Abstract：
:By ammonium-sulphate fractionation, phosphocellulose column chromatography, and isoelectric focusing, pyruvate kinase (EC 2.7.1.40) was purified from pig dental pulps to yield a homogeneous preparation with a single protein band of approx. 63,000 daltons upon SDS-polyacrylamide gel electrophoresis. As the molecular weight of the enzyme was estimated to be 250,000 by Sephadex G-200 gel filtration, a tetrameric structure is indicated. The isoelectric point of the purified enzyme was 8.0; the optimal pH, pH 7.2. At this pH, there was no substrate inhibition of the enzyme by either phosphoenolpyruvate (PEP) or ADP, and the relationship between reaction velocity and each substrate concentration was well-explained by the Michaelis-Menten equation. The Km values were 53 and 286 microM for PEP and ADP, respectively. The enzyme had different catalytic properties at pH 8.0: with 0.4 mM ADP, the kinetic behaviour gave a sigmoidal curve with respect to PEP and fructose-1,6-diphosphate acted as an allosteric activator. With 2.0 mM ADP, a hyperbolic curve resulted with PEP.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Kobayashi H,Ozawa K,Yamada Sdoi
10.1016/0003-9969(86)90078-6subject
Has Abstractpub_date
1986-01-01 00:00:00pages
559-63issue
9eissn
0003-9969issn
1879-1506journal_volume
31pub_type
杂志文章abstract：:The periodontal ligament (PDL) contains precursor cells for osteoblasts and cementoblasts. It has been shown that epidermal growth factor (EGF) inhibits dexamethasone-induced differentiation and up-regulates EGF-receptor (EGF-R) expression, whereas EGF-R is down-regulated in the course of differentiation. Thus it was ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(98)00079-x
更新日期：1998-12-01 00:00:00
abstract：:Small GTPase Rab27 has been implicated in the regulation of different types of membrane trafficking, including melanosome transport and various regulated secretion events. We have previously shown that Rab27 and its effectors, Slac2-c/MyRIP and Slp4-a/granuphilin-a, are involved in the control of isoproterenol (IPR)-i...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.12.007
更新日期：2009-04-01 00:00:00
abstract：:Cells isolated by enzymatic digestion of gland fragments were incubated in solutions with or without Ca2+ or Na+ and exposed to monensin (10 microM) or acetylcholine (1 microM). Effects on accumulation of 22Na and on cell Ca2+ (measured with fura-2) were compared. In Ca2(+)-containing medium, accumulation of 22Na was ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90182-a
更新日期：1990-01-01 00:00:00
abstract：:Four-five months after the induction of diabetes, salivary tissues of male Wistar rats were preserved by glutaraldehyde fixation or rapid freezing in dry-ice cooled hexane. Fixed tissues were either processed and embedded for light and electron microscopy, or frozen and, together with unfixed tissues, sectioned and st...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90021-x
更新日期：1986-01-01 00:00:00
abstract：:The purpose of the study was to test the hypothesis that both insoluble pure type I collagen from bovine Achilles tendon and dentine collagen in root dentine powder from human teeth required acid pretreatment for subsequent degradation by trypsin, a non-specific protease. Pure type I collagen or dentine powder was tre...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90022-1
更新日期：1994-10-01 00:00:00
abstract：:There is evidence to suggest that some reimplanted and autotransplanted teeth are reinnervated, but the time-course of reinnervation and the properties and source of the reinnervating axons are not known. This reinnervation has been investigated using electrophysiological techniques in 6 adult cats. In each cat, one l...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90172-3
更新日期：1983-01-01 00:00:00
abstract：:The surface zone of human deciduous and permanent enamel was examined following sectioning, polishing and etching. Three main types of surface were where prisms reached the surface, where the prisms were not discernible and those formed by a complex of both types. The aprismatic layers were measured and the percentage...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90147-9
更新日期：1982-01-01 00:00:00
abstract：:Both Sjögren's syndrome and therapeutic irradiation for head and neck cancer lead to irreversible damage of the parenchyma of the salivary glands. This report describes an attempt to grow miniature pig (minipig) parotid gland cells on artificial films and tubular scaffolds with the ultimate intention of developing bio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.10.001
