Comparison of complement fixation and fluorescent immunoassay (FIAX) for measuring antibodies to cytomegalovirus and herpes simplex virus.

Abstract:

:An automated fluorescent immunoassay system (FIAX) was compared with complement fixation (CF) for measuring antibodies to cytomegalovirus (CMV) and herpes simplex virus (HSV) in clinical specimens. Compared with CF the specificity of FIAX (ability to detect a negative titer) was 95% for CMV and 92% for HSV. The sensitivity of FIAX (ability to detect a positive titer) was 98% for CMV and 95% for HSV. When paired samples were tested for significant titer rises, the concordance of the two assays for CMV was 96% and for HSV 80%. The FIAX assay was found to be reproducible when sera were tested within the same laboratory and in two different laboratories. The assay is fast, simple to perform, and not affected by anticomplementary activity. It is seen as a useful alternative to CF testing in a clinical virology laboratory.

journal_name

Am J Clin Pathol

authors

Friedman HM,Tustin NB,Hitchings MM,Plotkin SA

doi

10.1093/ajcp/76.3.305

subject

Has Abstract

pub_date

1981-09-01 00:00:00

pages

305-7

issue

3

eissn

0002-9173

issn

1943-7722

journal_volume

76

pub_type

杂志文章
  • Misleading hepatitis C serology following administration of intravenous immunoglobulin.

    abstract::Intravenous immunoglobulin (IVIG) is a blood derivative that is being evaluated for the prevention and treatment of numerous disorders. Recent studies have suggested beneficial effects from the prophylactic administration of IVIG to very-low-birth-weight neonates. A disadvantage of such therapy is misleading infectiou...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.3.327

    authors: Nixon RR,Smith SA,Johnson RL,Pillers DA

    更新日期:1994-03-01 00:00:00

  • Anaplastic thyroid carcinoma. Immunocytochemical study of 32 cases.

    abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.1.15

    authors: Ordóñez NG,El-Naggar AK,Hickey RC,Samaan NA

    更新日期:1991-07-01 00:00:00

  • Effectiveness of multiple initiatives to reduce blood component wastage.

    abstract:OBJECTIVES:Blood component waste is an important issue at all hospitals. As an initiative of the patient blood management program at a regional health care system, the causes and extent of blood product wastage were identified, and targeted interventions to effect a reduction were implemented. METHODS:Multiple low-cos...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP42WMHSSTPHXI

    authors: Collins RA,Wisniewski MK,Waters JH,Triulzi DJ,Yazer MH

    更新日期:2015-03-01 00:00:00

  • Evaluation of real-time clinical decision support systems for platelet and cryoprecipitate orders.

    abstract:OBJECTIVES:To evaluate cryoprecipitate and platelet ordering practices after the implementation of real-time clinical decision support systems (CDSSs) in a computerized physician order entry (CPOE) system. METHODS:Uniform platelet and cryoprecipitate transfusion thresholds were implemented at 11 hospitals in a regiona...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,多中心研究

    doi:10.1309/AJCP1OSRTPUJE9XS

    authors: Collins RA,Triulzi DJ,Waters JH,Reddy V,Yazer MH

    更新日期:2014-01-01 00:00:00

  • A clinician's workstation for improving laboratory use. Integrated display of laboratory results.

    abstract::Physicians are often forced to make decisions about the use of laboratory resources without adequate access to earlier results and related supporting information. Less than optimal use of the laboratory may result. The authors developed and deployed a clinical workstation meant to provide ready access to laboratory in...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/104.3.243

    authors: Connelly DP,Sielaff BH,Willard KE

    更新日期:1995-09-01 00:00:00

  • Cytologic features of poorly differentiated 'insular' carcinoma of the thyroid, as revealed by fine-needle aspiration biopsy.

    abstract::The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.6.687

    authors: Pietribiasi F,Sapino A,Papotti M,Bussolati G

    更新日期:1990-12-01 00:00:00

  • Comparison of two commercially available selective media to screen for vancomycin-resistant enterococci.

