Hexose uptake in 7,12-dimethylbenz(a)anthracene-preexposed rat tracheal epithelial cells during the progression of neoplasia.

Abstract:

:Hexose uptake during the progression of neoplasia in rat tracheal epithelial cells was studied by measuring the uptake of 2-deoxy[3H]glucose (2-dGlc) in nontumorigenic (C-18) and tumorigenic (T-8, 1000-WT) rat tracheal epithelial cell lines with varying degrees of cell association as well as in: normal primary cell cultures (NPC) derived from explants of nonexposed tracheas; selected primary cell cultures (SPC) generated from explants of 7,12-dimethylbenz(a)anthracene-treated tracheal implants; and primary tumor cell cultures (TPC) derived from explants of 7,12-dimethylbenz(a)anthracene-induced tracheal carcinomas. The latter two groups represented cells from earlier and late stages in the progression of neoplasia, respectively, and each displayed an in vitro growth advantage that allowed for their survival and growth in medium devoid of supplements of pyruvate and insulin. This property was used in this study to select the carcinogen-altered cells from neighboring normal cells. Uptake of 2-dGlc per microgram of DNA was similar in subconfluent cultures of all cell lines. At confluency, uptake per microgram of DNA was reduced markedly (greater than 3-fold) in C-18 cells but it was reduced only 1.3-fold in T-8 cells and 1.6-fold in 1000-WT cells. Hexose uptake was further reduced in T-8 and 1000-WT cell cultures generated as outgrowths from explants of denuded tracheas bearing a reestablished epithelium from each cell line. Under these conditions, T-8 cells retained higher 2-dGlc uptake than did C-18, but uptake by 1000-WT was lower, indicating that tissue-like cell associations have a profound effect on hexose uptake in these epithelial cells. Results were generally similar when uptake was expressed per mg of protein although, in several instances, the interpretation of uptake data was affected by differences in the protein content between cultures (assessed by comparing protein:DNA ratios). Compared to NPC, hexose uptake was lower in SPC and one group of TPC. A second group of TPC, characterized by loose cell associations and much cell overlapping, had distinctly higher 2-dGlc uptake than did controls. Comparable results in these primary cultures were also observed when the number of cells per culture was used as a reference for 2-dGlc uptake. Under conditions of glucose deprivation, hexose uptake was increased in NPC and SPC. The production of lactic acid in each type of culture was dependent on the level of glucose in the medium, and this was nearly 2-fold greater in NPC than in SPC.(ABSTRACT TRUNCATED AT 400 WORDS)

journal_name

Cancer Res

journal_title

Cancer research

authors

Wasilenko WJ,Marchok AC

subject

Has Abstract

pub_date

1984-07-01 00:00:00

pages

3081-9

issue

7

eissn

0008-5472

issn

1538-7445

journal_volume

44

pub_type

杂志文章
  • Insulin-dependent leptin expression in breast cancer cells.

    abstract::Pathologic conditions associated with hyperinsulinemia, such as obesity, metabolic syndrome, and diabetes, seem to increase the risk of breast cancer. Here, we studied molecular mechanisms by which insulin activates the expression of leptin, an obesity hormone that has been shown to promote breast cancer progression i...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-0642

    authors: Bartella V,Cascio S,Fiorio E,Auriemma A,Russo A,Surmacz E

    更新日期:2008-06-15 00:00:00

  • Multiple hepatic receptors cooperate to eliminate secretory mucins aberrantly entering the bloodstream: are circulating cancer mucins the "tip of the iceberg"?

    abstract::Hollow organs lined by columnar epithelial cells normally secrete mucins and their proteolytic fragments vectorially into the lumen. These heterogeneously O-glycosylated molecules are known to aberrantly enter the bloodstream in the setting of epithelial carcinomas and possibly during injury or inflammation. We have r...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-3851

    authors: Wahrenbrock MG,Varki A

    更新日期:2006-02-15 00:00:00

  • Galectin-1 drives pancreatic carcinogenesis through stroma remodeling and Hedgehog signaling activation.

