Electron microscopy of renal and ocular changes in virus-induced diabetes mellitus in mice.


:The eyes, kidneys and pancreas of mice (SJL/J) infected with encephalomyocarditis virus were examined by light and electron microscopy. Diabetic mice with the longest duration (6 months) of diabetes showed marked renal and ocular alterations. Fasting blood glucose levels were 17.8-21.9 mmol/l and glycosuria was present. Clinically, based on ophthalmoscopy and fluorescein angiography, retinal vessels were normal. Histologically, moderately decreased numbers of pericytes were noted following trypsin digestion. The basement membrane of inner retinal vessels showed significant thickening in diabetic mice. Corneal epithelial oedema was present and surface microvillus projections were decreased compared with control mice. The kidneys of the same animals showed nodular and diffuse glomerulosclerosis and mesangial thickening. Electron microscopy showed excessive accumulation of basement membrane-like material in the mesangium and the peripheral glomerular region. Histologically, moderate to advanced kidney disease was associated with relatively early retinopathy.






Rodrigues M,Currier C,Yoon J




Has Abstract


1983-04-01 00:00:00












  • Zinc sulphate induces metallothionein in pancreatic islets of mice and protects against diabetes induced by multiple low doses of streptozotocin.

    abstract:AIMS/HYPOTHESIS:Diabetes is induced by multiple low doses of streptozotocin (MLD-STZ) in male mice of susceptible strains. In this model beta-cell injury and T-cell-mediated inflammatory reactions are induced. Probably, reactive oxygen species (ROS) participate in the destruction of beta cells. The effects of ROS can b...


    pub_type: 杂志文章


    authors: Ohly P,Dohle C,Abel J,Seissler J,Gleichmann H

    更新日期:2000-08-01 00:00:00

  • Effect of metergoline, a powerful and long-acting antiserotoninergic agent, on insulin secretion in normal subjects and in patients with chemical diabetes.

    abstract::The effect of metergoline on insulin secretion has been evaluated in normal subjects and in patients with chemical diabetes. The repeated administration of metergoline, 2 mg at four-hour intervals to give a total of 24 mg, has enhanced insulin secretion in response to i.v. glucose in normal subjects but not in chemica...


    pub_type: 杂志文章


    authors: Pontiroli AE,Viberti GC,Tognetti A,Pozza G

    更新日期:1975-04-01 00:00:00

  • Genetic heterogeneity in the major histocompatibility complex of various BB rat sublines.

    abstract::Fragments of cloned rat class I transplantation antigen genes were used to define the polymorphism detected between two lines of closely related BB rats. One line, BB-Hagedorn (BB/H), is prone to diabetes, and the other (BB control) is resistant. A cDNA probe representing part of the second extracellular domain of a c...


    pub_type: 杂志文章


    authors: Kryspin-Sørensen I,Dyrberg T,Kastern W

    更新日期:1986-05-01 00:00:00

  • A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans.

    abstract:AIMS/HYPOTHESIS:We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans. METHODS:We studied pancreases obtained at autopsy from individuals (n = 11) who had prior opposite-sex cord blood transplants to reconstitute hae...


    pub_type: 杂志文章


    authors: Huang CJ,Butler AE,Moran A,Rao PN,Wagner JE,Blazar BR,Rizza RA,Manivel JC,Butler PC

    更新日期:2011-05-01 00:00:00

  • Effect of experimental diabetes and glucagon on cAMP-dependent protein kinase in rat liver.

    abstract::Liver protein kinase was determined in the absence and presence of cAMP4. Experimental alloxan diabetes resulted in a decrease in total protein kinase (+cAMP) and an increase in the activity ratio (-cAMP) divided by (+cAMP) in liver. Insulin treatment of diabetic rats reversed the observed changes in protein kinase in...


    pub_type: 杂志文章


    authors: Weber HE,Menahan LA,Chaudhuri SN,Shipp JC

    更新日期:1977-04-01 00:00:00

  • Outcomes of surgical treatment of diabetic foot osteomyelitis: a series of 185 patients with histopathological confirmation of bone involvement.

    abstract:AIMS/HYPOTHESIS:We analysed the factors that determine the outcomes of surgical treatment of osteomyelitis of the foot in diabetic patients given early surgical treatment within 12 h of admission and treated with prioritisation of foot-sparing surgery and avoidance of amputation. METHODS:A consecutive series of 185 di...


    pub_type: 杂志文章


    authors: Aragón-Sánchez FJ,Cabrera-Galván JJ,Quintana-Marrero Y,Hernández-Herrero MJ,Lázaro-Martínez JL,García-Morales E,Beneit-Montesinos JV,Armstrong DG

    更新日期:2008-11-01 00:00:00

  • Mutation at position -132 in the islet amyloid polypeptide ( IAPP) gene promoter enhances basal transcriptional activity through a new CRE-like binding site.

