当前位置： SCI文献检索 > JOURNAL OF PEDIATRIC SURGERY期刊下所有文献 > Laser microdissection allows detection of abnormal gene expression in cystic adenomatoid malformation of the lung.

Laser microdissection allows detection of abnormal gene expression in cystic adenomatoid malformation of the lung.

Abstract：

BACKGROUND/PURPOSE:Congenital cystic adenomatoid malformation (CCAM) of the lung may result from a localized aberrant epithelial-mesenchymal interaction during lung development. We used laser microdissection (LMD) to isolate the epithelium and mesenchyme of CCAM, and studied candidate gene expression in these pure cell populations. METHODS:Congenital cystic adenomatoid malformation tissue was obtained from fetal (n = 5) and postnatal (n = 5) surgical specimens. Normal fetal lung (n = 10) was obtained from abortus material, and normal postnatal lung (n = 5) was identified from surgical specimens. Whole tissue was analyzed using immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR). Using LMD, columnar bronchiolar type epithelium and underlying mesenchyme were isolated. Multiplex nested RT-PCR was then used to detect message levels of candidate genes. RESULTS:Reverse transcriptase polymerase chain reaction performed on LMD-isolated tissue, but not whole tissue homogenate, revealed differences between CCAM and normal lung. In this report, we focus on the fibroblast growth factor (FGF) family. By RT-PCR, there was 4-fold more epithelial expression of FGF9 in fetal CCAM vs normal fetal lung (P < .07). This was qualitatively confirmed by immunohistochemistry. We also detected decreased FGF7 expression in CCAM mesenchyme (P < .05) but no significant differences in FGF10 or FGFR2. CONCLUSIONS:LMD may be used to overcome the limitations of tissue heterogeneity in the study of CCAM. Abnormal growth factor expression may play a role in the etiology of this lesion.

journal_name

J Pediatr Surg

journal_title

Journal of pediatric surgery

authors

Jancelewicz T,Nobuhara K,Hawgood S

doi

10.1016/j.jpedsurg.2008.02.027

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

1044-51

issue

6

eissn

0022-3468

issn

1531-5037

pii

S0022-3468(08)00164-4

journal_volume

43

pub_type

杂志文章

相关文献

JOURNAL OF PEDIATRIC SURGERY文献大全
  • Anatomic evidence for coexistence of cholinergic and adrenergic neurons in the developing human intestine: new aspects in the pathogenesis of developmental neuronal abnormalities.

    abstract：:In order to interpret the interrelation of cholinergic and adrenergic myenteric neural elements in the developing human, histochemical methods were used to demonstrate cholinergic and adrenergic activity independently in consecutive cryostat sections of the esophagus, ileocecal region, and colon of fetuses of 9 to 22 ...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/s0022-3468(88)80729-2

    authors： Vaos GC,Lister J

    更新日期：1988-03-01 00:00:00

  • Extracorporeal membrane oxygenation as a lifesaving modality in the treatment of pediatric patients with burns and respiratory failure.

    abstract：PURPOSE:Several case series have described successful utilization of extracorporeal membrane oxygenation (ECMO) for the treatment of pediatric burn patients with respiratory failure. This study examines the Extracorporeal Life Support Organization registry experience in the treatment of these patients. METHODS:The Ext...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jpedsurg.2010.02.106

    authors： Askegard-Giesmann JR,Besner GE,Fabia R,Caniano DA,Preston T,Kenney BD

    更新日期：2010-06-01 00:00:00

  • Precocious puberty because of a pancreatic neuroectodermal tumor.

    abstract：:Primitive neuroectodermal tumors (PNETs) are uncommon, representing approximately 1% of all sarcomas. Pancreatic PNETs have been reported only 12 times in the literature. This report presents the first case of a patient with a PNET exhibiting precocious puberty. The clinical presentation, surgical management, and path...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2006.05.046

    authors： Schutte WP,Knight PJ

    更新日期：2006-11-01 00:00:00

  • Malignant risk in juvenile polyposis coli: increasing documentation in the pediatric age group.

