Abstract:
:The abundance of membrane cholesterol is an important determinant of the functional properties of biomembranes. To determine whether arterial smooth muscle acquires altered contractile properties in a high cholesterol environment, isolated canine coronary arteries were exposed to cholesterol in stable aqueous solution. Cholesterol, 10(-12) to 10(-10) M, was an efficacious vasoconstrictor, as maximum contractions equaled those obtained with 15 mM KCl. Antiadrenergic interventions, including chemical sympathectomy in vivo with 6-hydroxydopamine and alpha- and beta-adrenergic blockade with phentolamine and L-propranolol (both 10(-6 M), did not significantly attenuate the contractions. However, responses to cholesterol were abolished completely by (+/-)-verapamil (10(-6) M). Cholesterol in picomolar concentration enhanced the constrictor effects of CaCl2 and KCl, both in the presence and absence of alpha- and beta-adrenergic blockade. Increases in tone in response to graded elevations in the CaCl2 concentration (0-2 mM) were augmented up to 1.5-fold by 10(-12) M cholesterol (P less than 0.01). Results indicate that cholesterol sensitizes isolated coronary arteries to external Ca2+ by a nonadrenergic mechanism. The findings are consistent with the hypothesis that acquisition of membrane cholesterol may alter the contractile properties of coronary arterial smooth muscle, a phenomenon that could play a role in the pathophysiology of atherosclerotic heart disease.
journal_name
Circ Resjournal_title
Circulation researchauthors
Yokoyama M,Henry PDdoi
10.1161/01.res.45.4.479subject
Has Abstractpub_date
1979-10-01 00:00:00pages
479-86issue
4eissn
0009-7330issn
1524-4571journal_volume
45pub_type
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更新日期:1984-11-01 00:00:00
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更新日期:1977-04-01 00:00:00
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更新日期:1987-04-01 00:00:00
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更新日期:1994-05-01 00:00:00
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更新日期:1995-02-01 00:00:00
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更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-07-09 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.65.6.1735
更新日期:1989-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-02-03 00:00:00
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更新日期:1999-02-19 00:00:00
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更新日期:2000-09-29 00:00:00
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更新日期:1982-02-01 00:00:00
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更新日期:2014-01-17 00:00:00
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更新日期:1982-11-01 00:00:00
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更新日期:1981-04-01 00:00:00
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更新日期:1994-10-01 00:00:00
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更新日期:1997-06-01 00:00:00