Role of viral suppressors governing asymmetric synergism between tomato-infecting begomoviruses.

Abstract:

:Mixed viral infections are common in fields and frequently exacerbate disease severity via synergistic interactions among individual viral genomic components leading to major crop loss. Two predominant species of tomato-infecting begomoviruses, Tomato leaf curl New Delhi virus (ToLCNDV) and Tomato leaf curl Gujarat virus (ToLCGuV), are known to cause severe leaf curl disease of tomato in India. Previously, we have demonstrated asymmetric synergism between these two distinct begomovirus species during mixed infection in solanaceous hosts. In the present study, we have identified the underlying proteins that positively regulate asymmetric synergism and their effect on plant defense machinery. During co-infection, the AC2 and AV2 of ToLCGuV enhanced ToLCNDV DNA accumulation in Nicotiana benthamiana as well as in their natural host, tomato. Furthermore, we found that AC2 and AV2 of ToLCNDV and AV2 of ToLCGuV play a critical role in suppression of post transcriptional gene silencing (PTGS) machinery. Taken together, AC2 and AV2 encoded proteins of ToLCGuV are the crucial viral factors promoting asymmetric synergism with ToLCNDV. KEY POINTS: • Begomoviral suppressors play vital roles in viral synergism. • AC2 and AV2 of ToLCGuV asymmetrically enhance ToLCNDV accumulation. • AC2 and AV2 of ToLCNDV and ToLCGuV AV2 are major PTGS suppressors.

authors

Basu S,Singh AK,Singh D,Sahu SK,Chakraborty S

doi

10.1007/s00253-020-11070-4

subject

Has Abstract

pub_date

2021-01-08 00:00:00

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-020-11070-4

pub_type

杂志文章
  • Bacterial synthesis of D-amino acids.

    abstract::Recent work has shed light on the abundance and diversity of D-amino acids in bacterial extracellular/periplasmic molecules, bacterial cell culture, and bacteria-rich environments. Within the extracellular/periplasmic space, D-amino acids are necessary components of peptidoglycan, and disruption of their synthesis lea...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-014-5726-3

    authors: Radkov AD,Moe LA

    更新日期:2014-06-01 00:00:00

  • Role of nanotechnology in agriculture with special reference to management of insect pests.

    abstract::Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. The potential uses and benefits of nanotechnology are enormous. These include insect pests management through the formulations of n...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-012-3969-4

    authors: Rai M,Ingle A

    更新日期:2012-04-01 00:00:00

  • A multiomics approach to study the microbiome response to phytoplankton blooms.

    abstract::Phytoplankton blooms are predictable features of marine and freshwater habitats. Despite a good knowledge base of the environmental factors controlling blooms, complex interactions between the bacterial and archaeal communities and phytoplankton bloom taxa are only now emerging. Here, the current research on bacterial...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-017-8330-5

    authors: Song L

    更新日期:2017-06-01 00:00:00

  • Metabolic activity of Corynebacterium glutamicum grown on L: -lactic acid under stress.

    abstract::Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0436-0

    authors: Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

    更新日期:2006-10-01 00:00:00

  • Impact of protein uptake and degradation on recombinant protein secretion in yeast.

    abstract::Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5783-7

    authors: Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

    更新日期:2014-08-01 00:00:00

  • Both BAT1 and ARO8 are responsible for unpleasant odor generation in halo-tolerant yeast Zygosaccharomyces rouxii.

    abstract::Soy sauce yeast Zygosaccharomyces rouxii plays a central role in the production of flavor compounds in soy sauce, while the flor-forming strain spoils its quality by producing 2-methylpropanoic acid, 2-methylbutanoic acid, and 3-methylbutanoic acid, which have an unpleasant odor. To investigate the relationship betwee...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6673-3

    authors: Watanabe J,Uehara K,Mogi Y

    更新日期:2015-09-01 00:00:00

  • Structural basis for a highly (S)-enantioselective reductase towards aliphatic ketones with only one carbon difference between side chain.

