Abstract:
:The reactivity of acute myeloid leukemia cells (AML) was determined in 29 patients using the fucose binding lectin Ulex europaeus agglutinin (UEA) as surface marker. We show a marked heterogeneity in the UEA-binding abilities of the cells in these patients as determined by fluorescence analysis of the blasts labeled with the UEA coupled to the fluorescent molecule FITC. The results suggest a correlation between the capability of AML blast cells to bind UEA and cytologic maturation, because in 1 of 10 M1, 3 of 8 M2, 6 of 8 M4, and 1 of 3 M5 cytology types UEA binding to the leukemic cells was apparent. In 13 cases, the cells gave rise to colonies in vitro. The amount of UEA binding to AML colony-forming cells (AML-CFU) was determined by cell sorting and subsequent colony culture of UEA-negative, intermediately positive, and highly fluorescent cells. AML-CFU from none of the four patients with M1 cytology were UEA positive, whereas they showed intense reactivity with the lectin in 1 of 4 cases with M2 cytology and in all 4 cases of M4. In these five cases with strongly UEA positive AML-CFU, the fluorescence distribution of the colony formers differed from that of the total leukemia population, indicating that AML-CFU represent a subpopulation of AML cells with specific UEA-binding properties. Normal bone marrow myeloid and multipotential colony-forming cells (CFU-GM, CFU-GEMM) showed low or no binding of UEA. UEA-FITC appears a useful reagent for membrane analysis of AML-CFU. In certain cases, UEA-FITC labeling may be applied to discriminate AML-CFU from normal hematopoietic progenitors.
journal_name
Bloodjournal_title
Bloodauthors
Delwel R,Touw I,Bot F,Löwenberg Bsubject
Has Abstractpub_date
1986-07-01 00:00:00pages
41-5issue
1eissn
0006-4971issn
1528-0020journal_volume
68pub_type
杂志文章相关文献
BLOOD文献大全abstract:UNLABELLED:Better methods to characterize normal human hematopoietic cells with short-term repopulating activity cells (STRCs) are needed to facilitate improving recovery rates in transplanted patients.We now show that 5-fold more human myeloid cells are produced in sublethally irradiated NOD/SCID-IL-2Receptor-γchain-n...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-09-456566
更新日期:2013-01-31 00:00:00
abstract::HS1 (hematopoietic cell-specific Lyn substrate-1) is a cytoskeletal interactor in the B-cell receptor (BCR) signaling pathway whose phosphorylation correlates with prognosis in Chronic Lymphocytic Leukemia (CLL). The differentially phosphorylated sites and the kinases that regulate HS1 activity in CLL remain poorly un...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-09-457119
更新日期:2013-03-21 00:00:00
abstract::Extracellular histones have been shown to play an important pathogenic role in many diseases, primarily through their cytotoxicity toward nucleated cells and their ability to promote platelet activation with resultant thrombosis and thrombocytopenia. In contrast, little is known about the effect of extracellular histo...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2017-06-790519
更新日期:2017-12-28 00:00:00
abstract::Relapse following remission induction chemotherapy remains a barrier to survival in approximately 20% of children suffering from acute lymphoblastic leukemia (ALL). To investigate the mechanism of relapse, 27 matched diagnosis and relapse ALL samples were analyzed for clonal populations using polymerase chain reaction...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-01-067785
更新日期:2007-07-15 00:00:00
abstract::Intravital microscopy was used to monitor leukocyte traffic across rat mesenteric postcapillary venules induced by the inactive terminal complement (C) complex (iTCC) topically applied to ileal mesentery. Leukocytes started rolling within 15 minutes from the administration of iTCC, and by 1 hour they adhered almost co...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.1.185
更新日期:2002-01-01 00:00:00
abstract::The treatment of multiple myeloma has evolved significantly over the last decades from primarily alkylator-based chemotherapeutic agents with minimal efficacy to the introduction of more effective agents including immune modulators and proteasome inhibitors, which have changed the landscape of therapy for this disease...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2016-05-636357
更新日期:2016-09-29 00:00:00
abstract::Airway dendritic cells (DCs) are held responsible for inducing sensitization to inhaled antigen, leading to eosinophilic airway inflammation, typical of asthma. However, less information is available about the role of these cells in ongoing inflammation. In a mouse model of asthma, sensitization to ovalbumin (OVA) was...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-03-0673
