Affinity sensor: a new technique for developing implantable sensors for glucose and other metabolites.

Abstract:

:We describe affinity sensors for monitoring various metabolites in blood plasma by optical means. The principle of detection is similar to that used in radioimmunoassays and is based on the competitive binding of a particular metabolite and a fluorescein-labeled analogue with receptor sites specific for the metabolite and the labeled ligand. This concept has been directed toward the development of an affinity sensor for glucose. Concanavalin A, a protein with specific binding character for glucose, was immobilized on the inside surface of a hollow dialysis fiber. Fluorescein-labeled dextran was selected as the competitive labeled ligand. The molecular weight cutoff of the dialysis fiber is low enough to completely retain the 70,000 MW dextran within the fiber lumen while glucose can freely pass through the dialysis membrane. The sensor is completed by inserting a single optical fiber in the lumen of the dialysis fiber, thus allowing measurement of the unbound FITC-dextran. Preliminary tests of the sensor indicated the feasibility of the approach. Sensitivity to glucose in the physiologic range was obtained, but further work will be required to optimize the sensitivity and response time of the sensor.

journal_name

Diabetes Care

journal_title

Diabetes care

authors

Schultz JS,Mansouri S,Goldstein IJ

doi

10.2337/diacare.5.3.245

subject

Has Abstract

pub_date

1982-05-01 00:00:00

pages

245-53

issue

3

eissn

0149-5992

issn

1935-5548

journal_volume

5

pub_type

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