Abstract:
:The Dazl gene encodes a germ-cell-specific RNA-binding protein which is essential for spermatogenesis. It has been proposed that this protein (DAZL) binds to RNA in the cytoplasm of germ cells and controls spermatogenesis. Using the specific nucleic acids associated with proteins (SNAAP) technique, we identified 17 target mRNAs bound by mDAZL. Among these transcripts, we focused on TSSK2, which encodes a testis-specific serine/threonine kinase. To date, five TSSK family members have been cloned, and all are exclusively expressed in the testis. We demonstrated that in addition to the TSSK1 3'UTR , the 3'UTRs of TSSKs 2 and 4 were bound by human and mouse DAZL, and that human DAZL (hDAZL) bound to the 3'UTR of human TSSK5 (hTSSK5). Our results suggest that the Dazl gene may play different roles in human and mouse spermatogenesis by regulating different members of the downstream gene family.
journal_name
BMB Repjournal_title
BMB reportsauthors
Zeng M,Deng W,Wang X,Qiu W,Liu Y,Sun H,Tao D,Zhang S,Ma Ydoi
10.5483/bmbrep.2008.41.4.300subject
Has Abstractpub_date
2008-04-30 00:00:00pages
300-4issue
4eissn
1976-6696issn
1976-670Xjournal_volume
41pub_type
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