1H-nuclear magnetic resonance of intercalators and rGCA: a potential mutagenicity probe.

Abstract:

:Variable temperature 1H-nuclear magnetic resonance (NMR) has been used to study the interaction of the RNA trimer, GpCpA, with the intercalators ethidium bromide and the acridine derivatives; proflavin, 9-amino-acridine, acridine orange, acridine yellow and acriflavin. The complexes formed were studied at nucleic acid to drug ratios of 1:1 and 5:1, the latter being useful in defining the effects of structural variation in the acridine series and in determining the site of intercalation. All the intercalators greatly stabilized the oligonucleotide duplex, the average melting temperature (Tm) increasing by up to 30 degrees C. Significant changes in individual Tms and chemical shifts were observed for all the GpCpA protons. 9-Amino-acridine and acriflavin did not stabilize the GpCpA duplex as substantially as the other acridine derivatives. It is suggested that this intercalator:GpCpA system, and its associated NMR-derived Tm, is a useful physical probe for potential mutagens.

journal_name

Chem Biol Interact

authors

Coddington JM,Alkema D,Bell RA,Hughes DW,Neilson T

doi

10.1016/0009-2797(84)90135-2

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

97-110

issue

1

eissn

0009-2797

issn

1872-7786

pii

0009-2797(84)90135-2

journal_volume

50

pub_type

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