    abstract：:The presence of juvenile polyps with resulting bleeding and abdominal pain has traditionally been considered a benign, self-limiting process which would resolve with age. The dictum that these polyps were usually solitary, were found predominantly in the rectosigmoid area, and were without malignant potential has been...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/0022-3468(93)90162-e

    authors： Heiss KF,Schaffner D,Ricketts RR,Winn K

    更新日期：1993-09-01 00:00:00

  • Gastroduodenal mucosal intussusception causing gastric outlet obstruction: a complication of gastrostomy tubes.

    abstract：:Acquired gastric outlet obstruction occurred in a neonate following gastrostomy, after surgery to salvage a dehisced primary repair of an esophageal atresia. Despite free drainage of the gastrostomy and total support of the neonate, the gastric outlet obstruction persisted and required a further operation. An anterogr...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/s0022-3468(88)80400-7

    authors： Galea MH,Mayell MJ

    更新日期：1988-10-01 00:00:00

  • Increased pulmonary RhoA expression in the nitrofen-induced congenital diaphragmatic hernia rat model.

    abstract：PURPOSE:Persistent pulmonary hypertension remains a major cause of mortality and morbidity in cases of congenital diaphragmatic hernia (CDH). Recently, RhoA/Rho-kinase-mediated vasoconstriction has been reported to be important in the pathogenesis of pulmonary hypertension (PH). Several recent reports have described th...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2015.02.063

    authors： Takayasu H,Masumoto K,Hagiwara K,Sasaki T,Ono K,Jimbo T,Uesugi T,Gotoh C,Urita Y,Shinkai T,Tanaka H

    更新日期：2015-09-01 00:00:00

  • Persistent Mullerian duct syndrome: report of two boys with associated crossed testicular ectopia.

    abstract：:Persistent Mullerian duct structures and crossed testicular ectopia were found in two phenotypically normal, unrelated males, with 46,XY karyotype, during routine herniorrhaphy. In each case, the vascular supply to the ectopic testis originated from the appropriate ipsilateral side. The clinical significance and genet...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/0022-3468(90)90367-i

    authors： Mahfouz ES,Issa MA,Farag TI,Naguib KK,al-Awadi SA,Schimke RN

    更新日期：1990-06-01 00:00:00

  • Neonatal kaposiform hemangioendothelioma of the spleen associated with Kasabach-Merritt phenomenon.

    abstract：:Kaposiform hemangioendothelioma is a rare locally aggressive vascular tumor that usually manifests during early childhood. Typically the lesion presents with skin, soft tissue and bone involvement and is characterized histologically by ill-defined nodularity and the presence of spindle cells with resemblance to Kaposi...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2016.03.014

    authors： Shabtaie SA,Wang B,Owyong M,Ruiz-Mesa C,Corrales-Medina FF,Rojas CP,Infante JC,Neville HL,Perez EA,Sola JE,Hogan AR

    更新日期：2016-06-01 00:00:00

  • Successful separation of a conjoined biliary tract in a set of omphalopagus twins.

    abstract：:Omphalopagus twins were separated in the first 48 hours of life. Preoperative evaluation did not disclose either their conjoined intestines or their conjoined biliary tract; intraoperative evaluation, including an operative cholangiogram, was required to determine if separation was possible. The intestines were divide...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/s0022-3468(89)80598-6

    authors： Lobe TE,Oldham KT,Richardson CJ

    更新日期：1989-09-01 00:00:00

  • Congenital heart disease and heterotaxy: upper gastrointestinal fluoroscopy can be misleading and surgery in an asymptomatic patient is not beneficial.

    abstract：PURPOSE:Heterotaxy syndrome is associated with intestinal abnormalities. We sought to define the gastrointestinal anatomy and determine both the risk of volvulus and benefit of screening upper gastrointestinal fluoroscopy (UGI) in these patients. METHODS:Medical records from 2003 until 2011 at Children's Hospital Los ...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2012.10.033

    authors： Papillon S,Goodhue CJ,Zmora O,Sharma SS,Wells WJ,Ford HR,Upperman JS,Wang KS,Bushman GA,Kim R,Pierce JR

