Excitation of lateral horn neurons of the neonatal rat spinal cord by 5-hydroxytryptamine.


:The effects of 5-hydroxytryptamine (5-HT) on lateral horn cells contained in thin in vitro slices of neonatal rat spinal cord were investigated by means of intracellular recording techniques. Superfusion of 5-HT (1-100 microM) to lateral horn cells caused a concentration-dependent membrane depolarization leading to, in some instances, repetitive cell discharges. A number of lateral horn cells could be activated antidromically by stimulating the ventral rootlets. The conduction velocity of the antidromic spikes was estimated to be 0.3-2 m/s which corresponds to that of the axons of rat sympathetic preganglionic neurons reported by others. The 5-HT depolarization evoked in neurons that could be activated antidromically was similar to that elicited from unidentified lateral horn cells. The depolarization induced by 5-HT could be partially eliminated by low Ca/high Mg solution or tetrodotoxin in a portion of lateral horn cells and was accompanied by an increase in membrane resistance. The response was nullified near the membrane potential at which the spike after hyperpolarization was abolished; a clear reversal of polarity was not observed at a more negative potential level. The 5-HT depolarization was reversibly blocked by methysergide and cyproheptadine and enhanced by fluoxetine, a 5-HT-uptake inhibitor. The results indicate that the indoleamine primarily exerted an excitatory action on lateral horn cells, including those tentatively identified as sympathetic preganglionic neurons, by a direct depolarization which appears to be mediated by decrease of a voltage-sensitive K conductance and partly by an indirect effect via the release of an excitatory substance(s).


Brain Res


Brain research


Ma RC,Dun NJ




Has Abstract


1986-01-01 00:00:00












  • Differences between strychnine and penicillin epileptogenesis suggest a laminar organization of neocortical inhibition.

    abstract::Transient foci of epileptiform alteration in neuronal population activity were induced by microinjection of strychnine sulfate into different layers of cat striate cortex. Potentials evoked by visual field-specific photic stimulation were recorded from microelectrodes at the injection site and in adjacent laminae. Epi...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ebersole JS,Chatt AB

    更新日期:1985-08-12 00:00:00

  • Release and spread of immunoreactive neurokinin A in the cat spinal cord in a model of acute arthritis.

    abstract::Antibody microprobes were used to study the release of immunoreactive neurokinin A into the spinal cord of anaesthetised cats during and following injection of a knee joint with kaolin and carrageenan. A basal level of immunoreactive neurokinin A was detected prior to any noxious stimuli. Innocuous mechanical joint st...

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    pub_type: 杂志文章


    authors: Hope PJ,Jarrott B,Schaible HG,Clarke RW,Duggan AW

    更新日期:1990-11-19 00:00:00

  • GFP-based FRET analysis in live cells.

    abstract::Fluorescence resonance energy transfer (FRET) is a widely utilized optical technique for measuring small distances of 1-10 nm in live cells. In recent years, its application has been greatly popularized by the discovery of green fluorescent protein (GFP) and many improved variants which make good donor-acceptor fluoro...

    journal_title:Brain research

    pub_type: 杂志文章,评审


    authors: Takanishi CL,Bykova EA,Cheng W,Zheng J

    更新日期:2006-05-26 00:00:00

  • miR-106b aberrantly expressed in a double transgenic mouse model for Alzheimer's disease targets TGF-β type II receptor.

    abstract::MicroRNAs (miRNAs) are abundantly expressed in the brain and play an important role in disorders of the brain, including Alzheimer's diseases (AD). Growing body of evidence suggests that the TGF-β signaling pathway plays a key role in the pathogenesis of AD. However, it is unclear whether miRNAs involved in AD pathoge...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wang H,Liu J,Zong Y,Xu Y,Deng W,Zhu H,Liu Y,Ma C,Huang L,Zhang L,Qin C

    更新日期:2010-10-21 00:00:00

  • CNTF potentiates peripheral nerve regeneration.

    abstract::The effect of ciliary neurotrophic factor (CNTF) on peripheral nerve regeneration was studied in 6-week-old rats following sciatic nerve transection and juxtaposition of proximal and distal stumps. Rats received intraperitoneal recombinant human CNTF, 1 mg/kg every other day for 12 days. In all CNTF-treated animals, t...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Sahenk Z,Seharaseyon J,Mendell JR

