Abstract:
:Isologous pancreatic islets were implanted into the portal vein of rats with streptozotocin-induced diabetes. At intervals of from one to 32 days after transplantation, the intrahepatic islet grafts were examined histologically and ultrastructurally, and their vascular supply was determined by later perfusion studies. Implanted islets were found widely dispersed throughout the liver in peripheral interlobular portal venules and surrounded by vacuolated liver cells containing large stores of glycogen. The endocrine cells were structurally normal in each interval examined. By the third day after transplantation the beta cells were depleted of secretory granules in aldehyde-fuchsin preparations. Regranulation returned by the 14th day and was associated with secretory organelle hypertrophy and hyperplasia. Islet cells were found outside the portal areas in direct apposition to hepatocytes forming distinct desmosomes by the first day. While hemoperfusion of the grafts occurred from the moment of implantation into the portal venule, a dual vascular supply derived from periportal arterial and venous sources developed by the 11th day after transplantation, establishing full vascularization of the grafts. Preliminary work is presented to show that an active ingrowth of nerves in the islet graft occurs in association with the process of vascularization.
journal_name
Diabetesjournal_title
Diabetesauthors
Griffith RC,Scharp DW,Hartman BK,Ballinger WF,Lacy PEdoi
10.2337/diab.26.3.201subject
Has Abstractpub_date
1977-03-01 00:00:00pages
201-14issue
3eissn
0012-1797issn
1939-327Xjournal_volume
26pub_type
杂志文章相关文献
DIABETES文献大全abstract::Diabetes is generally diagnosed too late. Therefore, biomarkers indicating early stages of β-cell dysfunction and mass reduction would facilitate timely counteraction. Transgenic pigs expressing a dominant-negative glucose-dependent insulinotropic polypeptide receptor (GIPR(dn)) reveal progressive deterioration of glu...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-1133
更新日期:2012-08-01 00:00:00
abstract::Sodium-glucose cotransporter 2 (SGLT2) inhibitors are the most recently approved class of diabetes drugs. Unlike other agents, SGLT2 inhibitors act on the kidney to promote urinary glucose excretion. SGLT2 inhibitors provide multiple benefits, including decreased HbA1c, body weight, and blood pressure. These drugs hav...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/dbi18-0006
更新日期:2019-06-01 00:00:00
abstract::A corpus of evidence suggests that T-helper type 1 (Th1)-dependent cellular immunity plays a pivotal role in the pathogenesis of autoimmune diabetes. This study was intended to find ways to prevent the development of NOD diabetes using a neutralizing anti-interleukin (IL)-12 antibody (C17.8) that inhibits Thl cell dif...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.12.1998
更新日期:2000-12-01 00:00:00
abstract::Mutations in several genes cause nonautoimmune diabetes, but numerous patients still have unclear genetic defects, hampering our understanding of the development of the disease and preventing pathogenesis-oriented treatment. We used whole-genome sequencing with linkage analysis to study a consanguineous family with ea...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-1784
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:Circulating glucose inhibits glucose production in normal rodents and humans, but this glucose effectiveness is disrupted in diabetes due partly to sustained hyperglycemia. We hypothesize that hyperglycemia in diabetes impairs hypothalamic glucose sensing to lower glucose production, and changes of glucose tr...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-0120
更新日期:2011-07-01 00:00:00
abstract::To determine the effect of inhibition of gluconeogenesis on liver glycogen stores in patients with non-insulin-dependent diabetes mellitus (NIDDM) after a 3-day fast, 10% ethanol (EtOH) was administered intravenously to nine obese patients with NIDDM and six obese nondiabetic subjects. Rates of glucose appearance (3-[...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.2.256
更新日期:1994-02-01 00:00:00
abstract::The Dio2 gene encodes the type 2 deiodinase (D2) that activates thyroxine (T4) to 3,3',5-triiodothyronine (T3), the disruption of which (Dio2(-/-)) results in brown adipose tissue (BAT)-specific hypothyroidism in an otherwise euthyroid animal. In the present studies, cold exposure increased Dio2(-/-) BAT sympathetic s...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.3.577
更新日期:2004-03-01 00:00:00
