Abstract:
:Human K cells are able to lyse human lymphoblastoid B cells in the presence of specific anti-human immunoglobulin isotype antibodies. The human lymphoblastoid B-cell line DAUDI (surface mu- and kappa-chains positive) was lysed in the presence of anti-mu and anti-kappa antisera, or IgG fraction of these antisera, and B-cell-depleted human lymphocytes. Lysis was not induced by anti-isotypic antisera alone or human lymphocytes alone. Lysis was not induced by antisera directed at isotypes which were not expressed on the DAUDI cells. The human lymphoblastoid B-cell line RAJI, which does not express surface membrane isotypes, could not be lysed by the anti-isotype-dependent cell-mediated mechanism. Lysis of DAUDI cells by this mechanism was mediated by Fc gamma receptor-bearing human non-B lymphocytes and required an intact Fc piece in the inducing anti-isotypic antibody. These observations are discussed as a possible role for K cells in the regulation of immune responses by interaction between anti-idiotypic antibodies and idiotype-bearing cells.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Caraux J,Weigle WOdoi
10.1016/0008-8749(83)90380-5subject
Has Abstractpub_date
1983-03-01 00:00:00pages
372-8issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(83)90380-5journal_volume
76pub_type
杂志文章abstract::The lineage of lymphokine-activated killer (LAK) cells is poorly understood. To examine the relationship between LAK and natural killer (NK) cells we utilized two congenitally immunodeficient mice, namely severe combined immunodeficient (scid) and athymic (nude) mice that lack T cells but have normal NK cells. LAK act...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90190-1
更新日期:1989-04-15 00:00:00
abstract::Burn injury induces immune dysfunction and alters numerous physiological parameters. While clinical studies indicate that burn injury size profoundly impacts patient immune status, only limited experimental studies have systematically addressed its impact on immune functional parameters. In the present study, mice wer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00024-8
更新日期:2002-11-01 00:00:00
abstract::Human mononuclear cells stimulated with soluble IgG aggregates generated chemiluminescence, a response attributable to monocytes. Some requirements of this reaction were examined by preincubation of the cells with a variety of inhibitors. The protease antagonists TPCK and TLCK, the phospholipase inhibitors quinacrine ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90149-7
更新日期:1984-08-01 00:00:00
abstract::T-Cell subsets identified by polyclonal and monoclonal antibodies to dipeptidyl peptidase IV (DP IV) were investigated. Analysis in a cytofluorograf revealed 63 +/- 7% positive scatter-gated T lymphocytes. DP IV-positive cells were found to be T11+, 74-81% OKT4+, and 12-19% OKT8+. DP IV-negative cells were T11+ and co...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90400-9
更新日期:1985-06-01 00:00:00
abstract::We have previously demonstrated that natural killer (NK) cells are lysosome-rich and stain more intensely with lysosomotropic agents such as neutral red and quinacrine (Qu) than do non-NK cells. In this study we combined the quantitation of Qu staining with surface marker staining to define subpopulations of NK cells....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90158-x
更新日期:1988-08-01 00:00:00
abstract::In this study, the major histocompatibility complex-unrestricted cytotoxic effectors elicited in human peripheral blood mononuclear cells (PBMC) by a mannoprotein (MP) component from the cell wall of the human indigenous microorganism Candida albicans have been compared with those obtained by stimulation with interleu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90033-6
更新日期:1989-07-01 00:00:00
abstract::A recombinant form of human soluble CD23 (sCD23), the low affinity receptor for IgE (FcepsilonRII), was produced by PCR cloning the lectin-binding domain sequence into a bacterial expression vector. After renaturation and purification, the sCD23 bound IgE and divalent metal ions, indicating its activity. The recombina...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.06.006
更新日期:2005-04-01 00:00:00
