Abstract：
:The pharmacology of excitatory cholinergic responses in CA1 pyramidal cells was examined in detail using intracellular recording from the hippocampal slice preparation. Acetylcholine (ACh), carbachol, muscarine and pilocarpine depolarized the membrane potential with an associated increase in input resistance. In addition, these agonists increased cell firing and depressed the afterhyperpolarization (AHP) that is due to a calcium-activated potassium conductance. The weak effects of ACh (20-200 microM) were considerably enhanced by addition of eserine (1-10 microM). All excitatory effects were completely antagonized by atropine (0.1-1 microM) but unaffected by dihydro-beta-erythroidine (DHBE) and gallamine (1-50 microM). In contrast to the muscarinic agonists, the nicotinic agonists nicotine and dimethylphenylpiperazinium (DMPP) had no excitatory effects on CA1 pyramidal cells. Phenyltrimethylammonium (PTMA), at high concentrations did depolarize cells and depress the AHP but these effects were antagonized by atropine and not DHBE or gallamine. The action of the analogue of cyclic GMP, 8-bromo-cyclic GMP, although variable, mimicked the membrane effects of ACh in some cells and depressed the AHP in most cells. Intracellular injection of cyclic GMP routinely depressed the AHP. In summary, we have demonstrated two cholinergic responses of hippocampal pyramidal cells that are mediated purely by muscarinic receptors. We could find no evidence to support a mixed-type receptor or the involvement of nicotinic receptors in the excitation of hippocampal pyramidal cells to cholinergic agents.
journal_name
Brain Resjournal_title
Brain researchauthors
Cole AE,Nicoll RAdoi
10.1016/0006-8993(84)90434-7subject
Has Abstractpub_date
1984-07-09 00:00:00pages
283-90issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(84)90434-7journal_volume
305pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Ghrelin can alleviate cancer chemotherapy-induced dyspepsia in rodents, though the neural mechanisms involved are not known. Therefore, ghrelin projections from the lateral hypothalamus (LH) and its involvement in the regulation of gastric motility in cisplatin-treated rats were investigated with a multi-disciplined a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.01.004
更新日期：2017-03-15 00:00:00
abstract：:In the following study the behavioural effects of simultaneous lesion of the nucleus basalis magnocellularis (NBM) using ibotenic acid and noradrenergic depletion following a single i.p. administration of DSP4 (50 mg/kg) were examined in the rat. NBM lesion induced a deficit in acquisition of a reinforced T-maze alter...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91067-o
更新日期：1992-11-13 00:00:00
abstract：:We report an fMRI experiment on deductive reasoning with acoustically presented problems. Twelve volunteers received problems in which an acoustic stimulus came from the left or the right of another stimulus. The participants then heard a third stimulus coming from the left or the right of one of the proceeding stimul...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.10.025
更新日期：2009-01-16 00:00:00
abstract：:Newborn rats were given saline or cholecystokinin8 (CCK8) (5 micrograms/kg, twice daily) i.p. for 3 weeks. On day 21, effects on brain development were assessed. CCK-like immunoreactivity was measured in 7 brain regions; a small (12-18%) but significant decrease in endogenous levels of this peptide was detected in cer...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90147-7
更新日期：1987-03-01 00:00:00
abstract：:Ion channels are integral membrane proteins that allow passive diffusion of ions across membranes. In neurons and in other excitable cells, the harmonious coordination between the numerous types of ion channels shape and propagate electrical signals. Increased accumulation of reactive oxidative species (ROS), and subs...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2016.02.046
更新日期：2016-05-15 00:00:00
abstract：:Sensory fibers trapped in nerve-end neuromas become abnormally excitable, and produce an ectopic discharge which is believed to contribute to paresthesias and pain associated with chronic nerve injury in man. Here we report that stimulation of injured nerves can alter this discharge, directly by antidromic invasion of...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90275-7
更新日期：1987-07-07 00:00:00
abstract：:Rapid eye movement (REM) sleep is hypothesized to result from the activity of REM sleep-generating and REM sleep-inhibiting neurons. The serotoninergic (5-HT) neurons of the dorsal raphe nucleus (DRN) represents one such population of REM-sleep inhibiting neurons since they are silent during REM sleep. Consistent with...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03209-7
