Purification of mouse interferon on specifically purified immunoadsorbent.

Abstract:

:A one-step purification procedure using affinity chromatography on purified anti-interferon antibodies makes possible to obtain highly purified interferon preparations. Antibodies against purified mouse fibroblast interferon were covalently bound to activated CNBr-Sepharose 4B. This immunoadsorbent had a binding capacity of 125,800 mouse fibroblast interferon units per 4.8 ml of gel. The binding capacity for mouse leukocytes was similar while for serum interferon it was lower. When analyzed in polyacrylamide gel, the electrophoretic profiles of purified IF had a similar shape to that of unpurified IF. The results of this purification procedure are not influenced by volume, biological activity and/or purity of the starting material.

authors

Hajnicka V,Fuchsberger N,Borecký L

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

619-22

issue

5

eissn

0004-069X

issn

1661-4917

journal_volume

25

pub_type

杂志文章
  • Variant and Specific Forms of Autoimmune Cholestatic Liver Diseases.

    abstract::Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) are the main autoimmune cholestatic liver diseases. IgG4-associated sclerosing cholangitis is another distinct immune-mediated cholestatic disorder of unknown aetiology that is frequently associated with autoimmune pancreatitis or other IgG4-re...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-019-00550-9

    authors: Dalekos GN,Gatselis NK

    更新日期:2019-08-01 00:00:00

  • Immunotherapy as an Option for Cancer Treatment.

    abstract::The progress in melanoma immunotherapy highlights the importance of immunotherapy for cancer treatment. Although the concept of immunotherapy emerged in the beginning of the twentieth century, the end of the century signaled the start of modern immunotherapy, which has recently allowed a staggering progress in the fie...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-017-0491-5

    authors: Rusch T,Bayry J,Werner J,Shevchenko I,Bazhin AV

    更新日期:2018-04-01 00:00:00

  • Enzymatic method of incorporation of 125I into DNA.

    abstract::DNA can be labeled with iodine in the reaction catalyzed with lactoperoxidase. Iodine is incorporated in cytosine similarly as in the chemical reaction. Iodination by means of the enzymatic method is faster than by means of the chemical method. The equilibrium state is still achieved after 10 minutes, during the enzym...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Gładek A,Mordarski M

    更新日期:1983-01-01 00:00:00

  • Long-term assessment of interferon responses in the HIV+ and AIDS patients.

    abstract::Impairment of interferon (IFN) system in human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS) became a basis for searching IFN responses to monitor the disease progression. For detailed investigations 16 HIV+/AIDS patients with at least 4 successively taken blood samples available...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Piasecki E,Knysz B,Inglot M,Simon K,Gładysz A

    更新日期:1996-01-01 00:00:00

  • Surface markers on human activated T lymphocytes. II. CD4 and CD8 differentiation antigens.

    abstract::In order to estimate the dependence of CD4 and CD8 antigen expression upon the cell activation, their presence on resting and activated human T cells was investigated. Peripheral blood lymphocytes of healthy donors derived from late (TEl) and early (TEe) E rosettes were used as resting and presumably in vivo activated...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Kontny E

    更新日期:1989-01-01 00:00:00

  • Antigen-restricted gammadelta T-cell receptors?

    abstract::After more than two decades of investigation, the biological role of the gammadelta T-cell receptors (TCRs) remains elusive. In fact, a theory of ligand recognition is still lacking that accounts for their adaptable structure, their peripheral selection, and the observed responses of gammadelta T cells, which do not r...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-009-0017-x

    authors: Born WK,O'Brien RL

    更新日期:2009-03-01 00:00:00

  • The immunological effects of interleukin 2 therapy in HIV+ patients.

    abstract::Human immunodeficiency virus (HIV) infection produces a profound impairment of immune functions that antiretroviral therapy is unable to restore. Because of its immuno-enhancing properties, interleukin 2 (IL-2) has been used as a therapeutic tool in HIV+ subjects. IL-2 produces an increase of CD4 and CD8 lymphocyte ab...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: De Paoli P