    更新日期:1994-08-29 00:00:00

  • Reduced kainic acid binding in rat hippocampal formation after limbic kindling.

    abstract::The specific binding of [3H]kainic acid to hippocampal membranes was examined autoradiographically in rats kindled by tetanic stimulation of the amygdala or angular bundle. One day after the last of 3 class 4-5 kindled seizures, the specific binding of [3H]kainic acid in stratum lucidum of area CA3 was 47-61% less tha...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Savage DD,Nadler JV,McNamara JO

    更新日期:1984-12-03 00:00:00

  • Environmental enrichment restores cognitive deficits induced by prenatal maternal seizure.

    abstract::Maternal seizure has adverse effects on brain histology as well as on learning and memory ability in progeny. An enriched environment (EE) is known to promote structural changes in the brain and improve cognitive and motor deficits following a variety of brain injuries. Whether EE treatment in early postnatal periods ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Xie T,Wang WP,Jia LJ,Mao ZF,Qu ZZ,Luan SQ,Kan MC

    更新日期:2012-08-27 00:00:00

  • Spontaneous and odour evoked activity in single avian olfactory bulb neurones.

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    journal_title:Brain research

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Casey KL,Morrow TJ

    更新日期:1989-10-30 00:00:00

  • Role of anterior pretectal nucleus in somatosensory cortical descending modulation of jaw-opening reflex in rats.

    abstract::Both the somatosensory cerebral cortex and anterior pretectal nucleus (APT) have been shown to produce descending modulation of trigeminal (V) and spinal somatosensory neurone and reflex activities. Since the APT receives a direct projection from the somatosensory cortex, experiments were performed to compare the effe...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Chiang CY,Dostrovsky JO,Sessle BJ

    更新日期:1990-05-07 00:00:00

  • Dietary long chain PUFAs differentially affect hippocampal muscarinic 1 and serotonergic 1A receptors in experimental cerebral hypoperfusion.

    abstract::The chronic dietary intake of essential polyunsaturated fatty acids (PUFAs) can modulate learning and memory by being incorporated into neuronal plasma membranes. Representatives of two PUFA families, the n-3 and n-6 types become integrated into membrane phospholipids, where the actual (n-6)/(n-3) ratio can determine ...

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    更新日期:2002-11-01 00:00:00

  • Development and selective neurodegeneration in cell cultures from different hippocampal regions.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Mattson MP,Kater SB

    更新日期:1989-06-19 00:00:00

  • Hypothalamic substrates for brain stimulation-induced patterns of locomotion and escape jumps in the rat.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Lammers JH,Kruk MR,Meelis W,van der Poel AM

    更新日期:1988-05-24 00:00:00

  • Effect of cocaine on the 5-HT3 receptor-mediated ion current in Xenopus oocytes.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Fan P,Oz M,Zhang L,Weight FF

    更新日期:1995-03-06 00:00:00

  • From sublexical facilitation to lexical competition: ERP effects of masked neighbor priming.

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    pub_type: 杂志文章


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    更新日期:1988-07-26 00:00:00

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    pub_type: 杂志文章


    authors: Vinogradova LV

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    journal_title:Brain research

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    abstract::Hamsters exhibited a circadian variation in metabolic activity determined by [14C]2-deoxyglucose uptake in the suprachiasmatic nuclei, as has been previously reported for rats. The 13-line ground squirrel, a diurnal rodent, did not show a circadian variation in metabolic activity. The data are consistent with the hypo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Flood DG,Gibbs FP

    更新日期:1982-01-28 00:00:00

  • Electrophysiological and morphological studies on thalamic neurons receiving entopedunculo- and cerebello-thalamic projections in the cat.

    abstract::Electrophysiological studies on the entopedunculo- and cerebello-thalamic projections were performed by intracellular recordings in the thalamic VA, VL and VM nuclei of cats under sodium pentobarbital anesthesia. Identification of the thalamic neurons were performed electrophysiologically by antidromic activation on s...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Yamamoto T,Noda T,Miyata M,Nishimura Y

