Abstract:
:Time-resolved confocal microspectrofluorometry and fluorescence microscopy imaging were applied to monitor the cellular uptake of fluorescent-labeled oligonucleotides (ONs) delivered by a porphyrin molecule. The fate of porphyrin-ON complexes inside living cells has also been monitored. Due to intrinsic fluorescence of the porphyrin and sensitivity of its characteristics to microenvironment, multicomponent analysis of time-resolved fluorescence provides unique information about stability of the porphyrin-ON complexes, ON interactions with their target sequences, and ON and porphyrin distributions after delivery inside the cells. Time-resolved confocal microspectrofluorometry indeed delivers additional information compared with fluorescence confocal microscopy imaging widely employed to study ON uptake.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Praus P,Kocisová E,Mojzes P,Stepánek J,Seksek O,Sureau F,Turpin PYdoi
10.1196/annals.1430.007subject
Has Abstractpub_date
2008-01-01 00:00:00pages
117-21eissn
0077-8923issn
1749-6632pii
1130/1/117journal_volume
1130pub_type
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