    abstract::Aliphatic ketones, such as 2-butanone and 3-hexanone, with only one carbon difference among side chains adjacent to the carbonyl carbon are difficult to be reduced enantioselectively. In this study, we utilized an acetophenone reductase from Geotrichum candidum NBRC 4597 (GcAPRD) to reduce challenging aliphatic ketone...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-10093-w

    authors: Koesoema AA,Sugiyama Y,Xu Z,Standley DM,Senda M,Senda T,Matsuda T

    更新日期:2019-12-01 00:00:00

  • Creation of cell surface-engineered yeast that display different fluorescent proteins in response to the glucose concentration.

    abstract::We have successfully created a novel yeast strain able to monitor changes in environmental conditions by displaying either green fluorescent protein (GFP) from Aequorea victoria or blue fluorescent protein (BFP), a variant of GFP, on its cell surface as a visible reporter. For the display of these fluorescent proteins...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530100767

    authors: Shibasaki S,Ueda M,Ye K,Shimizu K,Kamasawa N,Osumi M,Tanaka A

    更新日期:2001-11-01 00:00:00

  • Novel approaches and achievements in biosynthesis of functional isoprenoids in Escherichia coli.

    abstract::Isoprenoids, also referred to as terpenes, are the most diverse class of natural products appearing in a variety of natural sources, specifically in higher plants, and have a wide range of biological functions. This review describes novel or recent approaches and achievements in pathway engineering of Escherichia coli...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-009-2166-6

    authors: Harada H,Misawa N

    更新日期:2009-10-01 00:00:00

  • A novel synthetic fluoro-containing jasmonate derivative acts as a chemical inducing signal for plant secondary metabolism.

    abstract::A novel fluoro-containing jasmonate derivative was chemically synthesized and evaluated as a potential elicitor with respect to the induction of plant defense responses and the biosynthesis of plant secondary metabolites. A bioactive taxuyunnanine C (Tc)-producing cell line of Taxus chinensis was taken as a model plan...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1835-8

    authors: Qian ZG,Zhao ZJ,Xu Y,Qian X,Zhong JJ

    更新日期:2005-07-01 00:00:00

  • Changing glycine 21 for glutamic acid in the beta-subunit of penicillin G acylase from Kluyvera citrophila prevents protein maturation.

    abstract::Active penicillin acylase from Kluyvera citrophila strain ATCC 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. Using the chemical mutagen hydroxylamine we have treated plasmid pYKD59 containing the active penicillin acylase gene (pga) from K. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00183245

    authors: Prieto I,Rodríguez MC,Márquez G,Pérez-Aranda A,Barbero JL

    更新日期:1992-02-01 00:00:00

  • Lipids as renewable resources: current state of chemical and biotechnological conversion and diversification.

    abstract::Oils and fats are the most important renewable raw materials of the chemical industry. They make available fatty acids in such purity that they may be used for chemical conversions and for the synthesis of chemically pure compounds. Oleic acid (1) from "new sunflower," linoleic acid (2) from soybean, linolenic acid (3...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-006-0335-4

    authors: Metzger JO,Bornscheuer U

    更新日期:2006-06-01 00:00:00

  • Modeling of exo-inulinase biosynthesis by Kluyveromyces marxianus in fed-batch mode: correlating production kinetics and metabolic heat fluxes.

    abstract::A metabolic heat-based model was used for estimating the growth of Kluyveromyces marxianus, and the modified Luedeking-Piret kinetic model was used for describing the inulinase production kinetics. For the first time, a relationship was developed to relate inulinase production kinetics directly to metabolic heat gener...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7971-0

    authors: Santharam L,Samuthirapandi AB,Easwaran SN,Mahadevan S

    更新日期:2017-03-01 00:00:00

  • Back to the past: "find the guilty bug-microorganisms involved in the biodeterioration of archeological and historical artifacts".

    abstract::Microbial deterioration accounts for a significant percentage of the degradation processes that occur on archeological/historical objects and artworks, and identifying the causative agents of such a phenomenon should therefore be a priority, in consideration of the need to conserve these important cultural heritage it...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-9113-3

    authors: Mazzoli R,Giuffrida MG,Pessione E

    更新日期:2018-08-01 00:00:00

  • Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana.

