Whole blood platelet aggregometry and platelet function testing.


:Platelet aggregometry has been the reference method employed to detect, diagnose, and monitor qualitative platelet disorders since the early 1960s. Lumiaggregometry and impedance-based whole blood lumiaggregometry have advantages over light transmittance aggregometry in that they provide for enhanced specimen management and increase the test sensitivity to impairment of platelet granule secretion. Whole blood lumiaggregometry detects and identifies congenital and acquired platelet plasma membrane receptor defects, metabolic pathway secretion disorders, and storage pool deficiency. Whole blood lumiaggregometry is also being applied to antiplatelet therapy monitoring and identifies aspirin and thienopyridine resistance. There is growing interest in using impedance-based whole blood lumiaggregometry for near-patient whole blood platelet analysis and antiplatelet therapy monitoring. This article will also discuss other whole blood testing processes for assessing platelet function, particularly as applied to assessing the effect of antiplatelet medication.


Semin Thromb Hemost


McGlasson DL,Fritsma GA




Has Abstract


2009-03-01 00:00:00












  • Inhibitor development in previously untreated patients with hemophilia A: a prospective long-term follow-up comparing plasma-derived and recombinant products.

    abstract::In order to assess inhibitor development in previously untreated patients (PUPs) with severe (factor VIII [FVIII]<1%) and moderate (FVIII 1 to 5%) hemophilia A, a prospective study was initiated in 1976. During the 23-year study period, 72 hemophiliacs were frequently exposed prophylactically or on demand to plasma-de...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Kreuz W,Ettingshausen CE,Zyschka A,Oldenburg J,Saguer IM,Ehrenforth S,Klingebiel T

    更新日期:2002-06-01 00:00:00

  • The Effect of BMI and Gender on Bleeding Events when Rivaroxaban Is Administered for Thromboprophylaxis Following Total Hip and Total Knee Arthroplasty.

    abstract::Rivaroxaban is approved in Europe and the United States for thromboprophylaxis following total joint arthroplasty. As the rate of obesity increases, confirming safety and efficacy in this patient population is paramount. This retrospective chart review assessed the efficacy and safety of rivaroxaban between two body m...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Krauss ES,Cronin M,Dengler N,Simonson BG,Altner K,Daly M,Segal A

    更新日期:2019-03-01 00:00:00

  • Monitoring Antiplatelet Therapy.

    abstract::The increasing use of antiplatelet therapy, particularly aspirin and oral P2Y12 inhibitors, in the prevention and management of arterial thrombosis, has stimulated extensive pharmacodynamic studies and research into tailored antiplatelet regimens. Many different methodologies have been studied for monitoring antiplate...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Orme R,Judge HM,Storey RF

    更新日期:2017-04-01 00:00:00

  • Protein C and the endothelium.

    abstract::With the continued accumulation of clinical and animal studies, it is becoming abundantly clear that the protein C anticoagulant pathway plays a critical role in the regulation of coagulation. Investigations also indicate that this pathway is intimately involved in the interaction of the coagulation and inflammatory s...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Esmon NL,Esmon CT

    更新日期:1988-04-01 00:00:00

  • Diagnostic efficacy of a simple radioimmunoassay test for fibrinogen/fibrin fragments containing the B beta 15-42 sequence.

    abstract::A simple RIA method for B beta 15-42 RPs has been evaluated in our laboratory to investigate experimental and clinical fibrinolytic states. The assay utilizes bentonite precipitation to remove cross-reacting fibrinogen. Due to the heterogeneity in molecular weights of the B beta RPs, the results are expressed as nanog...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Walenga JM,Fareed J,Mariani G,Messmore HL Jr,Bick RL,Emanuele RM

    更新日期:1984-10-01 00:00:00

  • Hemostatic Efficacy of Pathogen-Inactivated Blood Components.

    abstract::Pathogen inactivation (PI), or pathogen reduction technology, reduces the infectious risk of plasma and platelet transfusions, and also affects clotting factor activities and platelet viabilities. Plasma is treated with solvent-detergent to disrupt enveloped viruses, or with photoactive agents methylene blue plus ligh...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Ramsey G

