites.

    abstract::The fibrils in the bone matrix are glued together by extracellular matrix proteins to form laminated structures (osteons) to provide elasticity and a supportive substrate for osteogenesis. The objective of this work was to investigate material properties and osteogenic differentiation of bone marrow stromal (BMS) cell...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.12.003

    authors: Xu W,Ma J,Jabbari E

    更新日期:2010-06-01 00:00:00

  • Hafnium-doped hydroxyapatite nanoparticles with ionizing radiation for lung cancer treatment.

    abstract:UNLABELLED:Recently, photodynamic therapy (PDT) is one of the new clinical options by generating cytotoxic reactive oxygen species (ROS) to kill cancer cells. However, the optical approach of PDT is limited by tissue penetration depth of visible light. In this study, we propose that a ROS-enhanced nanoparticle, hafnium...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.04.004

    authors: Chen MH,Hanagata N,Ikoma T,Huang JY,Li KY,Lin CP,Lin FH

    更新日期:2016-06-01 00:00:00

  • Influence of the amount of intermetallics on the degradation of Mg-Nd alloys under physiological conditions.

    abstract::The influence of amount of intermetallics on the degradation of as-extruded Mg-Nd alloys with different contents of Nd was investigated via immersion testing in DMEM+10% FBS under cell culture conditions and subsequent microstructural characterizations. It is found that the presence of intermetallic particles Mg41Nd5 ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.11.050

    authors: Zhang Y,Huang Y,Feyerabend F,Blawert C,Gan W,Maawad E,You S,Gavras S,Scharnagl N,Bode J,Vogt C,Zander D,Willumeit-Römer R,Kainer KU,Hort N

    更新日期:2021-02-01 00:00:00

  • Can human mesenchymal stem cells survive on a NiTi implant material subjected to cyclic loading?

    abstract::Nickel-titanium shape memory alloys (NiTi-SMAs) exhibit mechanical and chemical properties which make them attractive candidate materials for various types of biomedical applications. However, the high nickel content of NiTi-SMAs may result in adverse tissue reactions, especially when they are considered for load-bear...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.02.022

    authors: Habijan T,Glogowski T,Kühn S,Pohl M,Wittsiepe J,Greulich C,Eggeler G,Schildhauer TA,Köller M

    更新日期:2011-06-01 00:00:00

  • Species-specific effects of aortic valve decellularization.

    abstract::Decellularized heart valves have great potential as a stand-alone valve replacement or as a scaffold for tissue engineering heart valves. Before decellularized valves can be widely used clinically, regulatory standards require pre-clinical testing in an animal model, often sheep. Numerous decellularization protocols h...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.01.008

    authors: VeDepo MC,Buse EE,Quinn RW,Williams TD,Detamore MS,Hopkins RA,Converse GL

    更新日期:2017-03-01 00:00:00

  • Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model.

    abstract::Polymeric ultra-thin films (nanosheets) possess unique properties that make them suitable materials for various biomedical applications. In our previous study, we assessed the use of an antibiotic (tetracycline, TC)-loaded nanosheet (or "TC-nanosheet") for the treatment of gastrointestinal tissue defects. The nanoshee...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.04.019

    authors: Saito A,Miyazaki H,Fujie T,Ohtsubo S,Kinoshita M,Saitoh D,Takeoka S

    更新日期:2012-08-01 00:00:00

  • Photo-assisted generation of phospholipid polymer substrates for regiospecific protein conjugation and control of cell adhesion.

    abstract:UNLABELLED:Novel photo-reactive phospholipid polymers were synthesized for use in the preparation of nonfouling surfaces with protein conjugation capacity. Poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-ran-N-methacryloyl-(l)-tyrosinemethylester (MAT)] (P(MPC/MAT)) was synthesized by conventional radical polymeriz...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.03.023

    authors: Tanaka M,Iwasaki Y

    更新日期:2016-08-01 00:00:00

  • The chemical form of metallic debris in tissues surrounding metal-on-metal hips with unexplained failure.

    abstract::Implant-derived material from metal-on-metal (MOM) hip arthroplasties may be responsible for an unexplained tissue inflammatory response. The chemical form of the metal species in the tissues is predominantly chromium (Cr), but the currently used techniques have not been able to determine whether this is Cr(III) phosp...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.06.006

    authors: Hart AJ,Quinn PD,Sampson B,Sandison A,Atkinson KD,Skinner JA,Powell JJ,Mosselmans JF

    更新日期:2010-11-01 00:00:00

  • Impact of nanopore morphology on cell viability on mesoporous polymer and carbon surfaces.

    abstract::Topography at the nanoscale can lead to dramatic changes in the adhesion of cells to surfaces and their subsequent viability. For biological applications, including tissue engineering and cell-based sensing, the large internal surface area of ordered mesoporous carbons provides an opportunity for enhanced sensitivity ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.02.005

    authors: Chavez VL,Song L,Barua S,Li X,Wu Q,Zhao D,Rege K,Vogt BD

    更新日期:2010-08-01 00:00:00

  • Regulating the migration of smooth muscle cells by a vertically distributed poly(2-hydroxyethyl methacrylate) gradient on polymer brushes covalently immobilized with RGD peptides.

    abstract::The gradient localization of biological cues is of paramount importance to guide directional migration of cells. In this study, poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-block- poly(2-hydroxyethyl methacrylate) (P(HEMA-co-GMA)-b-PHEMA) brushes with a uniform underneath P(HEMA-co-GMA) layer and a gradi...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.05.046

    authors: Wu S,Du W,Duan Y,Zhang D,Liu Y,Wu B,Zou X,Ouyang H,Gao C

    更新日期:2018-07-15 00:00:00