更新日期：2001-12-27 00:00:00
abstract：:Murine cortical cell cultures deprived of glucose for 6-8 h developed extensive neuronal degeneration, apparent both morphologically and by efflux of lactate dehydrogenase to the bathing medium. This neuronal damage could be substantially reduced by addition of D-2-amino-5-phosphonovalerate (D-APV), in a concentration...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90179-0
更新日期：1989-04-03 00:00:00
abstract：BACKGROUND:Cerebral ischemia is a major leading cause of death and at the first place cause of disability all over the world. There are a lot of drugs that are in experimental stage for treatment of stroke. Among them are calcium channel blockers (CCBs) that have, in animal models, different effectiveness in healing of...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.07.038
更新日期：2007-10-24 00:00:00
abstract：:Hypothalamus and frontal cortex beta-adrenergic receptors, as measured by [125I]cyanopindolol binding, were investigated in male rats both after 4 days of acute starvation and after 2 weeks of semistarvation with dietary manipulation. Moreover, to test whether lymphocyte beta 2-adrenergic receptors can be used as a ma...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91519-3
更新日期：1988-03-01 00:00:00
abstract：:Spontaneous, synchronous burst discharges originating in the CA2-CA3 region of guinea pig hippocampal slices occur when the concentration of magnesium in the perfusate is reduced below 250 microM. The burst frequency is greater than that seen with other convulsants and afterdischarge is common. Cyclic periods of burst...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(87)80042-2
更新日期：1987-04-28 00:00:00
abstract：:Efflux of choline from the rat cerebral cortex in vivo was investigated using the cup technique. After removal of the dura mater, the cup was placed on the cortex. Transmission and scanning electron microscopy revealed that the cortex was separated from the cup solution (100-300 microliter) by basal lamina, pia mater,...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91007-8
更新日期：1985-10-21 00:00:00
abstract：:The effect of electrolytic lesion of the paraventricular nucleus of the hypothalamus (PVN) on yawning and penile erection induced by apomorphine, oxytocin and adrenocorticotropic hormone (ACTH1-24) was studied in male rats. In sham-operated rats, apomorphine (50 micrograms/kg s.c.), oxytocin (30 ng i.c.v.), and ACTH1-...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91305-9
更新日期：1987-09-22 00:00:00
abstract：:Cortical sensory neurons synchronize their activity at multiple frequency bands after an external stimulation. In the somatosensory cortical areas, previous reports describe more discrete and somatotopically specific neural synchronization at the gamma band. Therefore, an efficient gamma synchronization of the neurons...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)03171-8
更新日期：2003-10-03 00:00:00
abstract：:The trisomy-16 mouse is considered to be a model of human trisomy-21 (Down syndrome). We have examined the electrical membrane properties of cultured dorsal root ganglion (DRG) neurons from normal and trisomy-16 fetuses. Trisomy-16 neurons had significantly accelerated rates of action potential depolarization and repo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90678-1
更新日期：1989-04-17 00:00:00
abstract：:We compared changes in levels of substance P-like immunoreactivity (SPLI) in L4-6 dorsal root ganglia (DRG), L4-6 dorsal roots, sciatic nerve, tibial nerve and hind foot skin in rats following resection or crush injury of the sciatic nerve. The initial depletion of SPLI, which occurred in all areas sampled, was simila...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90725-0
更新日期：1986-02-12 00:00:00
abstract：:The effects of amphetamine were examined in a brain stimulation reward paradigm in which response rate was measured across a range of stimulation frequencies. Both low (0.0625 and 0.125 mg/kg) and high (1.0, 2.0 and 4.0 mg/kg) doses of systemic amphetamine decreased the stimulation frequency needed to sustain low rate...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90653-1
更新日期：1988-09-06 00:00:00
abstract：:The effects of forced swimming for 30 min on extracellular 5-hydroxytryptamine (5-HT) levels were examined in five brain regions in rats using in vivo microdialysis. A single dialysis probe was implanted under surgical anesthesia into either the striatum, ventral hippocampus, frontal cortex, amygdala, or lateral septu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00349-u
更新日期：1995-06-05 00:00:00
