Abstract:
:The multi-domain neutral endopeptidase, ADAMTS-1 (a disintegrin and metalloprotease with thrombospondin repeats) is induced by parathyroid hormone (PTH) in rat osteoblasts and has therefore been suggested to be involved in initiation of bone remodeling. However, its function(s) in bone cells have not been studied. Here, we first establish that ADAMTS-1 protein is rapidly and transiently produced by human primary osteoblasts in response to PTH (1-34). We also show that ADAMTS-1 is specifically in close proximity to collagen fibrils in bone tissue using ultrastructural immunolabeling. To study the consequence(s) of ADAMTS-1 metalloprotease production in osteoblastic cells, human osteosarcoma cells (SaOS-2), were forced to express either wild-type (wtATS) or a point-mutated (pmATS) metalloprotease dead ADAMTS-1. SaOS-2 cells expressing wtATS had a growth advantage and increased collagenolytic activity when seeded inside a collagen type I gel but exhibited a reduced migration in a scratch wound assay. Immunolabeling of moving cells shows ADAMTS-1 to be located towards the direction of cellular migration. Finally, Western analysis demonstrated excess accumulation of mature collagen type I alpha1 species in the extracellular matrix together with increased release of distinct small collagen fragments into the conditioned media, by cultures of wtATS cells compared to pmATS cells. These results show that ADAMTS-1 has both the opportunity in bone and capability in vitro to induce collagen type I processing, together with a positive influence on osteoblastic three-dimensional growth. Although it is not clear at present if ADAMTS-1 promotes collagen degradation directly or indirectly, it shows that ADAMTS-1 activity can have a profound influence on the osteoblast phenotype, inhibiting migration on a planar substrate but enhancing growth in a collagen scaffold. These findings further establish ADAMTS-1 as a potentially important protein in PTH induced bone remodeling.
journal_name
Bonejournal_title
Boneauthors
Rehn AP,Birch MA,Karlström E,Wendel M,Lind Tdoi
10.1016/j.bone.2007.04.187subject
Has Abstractpub_date
2007-08-01 00:00:00pages
231-8issue
2eissn
8756-3282issn
1873-2763pii
S8756-3282(07)00371-7journal_volume
41pub_type
杂志文章相关文献
BONE文献大全abstract::Treatment with parathyroid hormone 1-84 (PTH) or teriparatide increases osteonal remodeling and decreases bone mineral density (BMD) at cortical (Ct) bone sites but may also increase bone size. Decreases in BMD and increases in size exert opposing effects on bone strength. In adult ovariectomized (OVX) rhesus monkeys,...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.04.197
更新日期:2007-09-01 00:00:00
abstract::In this study, we observed mitotic preosteoblasts that have the structural features of osteoblasts in the primary spongiosa of the rat mandibular condyle. The rough endoplasmic reticulum and the Golgi apparatus showed remarkable disorganization during mitosis. The Golgi saccules were replaced by groups of large vacuol...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(93)90253-7
更新日期:1993-01-01 00:00:00
abstract:INTRODUCTION:The GH-IGF axis has profound effects on bone metabolism and may be important in the etiology of idiopathic osteoporosis. Serum IGF-I is often low in men with osteoporosis, which may be attributable to GH hypo-secretion or hepatic GH insensitivity. We studied the GH-IGF axis in depth to look for evidence to...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.06.016
更新日期:2005-12-01 00:00:00
abstract::Bisphosphonates play an important role in the treatment of metabolic bone diseases such as osteoporosis. In addition to their anti-resorptive activity by triggering osteoclast apoptosis, nitrogen-containing bisphosphonates (N-BP) may also influence osteogenic differentiation, which might rely on their capacity to inhi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.03.020
更新日期:2018-06-01 00:00:00
abstract::Bisphosphonates (BPs), bone targeted drugs that disrupt osteoclast function, are routinely used to treat complications of bone metastasis. Studies in preclinical models of cancer have shown that BPs reduce skeletal tumor burden and increase survival. Similarly, we observed in the present study that administration of t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.01.010
更新日期:2009-05-01 00:00:00
abstract::Bisphosphonates increase bone mass and reduce fracture risk, but their anti-resorptive action may lead to increases in fatigue microdamage. To investigate how bisphosphonate effects influence changes in bone volume and microdamage in the long term, a strain-adaptive model of bone remodeling and microdamage balance was...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.03.007
更新日期:2004-07-01 00:00:00