更新日期:2002-11-15 00:00:00
abstract::Human cyclin A1 is a newly cloned, tissue-specific cyclin that is prominently expressed in normal testis. In this study, we showed that cyclin A1 was highly expressed in a subset of leukemia samples from patients. The highest frequency of cyclin A1 overexpression was observed in acute myelocytic leukemias, especially ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-03-15 00:00:00
abstract::Overexpression of transcription factors runt-related transcription factor 1 (RUNX1) and nuclear factor, erythroid-derived 2 (NF-E2) was reported in granulocytes of patients with polycythemia vera and other myeloproliferative neoplasms (MPNs). Further, a transgenic mouse overexpressing the NF-E2 transgene was reported ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-10-534222
更新日期:2014-01-16 00:00:00
abstract::The importance of the second messengers calcium (Ca(2+)) and diacylglycerol (DAG) in platelet signal transduction was established more than 30 years ago. Whereas protein kinase C (PKC) family members were discovered as the targets of DAG, little is known about the molecular identity of the main Ca(2+) sensor(s). We he...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-04-218768
更新日期:2009-09-17 00:00:00
abstract::Human subjects maintain long-term immunologic memory against infective organisms but the mechanism is unclear. CD4+ T-helper memory (Thmem) cells are pivotal in controlling humoral and cellular responses, therefore their longevity and response to vaccination are critical for maintenance of protective immunity. To prob...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-08-3255
更新日期:2006-04-01 00:00:00
abstract::The alpha thalassemias are associated with a decrease in alpha chain synthesis. Hemoglobin H (HbH) disease is a moderately severe form of alpha thalassemia characterized by the production of 5%--20% of HbH, while alpha thalassemia trait is a milder form of alpha thalassemia. In two patients with HbH disease, the ratio...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract::Oncogenic forms of NRAS are frequently associated with hematologic malignancies and other cancers, making them important therapeutic targets. Inhibition of individual downstream effector molecules (eg, RAF kinase) have been complicated by the rapid development of resistance or activation of bypass pathways. For the pu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-12-615906
更新日期:2015-05-14 00:00:00
abstract::Neutrophilic-chronic myeloid leukemia (CML-N) is a rare myeloproliferative disorder that runs a much more benign course than chronic myeloid leukemia, and for which no specific underlying molecular lesion has been described so far. We have analyzed the genomic DNA by Southern blotting and the BCR/ABL hybrid gene trans...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::Seventy-one patients with acute myeloid leukemia (AML), most of them (63/71) considered ineligible for conventional allogeneic hematopoietic stem cell transplantation (HSCT), were enrolled into a phase 2 study on reduced-intensity myeloablative conditioning with fractionated 8-Gy total body irradiation (TBI) and fluda...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2005-04-1377
更新日期:2005-11-01 00:00:00
abstract::Iron-regulatory proteins (IRP1 and IRP2) are RNA-binding proteins that bind to stem-loop structures known as iron-responsive elements (IREs). IREs are located in the 5'- or 3'-untranslated regions (UTRs) of specific mRNAs that encode proteins involved in iron homeostasis. The binding of IRPs to 5' IREs represses trans...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::To identify molecularly defined subgroups in multiple myeloma, gene expression profiling was performed on purified CD138(+) plasma cells of 320 newly diagnosed myeloma patients included in the Dutch-Belgian/German HOVON-65/GMMG-HD4 trial. Hierarchical clustering identified 10 subgroups; 6 corresponded to clusters desc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-12-261032
更新日期:2010-10-07 00:00:00
abstract::In an attempt to improve outcomes for patients with acute myeloid leukemia (AML) after allogeneic hematopoietic cell transplantation (HCT), we conducted a phase 1/2 study in which targeted irradiation delivered by 131I-anti-CD45 antibody was combined with targeted busulfan (BU; area-under-curve, 600-900 ng/mL) and cyc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-06-2317
更新日期:2006-03-01 00:00:00