    更新日期:1984-06-03 00:00:00

  • Ganglioside-mediated enhancement of the cytoskeletal organization and activity in neuro-2a neuroblastoma cells.

    abstract::Our previous studies have demonstrated that a mixture of bovine brain gangliosides ( BBG ) applied to Neuro-2a neuroblastoma cells markedly increased the degree and rate of neurite formation. In the present study, the cytoskeletal basis for BBG -mediated neurite outgrowth was investigated by comparing cells grown in t...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Spero DA,Roisen FJ

    更新日期:1984-03-01 00:00:00

  • Ontogenesis of chromogranin A and B and catecholamines in rat adrenal medulla.

    abstract::The ontogenesis of chromogranins A and B and catecholamines was investigated for rat adrenal medulla. The chromogranins, the major secretory peptides of chromaffin granules, were characterized by one- and two-dimensional immunoblotting. Chromogranin B appeared identical in fetal and postnatal adrenals. On the other ha...

    journal_title:Brain research

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    authors: Schober M,Fischer-Colbrie R,Winkler H

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  • Axonal cytoskeleton changes in experimental optic neuritis.

    abstract::Axonal loss and degeneration in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE) have been suggested by brain imaging, pathological and axonal transport studies. Further elucidation of the processes and mechanisms of axonal degeneration in demyelinating diseases is therefore of potential impor...

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    authors: Zhu B,Moore GR,Zwimpfer TJ,Kastrukoff LF,Dyer JK,Steeves JD,Paty DW,Cynader MS

    更新日期:1999-04-10 00:00:00

  • Is taurine a neurotransmitter in rabbit retina?

    abstract::Rabbit retina was used as a model to study the possible role of taurine in the retina. The taurine-synthesizing enzyme, cysteine sulfinic acid decarboxylase (CSAD), is localized immunohistochemically using specific antibodies against CSAD. The CSAD-immunoreactivity appears to be most prominent in the inner nuclear lay...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Lin CT,Song GX,Wu JY

    更新日期:1985-07-01 00:00:00

  • Is the effect of somatostatin on muscarinic receptors selective to M1 type?

    abstract::The effect of somatostatin on muscarinic receptors (mAchR) was investigated through saturation experiments of [3H]oxotremorine-M-acetate and oxotremorine/[3H]N-methyl-scopolamine competition experiments. Somatostatin converted oxotremorine high affinity binding sites to low affinity sites in the hippocampus and cerebr...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Miyoshi R,Kito S,Mizuno K,Matsubayashi H

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  • Oxygen-induced apoptosis in PC12 cells with special reference to the role of Bcl-2.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kubo T,Enokido Y,Yamada M,Oka T,Uchiyama Y,Hatanaka H

    更新日期:1996-09-16 00:00:00

  • Evidence for the involvement of descending noradrenergic pathways in the antinociceptive effect of baclofen.

    abstract::The role of descending monoaminergic pathways in the antinociceptive effect of baclofen following intraperitoneal (i.p.) administration was investigated by determining the effect of intrathecal (i.t.) administration of neurotoxins [6-hydroxydopamine (6-OHDA) and 5,6-dihydroxytryptamine (5,6-DHT)] and receptor antagoni...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Sawynok J,Dickson C

    更新日期:1985-05-27 00:00:00

  • Synapse formation after injury in the adult rat brain: preferential reinnervation of denervated fimbrial sites by axons of the contralateral fimbria.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Field PM,Coldham DE,Raisman G

    更新日期:1980-05-05 00:00:00

  • Prolactin response to morphine in intact and adrenalectomized lactating rats.

    abstract::To examine the hypothesis that the increased adrenocortical activity during lactation induced the loss of the prolactin (PRL) -releasing effect of morphine, we studied the effect of morphine in adrenalectomized (ADX) and sham-operated primiparous lactating Wistar rats. Animals were adrenalectomized 4 days after delive...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kiem DT,Fekete MI,Bartha L,Nagy G,Makara GB

    更新日期:1991-11-01 00:00:00

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Zhou Y,Jin G,Mi R,Dong C,Zhang J,Liu F

    更新日期:2014-03-27 00:00:00