    更新日期：2013-01-01 00:00:00

  • Unique protein expression by the TBJ clonal derivative of C1300 murine neuroblastoma.

    abstract：:C1300 is a murine neuroblastoma that arose spontaneously in an A/JAX mouse, and from which a clone termed TBJ was subsequently derived. C1300 is a slowly growing and poorly metastasizing tumor, whereas TBJ shows early systemic metastasis as well as aggressive local growth. Compared with TBJ cells, C1300 cells are high...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/0022-3468(95)90448-4

    authors： Vegunta RK,Cooney DR,Downing MT,Besner GE,Brigstock DR

    更新日期：1995-12-01 00:00:00

  • Endoscopic management of recurrent tracheoesophageal fistula.

    abstract：RATIONALE:Recurrent tracheoesophageal fistulas (RTEFs) remain a therapeutic challenge because open surgical approaches have been associated with substantial rates of morbidity, mortality, and repeat recurrences. Recently, endoscopic techniques for the repair of RTEF have provided an alternative approach with the potent...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2007.08.062

    authors： Richter GT,Ryckman F,Brown RL,Rutter MJ

    更新日期：2008-01-01 00:00:00

  • The impact of variation in access to care on the management of Hirschsprung disease.

    abstract：INTRODUCTION:Variation in access to care has a significant impact on the disease management process and outcomes. Variable access to care might have similar effects on the management of Hirschsprung disease (HD). However, such variation has not been highlighted. MATERIALS AND METHODS:All patients referred to 3 academi...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jpedsurg.2012.01.052

    authors： Al-Jazaeri A,Al-Shanafey S,Zamakhshary M,Al-Jarbou W,Hajr E,Breakeit M,Qutob S

    更新日期：2012-05-01 00:00:00

  • Efficacy of video-assisted thoracoscopic surgery in managing childhood empyema: a large single-centre study.

    abstract：BACKGROUND/PURPOSE:A randomised controlled trial evaluating the role of video-assisted thoracoscopic surgery (VATS) in childhood empyema reported a failure rate of 16.6%. Our aim is to determine the outcome of VATS in a large series of children managed by 3 paediatric surgeons experienced in endoscopic surgery. METHOD...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2008.10.083

    authors： Bishay M,Short M,Shah K,Nagraj S,Arul S,Parikh D,Jawaheer G

    更新日期：2009-02-01 00:00:00

  • Community distress predicts youth gun violence.

    abstract：BACKGROUND:The purpose of this study was to investigate our institution's experience with pediatric firearm events. We sought to determine the relationship between a community's level of socioeconomic distress and the incidence of youth gun violence. METHODS:We performed a retrospective review of children <18 years in...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2019.03.021

    authors： Tracy BM,Smith RN,Miller K,Clayton E,Bailey K,Gerrin C,Eversley-Kelso T,Carney D,MacNew H

    更新日期：2019-11-01 00:00:00

  • Multicystic dysplastic kidney - treat each case on its merits.

    abstract：OBJECTIVES:To assess outcomes of unilateral multicystic dysplastic kidney (MCDK) managed at an Australasian centre over a 15 year period. To assess if MCDK involution could be predicted based on change noted between first two postnatal ultrasound scans 6 months apart. SUBJECTS AND METHODS:A retrospective study was per...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2019.12.008

    authors： Faruque A,Narayanan S,Marley I,Lai C,Khosa J,Barker A,Samnakay N

    更新日期：2020-11-01 00:00:00

  • Neuroblastoma: outcome over a 14 year period from a tertiary care referral centre in India.

    abstract：PURPOSE:To evaluate the outcome of children with neuroblastoma (NB) from a tertiary care referral centre in India. METHOD:All children with NB registered from October 1996 through July 2009 were included in the study. INSS was used for staging. All children included in the study received chemotherapy and radiation the...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2014.03.017

    authors： Agarwala S,Mandelia A,Bakhshi S,Srinivas M,Bajpai M,Gupta AK,Gupta DK,Bhatnagar V

