Light microscopic visualisation of the presynaptic nerve terminal calyx in dissociated chick ciliary ganglion neurons.

Abstract:

:The pre- and postsynaptic elements of the calyx-type synapse in the chick ciliary ganglion were stained with Lucifer yellow in situ, and the structure of this synapse was examined after enzymatic dissociation of the ganglion. Back-staining of the ciliary nerve resulted in darkly stained neuronal cell bodies. Foreward-staining of the presynaptic oculomotor nerve did not stain the neurons, but instead resulted in a 'halo' of fluorescence around the cell bodies, corresponding to the large presynaptic calyxes. This study demonstrates the feasability of staining the presynaptic terminals in dissociated ciliary neurons and demonstrates the range of calyx structure at this physiologically interesting synapse.

journal_name

Brain Res

journal_title

Brain research

authors

Stanley EF

doi

10.1016/0006-8993(87)91309-6

subject

Has Abstract

pub_date

1987-09-22 00:00:00

pages

367-9

issue

1-2

eissn

0006-8993

issn

1872-6240

pii

0006-8993(87)91309-6

journal_volume

421

pub_type

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