Clinical application of cytochrome a(plus a3) assay of mitochondria from liver specimens: an aid in determining metabolic tolerance of liver remnant for hepatic resection.

Abstract:

:Measurement of cytochrome a(+a(3)) contents in liver mitochondria was made on 52 biopsy specimens of patients with liver tumor. Patients having higher cytochrome a(+a(3)) contents in mitochondria from remaining liver than those of normal human liver mitochondria could survive well major liver resections whether or not associated with liver cirrhosis. However, patients with cytochrome a(+a(3)) contents less than 0.5 x 10(-10) moles per mg protein showed a high rate of postoperative complication (80%) and death (40%) in spite of minor operation. In routinely used liver function tests such as serum albumin, A/G ratio, SGOT, total bilirubin, prothrombin time, BSP and TTT, there were no significant differences between patients with cytochrome a(+a(3)) contents more than 0.5 x 10(-10) moles per mg protein and those less than 0.5. The results indicate that routine laboratory studies do not have much diagnostic value in estimation of a marked decrease of mitochondrial cytochrome a(+a(3)) contents. It is suggested that the measurements of cytochrome a(+a(3)) of the remnant liver should be done prior to a contemplated major resection.

journal_name

Ann Surg

journal_title

Annals of surgery

authors

Ozawa K,Yamaoka Y,Kitamura O,Nambu H,Kamiyama Y

doi

10.1097/00000658-197412000-00013

subject

Has Abstract

pub_date

1974-12-01 00:00:00

pages

868-76

issue

6

eissn

0003-4932

issn

1528-1140

journal_volume

180

pub_type

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