    更新日期:2016-03-01 00:00:00

  • Protective Effects of Statins Administration in European and North American Patients Infected with COVID-19: A Meta-analysis.

    abstract::Severe acute respiratory syndrome coronavirus 2 has spread rapidly throughout the world, becoming an overwhelming global health emergency. The array of injuries caused by this virus is broad and not limited to the respiratory system, but encompassing also extensive endothelial and systemic tissue damage. Since statins...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Onorato D,Pucci M,Carpene G,Henry BM,Sanchis-Gomar F,Lippi G

    更新日期:2021-01-22 00:00:00

  • Perturbation of protease inhibitors and substrates in inflammatory arthritis.

    abstract::Defective systemic fibrinolysis and articular persistence of fibrinlike material are well recognized in RA. Perturbation of the major plasma protease inhibitors, A1AT, A2MG, and AT III, was explored in RA, psoriatic arthritis, and Reiter's syndrome. Experimental evidence is presented and assessed with respect to the p...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Rothschild BM,Thompson LD,Pifer DD,Chesney CM

    更新日期:1985-10-01 00:00:00

  • Molecular mechanisms of megakaryocyte differentiation.

    abstract::Each bone marrow megakaryocyte (MK) releases thousands of platelets into the circulation, and the underlying molecular and cellular mechanisms recently have received intense scrutiny. Genetic studies are beginning to clarify the mechanisms by which transcription factors help distinguish MK progenitors from other blood...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Schulze H,Shivdasani RA

    更新日期:2004-08-01 00:00:00

  • Onco-retroviral and lentiviral vector-based gene therapy for hemophilia: preclinical studies.

    abstract::Hemophilia A and B gene therapy requires long-term and stable expression of coagulation factor VIII (FVIII) or factor IX (FIX), respectively, and would need to compare favorably with protein replacement therapy. Onco-retroviral and lentiviral vectors are attractive vectors for gene therapy of hemophilia. These vectors...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Van Damme A,Chuah MK,Collen D,VandenDriessche T

    更新日期:2004-04-01 00:00:00

  • Thrombocytosis in childhood.

    abstract::Until recently, thrombocytoses in childhood were considered to be rare. The literature on this subject is scarce and contradictory. When thrombocytosis is defined as a platelet count of more than 500 x 10(9)/L (500 000/mm3 or 500 000/microL), the occurrence in routine examinations at pediatric hospitals can be estimat...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Sutor AH

    更新日期:1995-01-01 00:00:00

  • Stimulation of cell surface plasminogen activation by heparin and related polyionic substances.

    abstract::The functional operation of the cell surface pro-u-PA and plasminogen activating system has previously been shown to depend on the assembly of u-PA receptors, plasminogen binding sites, and their respective ligands at the focal adhesions of cell extensions. We now show that additional factors operate that affect the p...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Stephens RW,Pöllänen J,Tapiovaara H,Woodrow G,Vaheri A

    更新日期:1991-07-01 00:00:00

  • Risk of venous thromboembolism in patients with inflammatory bowel disease.

    abstract::There is ample evidence of an increased risk of venous thromboembolism (VTE) in inflammatory bowel disease (IBD). Recent large studies have quantified this risk showing that IBD patients run a 1.5 to 3.6 higher risk of developing VTE than healthy controls. The development of VTE in IBD seems to be multifactorial, resu...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Koutroumpakis EI,Tsiolakidou G,Koutroubakis IE

    更新日期:2013-07-01 00:00:00

  • Heparin, low molecular weight heparin, and derivatives in thrombosis, angiogenesis, and inflammation: emerging links.

    abstract::The key reason behind the success of heparin in thrombosis and beyond is its polypharmacological sites of action for the prevention and treatment of multifactorial diseases that will only benefit slightly with single pharmacological mechanism-based agents. Thromboembolic disorders are driven by hypercoagulable, hypera...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Mousa SA

    更新日期:2007-07-01 00:00:00

  • Anticoagulation with recombinant hirudin and danaparoid sodium in pediatric patients.