abstract：:The effect of monocular enucleation on basophilic and metabolic staining in primary (striate) visual cortex has been compared in Old and New World monkeys. Both species show a 30-40% shrinkage of neurons in the layers of dorsal lateral geniculate nucleus receiving axons from the enucleated eye. In striate cortex Old W...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91589-6
更新日期：1985-05-06 00:00:00
abstract：:Axonal and glial cell development within rat optic nerve in which gliogenesis was altered by systemic injection of 5-azacytidine (5-AZ) was examined by quantitative electron microscopy. In neonatal (0-2 days) rat optic nerves, all fibers are premyelinated, and they exhibit a fairly uniform diameter (approximately 0.22...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91436-8
更新日期：1986-08-13 00:00:00
abstract：:Two selectively bred lines of mice, one responding with high (HA) and the other with low analgesia (LA) to 3 min swims at 20 degrees C were compared with respect to naloxone ability to reverse such swim induced pain threshold elevation. Also, the analgesic effect of morphine was tested in both lines of mice. Naloxone ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91380-6
更新日期：1986-11-05 00:00:00
abstract：:The suprachiasmatic nuclei (SCN) contain the major pacemaker for mammalian circadian rhythms. The SCN receive photic input both directly, via the retinohypothalamic tract (RHT), and indirectly, via the geniculohypothalamic tract (GHT), which originates in cells in the intergeniculate leaflet (IGL) and anterior portion...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91163-3
更新日期：1989-07-31 00:00:00
abstract：:The fine structure of reaggregating cultures of cells from 6- to 7-day-old mouse cerebellum was studied at intervals between 3 and 21 days in vitro (DIV). The resulting aggregates consisted mainly of small neurons (granule, stellate and basket cells), neuroglial cells and their processes. Large neurons were rarely pre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(84)90067-1
更新日期：1984-09-01 00:00:00
abstract：:Amphetamine releases dopamine through a transporter-mediated mechanism. The purpose of this report was to further our understanding of the intracellular pool from which amphetamine releases dopamine: the cytoplasmic pool, the vesicular pool, or both. Rats were treated with D-amphetamine (AMPH) (1.0 or 10.0 mg/kg) or a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00720-3
更新日期：1998-09-21 00:00:00
abstract：:Using nerve growth factor (NGF), anti-NGF sera and dissociated neonatal mouse dorsal root ganglionic neurons we present a microculture assay methodology for (1) the titration of neurotrophic factor (NTF) activity in monolayer culture, (2) the titration of NTF antibodies which 'block' NTF biological activity, (3) the t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90408-x
更新日期：1981-12-28 00:00:00
abstract：:Immunohistochemistry for three calcium-binding proteins calbindin D-28k, calretinin, and parvalbumin revealed neuronal heterogeneity within the GP. Each neurone appeared to express either a single type of calcium binding protein or none at all. The co-localisation of calcium binding proteins was not observed. Combined...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03263-2
更新日期：2002-03-08 00:00:00
abstract：:After spinal cord injury (SCI), the rapidly upregulated chondroitin sulfate proteoglycans (CSPGs), the prominent chemical constituents and main repulsive factors of the glial scar, play an important role in the extremely limited ability to regenerate in adult mammals. Although many methods to overcome the inhibition h...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.08.048
更新日期：2014-10-24 00:00:00
abstract：:The intergeniculate leaflet (IGL) is a distinct division of the lateral geniculate complex that participates in the regulation of the circadian rhythm through its projections to the circadian pacemaker located in the suprachiasmatic nuclei of the hypothalamus. A high number of neuropeptide Y (NPY) cell bodies has been...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)01032-4
更新日期：1998-11-30 00:00:00
abstract：:We have shown previously that progesterone linked with bovine serum albumin at position 3 of the progesterone molecule (P-3-BSA) was effective in modulating (both stimulating and inhibiting) amphetamine (AMPH)-evoked dopamine (DA) release in vitro from superfused corpus striatal (CS) tissue fragments of ovariectomized...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90395-c
更新日期：1991-01-04 00:00:00
abstract：:The adult rat retina was evaluated following surgical lesion and after transplantation of embryonic day 15 (E15) retinal tissue. While a surgical incision caused degeneration and ganglion cell death peripheral to lesion site, the addition of an embryonic retinal implant to the lesion site resulted in a reduction of th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90300-4
更新日期：1989-10-23 00:00:00
