当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > 4-Methylumbelliferyl-glycosides as fluorescence probes of sugar-binding sites on lectin molecules: spectral properties and dependence of fluorescence on polarity and viscosity.

4-Methylumbelliferyl-glycosides as fluorescence probes of sugar-binding sites on lectin molecules: spectral properties and dependence of fluorescence on polarity and viscosity.

Abstract：

:The spectral properties of 4-methylumbelliferyl-glycosides (MeUmb-glycosides) were investigated in order to assess their usefulness as probes of the microenvironment of sugar binding sites on lectin molecules. It was shown that the abnormally high values for fluorescence polarization of free MeUmb-glycosides (from 0.07 to 0.251) were due neither to their molecular size nor to the blockade of their movement, but to the short lifetimes (less than 0.55 ns) of the excited state of these compounds. Working essentially with two MeUmb-monosaccharides and one MeUmb-disaccharide (MeUmb-alpha-D-galactopyranoside, MeUmb-beta-D-galactopyranoside, and MeUmb-2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-beta-D- galactopyranoside) which were solubilized in various solvents, it was demonstrated that solvent polarity and viscosity definitely affected the fluorescence intensity of MeUmb-glycosides. A low-polarity medium reduced this intensity, and high viscosity enhanced it. The implications of these findings are discussed in relation to the variations in the fluorescence intensity of MeUmb-glycosides when these compounds were bound to lectins.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Decastel M,Vincent M,Matta KL,Frénoy JP

doi

10.1016/0003-9861(84)90584-8

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

640-53

issue

2

eissn

0003-9861

issn

1096-0384

pii

0003-9861(84)90584-8

journal_volume

232

pub_type

杂志文章

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