    更新日期:2000-01-01 00:00:00

  • Pharmacological properties of racemic chlorobromofosfamide.

    abstract::Racemic chlorobromofosfamide (CBM-4A) is a bromine substituted analog of ifosfamide. The studies reported here were aimed at establishing the general pharmacological properties and toxicologic profile of this compound. Acute and cumulative toxicity, behavioral screening tests and effects of subchronic and chronic trea...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Juszkiewicz M,Kleinrok Z,Sawiniec Z

    更新日期:1994-01-01 00:00:00

  • Studies on the human mixed lymphocyte culture. I. The influence of the irradiated autogenic and allogenic lymphocytes on DNA synthesis.

    abstract::Fourteen patients with chronic renal failure and their prospective living donors were examined. DNA uptake by donors' monocultures, recipients' monocultures and mixed cultures was measured. The influence of X-ray treated cells on proliferative capacity of untreated cells in these three types of cultures was studied. T...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Wojtulewicz-Kurkus J,Skopińska E,Nowaczyk M

    更新日期:1978-01-01 00:00:00

  • Acute Thymic Involution and Mechanisms for Recovery.

    abstract::Acute thymic involution (ATI) is usually regarded as a virulence trait. It is caused by several infectious agents (bacteria, viruses, parasites, fungi) and other factors, including stress, pregnancy, malnutrition and chemotherapy. However, the complex mechanisms that operate during ATI differ substantially from each o...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-017-0462-x

    authors: Ansari AR,Liu H

    更新日期:2017-10-01 00:00:00

  • Positive and negative regulatory mechanisms in high-affinity IgE receptor-mediated mast cell activation.

    abstract::Mast cells are important effector cells in allergic inflammatory reactions. The aggregation of the high-affinity IgE receptor (FcepsilonRI) on the surface of mast cells initiates a complex cascade of signaling events that ultimately leads to the release of various mediators involved in allergic inflammation and anaphy...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-008-0041-2

    authors: Roth K,Chen WM,Lin TJ

    更新日期:2008-11-01 00:00:00

  • Anti-Pseudomonas immunoglobulin I. Immunization of sheep.

    abstract::The production of sheep Pseudomonas immune serum is described. The animals were immunized with Pseudomonas vaccine prepared from strains of Pseudomonas aeruginosa belonging to seven immunotypes of Fisher's schema. Immune sera obtained from hyperimmunized sheep are characterized by a high level of stabel antibodies wit...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Schiller B,Buchowicz I,Korbecki M,Lysakowska E,Rudnicka W,Robaczewska E

    更新日期:1981-01-01 00:00:00

  • Potential for immune reconstitution through G-CSF treatment of HIV patients.

    abstract::New treatment strategies for HIV/AIDS are very successful in reducing viral load. However, reconstitution of the immune system takes about one year and may be insufficient or remain incomplete. During this time the patient remains prone to opportunistic infections as a result of the complex immune dysfunction caused b...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:

    authors: Von Aulock S,Hartung T

    更新日期:2002-01-01 00:00:00

  • The physiological role of regulatory T cells in the prevention of autoimmunity: generation, specificity and mode of action.

    abstract::Until recently, the traditional view was that tolerance to self antigens was maintained by a combination of physical deletion, in the thymus, and functional deletion, in the periphery, of autoreactive T cells. There is now, however, abundant evidence that the normal T cell repertoire contains overtly autoreactive T ce...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:

    authors: Seddon B

    更新日期:2000-01-01 00:00:00

  • Proliferation of human lymphocytes by mitogen stimulated cells.

    abstract::Lymphocytes cultured for three days with PHA or Con A were capable of inducing vigorous proliferation of autologous or allogeneic lymphocytes. Kinetics of the response varied with mitogen dose used for obtaining stimulating cells and with the ratio of stimulating to responder cells. A requirement for viable, mitogen-r...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Jaroszewski J,Kurpisz M,Brelińska-Peczalska R,Lastowska M