    abstract::Despite some advances, pancreatic ductal adenocarcinoma (PDAC) remains generally refractory to current treatments. Desmoplastic stroma, a consistent hallmark of PDAC, has emerged as a major source of therapeutic resistance and thus potentially promising targets for improved treatment. The glycan-binding protein galect...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-13-3013

    authors: Martínez-Bosch N,Fernández-Barrena MG,Moreno M,Ortiz-Zapater E,Munné-Collado J,Iglesias M,André S,Gabius HJ,Hwang RF,Poirier F,Navas C,Guerra C,Fernández-Zapico ME,Navarro P

    更新日期:2014-07-01 00:00:00

  • In vivo detection of gastric cancer in rats by electron paramagnetic resonance imaging.

    abstract::Electron paramagnetic resonance imaging (EPRI) enables noninvasive spatial mapping of free radical metabolism and has recently been shown to provide in vivo physiologic information regarding alterations in the redox state of tumors and neoplastic tissues. With the use of nitroxide spin probes, it has been shown that c...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-04-0319

    authors: Mikuni T,He G,Petryakov S,Fallouh MM,Deng Y,Ishihara R,Kuppusamy P,Tatsuta M,Zweier JL

    更新日期:2004-09-15 00:00:00

  • A cytokinetic analysis of bacillus calmette-guérin-induced growth control of a murine leukemia.

    abstract::The cytokinetics of an isogeneic, transplantable, lymphoid leukemia, growing as an ascitic tumor in the C57BL/6 mouse, has been investigated during normal growth and during regression induced by weekly injections i.v. of 1.0 mg Bacillus Calmette-Guérin (BCG). Survival was significantly prolonged in the BCG-treated gro...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Olsson L,Mathé G

    更新日期:1977-06-01 00:00:00

  • Chemoprevention of spontaneous intestinal adenomas in the Apc Min mouse model by the nonsteroidal anti-inflammatory drug piroxicam.

    abstract::C57BL/6J-Min/+mice (n = 56), heterozygous for a nonsense mutation in the Apc gene, were randomized at weaning to seven groups, including groups treated with piroxicam at 0, 50, 100, and 200 ppm in the AIN93G diet. After only 6 weeks of treatment, intestinal adenomas and aberrant crypt foci were counted, and serum leve...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Jacoby RF,Marshall DJ,Newton MA,Novakovic K,Tutsch K,Cole CE,Lubet RA,Kelloff GJ,Verma A,Moser AR,Dove WF

    更新日期:1996-02-15 00:00:00

  • Pathogenesis of ascites tumor growth: fibrinogen influx and fibrin accumulation in tissues lining the peritoneal cavity.

    abstract::In the immediately preceding paper, we demonstrated that the microvasculature supplying peritoneal lining tissues of mice bearing either of two transplantable ascites carcinomas was hyperpermeable to circulating macromolecules. Solid tumors have been shown to exhibit similar levels of microvascular hyperpermeability, ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Nagy JA,Meyers MS,Masse EM,Herzberg KT,Dvorak HF

    更新日期:1995-01-15 00:00:00

  • Metformin Inhibits Progression of Head and Neck Squamous Cell Carcinoma by Acting Directly on Carcinoma-Initiating Cells.

    abstract::Metformin may reduce the progression of head and neck squamous cell carcinoma (HNSCC); however, whether metformin acts by altering the host metabolism or targets cancer-initiating cells remains poorly understood. This gap in knowledge has prevented the stratification of patient populations who are most likely to benef...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-18-3525

    authors: Wu X,Yeerna H,Goto Y,Ando T,Wu VH,Zhang X,Wang Z,Amornphimoltham P,Murphy AN,Tamayo P,Chen Q,Lippman SM,Gutkind JS

    更新日期:2019-09-01 00:00:00

  • Quantitative measurements of the changes in protein thiols in cervical intraepithelial neoplasia and in carcinoma of the human uterine cervix provide evidence for the existence of a biochemical field effect.

    abstract::Quantitative micromethods have been used for measuring reactive protein thiols (PSHr), total reactive protein sulfur (TRPS), total protein thiols (PSHt), and protein disulfides (PDS) in fixed frozen sections of human uterine cervix. PSHr and TRPS were stained using 2,2'-dihydroxy-6,6'-dinaphthyl disulfide; PSHt and PD...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Benedetto C,Bajardi F,Ghiringhello B,Marozio L,Nöhammer G,Phitakpraiwan P,Rojanapo W,Schauenstein E,Slater TF