    abstract::Patients receiving heparin are at risk of developing heparin-induced thrombocytopenia (HIT). Whereas in HIT I only reversible mild thrombocytopenia occurs within the first days of heparin treatment, HIT II may lead to potentially life-threatening thromboembolic events. Pediatric patients suffering from HIT II have bee...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Severin T,Zieger B,Sutor AH

    更新日期:2002-10-01 00:00:00

  • Supportive care in pediatric cancer: the road to prevention of thrombosis.

    abstract::The survival rate of children with cancer has increased impressively to almost 80% over the last decades as a result of improved diagnostic procedures and multimodal treatment strategies. Therefore, it becomes more and more important to prevent mortality and morbidity of treatment-associated complications, including v...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: van Ommen CH,Chan AK

    更新日期:2014-04-01 00:00:00

  • Seminal vesicle protein IV and its derived active peptides: a possible physiological role in seminal clotting.

    abstract::Seminal vesicle protein 4 (SV-IV) is a highly flexible molecule that in aqueous solution behaves as a concentration-dependent self-associating system in which the degree of association (monomer <--> dimer <--> trimer equilibrium) seems to be related to its biological activities. This review reports the functional role...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Metafora S,Esposito C,Caputo I,Lepretti M,Cassese D,Dicitore A,Ferranti P,Stiuso P

    更新日期:2007-02-01 00:00:00

  • Latent heparanase facilitates VLA-4-mediated melanoma cell binding and emerges as a relevant target of heparin in the interference with metastatic progression.

    abstract::Heparanase is an endo-β-glucuronidase that enzymatically cleaves heparan sulfates (HS) and heparan sulfate proteoglycan (HSPG) structures. Heparanase expression levels by tumors were correlated with cell invasion, angiogenic activity, and poor prognosis. Heparanase can also possess pro-tumorigenic effects independent ...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Gerber U,Hoß SG,Shteingauz A,Jüngel E,Jakubzig B,Ilan N,Blaheta R,Schlesinger M,Vlodavsky I,Bendas G

    更新日期:2015-03-01 00:00:00

  • Structure and function of the factor VIII gene and protein.

    abstract::Factor (F) VIII is a large gene located near the terminus of the long arm of the X chromosome. It contains 26 exons that code for a signal peptide and a 2332 amino acid polypeptide with three different types of domains, namely A1-A2-B-A3-C1-C2. The A domains are homologous with each other and those of ceruloplasmin; s...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Thompson AR

    更新日期:2003-02-01 00:00:00

  • The Clinical Relevance of Noncriteria Antiphospholipid Antibodies.

    abstract::While lupus anticoagulant (LA), anticardiolipin antibodies (aCL), anti-β2 glycoprotein I (anti-β2GPI) antibodies represent the best available and the most widely used tests in the investigation for antiphospholipid syndrome (APS), evidence gathered in recent years indicates that other antiphospholipid antibodies (aPL)...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Bertolaccini ML,Sanna G

    更新日期:2018-07-01 00:00:00

  • The adverse effects of estrogen and selective estrogen receptor modulators on hemostasis and thrombosis.

    abstract::Agonists of the estrogen receptor include estrogens and selective estrogen receptor modulators (SERMs). Both types of compounds increase the risk for thrombosis in the arterial and the venous tree. The magnitude of the effect is influenced by potency, which depends on the type of compound and the dose. The particulars...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Artero A,Tarín JJ,Cano A

    更新日期:2012-11-01 00:00:00

  • Hyperhomocysteinemia and thrombosis.

    abstract::Homocysteine has been identified as an independent risk factor for atherosclerotic and thrombotic disease. Both arterial (cerebrovascular, carotid, coronary, and peripheral arterial) and veno-occlusive disease, jointly termed vascular occlusive disease (VOD) in this review, have been associated with hyperhomocysteinem...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Guba SC,Fonseca V,Fink LM

    更新日期:1999-01-01 00:00:00

  • Vatreptacog alfa from conception to clinical proof of concept.

