Abstract:
:The mapping and characterization of the vasculature using proteomics offers the opportunity to better understand the steps and molecular mechanisms involved in vascular development, and angiogenesis in particular. Proteomics has many key advantages over genomics, especially in directly determining protein expression. Such an approach offers researchers the opportunity to discover the proteins that make up the vasculature in an a priori manner facilitating the generation of hypothesis which can subsequently be validated by other methods. This chapter focuses on proteomic principles and methods, with a particular focus on their applications to characterizing the vascular endothelium (both tumor and normal), as it exists in vivo.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Griffin NM,Schnitzer JEdoi
10.1016/S0076-6879(08)03008-5subject
Has Abstractpub_date
2008-01-01 00:00:00pages
177-208eissn
0076-6879issn
1557-7988pii
S0076-6879(08)03008-5journal_volume
445pub_type
杂志文章abstract::The ability to obtain large amounts of genomic sequence for organisms and high throughput technology has led to a change in the thrust of research at the level of chromosomes in animals. In the past chromosomal analysis of animals was focused on gross changes such as inversions, translocations, and deletions for both ...
journal_title:Methods in enzymology
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doi:10.1016/s0076-6879(00)05507-5
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abstract::Formation of 5-methyluridine (ribothymidine) at position 54 of the T-psi loop of tRNA is catalyzed by site-specific tRNA methyltransferases (tRNA[uracil-54,C5]-MTases). In eukaryotes and many bacteria, the methyl donor for this reaction is generally S-adenosyl-L-methionine (S-AdoMet). However, in other bacteria, like ...
journal_title:Methods in enzymology
pub_type: 杂志文章,评审
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journal_title:Methods in enzymology
pub_type: 杂志文章,评审
doi:10.1016/bs.mie.2014.10.009
更新日期:2015-01-01 00:00:00
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journal_title:Methods in enzymology
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更新日期:2009-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章,评审
doi:10.1016/S0076-6879(10)80009-6
更新日期:2010-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/B978-0-12-420037-1.00008-7
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journal_title:Methods in enzymology
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/B978-0-12-405881-1.00015-X
更新日期:2013-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/s0076-6879(02)59182-5
更新日期:2002-01-01 00:00:00
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更新日期:1994-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2019.04.018
更新日期:2019-01-01 00:00:00
abstract::The RNA degradosome is a bacterial protein machine devoted to RNA degradation and processing. In Escherichia coli, it is typically composed of the endoribonuclease RNase E, which also serves as a scaffold for the other components: the exoribonuclease PNPase, the RNA helicase RhlB, and enolase. The variable presence of...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(08)02206-4
更新日期:2008-01-01 00:00:00
abstract::Complex I is the major entry point in the bacterial and mitochondrial respiratory chain. Structural knowledge of the enzyme is still limited because of its large size and complicated architecture. Only the structure of the hydrophilic domain of a bacterial Complex I has been solved to high resolution by X-ray crystall...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(08)04401-7
更新日期:2009-01-01 00:00:00
abstract::Recent studies using sulfotransferase-deficient mice have revealed various physiological functions of sulfated glycans. Studies using gene-targeted mice deficient in both N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST)-1 and GlcNAc6ST-2 showed that these sulfotransferases play critical roles in lymphocyte homing....
journal_title:Methods in enzymology
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pub_type: 杂志文章
doi:10.1016/bs.mie.2019.10.015
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journal_title:Methods in enzymology
pub_type: 杂志文章
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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