abstract::The incretin effect denominates the phenomenon that oral glucose elicits a higher insulin response than does intravenous glucose. The two hormones responsible for the incretin effect, glucose-dependent insulinotropic hormone (GIP) and glucagon-like peptide-1 (GLP-1), are secreted after oral glucose loads and augment i...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.53.suppl_3.s190
更新日期:2004-12-01 00:00:00
abstract::Concentrations of glycosylated hemoglobin (GHb) are elevated in diabetes mellitus and are believed to reflect previous metabolic control. To better define possible determinants of GHb in man, we investigated the relationship between GHb and both fasting plasma glucose (FPG) and basal insulin (IRI) in 42 normal subject...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.27.8.834
更新日期:1978-08-01 00:00:00
abstract::Twelve type I (insulin-dependent) diabetic subjects in stable metabolic control for at least 3 mo received a controlled diet containing 50% carbohydrate, 35% fat, and 15% protein. Calorie intake varied from 1800 to 2200 calories, depending on individual needs. Part of the polyunsaturated omega-6 fatty acids (omega 6FA...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.11.1412
更新日期:1989-11-01 00:00:00
abstract::The past decade has witnessed an exponential increase in our ability to search the genome for genetic factors predisposing to cardiovascular disease (CVD) and in particular coronary heart disease (CHD). Identifying these genes could lead to the development of innovative strategies to prevent the cardiovascular complic...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/dbi18-0036
更新日期:2019-03-01 00:00:00
abstract::This article is divided into two parts. A retrospective overview summarizes some of the work that provided the framework and tools of the more recent studies. The five novel areas of research are related to the indirect effects of insulin. Regulation of plasma glucose is of central importance in health and diabetes. U...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diab.41.9.1188
更新日期:1992-09-01 00:00:00
abstract::Although diabetes is the most common cause of end-stage renal disease (ESRD) worldwide, most people with diabetic nephropathy will never develop ESRD but will instead die of cardiovascular (CV) disease (CVD). The first evidence of kidney injury in diabetes is often microalbuminuria, itself also an independent risk mar...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-0795
更新日期:2013-11-01 00:00:00
abstract::Cardiovascular diseases are the leading cause of morbidity and mortality in diabetes. Lipoprotein lipase (LPL), a major secretory product of macrophages, has been suggested to play a key role in the development of atherosclerosis. In the present study, we evaluated the effect of high glucose on macrophage LPL mRNA exp...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.47.3.431
更新日期:1998-03-01 00:00:00
abstract::Obesity and dysfunctional energy partitioning can lead to the development of insulin resistance and type 2 diabetes. The antidiabetic thiazolidinediones shift the energy balance toward storage, leading to an increase in whole-body adiposity. These studies examine the effects of pioglitazone (Pio) on adipose tissue phy...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.8.1863
更新日期:2001-08-01 00:00:00
abstract::Recently, a missense mutation replacing tryptophan with arginine at codon 64 of the beta 3-adrenergic receptor gene was shown to be associated with insulin resistance in nondiabetic subjects and to an earlier onset of NIDDM in Pima Indians. We studied whether the codon 64 amino acid polymorphism of the beta 3-adrenerg...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.45.8.1115
更新日期:1996-08-01 00:00:00
abstract::Thiazolidinediones (TZDs) are peroxisome proliferator-activated receptor-gamma (PPARG) agonists used to treat type 2 diabetes. TZDs can also be used to reduce rates of type 2 diabetes in at-risk individuals. However, a large fraction of TZD-treated patients (30-40%) do not respond to TZD treatment with an improvement ...
journal_title:Diabetes
pub_type: 杂志文章,随机对照试验
doi:10.2337/diabetes.54.11.3319
更新日期:2005-11-01 00:00:00
abstract::Neogenesis is crucial for the maintenance of beta-cell mass in the human pancreas and possibly for the outcome of clinical islet transplantation. To date, no studies have reported a stimulation of human beta-cell neogenesis in vivo. Therefore, we investigated whether human alpha-, beta-, and duct cell growth can be st...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.2.301
更新日期:2001-02-01 00:00:00
abstract::It is well established that patients with non-insulin-dependent diabetes mellitus (NIDDM) are resistant to insulin. However, the contribution of hepatic and extrahepatic tissues to insulin resistance remains controversial. The uncertainty may be at least in part due to errors introduced by the unknowing use in previou...
