Cardiac structural changes and electrical remodeling in a thiamine-deficiency model in rats.

Abstract:

AIMS:Thiamine is an important cofactor present in many biochemical reactions, and its deprivation can lead to heart dysfunction. Little is known about the influence of thiamine deprivation on the electrophysiological behavior of the isolated heart cells and information about thiamine deficiency in heart morphology is controversial. Thus, we decided to investigate the major repolarizing conductances and their influence in the action potential (AP) waveform as well as the changes in the heart structure in a set of thiamine deficiency in rats. MAIN METHODS:Using the patch-clamp technique, we investigated inward (I(K1)) and outward K(+) currents (I(to)), T-type and L-type Ca(2+) currents and APs. To evaluate heart morphology we used hematoxylin and eosin in transversal heart sections. KEY FINDINGS:Thiamine deficiency caused a marked decrease in left ventricle thickness, cardiomyocyte number, cell length and width, and membrane capacitance. When evaluating I(to) we did not find difference in current amplitude; however an acceleration of I(to) inactivation was observed. I(K1) showed a reduction in the amplitude and slope conductance, which implicated a less negative resting membrane potential in cardiac myocytes isolated from thiamine-deficient rats. We did not find any difference in L-type Ca(2+) current density. T-type Ca(2+) current was not observed. In addition, we did not observe significant changes in AP repolarization. SIGNIFICANCE:Based on our study we can conclude that thiamine deficiency causes heart hypotrophy and not heart hypertrophy. Moreover, we provided evidence that there is no major electrical remodeling during thiamine deficiency, a feature of heart failure models.

journal_name

Life Sci

journal_title

Life sciences

authors

Roman-Campos D,Campos AC,Gioda CR,Campos PP,Medeiros MA,Cruz JS

doi

10.1016/j.lfs.2009.03.011

subject

Has Abstract

pub_date

2009-06-05 00:00:00

pages

817-24

issue

23-24

eissn

0024-3205

issn

1879-0631

pii

S0024-3205(09)00136-2

journal_volume

84

pub_type

杂志文章
  • Study on the metabolism of lyso-platelet activating factor (lyso-PAF) in human paranasal sinus mucosa. The cultured ciliated epithelium can convert lyso-PAF to PAF.

    abstract::The conversion of lyso-platelet activating factor (lyso-PAF) to PAF in cultured paranasal sinus mucosa obtained from normal human subjects was studied. The PAF concentration in the medium was determined after addition of lyso-PAF. PAF became detectable at 10 minutes after the addition of 10(-8)M lyso-PAF, and reached ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90483-j

    authors: Inoue H,Ganbo T,Hisamatsu K,Ishida H

    更新日期:1993-01-01 00:00:00

  • Protein phosphorylation in isolated human adipocytes-adrenergic control of the phosphorylation of hormone-sensitive lipase.

    abstract::The effect of adrenergic agents on protein phosphorylation in human adipocytes was examined. Freshly isolated human fat cells were incubated with 32PO4 in order to label intracellular ATP, then treated with a variety of adrenergic and other pharmacologic agents. Treatment with the beta-adrenergic agonist isoproterenol...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(90)90597-k

    authors: Smiley RM,Paul S,Browning MD,Leibel RL,Hirsch J

    更新日期:1990-01-01 00:00:00

  • Therapeutic interventions targeting the nitric oxide system: current and potential uses in obstetrics, bone disease and erectile dysfunction.

    abstract::Nitric oxide is involved in a countless number of physiological processes and is known to have cytoprotective as well as cytotoxic effects. Increased knowledge about the multifaceted role of nitric oxide in a variety of disease states has led to the design of multiple treatment strategies involving the nitric oxide sy...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2003.08.029

    authors: Grant MK,El-Fakahany EE

    更新日期:2004-02-20 00:00:00

  • Abolishment of TNBS-induced visceral hypersensitivity in mast cell deficient rats.

    abstract::Mucosal mast cells are implicated in visceral hypersensitivity associated with irritable bowel syndrome (IBS). In this study, we investigated the role of mast cells in the development of visceral hypersensitivity by using mast cell deficient (Ws/Ws) rats and their control (W+/W+). In W+/W+ rats, an injection of 2,4,6-...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2007.11.027

    authors: Ohashi K,Sato Y,Kawai M,Kurebayashi Y

    更新日期:2008-02-13 00:00:00

  • Does the sedative agent, JM-1232(-) cause QT prolongation with subsequent torsades de pointes?

    abstract:AIMS:JM-1232(-) ((-)-3-[2-(4-methyl-1-piperazinyl)-2-oxoethyl]-2-phenyl-3,5,6,7-tetrahydrocyclopenta[f] isoindole-1(2H)-one) is a novel isoindoline compound that acts on benzodiazepine receptors. Clinical trials are being conducted to assess its potential clinical use as an intravenous anesthetic or sedative agent. In ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2009.10.002

    authors: Kato R,Watanabe R,Miki H,Ijiri Y,Hayashi T,Tanaka K

    更新日期:2009-11-18 00:00:00

  • Nicotine administration to rats: methodological considerations.

