Abstract：
OBJECTIVE:Inflammation and fibrosis progress of nucleus pulposus (NP) cells participate in the pathologic changes of intervertebral disc degeneration (IDD). ANGPTL2 is well known for its angiogenesis and proinflammatory properties and transforming growth factor β1 (TGF-β1) is also responsible for tissue fibrosis. However, the role of ANGPTL2 in IDD and whether it is related to TGF-β1 remains unclear. This study aims to explore the relation of TGF-β1 and ANGPTL2 in the degenerative process of NP cells. PATIENTS AND METHODS:We isolated NP cells of NP tissues provided from the spine fracture patients. IL-1β was used to induce the NP cells degeneration. To determine the effect of TGF-β1 and ANGPTL2 on NP cell degeneration, we regulated the cellular TGF-β1 and ANGPTL2 expression by Recombinant human protein stimulation and siRNA transfection. Quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot was employed to investigate the expression of TGF-β1, ANGPTL2, IL-6, TNF-α, collagen I, and collagen III. RESULTS:TGF-β1 overexpression aggravated the ANGPTL2, IL-6, TNF-α, collagen I, and collagen III expressions of NP cells that caused by IL-1β, which was rejected by ANGPTL2 gene silencing. Besides, the silencing of TGF-β1 weakened the ANGPTL2 expression. ANGPTL2 overexpression promoted the NP cells inflammation and fibrosis via increasing IL-6, TNF-α, collagen I, and collagen III expression, which was sharpened by a consequent increase of TGF-β1 expression. CONCLUSIONS:This study, for the first time, points that TGF-β1 aggravates degenerative NP cells inflammation and fibrosis via the mediation of ANGPTL2.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Cui L,Wei H,Li ZM,Dong XB,Wang PYdoi
10.26355/eurrev_202012_23991subject
Has Abstractpub_date
2020-12-01 00:00:00pages
12025-12033issue
23eissn
1128-3602issn
2284-0729journal_volume
24pub_type
杂志文章abstract：OBJECTIVE:The etiology of Major depressive disorder (MDD) is multifactorial but the genetic risk is an important factor. Previous studies have shown a significant interaction between serotonin and brain-derived neurotrophic factor (BDNF) in brain function. The serotonin transporter protein promoter polymorphism (5-HTTL...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVE:The study aims to construct a multi-gene risk scoring model that can be used to predict the prognosis of patients with lung squamous cell carcinoma (LUSC). PATIENTS AND METHODS:RNA-seq data from 494 LUSC tumor samples and 49 peripheral lung tissue samples were obtained from TCGA_LUSC database. Differential a...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202005_21330
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVE:Scurvy is defined as a deficiency of ascorbic acid, which is an essential exogenous vitamin in humans. Vitamin C is involved in collagen synthesis and its deficit can cause disorders of connective tissue. The most frequent symptoms are weakness, arthralgias, anorexia and depression, commonly associated with f...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201807_15433
更新日期：2018-07-01 00:00:00
abstract：OBJECTIVE:Widespread hypovitaminosis D and an increased incidence of metabolic syndrome (MetS) represent significant problems of contemporary medicine but link between them remain unresolved. We aimed to define relationship between vitamin D serum concentration and exponents of MetS. PATIENTS AND METHODS:The studies w...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:To investigated the influence of hyperthermal perfusion chemotherapy combined with immunologic therapy on the immunologic function and levels of circulating tumor cells of the advanced colorectal cancer patients with liver metastasis. PATIENTS AND METHODS:We enrolled 98 advanced colorectal cancer patients wi...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:This study aims to survey medical staff's acceptance of online Mindfulness-Based Stress Reduction (MBSR) during the Novel Coronavirus Pneumonia (NCP), and to know some information of physical and emotional response of those medical staff who worked at the forefront of COVID-19, through the playback amount of ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202010_23451
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVE:Members of the tripartite motif (TRIM) protein family contain a highly conserved N-terminal really interesting new gene (RING) domain that is involved in regulating transcriptional factors and tumor suppressors. In this study, the effects of TRIM59 expression on tumor growth were investigated in prostate canc...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-12-01 00:00:00
abstract：:Spider bites are not very common, especially in the Mediterranean area, and those affecting the ocular-palpebral region involving reconstructive surgery are particularly rare. In May 2010, the case of a Caucasian 24-year-old female patient was brought to the attention of the Dermatology Department, University of Cagli...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVE:The ubiquitin-conjugating enzyme E2C (UBE2C) has been known as a crucial factor upregulated in various tumors. The functions of UBE2C is mainly involved in the pathway protein ubiquitination. This study investigates the expression of UBE2C in gastric cancers and its correlation with overall survival rate. MA...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201803_14578
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:This study aimed to investigate the expression of TRPM7 in the serum of patients with sepsis and its influences on inflammatory factors and prognosis. PATIENTS AND METHODS:A prospective analysis was performed. 78 patients with sepsis were enrolled in the experimental group and treated from May 2015 to April ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201905_17821
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:GDF11 (Growth Differentiation factor 11) has been reported to rejuvenate skeletal muscle, heart and brain in aged mice, and the aged skeletal muscle is closely related to insulin resistance. We wondered whether GDF11 has an effect on skeletal muscle insulin resistance. MATERIALS AND METHODS:High fat diet ind...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：OBJECTIVE:Down-regulation of miR-138 is observed in a variety of cancers, which suggests that miR-138 may be involved in cancer pathogenesis. Our current work aimed to evaluate the effects of miR-138 in adriamycin (ADM)-resistant human NSCLC cells. MATERIALS AND METHODS:Cell proliferation was determined by MTT assay. ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect and mechanism of bone marrow stem cells (BMSCs) modified with CXCR-4 gene combined with stromal derived factor-1α (SDF-1α) in the treatment of acute spinal cord injury (SCI) in rats. MATERIALS AND METHODS:CXCR-4 gene was transfected by a virus. Spinal cord injury rats were randomly div...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:Age-related macular degeneration (AMD) is mainly characterized by dysfunction of retinal pigment epithelium (RPE) cells. This study aimed to investigate the protective effects of resveratrol on oxidative damaged RPE cells. MATERIALS AND METHODS:Human D407 cells were divided into normal control (NC), H2O2 tre...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201901_16786
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to explore the expression of microRNA-106a in breast cancer (BC) and to further investigate its role in BC development and the potential regulatory mechanism. PATIENTS AND METHODS:72 pairs of BC tissues and para-cancerous tissues were collected, and microRNA-106a expression was dete...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201902_17116
更新日期：2019-02-01 00:00:00
abstract：OBJECTIVE:Adipose tissue is considered an active secretory organ of adipocytokines. The principal aim of our study was to examine the changes in adipocytokines levels after weigth reduction in obese patients. MATERIALS AND METHODS:A population of 126 obese (body mass index > 30) non-diabetic outpatients was analyzed i...