    更新日期：2014-08-01 00:00:00

  • External caliper-based measurements of the modified percent depth as an alternative to cross-sectional imaging for assessing the severity of pectus excavatum.

    abstract：BACKGROUND:Cross-sectional imaging (CSI) may be clinically unnecessary in the evaluation of pectus excavatum (PE). The purpose of our study was to prospectively evaluate the accuracy and reliability of the modified percent depth (MPD), derived from caliper-based external measurements, in identifying PE. METHODS:Childr...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jpedsurg.2020.02.053

    authors： Bludevich BM,Kauffman JD,Litz CN,Farach SM,DeRosa JC,Wharton K,Potthast K,Danielson PD,Snyder CW,Chandler NM

    更新日期：2020-06-01 00:00:00

  • Case report: successful interdisciplinary treatment of cerebrally disseminated invasive pulmonal aspergillosis in a child with acute myeloid leukemia.

    abstract：:Invasive pulmonary aspergillosis (IPA) is a life-threatening infection in immunocompromised patients. Mortality rates of cerebrally disseminated IPA approach 100%. We report on a case of a 9-year-old girl with acute myeloid leukemia, who acquired cerebrally disseminated IPA during chemotherapy-induced leukopenia. Long...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2005.03.104

    authors： Kurdow R,Boehle AS,Ankermann T,Schniewind B,Dohrmann P

    更新日期：2005-07-01 00:00:00

  • Transabdominal closure of tracheoesophageal fistula: a new procedure for the management of poor-risk esophageal atresia with tracheoesophageal fistula.

    abstract：:Nissen fundoplication results in closure of the tracheoesophageal fistula (TEF) without the necessity for a thoracic operation by preventing both the reflux of gastric contents into the lungs through the distal TEF, and leakage of air into the stomach of less than 30 cm H2O intrapulmonary pressure. This procedure can ...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/s0022-3468(86)80375-x

    authors： Ogita S,Tokiwa K,Takahashi T

    更新日期：1986-09-01 00:00:00

  • Inhibiting hydrogen sulfide production in umbilical stem cells reduces their protective effects during experimental necrotizing enterocolitis.

    abstract：INTRODUCTION:Umbilical mesenchymal stem cells (USC) have been shown to reduce illness in animal models of necrotizing enterocolitis (NEC), possibly through the paracrine release of hydrogen sulfide (H2S). We hypothesized that animals treated with USCs with inhibited H2S synthesis would exhibit more severe disease. MET...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2019.02.037

    authors： Drucker NA,Te Winkel JP,Shelley WC,Olson KR,Markel TA

    更新日期：2019-06-01 00:00:00

  • Cholelithiasis in childhood: a follow-up study.

    abstract：:Fifteen children with cholelithiasis who were treated at the Royal Hospital for Sick Children, Glasgow between 1973 and 1985 are reviewed. Ten patients had idiopathic gallstones and five had gallstones in association with hereditary spherocytosis. All patients have been followed up for between 4 months and 12 years (m...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/s0022-3468(88)80732-2

    authors： Robertson JF,Carachi R,Sweet EM,Raine PA

    更新日期：1988-03-01 00:00:00

  • Cecostomy button for antegrade enemas: survey of 29 patients.

    abstract：OBJECTIVE:This study evaluated the Trap-door button use (Cook Medical, Bloomington, IL) for antegrade enemas in children. METHODS:Since 2002, patients with fecal incontinence or encopresis and constipation underwent percutaneous cecostomy under laparoscopy using a button. Technical details are described. Age at surger...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2008.03.043

    authors： Becmeur F,Demarche M,Lacreuse I,Molinaro F,Kauffmann I,Moog R,Donnars F,Rebeuh J

    更新日期：2008-10-01 00:00:00

  • A case of intrapericardial diaphragmatic hernia with a massive pericardial effusion: fetal diagnosis and therapy.