    abstract::Campylobacter medium (CAMPY, Becton Dickinson [BD] Microbiology Systems, Cockeysville, MD) and Vancomycin Screen Agar (VSA, BD) were compared for their ability to screen for vancomycin-resistant Enterococcus (VRE) from primary plates. A microdilution minimal inhibitory concentration (MIC) assay (Pasco, BD) served as t...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/TWY5-04QE-9KV0-52MT

    authors: Shigei J,Tan G,Shiao A,de la Maza LM,Peterson EM

    更新日期:2002-01-01 00:00:00

  • Role of flow cytometry in pediatric hematopathology.

    abstract::Multiparameter flow cytometry with optimally selected antibody combinations has expanded the use of this technique beyond traditional applications in hematopathology. By analyzing qualitative patterns of antigen expression on discrete populations or "clusters," one can detect immunophenotypic aberrancy in specific cel...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/1GLC0VY081X8HY2V

    authors: Kroft SH

    更新日期:2004-12-01 00:00:00

  • Solar elastosis in cutaneous melanoma.

    abstract::By studying more than 1,200 patients with cutaneous melanoma and long-term follow-up, I examined the relationship between solar elastosis and age at diagnosis of melanoma, key features of melanoma, and the outcome of overall survival in melanoma. I found that melanomas with elastosis were diagnosed significantly later...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/7MHX96XH3DTY32TQ

    authors: Vollmer RT

    更新日期:2007-08-01 00:00:00

  • Automated immunohistochemical assay for estrogen receptor status in breast cancer using monoclonal antibody CC4-5 on the Ventana ES.

    abstract::Determination of breast cancer estrogen receptor (ER) status as a predictor of tumor response to adjuvant endocrine therapy remains a mainstay of breast cancer management. Recent second generation anti-ER antibodies and new epitope retrieval methods have produced paraffin-based immunohistochemical results that correla...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.3.332

    authors: Nichols GE,Frierson HF Jr,Boyd JC,Hanigan MH

    更新日期:1996-09-01 00:00:00

  • Sarcomatoid carcinoma (so-called pseudosarcoma) of the larynx simulating malignant giant cell tumor of soft parts. A case report.

    abstract::A tumor of the larynx composed of a small infiltrating and well-differentiated squamous cell carcinoma and a large polypoid sarcomatous mass showing the histologic features of the malignant giant cell tumor of soft parts (MGCT) is reported. Other types of sarcomatous components have been described with squamous cell c...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/82.3.340

    authors: Alguacil-Garcia A,Alonso A,Pettigrew NM

    更新日期:1984-09-01 00:00:00

  • Expression of messenger RNA of the cardiac isoforms of troponin T and I in myopathic skeletal muscle.

    abstract::In the absence of clinical signs, elevated values of the cardiac isoforms of troponin T (cTnT) and I (cTnI) can be found in the serum samples of some patients with skeletal muscle myopathies; the cause is unclear. We studied the messenger RNA (mRNA) expression of cTnT and cTnI in the skeletal muscles of 24 patients wi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Messner B,Baum H,Fischer P,Quasthoff S,Neumeier D

    更新日期:2000-10-01 00:00:00

  • Heterogenous expression of endothelial cell markers in infantile hemangioendothelioma. Immunohistochemical study of two solitary cases and one multiple one.

    abstract::Three cases of infantile hemangioendothelioma were immunohistochemically studied with the use of antibodies against von Willebrand factor (vWF), Ulex europaeus I lectin (UEA I), vimentin, thrombomodulin (TM), and actin, as endothelial cell (EC) markers. Because of a broad variety of histologic features, the growth pat...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/91.6.673

    authors: Yasunaga C,Sueishi K,Ohgami H,Suita S,Kawanami T

    更新日期:1989-06-01 00:00:00

  • Cystic parathyroid gland hyperplasia--hyperparathyroidism presenting as a neck mass.

    abstract::Hyperparathyroidism may occasionally present as a neck mass resulting from cystic change in a parathyroid adenoma. We report an unusual instance of hyperparathyroidism presenting as a palpable, but asymptomatic hyperplastic parathyroid gland cyst. We conclude that hyperplastic parathyroid glands may undergo cystic deg...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/77.1.104

    authors: Fallon MD,Haines JW,Teitelbaum SL

    更新日期:1982-01-01 00:00:00

  • A diagnostic expert system for colonic lesions.