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVE:Presently, a large number of individuals consider their companion animals as family members and have close contact with them in daily life. The purpose of the present study was to analyze the distribution of periodontopathic bacterial species in oral specimens taken from dogs and their owners. DESIGN:Dental ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.02.015
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:This study was aimed to investigate the effects of microRNA-212-5p (miR-212-5p) and myeloid differentiation factor 88 (Myd88) in Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS)-challenged human periodontal ligament cells (PDLCs). METHODS:The levels of miR-212-5p and Myd88 in PDLCs were determ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104831
更新日期：2020-10-01 00:00:00
abstract：AIM:To compare the craniocervical angles and distances between temporomandibular dysfunction (TMD) and free TMD subjects. CASUISTIC AND METHODS:The sample consisted of young adults, of both genders, with age ranging between 18 and 30 years. TMD diagnosis was based on the clinical criteria of the Research Diagnostic Cr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.06.002
更新日期：2010-09-01 00:00:00
abstract：:A large earthquake (8-9 on the Richter scale) and a series of aftershocks took place on 2 March 1985 in Santiago, Chile. The characteristics of over 22,000 births registered in three public hospitals in the same year were reviewed. A significant increase in the rate of facial clefts was found; 2.01 per 1000 births in ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)00146-3
更新日期：1995-01-01 00:00:00
abstract：:Advances in biotechnology have brought gene therapy to the forefront of medical research. The concept of transferring genes to tissues for clinical applications has been discussed nearly half a century, but the ability to manipulate genetic material via recombinant DNA technology has brought this goal to reality. The ...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2014.11.018
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVE:The aim of the present study is to investigate the involvement of immunocompetent cells, pro-inflammatory cytokines and HSP, to evaluate a change of periodontal ligament during the initial stage of orthodontic tooth movement. DESIGN:In the present study, we investigated the distributional density of immunoco...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.11.010
更新日期：2011-05-01 00:00:00
abstract：INTRODUCTION:Caries lesions in dental hard tissues autofluoresce when exposed to light of certain wavelengths, whereas sound tissues do not, and this can be used as an in vitro histological marker for dental caries. Detection of autofluorescence is the basis of KaVo DIAGNOdent™ technology, and provides objective feedba...
journal_title：Archives of oral biology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.archoralbio.2012.09.017
更新日期：2013-06-01 00:00:00
abstract：:Unitary discharges of periodontal mechanosensitive (PM) fibres were recorded from cat inferior alveolar nerve. More than half (66%) of the PM units were single-tooth ones sensitive to only one tooth. The majority (78%) responded sustainedly to stimulation of the mandibular canine tooth. The multitooth units responded ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90036-1
更新日期：1994-01-01 00:00:00
abstract：:Human whole saliva was collected at 4 degrees C into a protein inhibitor cocktail to prevent proteolytic degradation. Saliva was clarified by centrifugation and fractionated by immobilized metal affinity chromatography (IMAC) after charging the column with zinc ions. Proteins with an affinity for zinc were eluted from...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00035-6
更新日期：1997-12-01 00:00:00
abstract：OBJECTIVE:To determine if dentine at the enamel-dentine junction (EDJ) in man is more sensitive to hydrostatic pressure stimuli then deeper dentine. DESIGN:Cavities (1mm diam.) were cut at the tips of the buccal and lingual cusps of 8 premolars in 3 subjects (ages: 22-25 years). Both cavities were initially deepened t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.02.015
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVE:We investigated the residual antibiofilm effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks. DESIGN:Dentin samples were prepared (n=120), sterilized and pretreated for 1 or 4 weeks with the clinically used concentration of DAP (500mg/mL), low con...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.06.006
