Abstract:
OBJECTIVE:This study evaluates the antibacterial activity against mono and multispecies bacterial models and the cytotoxic effects of zinc oxide and copper nanoparticles(ZnO-NPs/Cu-NPs) in cell cultures of human gingival fibroblasts(HGFs). DESIGN:The antibacterial activities of ZnO-NPs and Cu-NPs against 4 bacteria species were tested according to their minimum inhibitory concentrations(MICs) and against mature multispecies anaerobic model by spectral confocal laser scanning microscopy. The viabilities and cytotoxic effects of ZnO-NPs and Cu-NPs to HGFs cell cultures were tested by MTT, LDH assays, production of ROS, and the activation of caspase-3. The results were analyzed using one-way ANOVA followed by Tukey tests, considering p < 0.05 as statistically significant. RESULTS:For all strains, MICs of ZnO-NPs and Cu-NPs were in the range of 78.3 μg/mL-3906 μg/mL and 125 μg/mL-625 ug/mL, respectively. In a multispecies model, a significant decrease in the total biomass volume(μ3) was observed in response to exposure to 125 μg/mL of each NPs for which there was bactericidal activity. Significant differences were found between the volumes of viable and nonviable biomass exposed to nanostructures with Cu-NPs compared to ZnO-NPs. Both NPs induced mitochondrial dose-dependent cytotoxicity, ZnO-NPs increases LDH release and intracellular ROS generation. Cu-NPs at a concentration of 50 μg/mL induced production of cleaved caspase-3, activating the apoptotic pathway early and at low doses. CONCLUSIONS:After 24 h, ZnO-NPs are biocompatible between 78-100 μg/mL and Cu-NPs below 50 μg/mL. Antibacterial activity in a monospecies model is strain dependent, and in a multispecies model was a lower doses after 10 min of exposure.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Vergara-Llanos D,Koning T,Pavicic MF,Bello-Toledo H,Díaz-Gómez A,Jaramillo A,Melendrez-Castro M,Ehrenfeld P,Sánchez-Sanhueza Gdoi
10.1016/j.archoralbio.2020.105031subject
Has Abstractpub_date
2021-02-01 00:00:00pages
105031eissn
0003-9969issn
1879-1506pii
S0003-9969(20)30409-Xjournal_volume
122pub_type
杂志文章abstract::Using a computerized technique the bone height and prevalence of approximal periodontal intrabony defects were assessed on posterior bite-wing radiographs from 151 young adults. The results were related to the buffering capacity and counts of Streptococcus mutans and lactobacilli in whole stimulated saliva. The mean d...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90161-3
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was to identify the anticancer effects and the mechanisms of action of shikonin and its analogue isobutyrylshikonin in oral squamous carcinoma cells. DESIGNS:The cytotoxic effects of isobutyrylshikonin and shikonin in Ca9-22 and SCC-25 cells were analyzed using 3-(4,5-dimethylth...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104774
更新日期:2020-08-01 00:00:00
abstract::Close association between nerve terminals and preodontoblasts, odontoblasts and predentine was observed during healing after pulpotomy. The nerve terminals frequently contained large numbers of synaptic vesicles. Terminals with many vesicles tended to be fewer in the predentine than in the odontoblastic layer. The dis...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00018-k
更新日期:1995-07-01 00:00:00
abstract::Sample groups of children (n=50) and adults (n=38) were selected from pools of 207 children, (11-13-year olds from two primary schools) and 94 adults (25-44-year olds from four governmental agencies) who were the subjects of an oral health survey among Tibetans living in Lhasa, Tibet Autonomous Region. Mean ages of th...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00201-7
更新日期:2003-02-01 00:00:00
abstract::The effects of interleukin-1 (IL-1) on the metabolism of two androgenic substrates, testosterone and 4-androstenedione, that can be converted to the potent anabolic metabolite 5 alpha-dihydrotestosterone (DHT) were studied. Duplicate incubations of confluent gingival fibroblasts were made in Eagle's minimum essential ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00058-w
更新日期:1995-10-01 00:00:00
abstract::Periodontal mechanosensitive (PM) primary afferent neurones were recorded from the rat trigeminal ganglion and their response to mechanical tooth stimulation studied. The majority (95%) of PM afferents were single-tooth units and most were sensitive to mechanical stimulation of the incisor. They had a sustained respon...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90167-8
更新日期:1994-05-01 00:00:00
abstract::To test the hypothesis that human tooth eruption requires a critical time period during which no force is applied to the erupting tooth, the eruption of a maxillary second premolar in the prefunctional stage was recorded during the afternoon or evening hours in eight children, using an optical device based on the prin...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00011-4
