Abstract:
BACKGROUND:The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs and the migration of dendritic cells into afferent lymphatic vessels. Antigen presentation, T cell recruitment, and expansion of regulatory cells are crucial events in establishing and controlling chronic allograft dysfunction. In this study, we report an important role for chemokine receptor CCR7 in the development of transplant arteriosclerosis. METHODS:Fully major histocompatibility complex-mismatched CBA (H2) donor aortas were transplanted into BALB/c-CCR7 (H2), BALB/c-CCR7 (H2), or BALB/c-CCR7 (H2) recipients. Grafts were analyzed by histology, morphometry, and immunofluorescence on day 30 after transplantation. Intragraft cytokine mRNA production was analyzed by realtime polymerase chain reaction on day 14 after transplantation. RESULTS:After implanting fully major histocompatibility complex-mismatched donor aortas into CCR7-deficient recipients, transplant arteriosclerosis was significantly elevated. CD4 depletion resulted in a reduction of intima proliferation in CCR7 recipients whereas CD8 depletion had no effect. Analysis of aortic grafts from CCR7 recipients revealed high numbers of infiltrating CD4, F4/80, and CD205 cells. Furthermore, intragraft cytokine production showed higher levels of interleukin-4, interleukin-12, and eotaxin mRNA expression, whereas significantly lower Foxp3 mRNA expression was observed in CCR7 recipients. CONCLUSION:These data suggest that although alloantigen presentation in secondary lymphoid organs is hampered in CCR7-deficient recipients, this process may take place within the allograft itself, leading to increased formation of transplant arteriosclerosis. The decrease in Foxp3 expression despite increased in CD4 T cell infiltration indicates a reduction in T regulatory cells possibly influencing the intensity of the graft rejection.
journal_name
Transplantationjournal_title
Transplantationauthors
Ensminger SM,Helm SN,Ohl L,Spriewald BM,Abele S,Wollin M,Wood KJ,Weyand M,Förster Rdoi
10.1097/TP.0b013e3181826a97subject
Has Abstractpub_date
2008-08-27 00:00:00pages
590-600issue
4eissn
0041-1337issn
1534-6080pii
00007890-200808270-00018journal_volume
86pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Since 1995, dual-kidney transplantation using organs from marginal donors has been used at our center to expand the organ donor pool and decrease the waiting time for deceased donor kidney transplantation. This approach has allowed for a shorter waiting period without compromising outcome in the early posttr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000130452.01521.b1
更新日期:2004-09-15 00:00:00
abstract:BACKGROUND:Some statins have been reported to suppress the immune system and increase the expression of bone morphogenetic protein-2 gene that plays a pivotal role in bone regeneration. METHODS:The effects of cerivastatin on skeletal reconstruction by vascularized bone allograft were investigated in a rat tibia-fibula...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000074992.49236.58
更新日期:2003-09-15 00:00:00
abstract::A cadaveric renal transplant was performed on a 63-year-old woman. The donor renal artery and vein were anastomosed to the recipient external iliac vessels using the vascular clipping system. These vascular anastomoses were performed with four stay sutures and several clips for each anastomosis, without a continuous v...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199610270-00024
更新日期:1996-10-27 00:00:00
abstract::A prospective randomized trial was performed comparing survival of cadaveric grafts allocated by HLA-A+B matching and HLA-DR matching. The two allocation methods resulted in very similar graft survivals. HLA-A matching had no significant effect on graft survival. HLA-B and HLA-DR matching were shown to have approximat...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/00007890-198407000-00010
更新日期:1984-07-01 00:00:00
abstract::A quantitative absorption-blocking assay was used to measure the levels of antibody in immune rabbit sera that react with the serologically defined HLA molecular complex on the cell surface. Quantitation was determined by comparing the number of xenoantibody-treated cells with the number of untreated control cells nee...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197807010-00009
更新日期:1978-07-01 00:00:00
abstract:BACKGROUND:Human herpesvirus-6 (HHV-6) is known to reactivate after renal transplantation and has been associated with several clinical manifestations. Risk factors for sustained viral replication, however, remain unclear. METHODS:Thirty consecutive kidney transplant patients were prospectively followed for HHV-6 repl...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182807ab7
更新日期:2013-03-27 00:00:00