journal_title:Diabetes
pub_type: 临床试验,杂志文章
doi:10.2337/diab.39.2.217
更新日期:1990-02-01 00:00:00
abstract::In this study, 92% of patients' serums known to contain antibodies against islet cells, including the Juvenile Diabetes Foundation provisional reference serum, had antibodies reacting with gastrointestinal carcinoid tumors. Twelve percent of the control serums from healthy individuals bound to carcinoid cells, and 2% ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.5.667
更新日期:1989-05-01 00:00:00
abstract::ATP is known to be coreleased with insulin from pancreatic beta-cells. To monitor insulin secretion from single beta-cells, a single beta-cell was surrounded in culture by Fura 2-loaded calf pulmonary artery endothelium (CPAE) cells, which can detect the ATP. CPAE cells did not respond with an elevation in cytoplasmic...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.44.10.1213
更新日期:1995-10-01 00:00:00
abstract::Glucose stimulates insulin secretion by generating triggering and amplifying signals in beta-cells. The triggering pathway is well characterized. It involves the following sequence of events: entry of glucose by facilitated diffusion, metabolism of glucose by oxidative glycolysis, rise in the ATP-to-ADP ratio, closure...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.49.11.1751
更新日期:2000-11-01 00:00:00
abstract::Non-insulin-dependent diabetes mellitus (NIDDM) is a heterogenous disorder characterized by defects in insulin action and secretion. This study was aimed at developing a rat model in which these pathogenic factors might be studied. Male Wistar rats were injected at 2 days of age with 45 or 30 mg/kg streptozocin (STZ) ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.39.2.226
更新日期:1990-02-01 00:00:00
abstract::Rat islets contain the acid-labile activator of phosphofructokinase, fructose-2,6-bisphosphate. The islet content in activator is higher in islets exposed to glucose (16.7 mM) than in islets deprived of glucose. The islets display fructose-6-phosphate, 2-kinase activity with a Km for fructose-6-phosphate close to 0.08...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.31.1.90
更新日期:1982-01-01 00:00:00
abstract::Although many studies using rodent islets and insulinoma cell lines have been performed to determine the role of insulin in the regulation of islet function, the autocrine effect of insulin on insulin gene expression is still controversial, and no consensus has yet been achieved. Because very little is known about the...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0532
更新日期:2006-10-01 00:00:00
abstract::The farnesoid X receptor (FXR) and the liver x receptors (LXRs) are bile acid-activated receptors that are highly expressed in the enterohepatic tissues. The mechanisms that support the beneficial effects of bariatric surgery are only partially defined. We have investigated the effects of ileal interposition (IT), a s...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-0299
更新日期:2013-10-01 00:00:00
abstract::Accumulation of triosephosphates arising from high cytosolic glucose concentrations in hyperglycemia is the trigger for biochemical dysfunction leading to the development of diabetic nephropathy-a common complication of diabetes associated with a high risk of cardiovascular disease and mortality. Here we report that s...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.8.2110
更新日期:2003-08-01 00:00:00
abstract::This study examines whether the loss of metabolic control in initially normalized islet transplants can result from the inadequate composition of the donor tissue. Streptozotocin-induced diabetic rats were followed for 64 weeks after the intraportal injection of islet isografts with different composition. The implanta...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.45.12.1814
更新日期:1996-12-01 00:00:00
abstract::The transcription factor regulatory factor X (RFX)-3 regulates the expression of genes required for the growth and function of cilia. We show here that mouse RFX3 is expressed in developing and mature pancreatic endocrine cells during embryogenesis and in adults. RFX3 expression already is evident in early Ngn3-positi...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-1187
更新日期:2007-04-01 00:00:00
abstract::In peripheral tissues, insulin signaling involves activation of the insulin receptor substrate (IRS)-phosphatidylinositol 3-kinase (PI3K) enzyme system. In the hypothalamus, insulin functions with leptin as an afferent adiposity signal important for the regulation of body fat stores and hepatic glucose metabolism. To ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.2.227
更新日期:2003-02-01 00:00:00