Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction.

Abstract:

:DNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reaction were exploited for their applications in the differentiation of the traditional chinese medicinal plants euphorbia humifusa and e. maculata from three related adulterants e. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. humifusa and E. maculata from the adulterants by TaqMan real-time PCR.

journal_name

Planta Med

journal_title

Planta medica

authors

Xue HG,Wang H,Li DZ,Xue CY,Wang QZ

doi

10.1055/s-2008-1034305

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

302-4

issue

3

eissn

0032-0943

issn

1439-0221

journal_volume

74

pub_type

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