    更新日期:1978-01-01 00:00:00

  • Antigenic characterization of urea-extractable crude burn toxin.

    abstract::Crude burn toxin was obtained from human skin scalded for 1 min at 90 degrees C in vitro. The toxin solubilized with 8 M urea was administered to rabbits to obtain antiserum. The antigenic analysis carried out by immunodiffusion and immunoelectrophoresis demonstrated that urea-extractable toxin solution differed from ...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Grzybowski J,Bogusławska-Kubin J

    更新日期:1981-01-01 00:00:00

  • Distinct VEGF functions during bone development and homeostasis.

    abstract::Vascular endothelial growth factor-A (VEGF) is a key regulator of physiological hemangiogenesis during development, postnatal growth, and homeostasis. It is well known that VEGF is required for effective coupling of angiogenesis to endochondral and membranous bone formation during skeletal development. However, less w...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-014-0285-y

    authors: Liu Y,Olsen BR

    更新日期:2014-10-01 00:00:00

  • Interleukin 6, tumor necrosis factor alpha and their soluble receptors in the blood serum of patients with denture stomatitis and fungal infection.

    abstract::Determinations of the blood serum levels of interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and their soluble receptors (sIL-6R, sTNFR) in denture stomatitis patients (DS) were performed. Serum levels of interleukins and their soluble receptors were measured using the ELISA method. In all examined patien...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Pietruski JK,Pietruska MD,Jabłońska E,Sacha P,Zaremba M,Stokowska W

    更新日期:2000-01-01 00:00:00

  • BH3-only proteins: the lords of death.

    abstract::Although the mechanisms by which Bcl-2 family proteins control the apoptotic machinery of the cell are not fully understood, it becomes clear that the role of BH3-only proteins consists in serving as sensors or sentinels of cellular damage, transducing the apoptotic stimuli to the mitochondria. For this reason, mammal...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:

    authors: Fleischer A,Rebollo A,Ayllón V

    更新日期:2003-01-01 00:00:00

  • Effect of immunosuppressive agents on the course of experimental syphilis in rabbits.

    abstract::Rabbits infected intradermally with T. pallidum at four sites on the clipped back were treated with cyclophosphamide, 1 mg/kg body weight, twice before and every two days after inoculation for five months, or 5-fluorouracil 2.5 mg/kg body weight, according to the same regimen. Humoral response, as measured repeatedly ...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Pacha J,Metzger M,Smogór W,Michalska E,Podwińska J,Ruczkowska J

    更新日期:1979-01-01 00:00:00

  • Mouse monoclonal IgA antibodies lack interchain disulfide bonds.

    abstract::A mouse monoclonal IgA antibody (A008), anti-blood group A antigen, was shown to dissociate into heavy (H) and light (L) polypeptide chains under non-reducing conditions. The dissociation occurred in the presence of 0.2% sodium dodecyl sulphate (2 h at room temperature) or at elevated temperature (1 h at 70 degrees C)...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Krotkiewski H,Laskowska A,Krotkiewska B

    更新日期:1995-01-01 00:00:00

  • Pharmacological studies on new oncostatic acridine derivatives. I. Acute and subchronic action.

    abstract::Preclinical pharmacological studies of two acridine derivatives, dihydrochloride N10-oxide 1-nitro-9-/3-dimethylaminopropylamino/-acridine (C-666) and dihydrochloride 1-nitro-9-[(2-dimethylamino)-1-methylethylamino]-acridine (C-829) are reported. Both compounds are characterized by biological activity, poor absorption...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Błaszczyk B,Giełdanowski J,Patkowski J,Kowalczyk-Bronisz SH

    更新日期:1979-01-01 00:00:00

  • HLA and bone marrow transplantation.

    abstract::Bone marrow transplantation between HLA-identical siblings is an established therapeutic strategy in leukemia and a variety of lympho-hematopoietic disorders. In recent years unrelated donors were increasingly used for patients lacking a family donor. Here we report on current progress in HLA class II typing by molecu...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:

    authors: Eiermann TH

    更新日期:1995-01-01 00:00:00

  • Pharmacological activity of 3-(5-barbiturylo)-propanesulfonic acids derivatives.