    abstract::The effects of nicotine on normal physiological function are of increasing concern. Preliminary to studies on the effects of prenatal exposure to nicotine, we examined methods of administering nicotine to rats. Drinking water containing nicotine was not palatable to rats and was an unsatisfactory method in our hands, ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(87)90020-8

    authors: Murrin LC,Ferrer JR,Zeng WY,Haley NJ

    更新日期:1987-04-27 00:00:00

  • The protective effect of Ganoderma atrum polysaccharide against anoxia/reoxygenation injury in neonatal rat cardiomyocytes.

    abstract:AIMS:Oxidative stress has been largely implicated in the pathogenesis of anoxia/reoxygenation injury. Ganoderma atrum polysaccharide (PSG-1), the most abundant component extracted from the fruiting bodies of G. atrum, has been shown to possess potent antioxidant activity. In this study, we investigated whether PSG-1 at...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2009.09.001

    authors: Li WJ,Nie SP,Yan Y,Zhu SB,Xie MY

    更新日期:2009-10-21 00:00:00

  • Morphine treatment in vitro or in vivo decreases phagocytic functions of murine macrophages.

    abstract::Studies were performed to compare in vitro and in vivo effects of morphine on the phagocytic function of murine peritoneal macrophages. Macrophage monolayers were incubated with Candida albicans for 30 min in the absence of autologous serum. Morphine added in vitro was found to decrease both the phagocytic activity (p...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90122-j

    authors: Rojavin M,Szabo I,Bussiere JL,Rogers TJ,Adler MW,Eisenstein TK

    更新日期:1993-01-01 00:00:00

  • Inhibition of lipid peroxidation by botanical extracts of Ocimum sanctum: in vivo and in vitro studies.

    abstract::Ocimum sanctum, the Indian holy basil, has significant ability to scavenge highly reactive free radicals. Shade dried leaf powder of the plant was extracted with water and alcohol, and then fractionated with different solvents. Both extracts and their fractions have in vitro anti-lipidperoxidative activity at very low...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2004.05.036

    authors: Geetha RK,Vasudevan DM

    更新日期:2004-11-19 00:00:00

  • Impacts of icodextrin on integrin-mediated wound healing of peritoneal mesothelial cells.

    abstract:AIMS:Exposure to glucose and its metabolites in peritoneal dialysis fluid (PDF) results in structural alterations of the peritoneal membrane. Icodextrin-containing PDF eliminates glucose and reduces deterioration of peritoneal membrane function, but direct effects of icodextrin molecules on peritoneal mesothelial cells...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2012.04.036

    authors: Matsumoto M,Tamura M,Miyamoto T,Furuno Y,Kabashima N,Serino R,Shibata T,Kanegae K,Takeuchi M,Abe H,Okazaki M,Otsuji Y

    更新日期:2012-06-14 00:00:00

  • Tricyclic antidepressant drugs affect histamine receptors in human leukocytes.

    abstract::Tricyclic antidepressant drugs bind to histamine receptors in rodent and other mammalian tissues. However, a readily accessible, normal human tissue for studies of these drugs has not been available. We showed that freshly isolated human leukocytes can be used for this purpose. Following isolation by dextran sedimenta...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(84)90441-7

    authors: Sadava D,Wilmington K

    更新日期:1984-12-17 00:00:00

  • Temperature-sensitive high affinity [3H]tryptamine binding sites in rat brain.

    abstract::The influence of prior incubation on [3H]tryptamine binding was investigated in rat brain synaptic plasma membranes. A 55 min preincubation of the membranes at 37 degrees C induced an approx. 2.4-fold increase in the specific binding of [3H]ligand to the subsequently washed preparations and this phenomenon was quite t...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(86)90428-5

    authors: Karasawa A,Serikyaku S,Ishitani R

    更新日期:1986-04-07 00:00:00

  • Thermal inactivation of SARS COVID-2 virus: Are steam inhalations a potential treatment?

    abstract:BACKGROUND:The emergence of SARS-CoV-2 pandemic has upset health systems around the world and caused immeasurable losses and costs. Until a vaccine will become available, the recommended prevention measures remain physical distancing and enhanced hygiene. METHODS AND FINDINGS:The proteic structure external to the viru...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2020.118801

    authors: la Marca G,Barp J,Frenos S,Mugelli A,Galli L,Calistri E,Biasucci G,De Masi S,Guerrini R

    更新日期:2021-01-15 00:00:00

  • Benzodiazepine receptors on human blood platelets.