    abstract::The diagnostic expert system for colonic lesions (DESCL) was designed to discriminate colonic adenoma and adenocarcinoma from normal colonic tissue. Although it was originally developed for use in conjunction with a machine vision analytic system, the DESCL has evolved into a teaching tool and a model for conceptual m...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: Graham AR,Paplanus SH,Bartels PH

    更新日期:1990-10-01 00:00:00

  • The diagnostic utility of the minimal carcinoma triple stain in breast carcinomas.

    abstract::Pathologists are expected to accurately diagnose increasingly smaller breast carcinomas. Correct classification (ie, lobular vs ductal or in situ vs invasive) directly affects subsequent management, especially when the focus is near a surgical margin or present in a needle core biopsy and is further challenging if the...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPF4O0ADKFMGRJ

    authors: Ross DS,Liu YF,Pipa J,Shin SJ

    更新日期:2013-01-01 00:00:00

  • Comparison of eight kits for the diagnosis of pregnancy.

    abstract::The results obtained with eight pregnancy kits on 735 urines were compared. Overall accuracies were similar and fairly high (93.4 to 95.37%), although only 78.28% of all pregnancies were simultaneously detected by all eight kits. It is suggested that the different performances of each kit could be accounted for not on...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/64.4.452

    authors: Porres JM,D'Ambra C,Lord D,Garrity F

    更新日期:1975-10-01 00:00:00

  • Application of a rapid assay for detection of antibodies to human immunodeficiency virus in urine.

    abstract::The use of rapid, simple tests to detect antibodies to human immunodeficiency virus (HIV) in urine could be valuable for several testing situations, such as in private offices, for epidemiologic surveys, and in developing countries. The authors evaluated the performance of the SUDS HIV type 1 test to detect antibody t...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.2.157

    authors: Constantine NT,Zhang X,Li L,Bansal J,Hyams KC,Smialek JE

    更新日期:1994-02-01 00:00:00

  • Expression of transketolase-like 1 and activation of Akt in grade IV glioblastomas compared with grades II and III astrocytic gliomas.

    abstract::Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/6H9844AMMET82DBJ

    authors: Völker HU,Hagemann C,Coy J,Wittig R,Sommer S,Stojic J,Haubitz I,Vince GH,Kämmerer U,Monoranu CM

    更新日期:2008-07-01 00:00:00

  • Spectrophotometric detection of RT-PCR-amplified BCR/ABL fusion transcripts. A survey performed on archival hematologic slides.

    abstract::We describe a method of spectrophotometric detection of BCR/ABL chimeric sequences amplified by multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), enabling the use of archival hematologic slides as RNA sources. Multiplex PCR amplified b3a2, b2a2, and e1a2 break points of the BCR/ABL translocation and ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/108.4.383

    authors: Attadia V,Alosi M,Improta S,Zagonel V,Martinazzo G,Carbone A,Pinto A

    更新日期:1997-10-01 00:00:00

  • Candidal infection of gastric ulcers. Histology, incidence, and clinical significance.

    abstract::Fungal organisms morphologically resembling Candida were found in one third of 72 consecutive surgically resected gastric ulcers. In over half of these cases the organisms were present in the mycelial form. Large numbers of fungi forming clusters were found in 13 cases (18%) and were associated with a surprisingly hig...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/71.2.137

    authors: Katzenstein AL,Maksem J

    更新日期:1979-02-01 00:00:00

  • Lymph node interdigitating reticulum cell sarcoma.

    abstract::A 67-year-old man presented with a malignant tumor involving abdominal lymph nodes, spleen, liver, and lungs, with associated protracted fever and night sweats. The tumor consisted of large pleomorphic cells, often surrounding microabscesses. Intracytoplasmic S100 protein, surface T6, Leu-3a (T4), and HLA-DR antigens ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/85.6.739

    authors: Chan WC,Zaatari G

    更新日期:1986-06-01 00:00:00

  • Flow cytometric measurement of antiplatelet antibodies.