    abstract::A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothri...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0447-x

    authors: Ying SH,Feng MG

    更新日期:2006-08-01 00:00:00

  • The redox-sensing transcriptional repressor Rex is important for regulating the products distribution in Thermoanaerobacterium aotearoense SCUT27.

    abstract::The redox-sensing transcriptional repressor Rex (Rex) displayed diverse functions in different microbial species. Nowadays, only part function of rex has been verified in vitro and alcohol dehydrogenase gene (adhE) as the target of Rex has been widely reported. In this study, rex was knocked out in Thermoanaerobacteri...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10554-7

    authors: Qu C,Chen L,Li Y,Fu H,Wang J

    更新日期:2020-06-01 00:00:00

  • Humic acid effect on pyrene degradation: finding an optimal range for pyrene solubility and mineralization enhancement.

    abstract::The addition of humic acid (HA) to polycyclic aromatic hydrocarbon (PAH) contaminated systems has been shown to enhance, inhibit, or have no effect on the biodegradation of these PAHs. In this study, the surfactant effects of Elliott soil HA (ESHA) at two pH values were tested. At pH 7.0, ESHA did not behave as a surf...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0769-8

    authors: Liang Y,Britt DW,McLean JE,Sorensen DL,Sims RC

    更新日期:2007-04-01 00:00:00

  • Structure and biotechnological applications of odorant-binding proteins.

    abstract::Odorant-binding proteins (OBPs) are small soluble polypeptides found in sensory organs of vertebrates and insects as well as in secretory glands and are dedicated to detection and release of chemical stimuli. OBPs of vertebrates belong to the family of lipocalin proteins, while those of insects are folded into α-helic...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-013-5383-y

    authors: Pelosi P,Mastrogiacomo R,Iovinella I,Tuccori E,Persaud KC

    更新日期:2014-01-01 00:00:00

  • Production of plant-derived polyphenols in microorganisms: current state and perspectives.

    abstract::Plants synthesize several thousand different polyphenols of which many have the potential to aid in preventing or treating cancer, cardiovascular, and neurodegenerative diseases. However, plants usually contain complex polyphenol mixtures impeding access to individual compounds in larger quantities. In contrast, funct...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-8747-5

    authors: Milke L,Aschenbrenner J,Marienhagen J,Kallscheuer N

    更新日期:2018-02-01 00:00:00

  • Extremophiles as a source of novel enzymes for industrial application.

    abstract::Extremophilic microorganisms are adapted to survive in ecological niches such as at high temperatures, extremes of pH, high salt concentrations and high pressure. These microorganisms produce unique biocatalysts that function under extreme conditions comparable to those prevailing in various industrial processes. Some...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s002530051456

    authors: Niehaus F,Bertoldo C,Kähler M,Antranikian G

    更新日期:1999-06-01 00:00:00

  • Natural and engineered promoters for gene expression in Lactobacillus species.

    abstract::Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in L...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-020-10426-0

    authors: Peirotén Á,Landete JM

    更新日期:2020-05-01 00:00:00

  • Psc-AFP from Psoralea corylifolia L. overexpressed in Pichia pastoris increases antimicrobial activity and enhances disease resistance of transgenic tobacco.

    abstract::Psc-AFP, isolated from the seeds of Psoralea corylifolia L., is an antimicrobial protein with trypsin inhibitor activity. Its encoding gene was cloned by 3'- rapid amplification of cDNA ends (RACE) combined with Y-shaped adaptor-dependent extension (YADE) method. The gene Psc-AFP encodes a protein of 203 amino acids w...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7768-1

    authors: Luo XM,Xie CJ,Wang,Wei YM,Cai J,Cheng SS,Yang X-,Sui A-

    更新日期:2017-02-01 00:00:00

  • A universal polyphosphate kinase: PPK2c of Ralstonia eutropha accepts purine and pyrimidine nucleotides including uridine diphosphate.

