Abstract:
:High affinity binding sites of angiotensin II (A II) have been characterized in rabbit renomedullary interstitial cells in tissue culture. Binding was rapid, specific, and saturable. Scatchard analysis of steady state saturation binding data at 22 degrees C indicated a single binding site with an equilibrium dissociation constant of 3.1 mM. The binding inhibition activities of analogues of angiotensin II correlated with their biological potencies: [Sar1, Ala8]A II greater than [des-Asp1]A II greater than greater than 3--8 hexapeptide. The biologically inactive 1--7 heptapeptide failed to displace the labeled angiotensin II from its binding sites. Angiotensin II directly stimulated prostaglandin E2 biosynthesis by the rabbit renomedullary interstitial cells in tissue culture. The concentration of angiotensin II that resulted in half maximal stimulation of prostaglandin E2 biosynthesis (6.5 nM) correlated with the concentration of angiotensin II that resulted in half maximal occupancy of binding sites (3.1 nM). The relative potency of [des-Asp1] A II as a stimulus for prostaglandin E2 biosynthesis was proportional to its relative binding inhibition activity. These findings demonstrate the biological significance of the angiotensin II binding sites in the rabbit renomedullary interstitial cells in tissue culture and suggest that the cells may provide a homogeneous model tissue for the study of the angiotensin receptor.
journal_name
Circ Resjournal_title
Circulation researchauthors
Brown CA,Zusman RM,Haber Edoi
10.1161/01.res.46.6.802subject
Has Abstractpub_date
1980-06-01 00:00:00pages
802-7issue
6eissn
0009-7330issn
1524-4571journal_volume
46pub_type
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journal_title:Circulation research
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.113.301896
更新日期:2013-11-08 00:00:00
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更新日期:2004-06-11 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.67.2.415
更新日期:1990-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.66.5.1381
更新日期:1990-05-01 00:00:00
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更新日期:1979-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-07-06 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.52.6.730
更新日期:1983-06-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.45.3.404
更新日期:1979-09-01 00:00:00
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更新日期:2020-02-28 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.69.4.1022
更新日期:1991-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2011-10-14 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2016-06-24 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2004-11-26 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2003-03-21 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000200739.90811.9f
更新日期:2006-02-03 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.108.180869
更新日期:2008-10-10 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.59.5.529
更新日期:1986-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.76.5.832
更新日期:1995-05-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2001-08-17 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.66.6.1755
更新日期:1990-06-01 00:00:00
abstract:RATIONALE:The sarcolemma of cardiomyocytes contains many proteins that are essential for electromechanical function in general, and excitation-contraction coupling in particular. The distribution of these proteins is nonuniform between the bulk sarcolemmal surface and membrane invaginations known as transverse tubules ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.120.317266
更新日期:2021-01-22 00:00:00
abstract::Five fetal sheep (gestational age, 118 to 125 days) were instrumented to measure impedances of the lower abdominal aorta. Four days after surgery, flow and pressure pulses were recorded during control conditions and during infusion of norepinephrine (0.3 to 3 micrograms.min-1.kg-1 body weight) or angiotensin II (0.2 t...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.74.4.641
更新日期:1994-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.82.10.1078
更新日期:1998-06-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.76.6.987
更新日期:1995-06-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2004-05-28 00:00:00
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journal_title:Circulation research
pub_type: 评论,杂志文章
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更新日期:2009-06-05 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.0000052313.23087.3f
更新日期:2003-02-07 00:00:00
abstract::G-protein receptor kinase 2 (GRK2) is 1 of 7 mammalian GRKs that phosphorylate ligand-bound 7-transmembrane receptors, causing receptor uncoupling from G proteins and potentially activating non-G-protein signaling pathways. GRK2 is unique among members of the GRK family in that its genetic ablation causes embryonic le...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000247932.71270.2c
更新日期:2006-10-27 00:00:00