Abstract：
:We sought to develop a protocol to isolate and culture porcine Wirsung duct cells in order to determine their potency to differentiate into insulin-expressing beta-like cells. The porcine Wirsung duct isolated by a surgical microdissection was digested with collagenase P and trypsin to dissociate ductal cells. These elements were cultured in serum-free supplemented media: for 2 weeks. Thereafter the cells were exposed to varying concentrations of glucose (0, 5.6, 17.8, and 25 mmol/L) to induce a beta-like phenotype, as identified by immunohistochemical staining. Cell growth proceeded slowly for the first 2 weeks of culture. After glucose induction for 2 weeks, they formed pancreatic islet-like structures. These cells were stained for the pancreatic ductal cell marker cytokeratin-19 (CK-19) and the pancreatic endocrine markers insulin and glucagon. After the second week, 90% of cells were positive for CK-19. Up to 20.1% of the cells in pancreatic 3-dimensional structures induced by 17.8 mmol/L glucose were positive for insulin, and <3.2%, for glucagon. The positive ratio of immunoreactive staining was dependent on the glucose concentration; 17.8 mmol/L glucose effectively stimulated insulin- and glucagon-secreting cells. We concluded that porcine Wirsung duct cells were capable of proliferation with the potential to differentiate toward beta cells upon glucose induction in vitro.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Gioviale MC,Damiano G,Montalto G,Buscemi G,Romano M,Lo Monte AIdoi
10.1016/j.transproceed.2009.02.062subject
Has Abstractpub_date
2009-05-01 00:00:00pages
1363-6issue
4eissn
0041-1345issn
1873-2623pii
S0041-1345(09)00206-1journal_volume
41pub_type
杂志文章abstract：BACKGROUND:Recently, preservation using oxygenated perfluorocarbon (the two-layer method) has shown beneficial effects on islet yield and viability. In this paper, we apply this concept on isolation processes to examine the effectiveness of oxygenation. METHODS:Rat pancreata were digested using four different methods:...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.12.232
更新日期：2005-01-01 00:00:00
abstract：:These results suggest (1) that ALS + BM fractions can be used to promote tolerance in long-term graft survivors; (2) that the presence of apparent BM cells of donor origin in the allograft in normally functioning kidneys may be related to the mechanism of allograft prolongation, and (3) that some promise of possible s...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：BACKGROUND:Complete immune tolerance is the chief goal in organ transplantation. This study aimed to evaluate patients who successfully withdrew from immunosuppressive (IS) agents after living donor liver transplantation (LDLT). MATERIALS AND METHODS:A retrospective review of all adult LDLT from July 1999 to March 201...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2015.12.093
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate the outcomes of liver transplant recipients who became pregnant after transplantation. METHODS:The clinical data of all patients who underwent liver transplantation between January 2007 and December 2016 in our liver transplantation institute were reviewed. The following...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.04.023
更新日期：2017-10-01 00:00:00
abstract：BACKGROUND:Despite significant advances in kidney transplantation, long-term graft survival has not dramatically improved leading to strategies to change immunosuppression during the posttransplantation period. Proliferation signal inhibitors (PSI) sirolimus or everolimus possess immunosuppressive and antiproliferative...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.01.097
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:Mycobacterium abscessus is a ubiquitous, rapidly growing mycobacterium that colonizes organic surfaces. It is a potential pathogen, especially in immunosuppressed patients, including transplant recipients in whom disease can range from localized cutaneous lesions to disseminated infections. The purpose of th...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.08.004
更新日期：2010-10-01 00:00:00
abstract：:Mitomycin-C (MMC) is categorized as an agent that causes genotoxic stress by triggering various intracellular signaling pathways. We have previously shown that MMC pretreatment of highly immunogenic crude islets leads to significant prolongation of graft survival in a rat-to-mouse model. In the present study, we exami...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.04.029
更新日期：2004-05-01 00:00:00
abstract：BACKGROUND:The dog has served as an important experimental model for biomedical research such as transplantation and developing immunosuppressive agents. Although major histocompatibility complex (MHC) in dogs is a dominant factor of graft rejection, it has not been well investigated in dogs compared with human. For th...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.11.045
更新日期：2015-04-01 00:00:00
abstract：UNLABELLED:A primary reason to eliminate corticosteroids from immunosuppressive regimens in solid organ transplant recipients is improved cardiovascular risk profiles. Although a number of studies have documented that corticosteroid withdrawal (CSWD) regimens reduce hypertension, hyperlipidemia, diabetes, and weight ga...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.01.060
更新日期：2005-03-01 00:00:00
abstract：:Cytomegalovirus (CMV) end-organ disease is a serious, frequent complication after allogenic stem cell transplantation (Allo-SCT). There are two prevention strategies: universal prophylaxis and preemptive therapy. Preemptive therapy is administered based on the results of sensitive techniques that detect the viral infe...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.05.050
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND:Hepatitis C has been associated with an increased incidence of diabetes mellitus (DM) following renal transplantation (RT). METHODS:Patients who underwent RT between 1985 and 2001 were excluded if they showed DM prior to RT, graft survival of less than 90 days, and unknown anti-HCV status (n=15). Two groups...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/s0041-1345(03)00611-0
更新日期：2003-08-01 00:00:00
abstract：:Lung transplantation for end-stage lung disease results in prolonged survival and improved pulmonary function. However, the shortage of donor lungs has been a major limiting factor in Korea. We sought to investigate the number and utilization of donor lungs by the five institutions performing LTx in Korea, retrospecti...