    更新日期:1990-10-15 00:00:00

  • Microcytotoxicity assay of immune responses to non-mammary tumor virus-induced, preneoplastic, and neoplastic mammary lesions in BALB/c mice.

    abstract::Using a microcytotoxicity assay we have studied the immune responses to the preneoplastic, hyperplastic alveolar nodule (HAN) lines D1 and D2 and to the mammary tumors arising spontaneously from them in BALB/c mice. Lymph node cells (LNC) from mice bearing HAN implants, or from mice whose implants had been removed gre...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Heppner GH,Kopp JS,Medina D

    更新日期:1976-02-01 00:00:00

  • Increased expression of transforming growth factor beta isoforms and basic fibroblast growth factor in complex hyperplasia and adenocarcinoma of the endometrium: evidence for paracrine and autocrine action.

    abstract::Endometrial carcinoma is associated with antecedent simple and complex hyperplasia, and the endometrium is a target tissue for the action of cytokines and growth factors. Transforming growth factor (TGF)-beta s are potent cellular growth and differentiation regulatory factors. Therefore, we investigated the potential ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Gold LI,Saxena B,Mittal KR,Marmor M,Goswami S,Nactigal L,Korc M,Demopoulos RI

    更新日期:1994-05-01 00:00:00

  • Functional CXCR4-expressing microparticles and SDF-1 correlate with circulating acute myelogenous leukemia cells.

    abstract::Stromal cell-derived factor-1 (SDF-1/CXCL12) and its receptor CXCR4 are implicated in the pathogenesis and prognosis of acute myelogenous leukemia (AML). Cellular microparticles, submicron vesicles shed from the plasma membrane of various cells, are also associated with human pathology. In the present study, we invest...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-2006

    authors: Kalinkovich A,Tavor S,Avigdor A,Kahn J,Brill A,Petit I,Goichberg P,Tesio M,Netzer N,Naparstek E,Hardan I,Nagler A,Resnick I,Tsimanis A,Lapidot T

    更新日期:2006-11-15 00:00:00

  • Doxorubicin plus interleukin-2 chemoimmunotherapy against breast cancer in mice.

    abstract::As recently characterized, following s.c. implantation into syngeneic C57BL/6 mice, E0771 tumor invades locally into dermal layers and peritoneum, metastasizes to the lung, and induces a nonspecific immunosuppression in the host. Using this breast medullar adenocarcinoma model, a therapy consisting of a single moderat...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-3963

    authors: Ewens A,Luo L,Berleth E,Alderfer J,Wollman R,Hafeez BB,Kanter P,Mihich E,Ehrke MJ

    更新日期:2006-05-15 00:00:00

  • Major increases in spermidine/spermine-N1-acetyltransferase activity by spermine analogues and their relationship to polyamine depletion and growth inhibition in L1210 cells.

    abstract::As an antiproliferative strategy, we are using bis(ethyl) derivatives of spermine to suppress polyamine biosynthetic enzyme activity and, thereby, deplete intracellular polyamine pools. Since certain of these analogues have recently been shown to potently increase spermidine/spermine-N1-acetyltransferase activity, we ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Libby PR,Henderson M,Bergeron RJ,Porter CW

    更新日期:1989-11-15 00:00:00

  • Effect of nerve growth factor on the transplacental induction of neurinomas by ethylnitrosourea in Sprague-Dawley rats.

    abstract::Administration of nerve growth factor (NGF) to the offspring of Sprague-Dawley rats transplacentally exposed to 50 mg/kg ethylnitrosourea on the 20th day of gestation resulted in a significant reduction of trigeminal and peripheral nerve neurinomas. Forty, 60, and 80 micrograms of NGF was administered in five s.c. dos...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Raju NR,Koestner A,Marushige K,Lovell KL,Okazaki D

    更新日期:1989-12-15 00:00:00

  • Distinct differentiation-inducing activities of gamma-interferon and cytokine factors acting on the human promyelocytic leukemia cell line HL-60.