abstract::The mechanism of polyclonal B cell activation in autoimmune diseases was investigated by using an autoreactive B cell clone established by somatic hybridization with B cells derived from NZB X NZW (B/W) F1 mice. Briefly, splenic B cells from B/W F1 mice were fused with M12.4.1, a mutant of a B cell line, in the presen...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90080-9
更新日期:1992-02-01 00:00:00
abstract::Varying the concentration of anti-CD3 immobilized on Sepharose beads allowed us to study both the inhibitory and the stimulatory effects of IL-4 on purified T cells and contrast IL-4 versus IL-2-driven T-cell proliferative responses. In the presence of low density immobilized anti-CD3, IL-4 was unable to stimulate pur...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1081
更新日期:1993-04-01 00:00:00
abstract::Mice that received a sublethal, intraperitoneal dose of viable Listeria monocytogenes, virulent strain 10403, exhibited a systemic increase in natural killer (NK) activity. The kinetics of the response differed with respect to the various effector cell populations analyzed. Resident peritoneal cells and peripheral blo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90108-4
更新日期:1984-04-01 00:00:00
abstract::Mice from strains showing either phenotypical expression of Bcg gene (C57BL/6, BALB/c, DBA/1, and (C57BL/6 X DBA/2)/F1, CBA, A/J, DBA/2) were infected intravenously with 10(7) Mycobacterium lepraemurium (MLM). The number of acid-fast bacilli (AFB) within the spleens, the ability of spleen cells to produce in vitro int...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90026-7
更新日期:1985-03-01 00:00:00
abstract::The purpose of the present study was to compare the phenotype of tissue macrophages with that of their precursors in the bone marrow and blood. The phenotype was determined on the basis of the quantitative binding of monoclonal antibodies to cell-surface antigens (antigen F4/80, complement receptor III, Fc receptor II...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90085-2
更新日期:1987-04-01 00:00:00
abstract::To investigate a role for T lymphocytes in primary demyelination of central nervous system (CNS) tissue, antigen-specific T cell lines sensitized to myelin-associated and myelin-unrelated antigens were developed from SJL mice and tested on myelinated organotypic cultures of syngeneic spinal cord. Demyelination was ass...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90340-0
更新日期:1986-10-01 00:00:00
abstract::The relay hypothesis [R. Nayak, S. Mitra-Kaushik, M.S. Shaila, Perpetuation of immunological memory: a relay hypothesis, Immunology 102 (2001) 387-395] was earlier proposed to explain perpetuation of immunological memory without requiring long lived memory cells or persisting antigen. This hypothesis envisaged cycles ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.05.001
更新日期:2007-03-01 00:00:00
abstract::Bcl-2 proto-oncogene is expressed in normal lymphoid tissues and its role has been suggested to involve the selection of B and T cells. Here we report three-color flow cytometric studies on the expression of bcl-2 protein in the thymus and periphery. Bcl-2 was expressed in the majority of T and B cells and down-regula...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1127
更新日期:1994-05-01 00:00:00
abstract::Altered neutrophil function may contribute to the development of AIDS during the course of HIV infection. It has been described that Nef, a regulatory protein from HIV, can modulate superoxide production in other cells, therefore altered superoxide production in neutrophils from HIV infected patients, could be seconda...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.03.012
更新日期:2010-01-01 00:00:00
abstract::To understand the role of cAMP during macrophage activation, we investigated the effects of various cAMP analogues in the induction of nitric oxide (NO) synthesis in murine peritoneal macrophages. Intracellular cAMP modulators such as N6,O2'-dibutyryl cyclic AMP (DB-cAMP), 8-bromo-cAMP, or 8-chloro-cAMP had no effect ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1144
更新日期:1997-07-10 00:00:00
abstract::The impact of BCR:CD21 co-engagement on B cell expression of molecules critical for T cell activation was investigated with receptor-specific mAbs conjugated to high MW dextran as stimulatory ligands. In the absence of IL-4, BCR:CD21 co-ligation augmented BCR-triggered CD86 only under conditions of very low BCR ligand...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00512-9
更新日期:2002-03-01 00:00:00