abstract::Second clinical kidney grafts often survive longer than first grafts. Using a rat cardiac allograft model, we examined the conditions under which survival of a second graft can be longer than first graft survival. In the BUF-to-LEW combination, following rejection of the first transplant, second cardiac allografts fro...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199210000-00025
更新日期:1992-10-01 00:00:00
abstract::Mice of the B10 background that are class II I-E nonexpressing demonstrate a relative resistance to neonatal induction of tolerance of class I alloantigens from I-E-expressing B10 strains. The majority of these injected mice delete donor-responsive and "I-E-reactive" (V beta 11+) cells in the immediate postinoculation...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199312000-00035
更新日期:1993-12-01 00:00:00
abstract::A laparoscopic live-donor nephrectomy was performed on a 40-year-old man. The kidney was removed intact via a 9-cm infraumbilical midline incision. Warm ischemia was limited to less than 5 min. Immediately upon revascularization, the allograft produced urine. By the second postoperative day, the recipient's serum crea...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-11-15 00:00:00
abstract::Patients with steroid-resistant nephrotic syndrome and focal segmental glomerulosclerosis (FGS) who develop end-stage renal disease are at risk for recurrence of the disease following renal transplantation. Recurrence of the nephrotic syndrome in renal allografts of two children with primary FGS was successfully contr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198810000-00014
更新日期:1988-10-01 00:00:00
abstract::Experiments were performed on the quality of renal functional recovery after 24, 48, or 72-hr hypothermic storage preservation of canine kidneys in Euro-Collins solution (EC), Collins' solution C2, hypertonic citrate solutions (HC, HC-D2O), or our new flush solution 2 (F.2). Clearance tests (inulin, paraaminohippuric ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198502000-00003
更新日期:1985-02-01 00:00:00
abstract::With advances in microsurgery and molecular biology, the mouse model for organ transplantation has become increasingly popular. However, knowledge about these models is limited, as only a small number of centers have experience with murine models. In this study, we compared the rejection pattern after liver, kidney, h...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199611150-00016
更新日期:1996-11-15 00:00:00
abstract:OBJECTIVE:Intrahepatic infusion is the most common method of islet autotransplantation. Structural and functional changes within the liver may result from a number of factors, including embolization of the terminal branches of the portal vein, the effects of high insulin concentration on surrounding hepatocytes or resp...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31817348d6
更新日期:2008-06-27 00:00:00
abstract::Reduced-size liver transplantation in the rat has been useful in the study of hepatic regeneration. We describe a modified technique for partial liver transplantation in the rat using a 50% reduced-size graft. Male Lewis rats (RT1(1)), weighing 250 to 280 g, were used as donors and recipients. The harvested donor live...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199607270-00023
更新日期:1996-07-27 00:00:00
abstract::Endothelial cells, one of several in vivo host cells for cytomegalovirus (CMV), participate in solid organ allograft rejection in part through the expression of leukocyte adhesion molecules. The hypothesis that CMV infection alters the constitutive and induced expression of ELAM-1, ICAM-1, and VCAM-1 on infected human...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-12-27 00:00:00
abstract:BACKGROUND:Fatty livers are more prone to primary nonfunction after transplantation. It is known that cell injury is strongly associated with alterations in the content and composition of membrane lipids. We assumed that plasma membrane (PM) fluidity, which is the most important property of the membrane, differed betwe...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199901270-00002
更新日期:1999-01-27 00:00:00
abstract:BACKGROUND:Neighborhood poverty has been associated with worse outcomes after live donor kidney transplantation (LDKT), and prior work suggests that women with kidney disease may be more susceptible to the negative influence of poverty than men. As such, our goal was to examine whether poverty differentially affects wo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002654
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:The shortage of organs for liver transplantation has forced transplant centers to expand the donor pool by using donors traditionally labeled as marginal. One such example is liver transplantation using a donor with HELLP syndrome (hemolysis, elevated liver enzymes, and low platelets), a disorder of late pre...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000101510.01404.F0
更新日期:2004-01-15 00:00:00