    abstract::Pharmacological activity of 6 newly synthetized water-soluble derivatives of barbiturylo propanosulfonic acids, was evaluated. The compounds were obtained under the conditions of the Ritter reaction. The experiments were performed with the aim to determine the anti-inflammatory and immunotropic activities as well as t...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Kowalczyk-Bronisz SH

    更新日期:1984-01-01 00:00:00

  • Evaluation of Endothelial Function by Flow-Mediated Dilation: a Comprehensive Review in Rheumatic Disease.

    abstract::Flow-mediated dilation (FMD) represents a non-invasive marker of endothelial function to evaluate vascular homeostasis, which reflects the effects of several mechanisms, including vessel tone regulation, cell proliferation, and inflammatory responses. Beyond classical atherosclerotic risk factors such as arterial hype...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-017-0465-7

    authors: Moroni L,Selmi C,Angelini C,Meroni PL

    更新日期:2017-12-01 00:00:00

  • Immunologic phenomena in experimental rodentiosis. II. Cellular and humoral factors in immunity to infection with Yersinia pseudotuberculosis.

    abstract::Humoral and cellular mechanisms of immunity to infection with Y. pseudotuberculosis bacilli in mice were studied. Development of delayed hypersensitivity, measured by the footpad test and macrophage migration inhibition test, was significantly correlated with immunity, expressed by elimination of Y. pseudotuberculosis...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Jakoniuk P

    更新日期:1976-01-01 00:00:00

  • T lymphocytes modifying factors in sera of patients with Myasthenia gravis.

    abstract::The examination of patients with Myasthenia gravis revealed that before thymectomy the number of T and B cells in the peripheral blood did not deviate from the norm. It was also observed that their sera contained factor stimulating T lymphocytes to the spontaneous rosette formation, theta antigen generation in xynogen...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Skibiński G,Ujma B,Guzowski K,Brzecki A,Zwoliński J,Wieczorek Z

    更新日期:1983-01-01 00:00:00

  • The effect of Chlamydia trachomatis infection of the prostate gland on the concentration of citric acid.

    abstract:INTRODUCTION:The objective of the study was to assess the relationship between Chlamydia trachomatis (C.t.) infection of the prostate and the concentration of citric acid. MATERIALS AND METHODS:The study involved 60 patients with chronic prostatitis (NIH III). Urethral swabs and expressed prostatic secretions (EPS) we...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:10.1007/s00005-006-0009-z

    authors: Zdrodowska-Stefanow B,Ostaszewska-Puchalska I,Badyda J,Galewska Z

    更新日期:2006-01-01 00:00:00

  • A rabbit used as a model for testing hematopoietic tissue transplants.

    abstract::Allogenic transplantations of bone marrow or spleen cells were carried out on rabbits treated with cyclophosphamide. About 50% of the recipients developed chimerism of peripheral blood cells. Chimerism was a passing phenomenon prevailing from 14 to 180 days after grafting. A phenomenon indicating that not all cell lin...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章

    doi:

    authors: Kosiński S,Matej H

    更新日期:1978-01-01 00:00:00

  • Potential immunological functions of salivary Hsp70 in mucosal and periodontal defense mechanisms.

    abstract::Molecular chaperones were considered to be intracellular, but there is increasing evidence demonstrating their cytoprotective and immune modulator properties outside the cell. The major extracellular chaperone (Hsp70) was also found in saliva, indicating a possible effect of Hsp70 on mucosal surfaces. Here we summariz...

    journal_title:Archivum immunologiae et therapiae experimentalis

    pub_type: 杂志文章,评审

    doi:10.1007/s00005-007-0012-z

    authors: Fábián TK,Fejérdy P,Nguyen MT,Soti C,Csermely P

    更新日期:2007-03-01 00:00:00