abstract::Platelet factor 4 (PF4) serves as a lineage-specific marker of megakaryocyte development. We previously identified two positively acting sequences in the human platelet factor 4 (hPF4) gene promoter that synergized to drive high-level luciferase reporter gene expression in vitro. Using portions of the hPF4 5'-flanking...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Recently, messenger RNAs in eukaryotes have shown to associate with antisense (AS) transcript partners that are often referred to as long noncoding RNAs (lncRNAs) whose function is largely unknown. Here, we have identified a natural AS transcript for tyrosine kinase containing immunoglobulin and epidermal growth facto...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-09-242180
更新日期:2010-01-07 00:00:00
abstract::Activation of inflammatory and procoagulant mechanisms is thought to contribute significantly to the initiation of restenosis, a common complication after balloon angioplasty of obstructed arteries. During this process, expression of tissue factor (TF) represents one of the major physiologic triggers of coagulation th...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-04-1148
更新日期:2004-03-01 00:00:00
abstract::To understand the regulatory mechanism of erythropoietin (EPO) receptor (EPOR) gene expression, the effect of EPO on the steady-state level of EPOR mRNA was examined using the human EPO-dependent cell line UT-7 as a model system. We found that the treatment of UT-7 cells with EPO resulted in a transient decrease of th...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-02-15 00:00:00
abstract::We describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as FeLV-FAIDS) which induces fatal acquired immunodeficiency syndrome (AIDS) in 100% (25 of 25) of persistently viremic experimentally infe...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::Primitive chronic myeloid leukemia cells display a unique autocrine interleukin 3 (IL-3)/granulocyte-colony-stimluating factor (G-CSF) mechanism that may explain their abnormal proliferation and differentiation control. Here we show that BCR-ABL transduction of primitive Sca-1(+) lin(-) mouse bone marrow (BM) cells ca...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-05-1324
更新日期:2002-11-15 00:00:00
abstract::Chronic lymphocytic leukemia is characterized by relapse after treatment and chemotherapy resistance. Similarly, in other malignancies leukemia cells accumulate mutations during growth, forming heterogeneous cell populations that are subject to Darwinian selection and may respond differentially to treatment. There is ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-05-433540
更新日期:2012-11-15 00:00:00
abstract::Intrachromosomal amplification of chromosome 21 (iAMP21) defines a distinct subgroup of childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL) that has a dismal outcome when treated with standard therapy. For improved diagnosis and risk stratification, the initiating genetic events need to be elucidated. To...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-01-329961
更新日期:2011-06-23 00:00:00
abstract::Preliminary reports have suggested that survivors of childhood cancer and aplastic anemia who are infected with the hepatitis C virus (HCV) have a low risk for progression to significant liver disease. Among our surviving patients who were transfused between 1961 and March 1992, 77 (6.6% of surviving patients tested t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-05-15 00:00:00
abstract::Fifty-one children with acute lymphoblastic leukemia (ALL) in second or subsequent remission after a first remission of at least 24 months underwent purged, autologous bone marrow transplantation (ABMT). Bone marrow was harvested in remission and purged in vitro with monoclonal antibodies specific for leukemia-associa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-03-15 00:00:00
abstract::Crystallographic structures indicate that gamma-chain residue Asn308 participates in D:D interactions and indeed substitutions of gammaAsn308 with lysine or isoleucine have been identified in dysfibrinogens with impaired polymerization. To probe the role of Asn308 in polymerization, we synthesized 3 variant fibrinogen...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-12-4296
更新日期:2004-06-01 00:00:00
abstract::We have recently demonstrated that IgD(hi) B cells can occupy an extravascular perisinusoidal niche in the bone marrow in addition to the well-established follicular niche in conventional secondary lymphoid organs. The spleen has long been considered to be the site at which newly formed B lymphocytes mature into IgD(h...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-05-021089
更新日期:2007-03-15 00:00:00