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVE:To test the hypothesis that experimental occlusal interferences increase the nerve growth factor (NGF) levels in periodontal tissues and cause an up-regulation of preprotachykinin-A (PPTA) mRNA. BACKGROUND:NGF is related to hyperalgesia and inflammation. PPTA mRNA, a primer of substance P, is a possible fact...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.08.007
更新日期:2010-12-01 00:00:00
abstract::Micelles represent macromolecular structures in saliva and the aim of this study was to identify salivary proteins that occur in these globular particles. Micelles were isolated from whole saliva (WS) collected from three individuals and analysed in different experiments. Samples were subjected to polyacrylamide gel e...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2003.11.007
更新日期:2004-05-01 00:00:00
abstract::A common finding in Swedish children is hypomineralization in first molars of unknown cause. Little is known about the chemical composition of the disturbed enamel. The aim here was to analyse the concentration gradients for F, Cl, Na, Mg, K and Sr in hypomineralized enamel from 17 permanent molars by means of seconda...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00113-8
更新日期:2001-03-01 00:00:00
abstract::The influence of indigenous Streptococcus mutans on naturally-occurring levels of IgA antibodies was studied in 42 lactating females. Breast milk, parotid and whole-saliva samples were collected and analysed by the ELISA method for IgA antibodies, reacting with antigens from Strep. mutans. All salivas and breast milk ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90149-x
更新日期:1987-01-01 00:00:00
abstract::The aim was to examine the nature of enamel dissolution in aqueous suspensions with a high solid/solution ratio and in a CO2-rich atmosphere. Before experimentation, a water-saturated mixture of 95% N2-5% CO2 was passed through the acid solutions for 24 hr. Samples of 2 g of powdered enamel were suspended in 7 ml of e...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(97)00043-5
更新日期:1997-09-01 00:00:00
abstract:OBJECTIVE:Enterococcus faecalis is a Gram-positive, facultative anaerobic bacterium that is associated with failed endodontic cases and nosocomial infections. E. faecalis can form biofilms, penetrate dentinal tubules and survive in root canals with scarce nutritional supplies. These properties can make E. faecalis resi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.07.001
更新日期:2016-11-01 00:00:00
abstract::The surface zone of human deciduous and permanent enamel was examined following sectioning, polishing and etching. Three main types of surface were where prisms reached the surface, where the prisms were not discernible and those formed by a complex of both types. The aprismatic layers were measured and the percentage...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90147-9
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:We investigated the residual antibiofilm effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks. DESIGN:Dentin samples were prepared (n=120), sterilized and pretreated for 1 or 4 weeks with the clinically used concentration of DAP (500mg/mL), low con...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.06.006
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVES:Dental caries is the most common chronic infectious disease in children. Streptococcus mutans, the main cariogenic bacterial species, produces persisters, nongrowing dormant variants of regular cells associated with chronicity of diseases. We hypothesized that the recurrent nature of caries, particularly wit...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.104601
更新日期:2020-02-01 00:00:00
abstract::New protocols for regenerative endodontic treatment along with the parallel development of tissue engineering technologies are changing traditional knowledge and treatment possibilities for young patients with pulp-affected permanent immature teeth. The regeneration and completion of apical root development in patholo...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2012.11.008
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVE:To compare the mineralisation density (MD), morphology and histology of alveolar bone and cementum amongst VDR +/+, VDR -/-, and VDR -/- groups supplemented with a diet TD 96348, containing 20% lactose, 2.0% calcium and 1.25% phosphorous. METHODS:Four groups of mice (6 mice/group) were identified by genotypi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.12.004
更新日期:2011-07-01 00:00:00
