Toward prevention of cowdriosis.

Abstract:

:Mass production of Ehrlichia ruminantium variants from different regions of sub-Saharan Africa is one of the difficulties that must be overcome in producing a heartwater vaccine. Vaccine productivity can be limited by endogenous induction of interferon (IFN), which inhibits the propagation of Ehrlichia ruminantium (ER) in cell culture. Different kinds of endothelial cells, in which ER multiply efficiently, could be grown in a scalable way in VueLife Teflon bags on Cytodex 3 microcarriers where bead-to-bead transfer of cells occurs. The digital holographic microscope designed at the Université Libre de Bruxelles allows detection of the most appropriate time to harvest intracellular microorganisms for vaccine production.

journal_name

Ann N Y Acad Sci

authors

Pedregal A,de Sousa DR,Nguyen HN,das Neves EA,Lowagie S,Marique T,Kagye N,Guerra IT,Kamba Y,Totte P,Vachiéry N,Lefrançois T,Martinez D,Yourassowsky C,Callens N,Monnom O,Dubois F,Wérenne J

doi

10.1196/annals.1428.091

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

286-91

eissn

0077-8923

issn

1749-6632

pii

NYAS1149091

journal_volume

1149

pub_type

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