    abstract:AIMS/HYPOTHESIS:Mutations in the islet amyloid polypeptide ( IAPP) gene may play a potential role in the abnormal regulation or expression of the peptide. The aim of this study was to determine the functional role of the -132 G/A mutation reported in the promoter region of the IAPP gene in a population of Spanish Type ...


    pub_type: 杂志文章


    authors: Novials A,Mato E,Lucas M,Franco C,Rivas M,Santisteban P,Gomis R

    更新日期:2004-07-01 00:00:00

  • A genetic variant of G6PC2 is associated with type 2 diabetes and fasting plasma glucose level in the Chinese population.

    abstract:AIMS/HYPOTHESIS:Single nucleotide polymorphisms (SNPs) in G6PC2 have been reported to be associated with fasting plasma glucose level in several populations of European descent. However, whether G6PC2 variants have a similar effect in other ethnic groups is unknown. The aim of this study was to investigate the effect o...


    pub_type: 杂志文章


    authors: Hu C,Zhang R,Wang C,Ma X,Wang C,Fang Q,Bao Y,Xiang K,Jia W

    更新日期:2009-03-01 00:00:00

  • Islet cell and insulin autoantibodies in organ-specific autoimmune patients. Their behaviour and predictive value for the development of type 1 (insulin-dependent) diabetes mellitus. A 10-year follow-up study.

    abstract::To evaluate the behaviour and predictive value of islet cell and insulin autoantibodies in patients with organ-specific autoimmune diseases, we followed 21 non-diabetic subjects for a mean period of 84 +/- 27 months. Ten patients were persistently seropositive for complement-fixing islet cell antibodies and high titre...


    pub_type: 杂志文章


    authors: Betterle C,Presotto F,Pedini B,Moro L,Slack RS,Zanette F,Zanchetta R

    更新日期:1987-05-01 00:00:00

  • Identification of four trp1 gene variants murine pancreatic beta-cells.

    abstract::Insulin secretion is stimulated by glucose, hormones and neurotransmitters. Both activation of a non-selective cation current and activation of a Ca2+ current in response to depletion of intracellular Ca2+ stores have been suggested to play a role in this stimulation. The properties of these currents resemble those re...


    pub_type: 杂志文章


    authors: Sakura H,Ashcroft FM

    更新日期:1997-05-01 00:00:00

  • Kidney transplantation in type 1 (insulin-dependent) diabetic patients. Early glomerulopathy.

    abstract::The development of diabetic glomerulopathy in kidneys transplanted to diabetic patients was estimated in transplant biopsies and evaluated in relation to suspected clinical risk factors for diabetic nephropathy. Surgical biopsies were taken at baseline and at 24-36 months post-transplantation in 16 Type 1 (insulin-dep...


    pub_type: 杂志文章


    authors: Osterby R,Nyberg G,Hedman L,Karlberg I,Persson H,Svalander C

    更新日期:1991-09-01 00:00:00

  • Retinal vascular fractals predict long-term microvascular complications in type 1 diabetes mellitus: the Danish Cohort of Pediatric Diabetes 1987 (DCPD1987).

    abstract:AIMS/HYPOTHESIS:Fractal analysis of the retinal vasculature provides a global measure of the complexity and density of retinal vessels summarised as a single variable: the fractal dimension. We investigated fractal dimensions as long-term predictors of microvasculopathy in type 1 diabetes. METHODS:We included 180 pati...


    pub_type: 杂志文章


    authors: Broe R,Rasmussen ML,Frydkjaer-Olsen U,Olsen BS,Mortensen HB,Peto T,Grauslund J

    更新日期:2014-10-01 00:00:00

  • Target tissue production and axonal transport of neurotrophin-3 are reduced in streptozotocin-diabetic rats.

    abstract::Neurotrophin-3 (NT-3) acts as a target-derived neurotrophic factor for large calibre sensory neurones and plays a role in the maintenance of the adult phenotype of proprioceptive and mechanoreceptive fibres. Large fibre sensory neuropathy is common in diabetes mellitus and the aim of this study was to determine whethe...


    pub_type: 杂志文章


    authors: Fernyhough P,Diemel LT,Tomlinson DR

    更新日期:1998-03-01 00:00:00

  • Blood flow in the diabetic neuropathic foot.

    abstract::The mechanisms which underlie the development of Charcot joints and foot ulceration are poorly understood. The present study using non-invasive Doppler techniques demonstrates that in the neuropathic leg, the arteries are rigid, peripheral blood flow is increased and associated with arteriovenous shunting. We studied ...


    pub_type: 杂志文章


    authors: Edmonds ME,Roberts VC,Watkins PJ

    更新日期:1982-01-01 00:00:00

  • DEXI, a candidate gene for type 1 diabetes, modulates rat and human pancreatic beta cell inflammation via regulation of the type I IFN/STAT signalling pathway.