abstract::An important subset in regulating antitumor immunity is the maturation and accumulation of intratumor dendritic cells (DCs), inducing potent T cell cytotoxicity. In this study, we explored how the soluble abundant high-mobility group box 1 protein (HMGB1) affected DC activation and retention within lung cancers, and i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.08.011
更新日期:2019-09-01 00:00:00
abstract::A previous report (G. E. Woloschak and D. Senitzer, submitted for publication) has demonstrated that mitogen-stimulated splenocytes fuse at a much higher frequency than untreated splenocytes as measured by the fusion index (a calculation of the number of nuclei in fused cells vs the total number of nuclei). A measurem...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90059-x
更新日期:1983-07-01 00:00:00
abstract::It has been documented that interleukin-6 (IL-6) supports the proliferation of purified, anti-CD3-stimulated murine T cells. We found that stimulation of human peripheral blood mononuclear cells (PBMCs) with anti-CD3 induced a significant accumulation of IL-6 mRNA, indicating that antigen-mediated T-cell activation ma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90322-3
更新日期:1991-05-01 00:00:00
abstract::We have previously described a novel human B cell differentiation factor (BCDF), 446-BCDF, that is distinct biochemically and functionally from other cytokines. Since signal transduction pathways involved in human B cell differentiation have been incompletely studied and are poorly understood, we assessed the effects ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1050
更新日期:1995-04-15 00:00:00
abstract::Mitogenic stimulation of sublines of the human histiocytic lymphoma, U937, results in the production of an inhibitor of lymphocyte proliferation (IDS-U937). The relationship of IDS-U937 to alpha- and gamma-interferon was examined by several techniques: (1) the antiviral activity of the IDS preparations was determined,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90390-3
更新日期:1984-07-01 00:00:00
abstract::The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.103987
更新日期:2020-01-01 00:00:00
abstract::The proliferative responses of human peripheral blood mononuclear cells (PBL) and thymocytes to OKT3 monoclonal antibody have been investigated. The PBL response to OKT3 was maximal after 72 hr while that of thymocytes was inappreciable at all times measured. Unlike phytohemagglutinin, OKT3 was unable to elicit the mi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90105-9
更新日期:1984-04-01 00:00:00
abstract::The role of protein synthesis during the activation of macrophages (M phi) by lymphokines (LK) was studied. Peritoneal murine macrophages elicited by proteose-peptone (pM phi) were activated with LK (supernatants from normal mouse spleen cells pulsed with concanavalin A) and tested for cytotoxicity in an 18 hr assay a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90273-9
更新日期:1984-04-15 00:00:00
abstract::Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca(2+)-dependent activation pathway. In the present experiments we studied the pr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.01.004
更新日期:2013-01-01 00:00:00
abstract::In previous studies, the murine SaI (A/J derived, KkDd) sarcoma was transfected with the allogeneic MHC class I H-2Kb gene, and expressed high levels of H-2Kb antigen. Contrary to expectations, the tumor cells expressing the alloantigen (SKB3.1M tumor cells) were not rejected by autologous A/J mice. Because these resu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90319-7
更新日期:1991-05-01 00:00:00
abstract::Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus transformed B cells is mainly mediated by LFA-1 (CD11a/CD18) and CD2 molecules. Low-affinity binding of resting T cells can be transiently upregulated by cross-linking of CD3-TCR (T cell receptor) complexes. This inside-out signaling influences i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1211
更新日期:1993-09-01 00:00:00
abstract::Evidence is presented that rat serosal mast cells and cells of the rat basophilic leukemia line, RBL-1, each contain a calcium-activated, phospholipid-dependent protein kinase. The enzymes are very similar in their activation requirements to the calcium-dependent enzymes termed protein kinase Cs in brain. The enzyme i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90098-5
更新日期:1986-12-01 00:00:00