Abstract:
:Long-term insulin secretion was investigated in seven biohybrid capillary devices seeded with canine islets. Approximately 50,000 islets could be isolated from a single canine pancreas using collagenase digestion in conjunction with the recently described Velcro technique. Devices were perfused with tissue culture medium 199 containing 300 mg/dl glucose. Insulin secretion fell during the first 1-2 days of culture from approximately 18 to 6 U/day. After 3-4 wk of perfusion, however, there was a gradual rise in insulin output that reached greater than 15 U/day after 7 wk. Insulin output eventually stabilized at 18-20 U/day. Two of these devices were studied for 80 and 86 days, respectively, and continued to secrete these same amounts of insulin. Glucose-induced insulin release was studied after 1 and 4 wk of culture and was well preserved.
journal_name
Diabetesjournal_title
Diabetesauthors
Araki Y,Solomon BA,Basile RM,Chick WLdoi
10.2337/diab.34.9.850subject
Has Abstractpub_date
1985-09-01 00:00:00pages
850-4issue
9eissn
0012-1797issn
1939-327Xjournal_volume
34pub_type
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