    abstract::Binding studies conducted on membrane preparation from human platelets using (3H) Ro5-4864 and (3H) diazepam showed specific and saturable binding. Scatchard analysis revealed a single class of binding sites with KD = 10.8 +/- 0.9 nM and Bmax = 775 +/- 105 fmol/mg protein for (3H) Ro5-4864 and KD = 10.5 +/- 1.1 nM and...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(84)90556-3

    authors: Moingeon P,Dessaux JJ,Fellous R,Alberici GF,Bidart JM,Motté P,Bohuon C

    更新日期:1984-11-12 00:00:00

  • Chromium attenuates hepatic damage in a rat model of chronic cholestasis.

    abstract:AIMS:Oxidative stress is involved in cholestasis-induced hepatic damage. Therefore, antioxidant therapy is a recommended therapeutic strategy. Studies have illustrated that chromium can enhance antioxidative capacity leading to a resolution of oxidative stress. The aim of this study was to assess whether chromium has p...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2009.02.003

    authors: Chen WY,Chen CJ,Liao JW,Mao FC

    更新日期:2009-04-24 00:00:00

  • Ectopic TWEAKR expression in type I fiber of stroke-prone spontaneously hypertensive rats is related to slow muscle-specific hypotrophy.

    abstract:AIMS:Stroke-prone spontaneously hypertensive rats (SHRSP) show significantly lower body weight than normotensive Wistar-Kyoto rats (WKY). Our hypotheses are as follows: weight loss of the skeletal muscle is related to hypertension-related diseases, and muscle hypotrophy is useful as a therapeutic target for hypertensio...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2019.116919

    authors: Inoue T,Hagiyama M,Maenishi O,Kimura M,Mizuguchi N,Mine Y,Kimura R,Chikugo T,Itoh T,Satou T,Ito A

    更新日期:2019-11-15 00:00:00

  • Structural analysis of ACE2 variant N720D demonstrates a higher binding affinity to TMPRSS2.

    abstract:AIMS:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel member of the betacoronaviruses family affecting the lower respiratory tract mainly through binding to angiotensin converting enzyme 2 (ACE2) via its S-protein. Genetic analysis of (ACE2) gene revealed several variants that have been suggested t...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2020.118219

    authors: Mohammad A,Marafie SK,Alshawaf E,Abu-Farha M,Abubaker J,Al-Mulla F

    更新日期:2020-10-15 00:00:00

  • A non-radioactive fluorescent method for measuring protein kinase C activity.

    abstract::The purpose of this study is to report the development of a non-radioactive fluorescent peptide assay for measuring protein kinase C activity (PKC). The assay is based on a glycogen synthase derived fluorescent peptide that is phosphorylated by PKC. Phosphorylation causes the peptide to migrate toward the anode while ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(95)02149-d

    authors: Isbell JC,Christian ST,Mashburn NA,Bell PD

    更新日期:1995-01-01 00:00:00

  • Synthetic human pancreatic growth hormone releasing factor (GRF) stimulates growth hormone secretion in the domestic fowl (Gallus domesticus).

    abstract::Synthetic human pancreatic Growth Hormone-Releasing Factor (hpGRF) elevated the plasma concentration of growth hormone (GH) in young and adult domestic fowl. This in vivo effect of hpGRF appeared to be largely similar for both the 32 amino-acid (hpGRF 1-32) or 40 amino-acid (hpGRF 1-40) polypeptide, although the effec...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(84)90083-3

    authors: Scanes CG,Carsia RV,Lauterio TJ,Huybrechts L,Rivier J,Vale W

    更新日期:1984-03-19 00:00:00

  • Selective serotonin reuptake inhibitors (SSRI) affect murine bone lineage cells.

    abstract:AIMS:Data suggest pharmacological treatment of depression with selective serotonin reuptake inhibitors (SSRI) may impair bone health. Our group has previously modeled compromised craniofacial healing after treatment with sertraline, a commonly prescribed SSRI, and hypothesized potential culprits: alterations in bone ce...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2020.117827

    authors: Durham E,Zhang Y,LaRue A,Bradshaw A,Cray J

    更新日期:2020-08-15 00:00:00

  • Inhibition of hepatic adenylate cyclase by NADH.

    abstract::Adenylate cyclase activity in isolated rat liver plasma membranes was inhibited by NADH in a concentration-dependent manner. Half-maximal inhibition of adenylate cyclase was observed at 120 microM concentration of NADH. The effect of NADH was specific since adenylate cyclase activity was not altered by NAD+, NADP+, NA...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(91)90177-d

    authors: Nair BG,Patel TB

    更新日期:1991-01-01 00:00:00

  • Central nervous system lipid alterations in rats with experimental allergic encephalomyelitis and its suppression by immunosuppressive drugs.