    abstract:AIMS/HYPOTHESIS:The initial stages of type 1 diabetes are characterised by an aberrant islet inflammation that is in part regulated by the interaction between type 1 diabetes susceptibility genes and environmental factors. Chromosome 16p13 is associated with type 1 diabetes and CLEC16A is thought to be the aetiological...


    pub_type: 杂志文章


    authors: Dos Santos RS,Marroqui L,Velayos T,Olazagoitia-Garmendia A,Jauregi-Miguel A,Castellanos-Rubio A,Eizirik DL,Castaño L,Santin I

    更新日期:2019-03-01 00:00:00

  • Functional alterations of G-proteins in diabetic rat retina: a possible explanation for the early visual abnormalities in diabetes mellitus.

    abstract::We examined changes in guanosine triphosphate-dependent signal transduction mechanisms in the retina from the early stages of the streptozotocin-diabetic rat, a model for Type 1 (insulin-dependent) diabetes mellitus. Guanosine triphosphate binding, guanosine triphosphatase activity, and binding of (azido) guanosine tr...


    pub_type: 杂志文章


    authors: Kowluru A,Kowluru RA,Yamazaki A

    更新日期:1992-07-01 00:00:00

  • High amniotic fluid erythropoietin levels are associated with an increased frequency of fetal and neonatal morbidity in type 1 diabetic pregnancies.

    abstract:AIMS/HYPOTHESIS:In this study we investigated whether chronic fetal hypoxia, as indicated by amniotic fluid erythropoietin levels, is associated with perinatal morbidity in type 1 diabetic pregnancies. METHODS:A total of 331 women with type 1 diabetes had at least one childbirth between 1995 and 2000. The amniotic flu...


    pub_type: 杂志文章


    authors: Teramo K,Kari MA,Eronen M,Markkanen H,Hiilesmaa V

    更新日期:2004-10-01 00:00:00

  • Major lower limb amputation in diabetic patients: development during 1982 to 1993.

    abstract::This study analyses 2848 primary major lower limb amputations in diabetic individuals during the period 1982-1993. The male:female sex ratio (mean 1.18) increased, while the mean age at the primary amputation was constant (70.96 years) during the period. The incidence uniformly decreased for both men and women by 0.96...


    pub_type: 杂志文章


    authors: Ebskov B,Ebskov L

    更新日期:1996-12-01 00:00:00

  • Correction to: Chronomedicine and type 2 diabetes: shining some light on melatonin.

    abstract::The authors have been made aware that the following sentence is incorrect: 'Like IIK7, both ramelteon and tasimelteon have a greater affinity for the MT2 receptor [162].' ...


    pub_type: 杂志文章,已发布勘误


    authors: Forrestel AC,Miedlich SU,Yurcheshen M,Wittlin SD,Sellix MT

    更新日期:2018-05-01 00:00:00

  • Hypoxia and hypoxia-inducible factors in diabetes and its complications.

    abstract::Hypoxia-inducible factors (HIFs) are the key regulators of oxygen homeostasis in response to hypoxia. In diabetes, multiple tissues are hypoxic but adaptive responses to hypoxia are impaired due to insufficient activation of HIF signalling, which results from inhibition of HIF-1α stability and function due to hypergly...


    pub_type: 杂志文章,评审


    authors: Catrina SB,Zheng X

    更新日期:2021-01-26 00:00:00

  • An oxidative stress paradox: time for a conceptual change?

    abstract::Oxidative stress has long been considered a key driving factor of many obesity-related health problems. However, recent work by Merry, Tran et al (Diabetologia DOI 10.1007/s00125-016-4084-3 ) challenges this idea with an interesting study using a hepatocyte-specific Gpx1-knockout (HGKO) mouse. GPX1 is an important det...


    pub_type: 评论,社论


    authors: Haas JT,Staels B

    更新日期:2016-12-01 00:00:00

  • Best practice guidelines for the molecular genetic diagnosis of maturity-onset diabetes of the young.

    abstract:AIMS/HYPOTHESIS:Mutations in the GCK and HNF1A genes are the most common cause of the monogenic forms of diabetes known as 'maturity-onset diabetes of the young'. GCK encodes the glucokinase enzyme, which acts as the pancreatic glucose sensor, and mutations result in stable, mild fasting hyperglycaemia. A progressive i...


    pub_type: 杂志文章


    authors: Ellard S,Bellanné-Chantelot C,Hattersley AT,European Molecular Genetics Quality Network (EMQN) MODY group.