abstract::Osteoporosis is a major cause of disability in the United States. Numerous factors contribute to the decline in bone mineral density (BMD) that characterizes this disease, and the importance of heredity is now widely appreciated. We evaluated the joint contributions of genes and environmental factors on variation in B...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00246-1
更新日期:2003-11-01 00:00:00
abstract:CASE REPORT:A female patient with consanguineous parents presented with severe symptomatic hypocalcemia (1.62mmol/l) at the age of 4 months. Treatment with oral 1,25-(OH)2-vitamin D and calcium carbonate was started and serum calcium concentrations were stabilized at the lower end of the normal range. Subsequently she ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.06.009
更新日期:2012-09-01 00:00:00
abstract::Bone sialoprotein (BSP) is highly expressed in early bone deposition and may play a part in primary bone mineralization. We previously showed that while BSP−/− mice have a mild secondary bone phenotype and are responsive to mechanical (unloading) and hormonal (ovariectomy, parathyroid hormone (PTH)) challenges, repair...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.02.014
更新日期:2012-05-01 00:00:00
abstract::Recently, the involvement of immune responses in metabolic bone disease and/or local bone destruction has received much attention. Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), a member of the immunoglobulin (Ig) superfamily, negatively regulates T cell activation. The deficiency of CTLA-4 induces profound ost...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.06.018
更新日期:2004-11-01 00:00:00
abstract::Mutations in the SCN8A gene encoding the neuronal voltage-gated sodium channel Nav1.6 are known to be associated with epileptic encephalopathy type 13. We identified a novel de novo SCN8A mutation (p.Phe360Ala, c.1078_1079delTTinsGC, Exon 9) in a 6-year-old girl with epileptic encephalopathy accompanied by severe juve...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.06.025
更新日期:2017-10-01 00:00:00
abstract::Anterior cruciate ligament (ACL) tears are a common sports-related knee injury that increases the risk of developing post-traumatic osteoarthritis (OA). During OA progression bone microarchitecture changes in the affected knee, however, little is known about bone microarchitecture in knees with early stage OA. The pur...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.03.006
更新日期:2018-06-01 00:00:00
abstract::Popular psychotropic drugs, like the antidepressant selective serotonin reuptake inhibitors (SSRIs) and tricyclic antidepressants (TCAs), and the mood stabilizer lithium, may have skeletal effects. In particular, preclinical observations suggest a direct negative effect of SSRIs on the skeleton. A potential caveat in ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.12.031
更新日期:2010-04-01 00:00:00
abstract::Profound bone loss at weight bearing sites is a primary effect of long-duration spaceflight. Moreover, a significant increase in estimated fracture risk remains even 1 year after returning to Earth; hence, it is important to define how quickly bone integrity can recover following prolonged disuse. This study character...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.09.021
更新日期:2013-01-01 00:00:00
abstract::Glucocorticoids (GC) are used for the treatment of a wide spectrum of diseases because of their potent anti-inflammatory and immunosuppressive effects, and they are serious and common causes of secondary osteoporosis. Administration of intermittent parathyroid hormone (PTH) may induce formation of new bone and may cou...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.01.142
更新日期:2006-08-01 00:00:00
abstract::Two major mechanisms that could potentially be responsible for toughening in mineralized tissues, such as bone and dentin, have been identified-microcracking and crack bridging. While evidence has been reported for both mechanisms, there has been no consensus thus far on which mechanism plays the dominant role in toug...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.02.001
更新日期:2004-05-01 00:00:00
abstract::Bone is a complex endocrine organ that facilitates structural support, protection to vital organs, sites for hematopoiesis, and calcium homeostasis. The bone marrow microenvironment is a heterogeneous niche consisting of multipotent musculoskeletal and hematopoietic progenitors and their derivative terminal cell types...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2018.05.019
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:To assess the ability of the Spanish version of the WHO fracture risk assessment tool (FRAX®) to predict the observed incident fractures in the ECOSAP Study cohort. METHODS:5201 women, aged 65 or older, were enrolled in a three-year, prospective study by a non-randomized sampling of consecutive cases in 58 pri...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.11.006
更新日期:2012-01-01 00:00:00
