Abstract：
:ATP mediates covalent binding of hydroxymethyl derivatives of aromatic hydrocarbons to DNA. This non-enzymatic reaction has been studied with 6-[14C]hydroxymethylbenzo[alpha]pyrene (]14C]BP-6-CH2OH) and 7-[14C]-hydroxymethylbenz[alpha]anthracene ([14C]BA-7-CH2OH) at 37 degrees C in Tris buffer (pH 7.0). While ADP mediates the reaction 25-50% as well as ATP, six other possible phosphate donors including AMP were inactive as cofactors. A complex response to ATP occurred in which low binding of BP-6-CH2OH or BA-7-CH2OH was observed at concentrations of ATP below 2.5 mM, but a greater than linear response to higher concentrations of ATP was observed until ATP was saturating. Binding of the substrates to RNA was much lower than to DNA. Fluorescence spectra of BP-6-CH2OH bound to DNA were almost identical to the spectra of 6-bromomethylbenzo[alpha]pyrene bound to DNA and free 6-methylbenzo]alpha]pyrene, indicating that ATP-mediated binding of BP-6-CH2OH to DNA occurs at the 6-methyl group. The fate of ATP and ADP in the binding reaction of BP-6-CH2OH was examined by thin layer chromatography. Loss of one phosphate group occurs during the reaction. With ATP the rate of loss is about 100-fold greater than the rate of binding of BP-6-CH2OH to DNA. This implies that the binding reaction proceeds through formation of a presumed reactive and unstable phosphate ester intermediate which then inefficiently binds to DNA.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Rogan EG,Roth RW,Katomski-Beck PA,Laubscher JR,Cavalieri ELdoi
10.1016/0009-2797(80)90138-6subject
Has Abstractpub_date
1980-07-01 00:00:00pages
51-63issue
1eissn
0009-2797issn
1872-7786pii
0009-2797(80)90138-6journal_volume
31pub_type
杂志文章abstract：:Two aldehyde dehydrogenases (ALDH) were purified from the liver of black seabream (Acanthopagrus schlegeli). Chromatography of the liver homogenate on an alpha-cyanocinnamate-Sepharose affinity column results in two activity peaks using acetaldehyde as the substrate. The eluate was subjected to another affinity chroma...
journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章,评审
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/j.cbi.2013.11.006
更新日期：2014-02-25 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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更新日期：2015-07-05 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：1994-10-01 00:00:00
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journal_title：Chemico-biological interactions
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更新日期：2015-02-25 00:00:00
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更新日期：2018-01-05 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章,评审
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更新日期：2010-11-05 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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journal_title：Chemico-biological interactions
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更新日期：2013-12-05 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：2008-09-25 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/0009-2797(79)90030-9
更新日期：1979-08-01 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/s0009-2797(01)00253-8
更新日期：2001-09-28 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/0009-2797(75)90061-7
更新日期：1975-07-01 00:00:00