    abstract::The human promyelocytic leukemia cell line HL-60 and monoblastic leukemia cell line U937 undergo differentiation when induced by lymphokine and cytokine preparations. Growth inhibition, acquisition of immunoglobulin Fc receptors, increased expression of monocyte-related surface antigens, and an increase in lysosomal e...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Harris PE,Ralph P,Gabrilove J,Welte K,Karmali R,Moore MA

    更新日期:1985-07-01 00:00:00

  • Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice.

    abstract::The inhibitor of apoptosis proteins (IAPs) are a family of antiapoptotic proteins that bind and inhibit caspases 3, 7, and/or 9, but not caspase 8. Growing evidence also indicates that IAPs also modulate cell division, cell cycle progression, and signal transduction pathways. As our basic understanding of IAPs has inc...

    journal_title:Cancer research

    pub_type: 杂志文章,评审

    doi:10.1158/0008-5472.CAN-04-1918

    authors: Schimmer AD

    更新日期:2004-10-15 00:00:00

  • Effects of metabolism on the binding of polycyclic hydrocarbons to nuclear subfractions of cultured AKR mouse embryo cells.

    abstract::A high-affinity localization of [2H]methylcholanthrene and/or its metabolites to a specific nuclear fraction (Fraction l) between 4 and 72 hr of exposure is described. Other carcinogenic hydrocarbons, such as benzo(a)pyrene and dibenz(a,h)anthracene, demonstrate a similar markded localization in Fraction l after 24 hr...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Spelsberg TC,Zytkovicz TH,Moses HL

    更新日期:1977-05-01 00:00:00

  • Schedule-dependent antitumor effects of methotrexate and 5-fluorouracil.

    abstract::The antitumor effects of methotrexate with 5-fluorouracil against the Sarcoma 180 mouse tumor model were found to be schedule dependent. Pretreatment of tumor-bearing animals with methotrexate significantly enhanced the antitumor activity of the combination relative to simultaneous treatment or to those with methotrex...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Bertino JR,Sawicki WL,Lindquist CA,Gupta VS

    更新日期:1977-01-01 00:00:00

  • Dominant-negative mutations of the tumor suppressor p53 relating to early onset of glioblastoma multiforme.

    abstract::Previous experiments have suggested that some mutant forms of p53 are able to inactivate the endogenous wild-type p53 protein in a dominant-negative fashion. However, it remains unknown whether tumors with such dominant-negative (transdominant) p53 mutants have a biological significance that is different from that of ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Marutani M,Tonoki H,Tada M,Takahashi M,Kashiwazaki H,Hida Y,Hamada J,Asaka M,Moriuchi T

    更新日期:1999-10-01 00:00:00

  • Monoclonal antibodies directed against preneoplastic and neoplastic murine mammary lesions.

    abstract::We have produced a panel of monoclonal antibodies directed against a dimethylbenzanthracene-induced murine mammary tumor. Five rat-mouse hybridomas produced antibodies that bound to some murine mammary tumors, but not to normal renal adherent cells, lymphocytes, 3T3 fibroblasts, red blood cells, or mammary gland. One ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Johnson CW,Wei WZ,Barth RF,Tuttle SE,Andrews CA

    更新日期:1985-08-01 00:00:00

  • DNA in situ sensitivity to denaturation as a marker of human breast tumors.

    abstract::DNA content and in situ sensitivity to denaturation were analyzed by flow cytometry of individual cell nuclei isolated from 40 breast carcinomas, nine fibroadenomas, and 14 samples of normal breast tissue. The extent of DNA denaturation induced by acid was expressed as alpha t, which represents the fraction of DNA sta...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kunicka JE,Olszewski W,Rosen PP,Kimmel M,Melamed MR,Darzynkiewicz Z

    更新日期:1989-11-15 00:00:00

  • ATM acts downstream of ATR in the DNA damage response signaling of bystander cells.

    abstract::This study identifies ataxia-telangiectasia mutated (ATM) as a further component of the complex signaling network of radiation-induced DNA damage in nontargeted bystander cells downstream of ataxia-telangiectasia and Rad3-related (ATR) and provides a rationale for molecular targeted modulation of these effects. In dir...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-0545

    authors: Burdak-Rothkamm S,Rothkamm K,Prise KM

    更新日期:2008-09-01 00:00:00

  • Assessment of protein kinase C isozymes by enzyme immunoassay and overexpression of type II in thyroid adenocarcinoma.