abstract::Paget's disease is the most exaggerated example of bone remodeling in which abnormal osteoclastic bone resorption remains coupled to new bone formation. There are abnormalities in the stages of osteoclast development, and studies in Paget's disease have suggested a major role for IL-6 in human osteoclast activity. The...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/8756-3282(96)00211-6
更新日期:1996-09-01 00:00:00
abstract::Pre-clinical in vivo models of multiple myeloma are essential tools for investigating the pathophysiology of multiple myeloma and for testing new therapeutic agents and strategies prior to their potential use in clinical trials. Over the last five decades, several different types of murine models of multiple myeloma h...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2015.04.004
更新日期:2015-08-01 00:00:00
abstract::Osteoporosis is the consequence of altered bone metabolism resulting in the systemic reduction of bone strength and increased risk of fragility fractures. MicroRNAs (miRNAs) regulate gene expression on a post-transcriptional level and are known to take part in the control of bone formation and bone resorption. In addi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.05.027
更新日期:2015-10-01 00:00:00
abstract::This study investigated the possible role in vitro of insulin-like growth factor I (IGF-I) as a mediator of the effects of growth hormone (GH) on osteoclastic resorption in an unfractioned rabbit bone cell model. After 4 days of rabbit bone cell culture, human GH (hGH) (50 ng/mL) and human IGF-I (hIGF-I) (50 ng/mL) si...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00224-x
更新日期:1998-01-01 00:00:00
abstract::The variability of different primary tumors in the susceptibility to metastatic bone disease is poorly understood. Factors that determine the viability of metastatic cells are also poorly understood, but may depend in part upon gene expression of PTHrP and the vitamin D receptor. In contrast, much more is known of the...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/8756-3282(95)00194-i
更新日期:1995-08-01 00:00:00
abstract::To better understand the effects of prolonged bed-rest in women, 24 healthy women aged 25 to 40 years participated in 60-days of strict 6° head-down tilt bed-rest (WISE-2005). Subjects were assigned to either a control group (CON, n=8) which performed no countermeasure, an exercise group (EXE, n=8) undertaking a combi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.06.021
更新日期:2011-10-01 00:00:00
abstract:AIM:Factorial design was used to test our hypothesis whether a group of flavonoids (FE) derived from herbal Epimedium Brevicornum Maxim exerted its preventive effects on estrogen-deficiency-induced osteoporosis mainly through an enhancement in intestinal calcium absorption. MATERIALS AND METHODS:Forty-five 12-month-ol...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.11.019
更新日期:2006-06-01 00:00:00
abstract::Microdeletions within 1q24 have been associated with growth deficiency, varying intellectual disability, and skeletal abnormalities. The candidate locus responsible for the various phenotypic features of this syndrome has previously been predicted to lie in the area of 1q24.3, but molecular evidence of the causative g...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115705
更新日期:2021-01-01 00:00:00
abstract::Bisphosphonate (BP)-related osteonecrosis of the jaw, previously known as BRONJ, now referred to more broadly as medication-related osteonecrosis of the jaw (MRONJ), is a morbid condition that represents a significant risk for oncology patients who have received high dose intravenous (IV) infusion of a potent nitrogen...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.03.027
更新日期:2019-06-01 00:00:00
abstract::About 1 in 10 postmenopausal UK women are currently prescribed oral bisphosphonates, but there are concerns about their adverse effects. Osteonecrosis of the jaw is a recognised uncommon but important side effect of intravenous bisphosphonates, but epidemiological evidence on risk of osteonecrosis of the jaw associate...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.04.003
更新日期:2019-07-01 00:00:00
abstract::Fracture healing is a regenerative process in which bone is restored without scar tissue formation. The healing cascade initiates with a cycle of inflammation, cell migration, proliferation and differentiation. Immune cells invade the fracture site immediately upon bone damage and contribute to the initial phase of th...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.03.052
更新日期:2014-07-01 00:00:00
abstract:INTRODUCTION:Because osteoporotic patients are prone to fractures, it must be considered whether or not patients undergoing drug therapies should discontinue treatment after sustaining a non-vertebral fracture. This study has tested the effect of novel active vitamin D3 analog, 1alpha,25-dihydroxy-2beta(3-hydroxypropox...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.07.023
更新日期:2007-01-01 00:00:00