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:Connexins (Cxs) are important to control growth and cell differentiation of dental tissues. The aim of the present study was to assess the impact of chronic exposure to sodium fluoride (NaF) on Cxs expression and alkaline phosphatase (ALP) activity in dental pulp, and on morphometric parameters of adult rat m...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.11.003
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:To clarify the fluoride/mineral kinetics in an oral biofilm following concurrent application of fluoride and other mineral ions released from experimental toothpaste containing S-PRG filler using depth-specific analysis. STUDY DESIGN:Twenty subjects wore in situ plaque-generating devices, comprised of a pair...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104777
更新日期：2020-09-01 00:00:00
abstract：:Autoantibodies directed against nucleic acid and protein complexes present in cell nuclei characterize autoimmune diseases and are employed in diagnosis. The mechanisms by which these autoantigens escape immunological tolerance are largely unknown, but a number of recent observations suggest that modified self-protein...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00025-0
更新日期：2002-06-01 00:00:00
abstract：OBJECTIVES:Radiographic dental age estimation methods are viable in the living and deceased. One such method [Kvaal et al. Forensic Sci Int 1995;74:175-85] quantified secondary dentinal deposition indirectly through measurements of tooth and pulp lengths and widths. The method is untested on non-European populations an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.08.020
更新日期：2012-03-01 00:00:00
abstract：:The inability of fibrocartilage, specifically the temporomandibular joint (TMJ) disc, to regenerate and remodel following injury presents a unique problem for clinicians. Tissue engineering then offers a potential regenerative therapy. In vitro testing provides a valuable screening tool for potential tissue engineered...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2017.07.013
更新日期：2017-11-01 00:00:00
abstract：:Biomechanical loads were varied by feeding diets of different consistencies. One group was fed hard diet for eight weeks, a second soft diet for eight weeks, and a third soft diet for four weeks followed by four weeks of hard diet. In all groups, alkaline-phosphatase activity was localized primarily in cells and extra...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90043-4
更新日期：1987-01-01 00:00:00
abstract：:The aim of this project was to develop an analytical procedure with the required level of sensitivity for the determination of glucose concentrations in small volumes of unstimulated fasting whole saliva. The technique involves high-performance ion-exchange chromatography at high pH and pulsed amperometric detection. ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00121-2
更新日期：1996-02-01 00:00:00
abstract：OBJECTIVE:Low dose propranolol has previously been demonstrated to suppress bone remodelling. Therefore, its effect on orthodontic movement was tested. DESIGN:Rats were assigned as follows (n=5): animals with no orthodontic appliance (G1); the remaining groups were fitted with a Ni-Ti closed-coil spring ligated to the...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.06.006
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of toothpaste treatments on levels of oral volatile sulphur compounds (VSCs) measured by gas chromatography in two clinical studies. METHODS:These were blinded, randomised, controlled, crossover studies with 16 (study A) or 20 (study B) healthy volunteers between the ages of 19-54. Stu...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/S0003-9969(08)70005-0
更新日期：2008-04-01 00:00:00
abstract：:Cyclic AMP phosphodiesterase (PDE) isozymes were isolated and characterized from the soluble fraction of rat parotid gland. Four main peaks containing PDE activity were obtained by Q-Sepharose Fast Flow column chromatography. The four peaks were identified as PDEs I-IV by kinetic properties, molecular-weight analysis ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)00139-3
更新日期：1995-02-01 00:00:00
abstract：:Herpes simplex virus type 1 (HSV1) can be rapidly identified in saliva from patients with acute herpetic gingivostomatitis, by in vitro amplification using the polymerase chain reaction and specific primers. Amplification of DNA results in a product of 110 bp length corresponding to the region 1381-1490 bp of the HSV1...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90113-m
更新日期：1992-10-01 00:00:00