    abstract::A flow cytometric technic was developed to detect platelet surface-bound immunoglobulin in patients with thrombocytopenia. Elevated platelet surface IgG and/or IgM was detected in 90.9% of patients with immune thrombocytopenia purpura (ITP). False positive results occurred in 9.3% of patients with nonimmune thrombocyt...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/87.4.518

    authors: Rosenfeld CS,Nichols G,Bodensteiner DC

    更新日期:1987-04-01 00:00:00

  • In vitro detection of mild inhibitors to factor VIII in hemophilia.

    abstract::Plasma samples from patients with severe classic hemophilia, suspected of having mild inhibitors not detected in the standard Bethesda inhibitor test, were examined in modified Bethesda tests and in a screening test based on the activated partial thromboplastin time (APTT). The APTT test was more sensitive to mild inh...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/77.6.749

    authors: Ewing NP,Kasper CK

    更新日期:1982-06-01 00:00:00

  • Sensitivity and specificity of antibodies on necrotic tumor tissue.

    abstract::Immunohistochemistry occasionally is used to determine the lineage of entirely necrotic tumors. However, the sensitivity and specificity of antibodies on necrotic tissue are unknown. To determine the usefulness of immunohistochemistry with necrotic lesions, a series of 24 known tumors consisting of 14 carcinomas, 2 ly...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/110.5.641

    authors: Judkins AR,Montone KT,LiVolsi VA,van de Rijn M

    更新日期:1998-11-01 00:00:00

  • Radioimmunoassays for protein C and factor X. Plasma antigen levels in abnormal hemostatic states.

    abstract::Specific radioimmunoassays, sensitive to plasma levels of less than 1% of normal, were developed for protein C and Factor X. In 31 normal subjects, mean plasma antigen levels were as follows: protein C, 3.23 +/- 0.79 microgram/mL (2 SD); Factor X, 7.74 +/- 1.81 microgram/mL. In patient son chronic warfarin therapy, pr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/82.5.573

    authors: Epstein DJ,Bergum PW,Bajaj SP,Rapaport SI

    更新日期:1984-11-01 00:00:00

  • Clinical and economic performance criteria for intraarterial and extraarterial blood gas monitors, with comparison with in vitro testing.

    abstract::The frequency with which blood gas measurements should be obtained is controversial because laboratory-based blood gas analyzers impose blood loss, delay, and cost on each measurement. Point-of-care analyzers eliminate the delay factor, but blood loss and cost factors remain. Optode microsensing can be adopted readily...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: Shapiro BA

    更新日期:1995-10-01 00:00:00

  • Computer-assisted diagnostic surgical pathology reporting.

    abstract::A very simple method of computer-assisted reporting of surgical pathology (including gross description, microscopic description, and diagnosis) has been developed. This method is based on a modification of the existing microbiology program and files in the comprehensive MedLab Company Pathlab computer system, with the...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.3.337

    authors: Davey PW,Vaughan KD

    更新日期:1982-09-01 00:00:00

  • Sporadic medullary carcinoma of the colon: a clinicopathologic comparison with nonhereditary poorly differentiated enteric-type adenocarcinoma and neuroendocrine colorectal carcinoma.

    abstract::We studied 68 sporadic colorectal carcinomas (CRCs) with medullary features (MCRCs) and compared them with 35 poorly differentiated purely "enteric" CRCs (ECRCs) and 15 purely neuroendocrine carcinomas (NECs) of grades II and III, all in patients lacking a family history of CRC. Potential clinicopathologic differences...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Wick MR,Vitsky JL,Ritter JH,Swanson PE,Mills SE

    更新日期:2005-01-01 00:00:00

  • Profiles of black and Latin-American patients having pernicious anemia. HLA antigens, lymphocytotoxic antibody, anti-parietal cell antibody serum gastrin levels, and ABO blood groups.

    abstract::Because of the unusual presentation of pernicious anemia in many black women, a further characterization of this disease among the different racial groups was undertaken. The following were found. (1) Black women under the age of 50 years had a significantly lower prevalence of anti-parietal cell antibody than did "Eu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.3.291

    authors: Carmel R,Ozturk G,Johnson CS,Tung KS,Terasaki PI

    更新日期:1981-03-01 00:00:00