journal_title：European review for medical and pharmacological sciences
pub_type： 临床试验,杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVE:Long non-coding RNAs (lncRNAs) have been identified to participate in the development and progression of various types of cancers, including non-small cell lung cancer (NSCLC). However, the expression and function of linc00261 in NSCLC has not been studied yet. We aim to explore the role and potential of linc...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201905_17810
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:Urogenital atrophy is a degenerative process that may occur during menopause causing debilitating disorders and painful symptomatology. Estrogen therapy slows the onset of atrophy, but it requires ongoing therapy to maintain its effectiveness. To mitigate the degenerative evolutions associated with menopause,...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201801_14211
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVE:We aimed to evaluate quality of sleep and self-perception risk of medication errors, in a significantly-sized sample of nurses in Italy, using a web survey. SUBJECTS AND METHODS:An anonymous questionnaire about self-perception of quality of sleep (Pittsburgh Sleep Quality Index - PSQI) and risk of medication...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202006_21699
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate clinical application of remifentanil in local anesthesia for resection of tumors in functional brain area. PATIENTS AND METHODS:Twenty-four patients were randomly divided into two groups: control group and remifentanil group. In remifentanil group remifentanil was inf...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of Lactobacillus rhamnosus GR-1 on atherosclerotic progression in apolipoprotein-E knockout (ApoE-/-) mice fed with a high-fat diet and the underlying mechanisms of its action. MATERIALS AND METHODS:Eight-week-old ApoE-/- mice were treated with Lactobacillus rhamnosus GR-1 daily for...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17722
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the relationship between micro ribonucleic acid (miR)-152-3p and chronic myeloid leukemia (CML), and to explore the underlying mechanism. PATIENTS AND METHODS:The expression level of miR-152-3p in the bone marrow of 40 CML patients and 30 normal controls was detected ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201812_16646
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVE:To explore the role of microRNA-326 in myocardial fibrosis after myocardial infarction (MI) and its underlying mechanism. MATERIALS AND METHODS:MI rat model was constructed via left anterior descending artery (LAD) ligation. Infarct tissues, infarct border zone tissues and remote zone tissues were harvested ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201808_15735
更新日期：2018-08-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in juvenile rats with nephrotic syndrome, and to explore its effects on inflammatory changes and renal injury. MATERIALS AND METHODS:24 Sprague-Dawley (SD) rats were randomly divi...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201905_17831
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:Metabolic syndrome is characterized by abdominal obesity, hypertriglyceridemia and hyperglycemia. Fatty acid binding protein 4 (FABP4), as a member of intracellular lipid chaperones, is not only engaged in lipid transport but involved in inflammation and insulin resistance. The present study was to investigat...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202012_24182
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVE:Long stress-induced noncoding transcripts 5 (LSINCT5) has been reported to be upregulated in several human cancers and related to poor prognosis. However, its involvement in esophageal squamous cell carcinoma (ESCC) remains largely unknown. We aim to evaluate the expression and putative role of LSINCT5 on the...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201906_18184
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to explore the mechanism of micro-ribonucleic acid (miR)-214-3p in regulating fracture healing in rats with osteoporosis. MATERIALS AND METHODS:A total of 30 female Sprague-Dawley rats were selected and randomly divided into 3 groups, including group A [phosphate-buffered saline...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201901_16854
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVE:Polycystic ovarian syndrome and obesity contribute to the metabolic complications for women of reproductive age. The aim of present study was to analyze the effect of abdominal obesity expressed using waist/hip ratio (WHR) in patients with polycystic ovary syndrome on metabolic parameters. PATIENTS AND METHO...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-11-01 00:00:00
abstract：BACKGROUND:Intussusception is defined as the telescoping of a segment of the gastrointestinal tract into an adjacent one. A demonstrable etiology is found in 70% to 90% of cases in adult patients, and about 40% of them are caused by a primary or secondary malignant tumor. The aims of this study were to give an overview...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:Glioma is a common aggressive cancer and a major public health problem worldwide, with high incidence, recurrence, and mortality. Circular RNA (circRNA) has been reported to be involved in glioma, but the role of circ_0079593 in glioma is still unclear. MATERIALS AND METHODS:The real-time quantitative polyme...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202001_20186
更新日期：2020-01-01 00:00:00
