Abstract:
:The binding of 1-anilino-8-naphthalenesulfonate (ANS) to bovine serum albumin (BSA), human serum albumin (HSA), and human plasma has been studied by potentiometric titration utilizing a laboratory constructed ion selective electrode (ISE) of ANS. Three classes of ANS binding sites were found on BSA, HSA, and plasma at 25 and 37 degrees C. Computer analysis of the data resulted in estimates for the association constants, number of binding sites (HSA, BSA), and binding capacity of each class. The association constants for the first class of binding sites at 25 degrees C were found to be 7.53 (+/- 0.59) x 10(5), 2.70 (+/- 0.20) x 10(5), and 2.64 (+/- 0.26) x 10(5) M-1 for BSA, HSA, and plasma, respectively. Lower values for the association constants of all binding classes were estimated at the higher temperature (37 degrees C). The binding capacity for ANS decreased in the order BSA, plasma, HSA.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Valsami GN,Macheras PE,Koupparis MAdoi
10.1023/a:1015855613083subject
Has Abstractpub_date
1991-07-01 00:00:00pages
888-92issue
7eissn
0724-8741issn
1573-904Xjournal_volume
8pub_type
杂志文章abstract:PURPOSE:To evaluate the potential of ascorbate as a novel ligand in the preparation of pharmaceutical nanocarriers with enhanced tumor-cell specific binding and cytotoxicity. METHODS:Palmitoyl ascorbate was incorporated into liposomes at varying concentrations. A stable formulation was selected based on size and zeta ...
journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
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更新日期:2017-02-01 00:00:00
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更新日期:2003-12-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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更新日期:2014-07-01 00:00:00
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更新日期:2012-07-01 00:00:00
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更新日期:2012-05-01 00:00:00
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更新日期:2019-06-03 00:00:00
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更新日期:2015-09-01 00:00:00
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更新日期:2020-09-28 00:00:00
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journal_title:Pharmaceutical research
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更新日期:1998-02-01 00:00:00
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更新日期:2011-07-01 00:00:00
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更新日期:2015-06-01 00:00:00
abstract::A colorless, parallelepiped crystal of methyl (2R,3S)-N-benzoyl-3-phenylisoserinate belonging to the space group P2(1) with a = 5.414(4), b = 7.813(1), c = 17.802(7) A, beta = 90.87(4) degrees, Z = 2, V = 752.9 A3, Dcalc = 1.32 g cm-3, and mu calc = 1.02 cm-1 was selected and the structure solved using direct methods....
journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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更新日期:2001-01-01 00:00:00