Identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations.

Abstract:

:Presumptive lactic acid bacterial cocci were found in six sourdoughs (out of 20) from the Abruzzo region (central Italy) and subjected to phenotypic and genotypic characterization. A total of 21 isolates, recognized as seven strains by randomly amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) typing, were identified by a polyphasic approach, consisting of 16S rRNA gene sequencing, multiplex PCR assays and physiological features, as Enterococcus faecium and Pediococcus pentosaceus. Four strains belonging to those species and previously isolated from wheat kernels were inoculated in sterile flour to verify their capacity to grow in sourdough environment. Doughs with several dual bacterial combinations, including Lactobacillus sanfranciscensis, were propagated for 11 days and pH measurements and bacterial counts were carried out.

journal_name

Food Microbiol

journal_title

Food microbiology

authors

Corsetti A,Settanni L,Valmorri S,Mastrangelo M,Suzzi G

doi

10.1016/j.fm.2007.01.002

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

592-600

issue

6

eissn

0740-0020

issn

1095-9998

pii

S0740-0020(07)00008-1

journal_volume

24

pub_type

杂志文章