    更新日期:2008-04-01 00:00:00

  • Effects of insulin on the disposal of 14C-labelled very low density lipoprotein triglycerides in intact and hepatectomized rats.

    abstract::In sham-operated rats, intravenous administration of 14C-very low density lipoprotein triglycerides (with labelled esterified fatty acids) caused an initial decrease and subsequent increase in plasma 14C-lipids of both very low density lipoproteins (VLDL) (density less than 1.006) and lipoproteins of density greater t...


    pub_type: 杂志文章


    authors: Argilés J,Herrera E

    更新日期:1983-04-01 00:00:00

  • Heterogeneous nuclear ribonucleoproteins F and K mediate insulin inhibition of renal angiotensinogen gene expression and prevention of hypertension and kidney injury in diabetic mice.

    abstract:AIMS/HYPOTHESIS:We investigated whether heterogeneous nuclear ribonucleoproteins F and K (hnRNP F, hnRNP K) mediate insulin inhibition of renal Agt expression and prevention of hypertension and kidney injury in an Akita mouse model of type 1 diabetes. METHODS:Adult male Akita mice (12 weeks old) were treated with insu...


    pub_type: 杂志文章


    authors: Abdo S,Lo CS,Chenier I,Shamsuyarova A,Filep JG,Ingelfinger JR,Zhang SL,Chan JS

    更新日期:2013-07-01 00:00:00

  • Non-neural tyrosine hydroxylase, via modulation of endocrine pancreatic precursors, is required for normal development of beta cells in the mouse pancreas.

    abstract:AIMS/HYPOTHESIS:Apart from transcription factors, little is known about the molecules that modulate the proliferation and differentiation of pancreatic endocrine cells. The early expression of tyrosine hydroxylase (TH) in a subset of glucagon(+) cells led us to investigate whether catecholamines have a role in beta cel...


    pub_type: 杂志文章


    authors: Vázquez P,Robles AM,de Pablo F,Hernández-Sánchez C

    更新日期:2014-11-01 00:00:00

  • Elevated von Willebrand factor antigen predicts deterioration in diabetic peripheral nerve function.

    abstract::We have studied the temporal relationship of plasma von Willebrand Factor (vWF), a marker of endothelial damage, with the development of complications in 63 young diabetic patients (56 of whom were insulin-dependent) who took part in a prospective study. Results are presented from baseline to follow-up. In the group a...


    pub_type: 杂志文章


    authors: Plater ME,Ford I,Dent MT,Preston FE,Ward JD

    更新日期:1996-03-01 00:00:00

  • Type 1 and type 2 diabetes after gestational diabetes: a 23 year cohort study.

    abstract:AIMS/HYPOTHESIS:The aim of this work was to examine the progression to type 1 and type 2 diabetes after gestational diabetes mellitus (GDM) in a 23 year follow-up study. METHODS:We carried out a cohort study of 391 women with GDM diagnosed by an OGTT or the use of insulin treatment during pregnancy, and 391 age- and p...


    pub_type: 杂志文章


    authors: Auvinen AM,Luiro K,Jokelainen J,Järvelä I,Knip M,Auvinen J,Tapanainen JS

    更新日期:2020-10-01 00:00:00

  • The clinical application of genetic testing in type 2 diabetes: a patient and physician survey.

    abstract:AIMS/HYPOTHESIS:Advances in type 2 diabetes genetics have raised hopes that genetic testing will improve disease prediction, prevention and treatment. Little is known about current physician and patient views regarding type 2 diabetes genetic testing. We hypothesised that physician and patient views would differ regard...


    pub_type: 杂志文章


    authors: Grant RW,Hivert M,Pandiscio JC,Florez JC,Nathan DM,Meigs JB

    更新日期:2009-11-01 00:00:00

  • Glucocorticoids and the fate of exogenous insulin in insulin-immunized guinea pigs.

    abstract::Groups of normal rats and of normal and insulin-immunized guinea pigs were injected intravasculary with mixtures of radio-iodinated and unlabelled porcine insulins. No differences could be discerned between the fates of radio-iodinated and unlabelled insulins in normal or immune guinea pigs except with respect to the ...


    pub_type: 杂志文章


    authors: Wright PH,Gingerich RL,Oliver JR

    更新日期:1975-08-01 00:00:00

  • Myofibroblast progenitor cells are increased in number in patients with type 1 diabetes and express less bone morphogenetic protein 6: a novel clue to adverse tissue remodelling?

    abstract:AIMS/HYPOTHESIS:Growth factor imbalance and endothelial progenitor cell dysfunction are well-known elements of the inappropriate response to injury in human and experimental diabetes. We hypothesised that in diabetes the outgrowth of myofibroblast progenitor cells (MFPCs) is also altered and that this relates to aberra...


    pub_type: 杂志文章


    authors: Nguyen TQ,Chon H,van Nieuwenhoven FA,Braam B,Verhaar MC,Goldschmeding R

    更新日期:2006-05-01 00:00:00