    abstract：:Intrapericardial diaphragmatic hernia is a very rare phenotype of neonatal diaphragmatic hernia which is thought to be caused by the developmental failure of the septum transversum. There have been only 10 cases reported since 1980, and among them, only 2 cases were diagnosed in fetal life. We herein report a new case...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2005.07.044

    authors： Kanamori Y,Hashizume K,Sugiyama M,Tomonaga T,Goishi K,Yokoyama Y,Igarashi T,Kikuchi A,Kawana Y,Kozuma S,Taketani Y

    更新日期：2005-11-01 00:00:00

  • Comparison of the incidence of complications in open and laparoscopic pyloromyotomy: a concurrent single institution series.

    abstract：PURPOSE:The purpose of this study was to compare the incidence and type of technical complications seen in a concurrent series of pyloromyotomies done open and laparoscopically. METHODS:The medical records of all patients who underwent pyloromyotomy for congenital hypertrophic pyloric stenosis over a 66-month period w...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2003.11.047

    authors： Yagmurlu A,Barnhart DC,Vernon A,Georgeson KE,Harmon CM

    更新日期：2004-03-01 00:00:00

  • Treatment of a case with right-sided diaphragmatic hernia associated with an abnormal vessel communication between a herniated liver and the right lung.

    abstract：:We herein report a rare case of a newborn girl with a right-sided congenital diaphragmatic hernia where a herniated liver had an abnormal vessel communication with the right lung. A surgical repair was initially attempted through a thoracic approach at 4 days of age where only a plication of the hernia capsule was per...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2005.12.032

    authors： Tanaka S,Kubota M,Yagi M,Okuyama N,Ohtaki M,Yamazaki S,Shirai Y,Hatakeyama K

    更新日期：2006-03-01 00:00:00

  • Routine MRI evaluation of low imperforate anus reveals unexpected high incidence of tethered spinal cord.

    abstract：BACKGROUND/PURPOSE:Previous clinical practice has included evaluation for the presence of tethered cord in those children who have imperforate anus with a high lesion. To define the incidence in children with low lesions, the authors reviewed their experience with a protocol employing routine magnetic resonance imaging...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1053/jpsu.2002.33817

    authors： Golonka NR,Haga LJ,Keating RP,Eichelberger MR,Gilbert JC,Hartman GE,Powell DM,Vezina G,Newman KD

    更新日期：2002-07-01 00:00:00

  • Is epidural anesthesia truly the best pain management strategy after minimally invasive pectus excavatum repair?

    abstract：OBJECTIVE:The repair of pectus excavatum with bar placement is associated with substantial postoperative pain. Optimal pain control strategy has not been addressed with level 1 or substantial level 2 evidence. Many institutions operate under the assumption that a thoracic epidural offers the best pain control for these...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2007.09.024

    authors： St Peter SD,Weesner KA,Sharp RJ,Sharp SW,Ostlie DJ,Holcomb GW 3rd

    更新日期：2008-01-01 00:00:00

  • Dysphagia because of unilateral internal jugular vein phlebectasia in an infant.

    abstract：:Phlebectasia affecting the internal jugular vein is a rare cause of a benign neck swelling in children. They are mostly asymptomatic and therefore managed conservatively. Ligation of the vein and excision is usually avoided owing to the worry of raised intracranial pressure. We report a case of a large right internal ...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2016.04.019

    authors： Sundaram J,Menon P,Thingnum SK,Rao KL

    更新日期：2016-07-01 00:00:00

  • Persistent ascites can be effectively treated by peritoneovenous shunts.

    abstract：AIM:The aim the study was to review our experience and determine the effectiveness of peritoneovenous shunts in the management of persistent ascites. METHODS:A retrospective review of all patients who had a peritoneovenous shunt inserted for persistent ascites in our institution over 32 years (1977-2010) was performed...

    journal_title：Journal of pediatric surgery

    pub_type： 杂志文章

    doi：10.1016/j.jpedsurg.2010.11.006

    authors： Rahman N,De Coppi P,Curry J,Drake D,Spitz L,Pierro A,Kiely E

    更新日期：2011-02-01 00:00:00