    abstract::A two site enzyme immunoassay which quantitatively identifies types I, II, and III of protein kinase C isozymes has been designed. The soluble protein kinase C isozymes were selectively immobilized by type-specific monoclonal antibodies, MC-1a, -2a, and -3a (H. Hidaka et al., J Biol. Chem., 263: 4523-4526, 1988) which...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Hagiwara M,Hachiya T,Watanabe M,Usuda N,Iida F,Tamai K,Hidaka H

    更新日期:1990-09-01 00:00:00

  • Cooperation between v-fos and v-rasHA induces autonomous papillomas in transgenic epidermis but not malignant conversion.

    abstract::Transgenic mice have been previously established that express v-rasHa or v-fos exclusively in the epidermis by means of a targeting vector based on the human keratin 1 gene (HK1). Epidermal expression of v-rasHa (HK1.ras) or v-fos (HK1.fos) resulted in hyperplasia, hyperkeratosis, and later, in benign tumors. To asses...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Greenhalgh DA,Quintanilla MI,Orengo CC,Barber JL,Eckhardt JN,Rothnagel JA,Roop DR

    更新日期:1993-11-01 00:00:00

  • Phorbol ester-induced differentiation of a non-T/non-B human leukemic cell line (REH) to macrophage-like cells.

    abstract::Treatment of non-T/non-B human leukemic cell line REH with 5 X 10(-9) M 12-O-tetradecanoylphorbol-13-acetate (TPA) at 37 degrees resulted in their adherence to culture flasks by 24 to 36 hr and, after 72 hr, the entire surface of the flask/coverslips became covered with macrophage-like cells containing pseudopodia. Wr...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Srivastava BI,Koga M,Srivastava P

    更新日期:1984-07-01 00:00:00

  • The suppression of drug-induced apoptosis by activation of v-ABL protein tyrosine kinase.

    abstract::Cells with a temperature-sensitive mutant of the v-abl oncoprotein (IC.DP) were treated with the anticancer drugs melphalan or hydroxyurea. At the restrictive temperature for v-ABL protein tyrosine kinase activity, drug-treated IC.DP cells died by apoptosis. In contrast, apoptotic cell death induced by either drug was...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Chapman RS,Whetton AD,Dive C

    更新日期:1994-10-01 00:00:00

  • BRG1/SMARCA4 inactivation promotes non-small cell lung cancer aggressiveness by altering chromatin organization.

    abstract::SWI/SNF chromatin remodeling complexes regulate critical cellular processes, including cell-cycle control, programmed cell death, differentiation, genomic instability, and DNA repair. Inactivation of this class of chromatin remodeling complex has been associated with a variety of malignancies, including lung, ovarian,...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-14-0061

    authors: Orvis T,Hepperla A,Walter V,Song S,Simon J,Parker J,Wilkerson MD,Desai N,Major MB,Hayes DN,Davis IJ,Weissman B

    更新日期:2014-11-15 00:00:00

  • Immunohistochemical analysis of human pulmonary carcinomas using monoclonal antibody 44-3A6.

    abstract::A monoclonal antibody, 44-3A6, was raised against the human pulmonary adenocarcinoma cell line A549. This antibody recognizes a protein antigen at the cell surface, which is preserved after formalin fixation and paraffin embedding. Immunohistochemical staining of lung tissue with this antibody revealed diffuse immunor...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Lee I,Radosevich JA,Ma YX,Combs SG,Rosen ST,Gould VE

    更新日期:1985-11-01 00:00:00

  • Selective protection against cis-diamminedichloroplatinum(II)-induced toxicity in kidney, gut, and bone marrow by diethyldithiocarbamate.

    abstract::Diethyldithiocarbamate (DDTC) has been shown to inhibit nephrotoxicity induced by cis-platinum (DDP) without inhibition of tumor response in the rat. We report here that DDTC at doses of 25-300 mg/kg inhibits DDP-induced nephrotoxicity and bone marrow toxicity in C57BL/6 X DBA/2F1 (hereafter called B6D2F1) mice, F344 ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Bodenner DL,Dedon PC,Keng PC,Katz JC,Borch RF

    更新日期:1986-06-01 00:00:00