    abstract::Rats with experimental allergic encephalomyelitis (EAE) induced with myelin or spinal cord show decreases in the content of sulphatides and cerebrosides and increases in the level of esterified cholesterol in the CNS. In this work it is shown that brain sulphatide changes can be obtained by injection of mixtures conta...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(82)90600-2

    authors: Roth GA,Monferran CG,Maggio B,Cumar FA

    更新日期:1982-03-08 00:00:00

  • Changes in high affinity sodium independent gamma-aminobutyric acid binding in cerebral cortex and hippocampus of the rat following electroshock.

    abstract::Electroshock induced seizures in the rat enhanced high affinity specific Na+ independent binding of 3H-gamma aminobutyric acid (GABA) to frozen, Triton X-100 treated cerebral synaptic membranes 30 minutes after exposures to electroshock, although no change in 3H GABA binding was observed in similarly treated preparati...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(82)90756-1

    authors: Ross SM,Craig CR

    更新日期:1982-11-29 00:00:00

  • Bisphenol A: What lies beneath its induced diabetes and the epigenetic modulation?

    abstract::Nowadays, endocrine disrupting chemical pollution has become one of the major concerns due to the potential role of these chemicals in provoking endocrine disorders especially type 2 diabetes. As a widespread endocrine disrupting chemical, Bisphenol A, with modest estrogenic activity can exert its detrimental effects ...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2018.10.044

    authors: Rahmani S,Pour Khalili N,Khan F,Hassani S,Ghafour-Boroujerdi E,Abdollahi M

    更新日期:2018-12-01 00:00:00

  • S-8921, an ileal Na+/bile acid cotransporter inhibitor decreases serum cholesterol in hamsters.

    abstract::The ileal Na+/bile acid cotransporter (IBAT) maintains the reabsorption of bile acids from the intestine in the enterohepatic circulation of bile acids. In the present study, we showed that S-8921 could dose-dependently inhibit the uptake of [3H] taurocholate in the COS7 cell line which constitutively expresses hamste...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(97)00242-7

    authors: Hara S,Higaki J,Higashino K,Iwai M,Takasu N,Miyata K,Tonda K,Nagata K,Goh Y,Mizui T

    更新日期:1997-01-01 00:00:00

  • Restricted clinical efficacy of cyclosporin A on rat transient middle cerebral artery occlusion.

    abstract::The immunosuppressant cyclosporin A (CsA) has been shown to have neuroprotective action. The inhibition of both calcineurin activation and mitochondrial permeability transition pore (mtPTP) opening are considered the primary neuroprotective mechanisms of CsA. Here we have evaluated the effect of CsA on significantly r...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(02)02267-1

    authors: Matsumoto S,Isshiki A,Watanabe Y,Wieloch T

    更新日期:2002-12-20 00:00:00

  • Differential sensitivity to cocaine in spontaneously hypertensive and Wistar-Kyoto rats.

    abstract::Physiological, pharmacological and toxicological responses to two regimens of cocaine administration were compared between spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. An initial experiment examined renal excretory and hemodynamic function in response to an acute volume load in anesthetized SHR and WK...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(89)90254-3

    authors: Ishizuka Y,Rockhold RW,Kirchner K,Hoskins B,Ho IK

    更新日期:1989-01-01 00:00:00

  • Ablation of capsaicin-sensitive afferent nerves affects insulin response during an intravenous glucose tolerance test.

    abstract::We investigated the role of sensory nerves in glucose tolerance in conscious Wistar rats neonatally treated with neurotoxin capsaicin or vehicle. Intravenous glucose tolerance tests (IVGTT, 150, 300 and 450 mg in 30 min) were performed to measure glucose tolerance, and glucose, insulin and glucagon levels were measure...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2005.03.011

    authors: van de Wall EH,Gram DX,Strubbe JH,Scheurink AJ,Koolhaas JM

    更新日期:2005-07-29 00:00:00

  • Forkhead box transcription factors in embryonic heart development and congenital heart disease.

    abstract::Embryonic heart development is a very complicated process regulated precisely by a network composed of many genes and signaling pathways in time and space. Forkhead box (Fox, FOX) proteins are a family of transcription factors characterized by the presence of an evolutionary conserved "forkhead"or "winged-helix" DNA-b...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2015.12.001

    authors: Zhu H

    更新日期:2016-01-01 00:00:00

  • Inhibitory effects of pitavastatin on fibrogenic mediator production by human lung fibroblasts.

    abstract:AIMS:Idiopathic pulmonary fibrosis continues to be a devastating clinical disorder for which there are few therapeutic options, and the pathogenesis of this disease remains largely unknown. Statins are inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in cholesterol biosynthesis, and they have been reported...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2013.10.026

    authors: Oka H,Ishii H,Iwata A,Kushima H,Toba S,Hashinaga K,Umeki K,Tokimatsu I,Hiramatsu K,Kadota J

    更新日期:2013-12-18 00:00:00