    abstract::Vatreptacog alfa is a genetically engineered variant of recombinant factor VIIa (rFVIIa) containing three amino acid changes. Aspartic acid, valine, and glutamine residues replace valine, glutamic acid, and methionine at positions 158, 296, and 298, respectively. These substitutions result in considerable enhancement ...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Persson E,Olsen OH,Bjørn SE,Ezban M

    更新日期:2012-04-01 00:00:00

  • Acquired defects of fibrinolysis associated with thrombosis.

    abstract::Physiologic regulation of fibrinolysis plays an important role in the control of hypercoagulable states and thrombogenesis. Both the hereditary and acquired conditions leading to fibrinolytic deficit result in thrombotic complications leading to arterial and venous occlusive disorders. Several changes in physiologic s...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Fareed J,Hoppensteadt DA,Jeske WP,Ahmad S,Bick RL

    更新日期:1999-01-01 00:00:00

  • Time to think outside the box? Proposals for a new approach to future pharmacokinetic studies of von Willebrand factor concentrates in people with von Willebrand disease.

    abstract::Plasma-derived factor concentrates are important in the management of von Willebrand disease (VWD). We review the current literature regarding pharmacokinetic studies of von Willebrand factor (VWF) concentrates used to treat VWD. Using additional local experience of a crossover pharmacokinetic (PK) study comparing a c...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Favaloro EJ,Kershaw G,McLachlan AJ,Lloyd J

    更新日期:2007-11-01 00:00:00

  • External quality assessment for thrombin generation tests: an exploration.

    abstract::External Quality Control of Diagnostic Assays and Tests (ECAT) surveys on thrombin generation rests (TGTs) have identified that various tests show a more than 30-fold difference in time to peak (TTP). The survey included pooled normal plasmas, microparticle (MP)-depleted plasmas, and factor (F)XII-deficient patient pl...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Kluft C,Meijer P

    更新日期:2010-10-01 00:00:00

  • Diagnosis and Treatment of Trauma-Induced Coagulopathy by Viscoelastography.

    abstract::This article explores the application of viscoelastic tests (VETs) in trauma-induced coagulopathy and trauma resuscitation. We describe the advantages of VETs over conventional coagulation tests in the trauma setting and refer to previous disciplines in which VET use has reduced blood product utilization, guided prohe...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Hartmann J,Walsh M,Grisoli A,Thomas AV,Shariff F,McCauley R,Vande Lune S,Zackariya N,Patel S,Farrell MS,Sixta S,March R,Evans E,Tracy R,Campello E,Scărlătescu E,Agostini V,Dias J,Greve S,Thomas S

    更新日期:2020-03-01 00:00:00

  • Coagulation-associated enhancement of fibrinolytic activity via a neutralization of PAI-1 activity.

    abstract::Total fibrinolytic activity in the vasculature is finely tuned by the balance between tissue plasminogen activator and plasminogen activator inhibitor type 1 (PAI-1). Although PAI-1 targets plasminogen activators, it also reacts with other serine proteases such as thrombin and factor Xa. The latter was shown to intera...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Urano T,Ihara H,Suzuki Y,Takada Y,Takada A

    更新日期:2000-01-01 00:00:00

  • Laboratory diagnosis of antithrombin and heparin cofactor II deficiency.

    abstract::Antithrombin activity should probably be determined in persons with early onset of recurrent thrombosis, especially if a family history is present. The initial screening test should be a heparin cofactor assay optimized to reduce the contribution of heparin cofactor II. If the heparin cofactor assay is low, an antigen...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章,评审


    authors: Tollefsen DM

    更新日期:1990-04-01 00:00:00

  • Prophylaxis of venous thromboembolism in stroke patients.

    abstract::Venous thromboembolism is a common complication in patients with acute thrombotic stroke. Estimates of the frequency of deep vein thrombosis (DVT) in untreated patients range from 20 to 75%. This wide range reported depends on the methods used to detect DVT and, importantly, on the degree of lower limb paralysis. Most...

    journal_title:Seminars in thrombosis and hemostasis

    pub_type: 杂志文章


    authors: Turpie AG

    更新日期:1997-01-01 00:00:00