Abstract:
:DNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reaction were exploited for their applications in the differentiation of the traditional chinese medicinal plants euphorbia humifusa and e. maculata from three related adulterants e. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. humifusa and E. maculata from the adulterants by TaqMan real-time PCR.
journal_name
Planta Medjournal_title
Planta medicaauthors
Xue HG,Wang H,Li DZ,Xue CY,Wang QZdoi
10.1055/s-2008-1034305subject
Has Abstractpub_date
2008-02-01 00:00:00pages
302-4issue
3eissn
0032-0943issn
1439-0221journal_volume
74pub_type
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