    abstract::Polyphosphosphate kinases (PPKs) catalyse the reversible transfer of the γ-phosphate group of a nucleoside-triphosphate to a growing chain of polyphosphate. Most known PPKs are specific for ATP, but some can also use GTP as a phosphate donor. In this study, we describe the properties of a PPK2-type PPK of the β-proteo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10706-9

    authors: Hildenbrand JC,Teleki A,Jendrossek D

    更新日期:2020-08-01 00:00:00

  • Catabolite-repressor-like protein regulates the expression of a gene under the control of the Escherichia coli lac promoter in the plant pathogen Xanthomonas campestris pv. campestris.

    abstract::Xanthomonas campestris pv. campestris, the causal agent of black-rot disease of cruciferous plants, and an important industrial microbe, was able to express the Escherichia coli beta-glucuronidase reporter gene (uidA) when fused to the E. coli lactose operon promoter on a wide-host-range plasmid vector. The gene fusio...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530050861

    authors: Soby SD,Daniels MJ

    更新日期:1996-12-01 00:00:00

  • Characteristics and N-terminal amino acid sequence of manganese peroxidase from solid substrate cultures of Agaricus bisporus.

    abstract::Extracellular manganese peroxidase (MnP) was purified from the compost extract of Agaricus bisporus using anion exchange chromatography, gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two forms (MnP1 and MnP2) were separated by isoelectric focusing and their isoelectric points...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530000509

    authors: Lankinen VP,Bonnen AM,Anton LH,Wood DA,Kalkkinen N,Hatakka A,Thurston CF

    更新日期:2001-03-01 00:00:00

  • Asparaginase inhibition of adhesion of type 1-fimbriated and P-fimbriated Escherichia coli to epithelial cell receptors.

    abstract::The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1710-7

    authors: Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

    更新日期:2004-11-01 00:00:00

  • Functional analysis of the dehydratase domains of a PUFA synthase from Thraustochytrium in Escherichia coli.

    abstract::Thraustochytrium sp. 26185, a unicellular marine protist, synthesizes docosahexaenoic acid, an omega-3 very long chain polyunsaturated fatty acid (VLC-PUFAs), by a polyunsaturated fatty acid (PUFA) synthase comprising three large subunits with multiple catalytic dehydratase (DH) domains critical for introducing double...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8635-4

    authors: Xie X,Meesapyodsuk D,Qiu X

    更新日期:2018-01-01 00:00:00

  • Use of Aspergillus overproducing mutants, cured for integrated plasmid, to overproduce heterologous proteins.

    abstract::Aspergillus niger var. awamori was previously transformed with a vector designed to express a fused glucoamylase-prochymosin gene and bearing the Neurospora crassa pyr4 gene as a selectable marker. Mutant strains that overproduced the glucoamylase-prochymosin fusion protein were derived from one of the transformants. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00164459

    authors: Ward M,Wilson LJ,Kodama KH

    更新日期:1993-08-01 00:00:00

  • Construction of first-generation lactococcal integrative cloning vectors.

    abstract::Using a randomly-cloned, HindIII-digested, chromosomal fragment from Lactococcus lactis subsp. lactis LM0230, first-generation lactococcal integrative cloning vectors were developed. Through dideoxy DNA sequence analysis, the cloned chromosomal DNA fragment was determined to be 1026 base pairs. Southern hybridization ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00170391

    authors: McIntyre DA,Harlander SK

    更新日期:1993-11-01 00:00:00

  • Screening of tetrachlorodibenzo- p-dioxin-degrading fungi capable of producing extracellular peroxidases under various conditions.

    abstract::Forty-six pulp-bleaching fungi were screened for production of key enzymes for conversion of polychlorinated dibenzo-p-dioxins--lignin peroxidase (LiP), manganese peroxidase (MnP), and manganese-independent peroxidase (MiP)--under various conditions that would allow their utilization in the environment. Of 38 MnP-prod...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1356-x

    authors: Manji S,Ishihara A

    更新日期:2004-01-01 00:00:00