Abstract:
:ABSTRACT Infection of soybean (Glycine max) plants inoculated with particles of Alfalfa mosaic virus (AlMV) isolate 425 at 12 days after germination was monitored throughout the life cycle of the plant (vegetative growth, flowering, seed formation, and seed maturation) by western blot analysis of tissue samples. At 8 to 10 days after inoculation, the upper uninoculated leaves showed symptoms of virus infection and accumulation of viral coat protein (CP). Virus CP was detectable in leaves, stem, roots, seedpods, and seed coat up to 45 days postinoculation (dpi), but only in the seedpod and seed coat at 65 dpi. No virus accumulation was detected in embryos and cotyledons at any time during infection, and no seed transmission of virus was observed. Soybean plants inoculated with recombinant AlMV passaged from upper uninoculated leaves of infected plants showed accumulation of full-length chimeric AlMV CP containing rabies antigen in systemically infected leaves and seed coat. These results suggest the potential usefulness of plants and plant viruses as vehicles for producing proteins of biomedical importance in a safe and inexpensive manner. Moreover, even the soybean seed coat, treated as waste tissue during conventional processing for oil and other products, may be utilized for the expression of value-added proteins.
journal_name
Phytopathologyjournal_title
Phytopathologyauthors
Fleysh N,Deka D,Drath M,Koprowski H,Yusibov Vdoi
10.1094/PHYTO.2001.91.10.941subject
Has Abstractpub_date
2001-10-01 00:00:00pages
941-7issue
10eissn
0031-949Xissn
1943-7684journal_volume
91pub_type
杂志文章相关文献
PHYTOPATHOLOGY文献大全abstract::ABSTRACT Striga hermonthica is a parasitic weed endemic to sub-Saharan Africa. It most commonly parasitizes sorghum, maize, pearl millet, and upland rice, lowering yields and affecting the welfare of over 100 million people, principally subsistence farmers. Cereal crops with complete resistance to this pathogen have n...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-95-1294
更新日期:2005-11-01 00:00:00
abstract::ABSTRACT Colletotrichum coccodes is a biocontrol agent of velvetleaf (Abutilon theophrasti), a noxious weed of corn and soybean. Metallothioneins (MTs) and basic region/leucine zipper motif (bZIP) are heavy-metal-binding proteins and transcription factors, respectively, that have been related to several plant processe...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-96-1116
更新日期:2006-10-01 00:00:00
abstract::ABSTRACT The prevalence of brown stem rot (caused by Phialophora gregata), Heterodera glycines, and Phytophthora sojae in the north central United States was investigated during the fall of 1995 and 1996. Soybean fields were randomly selected using an area-frame sampling design in collaboration with the National Agric...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1999.89.3.204
更新日期:1999-03-01 00:00:00
abstract::ABSTRACT Genetic variation within a worldwide collection of 208 isolates of Fu-sarium oxysporum f. sp. cubense, representing physiological races 1, 2, 3, and 4 and the 20 reported vegetative compatibility groups (VCGs), was analyzed using modified DNA amplification fingerprinting. Also characterized were 133 isolates ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.12.1283
更新日期:1998-12-01 00:00:00
abstract::Blast, caused by Pyricularia oryzae, has become an economically important disease in wheat in Brazil, but little effort has been devoted to understanding the wheat-P. oryzae interaction. This study was intended to determine the effects of P. oryzae infection on the photosynthetic process in wheat plants using a suscep...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-01-13-0024-R
更新日期:2014-01-01 00:00:00
abstract::ABSTRACT We determined that multiple and independent introductions of copper resistance genes have taken place for strains of Xanthomonas citri subsp. citri from Argentina and strains of X. alfalfae subsp. citrumelonis from Florida. This study compared the partial nucleotide sequences of principal copper resistance ge...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-06-12-0134-R
更新日期:2013-05-01 00:00:00
abstract::Late blight caused by Phytophthora infestans (Mont.) de Bary is a constraint to both potato and tomato crops in Nicaragua. The hypothesis that the Nicaraguan population of P. infestans is genotypically and phenotypically diverse and potentially subdivided based on host association was tested. A collection of isolates ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-11-0033
更新日期:2012-03-01 00:00:00