abstract::Intestinal transplantation has become the treatment of choice for patients who are experiencing life-threatening complications of intestinal failure. Early attempts with intestinal transplantation were unsuccessful as a consequence of both technical and immunologic failures. The introduction of tacrolimus provided the...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000126931.23349.15
更新日期:2004-05-15 00:00:00
abstract::It was the objective of these experiments to study the time-related changes in the responsiveness of the cellular elements of the immune system following contact with single non-H-2 or multiple H-2 histocompatibility antigens. The reactivity of spleen cells from mice that received injections of spleen cells bearing H-...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198802000-00047
更新日期:1988-02-01 00:00:00
abstract::The effect of protein depletion on immune responsiveness was examined using a skin allograft model. Protein depletion was induced in adult Fischer (F344) male rats by the ad libitum provision of a 5% protein diet. Total serum protein, total body weight, total body nitrogen, and total body lipid were all markedly decre...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198009000-00012
更新日期:1980-09-01 00:00:00
abstract::The beneficial effect of deoxyspergualin (DSG, NKT-01) on lethal graft-versus-host disease in mice has been studied in a major histoincompatible donor-recipient combination. Suppression of the effector mechanisms responsible for the lethal outcome in this GVHD model is also noted. This study reveals: (1) DSG has a mar...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199103000-00032
更新日期:1991-03-01 00:00:00
abstract::Frozen sections of lymph nodes from normal mice were examined by immunofluorescence with anti-T lymphocyte and anti-Ig reagents and by autoradiography following [3H]TdR administration. The scattered T lymphocytes present among B lymphocytes of primary follicles were found to be mostly nonproliferating; the few prolife...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198111000-00018
更新日期:1981-11-01 00:00:00
abstract::To investigate the role of natural killer (NK) cells in the induction and pathogenesis of graft-versus-host (GVH) disease, +/beige (+/bg; normal NK cell activity) and beige/beige (bg/bg; deficient NK cell activity) parental C57BL/6 (B6) lymphoid cells were used to induce GVH reactions in either B6 X C3H/Hej +/bg (+/bg...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198708000-00017
更新日期:1987-08-01 00:00:00
abstract:BACKGROUND:The aim of our study was to examine, in a recent cohort of kidney transplant recipients who have received modern immunosuppressive therapy, the respective role of cold ischemia time (CIT) and delayed graft function (DGF) on acute rejection (AR) rates and long-term graft survival. METHODS:We retrospectively ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318169c29e
更新日期:2008-04-15 00:00:00
abstract::Surgical site infection (SSI) is an important cause of morbidity after orthotopic liver transplantation (OLT). Perioperative antibiotic prophylaxis is one of the main modifiable risk factors. We prospectively investigated the epidemiology, risk factors, and prognosis of SSI in a cohort of 167 OLT. Two different schedu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181735407
更新日期:2008-06-27 00:00:00
abstract:BACKGROUND:In France, the need for continuous monitoring of transplant center performance has recently become apparent. Cumulative sum (CUSUM) monitoring of transplantation is already been used to monitor transplant outcomes in the United Kingdom and in the United States. Because CUSUM monitoring can be applied by diff...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002591
更新日期:2019-09-01 00:00:00
abstract::Since June 1979, percutaneous transluminal angioplasty (PTA) has been the procedure of choice for renal transplant artery stenosis (RTAS) at the Hospital of the University of Pennsylvania. Of 241 renal allograft recipients, 17 (7%) when studied by arteriogram because of suspected RTAS proved to have significant stenos...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198212000-00005
更新日期:1982-12-01 00:00:00
abstract::The question has not been previously investigated as to whether host lymphocytes infiltrate vascularized organ allografts in indiscriminate fashion, or whether they are retained selectively because of specific antigenic recognition sites on their surfaces. By using a dual cardiac allograft model in LEW rats, we have e...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197809000-00011
更新日期:1978-09-01 00:00:00
abstract::A technique for orthotopic bone transplantation in mice has been developed. A section of recipient tibia was removed and replaced by a similar section from the donor animal. The graft was held in place by internal fixation. Bone healing was assessed clinically, histologically, radiologically, and by torsion testing. T...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00