abstract::The pH effects of urea metabolism were studied in washed salivary-sediment bacteria from subjects that had up to 10-fold variation in oral ureolytic activity, and in dispersed artificial-mouth plaques. Adequate evaluation required analysis of the [OH-] as well as the pH curve. An initial constant rate of pH-change, la...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90132-4
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:This study aimed to examine and compare the anti-caries effects of citrus lemon oil (CLO) and limonene in rats. METHODS:The minimal inhibitory concentrations of CLO and limonene were measured using the disk diffusion method. The rats were infected with Streptococcus sobrinus and assigned into four groups: (1...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104851
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:This study aimed to evaluate the anti-caries effect of a fluoridated milk-based drink on a root caries model by assessing mineral loss and both biofilm microbial viability and acidogenicity under increasing concentrations of fluoride supplementation. DESIGN:Streptococcus mutans UA159 biofilms were grown on r...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104878
更新日期:2020-10-01 00:00:00
abstract::One dentine specimen was prepared from each of 90 bovine incisors. The samples were then evenly distributed among nine groups (A-I) and submitted to 10 alternating de- and re-mineralization cycles, including abrasion by tooth brushing. Each cycle started with a demineralization using the erosive soft drink Sprite Ligh...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(01)00039-5
更新日期:2001-09-01 00:00:00
abstract:OBJECTIVES:Tobacco use is one of the most important public health problems worldwide. It is also linked to impairment of normal immunologic surveillance and defence mechanism of polymorphonuclear leukocytes. Tobacco smoke and its components have been seen to affect the phagocytic ability and viability of polymorphonucl...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.09.018
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of a fluoride toothpaste containing nano-sized sodium trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm. DESIGN:This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.09.019
更新日期:2018-12-01 00:00:00
abstract::The mineralization levels of erupted buccal enamel from 24 deciduous teeth were compared to those of 28 permanent teeth. Sections were prepared in a defined plane using a lapping machine which gave plano-parallel sections. Mineralization levels were recorded by quantitative microradiography at 25 equivalent anatomical...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90130-1
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVES:The present in situ study investigated the effect of Inula viscosa tea on the pellicle's acid protective properties and on initial oral biofilm formation. DESIGN:Biofilm formation was performed on bovine enamel slabs on individual maxillary splints. Following 1min of pellicle formation, eight subjects rinse...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.07.006
更新日期:2016-11-01 00:00:00
abstract::Epidermal growth-factor receptor (EGF-r) has been identified on basilar cells of stratified squamous epithelia and skin adnexa in man. Recent studies have mapped EGF-r to various oral cells in animals; however, complete mapping of EGF-r in normal human oral mucosa has not been done. Normal tissues from eight sites in ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90079-2
更新日期:1993-09-01 00:00:00
abstract:OBJECTIVE:Secondary cartilage develops from osteochondral progenitor cells. Hypertrophic chondrocytes in secondary cartilage increase within a very short time and then ossify rapidly. In the present study, we investigated the sequential development process of osteochondral progenitor cells, and the morphology and size ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.12.027
更新日期:2018-05-01 00:00:00
abstract::This paper reports the development of a protocol allowing hybridization and detection of DNA fixed to nylon colony lifts from up to three species of bacteria simultaneously. Half ml samples of serial dilutions of pure cooked-meat broth (CMB) cultures of Capnocytophaga ochracea, Actinobacillus actinomycetemcomitans and...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90105-u
更新日期:1993-11-01 00:00:00
abstract:OBJECTIVE:Low dose propranolol has previously been demonstrated to suppress bone remodelling. Therefore, its effect on orthodontic movement was tested. DESIGN:Rats were assigned as follows (n=5): animals with no orthodontic appliance (G1); the remaining groups were fitted with a Ni-Ti closed-coil spring ligated to the...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.06.006
更新日期:2014-10-01 00:00:00