abstract::Fusarium lateritium is a globally distributed plant pathogen. It was recently reported as the causal agent of nut gray necrosis (NGN) on hazelnut. Isolate characterization within F. lateritium was undertaken to investigate how morphological and molecular diversity was associated with host and geographic origin. Morpho...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-10-0120
更新日期:2011-06-01 00:00:00
abstract::ABSTRACT Ten wild-type strains and two benomyl-resistant mutants of Talaromyces flavus were examined for their ability to secrete the cell wall-degrading enzymes chitinase, beta-1,3-glucanase, and cellulase, to parasitize sclerotia of Sclerotium rolfsii, to reduce bean stem rot caused by S. rolfsii, and to secrete ant...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.10.1054
更新日期:1997-10-01 00:00:00
abstract::Resin obtained from Pinus radiata and five monoterpene components of resin (limonene, α-pinene, β-pinene, camphene, and myrcene) were tested to determine their effects on mycelial growth and germination and survival of spores of Fusarium circinatum, the cause of pitch canker in pine, and F. temperatum, which is interf...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-14-0027-R
更新日期:2015-01-01 00:00:00
abstract::ABSTRACT A collection of race 1 and race 2 isolates of Fusarium oxysporum f. sp. lycopersici was screened for vegetative compatibility and characterized by random amplified polymorphic DNA (RAPD) analysis to establish the identity and genetic diversity of the isolates. Comparison of RAPD profiles revealed two main gro...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1999.89.2.156
更新日期:1999-02-01 00:00:00
abstract::Bean common mosaic virus (BCMV) exists as a complex of strains classified by reactions to resistance genes found in common bean (Phaseolus vulgaris); seven BCMV pathotypes have been distinguished thus far, numbered I to VII. Virus genetic determinants involved in pathogenicity interactions with resistance genes have n...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-08-13-0243-R
更新日期:2014-07-01 00:00:00
abstract::ABSTRACT During gel (gum) formation in angiosperm trees, fibrillar material accumulated in protective layers of xylem parenchyma cells before being secreted across half-bordered pit membranes into vessel elements. Immunogold labeling demonstrated that this fibrillar material was mainly composed of partially esterified...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.6.494
更新日期:1998-06-01 00:00:00
abstract::ABSTRACT Disease assessments and cytological investigations provided valuable information on the modes of action and efficacies of two prophylactic compounds, Milsana and benzothiadiazole (BTH), against powdery mildew development on long English cucumber. Milsana application significantly reduced disease incidence rel...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1999.89.9.728
更新日期:1999-09-01 00:00:00
abstract::ABSTRACT Plasmopara viticola populations collected from three islands in the Ionian Sea-an arm of the Mediterranean Sea to the west of Greece-were analyzed with microsatellite molecular markers in order to investigate the pathogen population structure. Downy mildew populations from mainland regions previously studied ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-96-0501
更新日期:2006-05-01 00:00:00
abstract::Dark pigmented fungi of the Gaeumannomyces-Phialophora complex were isolated from the roots of wheat grown in fields in eastern Washington State. These fungi were identified as Phialophora spp. on the basis of morphological and genetic characteristics. The isolates produced lobed hyphopodia on wheat coleoptiles, phial...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-100-5-0404
更新日期:2010-05-01 00:00:00
abstract::ABSTRACT The ability of Dactylella oviparasitica and Fusarium oxysporum to suppress Heterodera schachtii numbers was examined in field microplots. Fungi were individually added to fumigated field soil that was seeded with sugar beet. Four weeks later, soils were infested with H. schachtii second-stage juveniles (J2). ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-96-0855
更新日期:2006-08-01 00:00:00
abstract::The most economically important plant pathogens in the genus Pseudoperonospora (family Peronosporaceae) are Pseudoperonospora cubensis and P. humuli, causal agents of downy mildew on cucurbits and hop, respectively. Recently, P. humuli was reduced to a taxonomic synonym of P. cubensis based on internal transcribed spa...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-10-10-0270
更新日期:2011-07-01 00:00:00
abstract::Citrus is an economically important fruit crop that is severely afflicted by Asiatic citrus bacterial canker (CBC), a disease caused by the phytopathogen Xanthomonas citri subsp. citri (X. citri). To gain insight into the molecular epidemiology of CBC, 42 Xanthomonas isolates were collected from a range of Citrus spp....
