Pyrosequencing of a recA gene variable region for Burkholderia cepacia complex genomovar identification.

Abstract:

:We developed an assay to identify Burkholderia cepacia complex genomovars by Pyrosequencing of a variable recA gene segment. Fifteen reference strains and 30 clinical isolates of B. cepacia complex were sequenced. Full 77-base pair target sequences were obtained from 44 of the 45 isolates, and BLAST queries of the sequences correctly identified the genomovar of these 44 isolates. Three Burkholderia multivorans isolates were identified as B. multivorans/Burkholderia ambifaria, indicating that additional identification methods may be needed for some B. multivorans strains.

authors

Slinger R,Yan L,Myers R,Ramotar K,St Denis M,Aaron SD

doi

10.1016/j.diagmicrobio.2007.02.011

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

379-84

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(07)00099-5

journal_volume

58

pub_type

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