Time-resolved microspectrofluorometry and fluorescence imaging techniques: study of porphyrin-mediated cellular uptake of oligonucleotides.

Abstract:

:Time-resolved confocal microspectrofluorometry and fluorescence microscopy imaging were applied to monitor the cellular uptake of fluorescent-labeled oligonucleotides (ONs) delivered by a porphyrin molecule. The fate of porphyrin-ON complexes inside living cells has also been monitored. Due to intrinsic fluorescence of the porphyrin and sensitivity of its characteristics to microenvironment, multicomponent analysis of time-resolved fluorescence provides unique information about stability of the porphyrin-ON complexes, ON interactions with their target sequences, and ON and porphyrin distributions after delivery inside the cells. Time-resolved confocal microspectrofluorometry indeed delivers additional information compared with fluorescence confocal microscopy imaging widely employed to study ON uptake.

journal_name

Ann N Y Acad Sci

authors

Praus P,Kocisová E,Mojzes P,Stepánek J,Seksek O,Sureau F,Turpin PY

doi

10.1196/annals.1430.007

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

117-21

eissn

0077-8923

issn

1749-6632

pii

1130/1/117

journal_volume

1130

pub_type

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