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-11-12-0287-R
更新日期:2013-06-01 00:00:00
abstract::ND2710 is a hard red spring wheat line with a very high level of resistance to Fusarium head blight (FHB). It was selected from the progeny of a cross between ND2603 (an advanced breeding line derived from the Sumai 3/Wheaton cross) and Grandin (a spring wheat cultivar). The FHB resistance of ND2710 is presumably deri...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-12-17-0392-R
更新日期:2018-08-01 00:00:00
abstract::Although 'Candidatus Liberibacter asiaticus' (Las) is a major pathogen associated with citrus huanglongbing (HLB), some characteristics of transmission by the psyllid vector Diaphorina citri are not fully understood. We examined the latent period and persistence of transmission of Las by D. citri in a series of experi...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-16-0088-R
更新日期:2017-03-01 00:00:00
abstract::Pine wilt disease, caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, has become of worldwide quarantine concern in recent years. Here, we disclosed the surface coat (SC) proteins of the PWN which are thought to be one of the key components in pine wilt development. This is the first report that focus...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-10-0109
更新日期:2010-12-01 00:00:00
abstract::A growth assay in lemon juice and polymerase chain reaction amplifications using newly designed species-specific primers from endopolygalacturonase and β-tubulin genes rapidly differentiated isolates of the morphologically similar fruit sour rot pathogens Galactomyces citriaurantii and G. geotrichum. Isolates of both ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-05-11-0156
更新日期:2012-05-01 00:00:00
abstract::ABSTRACT Wheat was assessed at four crop growth stages for take-all (Gaeumannomyces graminis var. tritici) in a series of field trials that studied the effects of five wheat management practices: sowing date, plant density, nitrogen fertilizer dose and form, and removal/burial of cereal straw. An equation expressing d...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.1.26
更新日期:1997-01-01 00:00:00
abstract::ABSTRACT Late blight (Phytophthora infestans), pink rot (Phytophthora erythroseptica), leak (Pythium ultimum), dry rot (Fusarium sambucinum), and soft rot (Erwinia carotovora subsp. carotovora and subsp. atroseptica) are particularly damaging diseases of stored potato tubers worldwide. In this study, we present a meth...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-96-1037
更新日期:2006-09-01 00:00:00
abstract::ABSTRACT Restriction fragment length polymorphisms (RFLPs) were used to characterize the genetic structures of three field populations of Phaeosphaeria nodorum from Texas, Oregon, and Switzerland. Data from seven nuclear RFLP loci were used to estimate gene diversity and genetic distances and to make indirect measures...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.3.353
更新日期:1997-03-01 00:00:00
abstract::ABSTRACT Laboratory studies were conducted on the characteristics of beet yellows closterovirus (BYV) transmission to sugar beets by the bean aphid, Aphis fabae. The relative transmission efficiency and the retention, acquisition, and inoculation thresholds were evaluated using clonal aphid colonies. Transmission effi...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.7.766
更新日期:1997-07-01 00:00:00
abstract::We used a real time polymerase chain reaction-based assay and visual disease assessment to evaluate the efficacies of Js399-19, tebuconazole, a mixture of tebuconazole and thiram, azoxystrobin, carbendazim, and thiram on the development of Fusarium head blight (FHB) and deoxynivalenol (DON) contamination and on the yi...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-99-1-0095
更新日期:2009-01-01 00:00:00
abstract::Fifty strains of Fusarium oxysporum, recovered from rhizosphere soil around native Gossypium species and found to be mildly virulent on cotton (Gossypium hirsutum), were used to assay the propensity for evolution of virulence using serial passage assays through cotton. Only one lineage A strain, 2613, successfully com...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-3-0296
更新日期:2008-03-01 00:00:00
abstract::ABSTRACT Lepidopteran cells (Spodoptera frugiperda) produced isometric virus-like particles (VLP) when infected with a recombinant baculovirus Ac61 that contained the Potato leafroll virus (PLRV) coat protein gene modified with an N-terminal histidine tag (P3-6H). Cells infected with AcFL, a recombinant baculovirus th...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2000.90.10.1153
更新日期:2000-10-01 00:00:00