journal_title：Transplantation proceedings
pub_type： 杂志文章,多中心研究
doi：10.1016/j.transproceed.2011.12.060
更新日期：2012-05-01 00:00:00
abstract：:Recipients of allotransplants are more susceptible to viral infections, among which the human papilloma virus infection is an independent factor inducing precancerous lesions and cancers of both the anogenital and the cervicocephalic region. MATERIALS AND METHODS:The study included a group of 69 allogenic kidney tran...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.05.008
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Poor health literacy is associated with inferior outcomes in kidney transplant recipients, and knowledge remains suboptimal in this population. The goal of this study was to characterize the health literacy, kidney transplant knowledge, medication beliefs, and education satisfaction in a cohort of patients w...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2016.07.018
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:The purpose of this study was to describe the initial experience with alemtuzumab as induction followed by steroid-free immunosuppression in kidney transplantation. METHODS:One hundred patients who received renal transplants from living and deceased donors were followed for a median period of 12 months (ran...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.02.026
更新日期：2008-04-01 00:00:00
abstract：:The success of human islet isolation is hampered by the varied and unpredictable outcomes of the islet isolation procedure. Pancreata which meet well-defined criteria are no guarantee for success. Interindividual variations may contribute to the differences in isolation outcomes. The present study examined several str...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.12.022
更新日期：2008-03-01 00:00:00
abstract：PURPOSE:Oxidative stress is one of the most important mechanisms to explain genesis of the complications in the chronic progression of diabetes. In this investigation we studied the effects of pancreas transplantation (PT) on the imbalance caused by excessive production of free oxygen radicals by antioxidant defenses o...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.01.050
更新日期：2008-03-01 00:00:00
abstract：:This report describes the clinical characteristics and demographics of patients enrolled into this rescue trial for patients experiencing refractory rejection after liver transplantation. Actuarial graft and patient survival at 12 months postconversion was 50% and 72%, respectively. Actual treatment success at 3 month...
journal_title：Transplantation proceedings
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：1993-02-01 00:00:00
abstract：INTRODUCTION:Ureter stenosis in renal transplantation patients is a relatively frequent complication that negatively conditions graft evolution. The use of ureteral stents is a valid treatment alternative to the use of double-J catheters in patients for whom surgery is not contemplated or after surgical recurrence. We ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.09.048
更新日期：2018-03-01 00:00:00
abstract：:Interleukin-6 (IL-6) has diagnostic importance as one of the earliest indicators of inflammation, tissue damage, hypoxia, or infection. In contrast to the time-consuming immunoassays actually used in many laboratories, results of a new IL-6 bedside test, which is interpreted visually or photometrically, are available ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.12.015
更新日期：2007-03-01 00:00:00
abstract：:As liver transplantation (LT) is now being performed with excellent 1-year graft survival rates of 85% to 90%, attention has been shifted to reducing long-term complications of calcineurin inhibitors (CNI). We randomized LT patients (2:1) who displayed renal dysfunction under CNI treatment to either mycophenolate mofe...
journal_title：Transplantation proceedings
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.transproceed.2004.10.008
更新日期：2004-11-01 00:00:00
abstract：BACKGROUND:Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.05.110
更新日期：2010-07-01 00:00:00
abstract：:Liver transplantation, the only effective treatment for end-stage disease, is limited by donor availability. Cell transplantation offers the possibility to restore liver mass and function. Cryopreserved primary hepatocytes that can be thawed as needed might address this problem. Hepatocytes were harvested from a male ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2012.03.050
更新日期：2012-05-01 00:00:00
abstract：INTRODUCTION:Previous studies of the safety of renal autotransplantation (RAT) have been limited by a lack of evidence. Because of the influence of the opposite kidney, it is difficult to evaluate actual renal function. This study evaluated the actual results of RAT by collecting only cases involving a solitary kidney....
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.09.030
更新日期：2017-11-01 00:00:00
abstract：:There was no statistical significance to the differences in waiting time for cadaveric renal transplant by race. Whether for first transplant or second or greater, any differences in waiting time could not be accounted for by the recipient's race. CAUC made up 58% of the waiting list, 65% of the recipients, and 87% of...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：INTRODUCTION:Cardiovascular disease is the primary cause of death among kidney transplant recipients (KTRs), whereas chronic allograft nephropathy (CAN) is the main reason leading to end-stage chronic kidney disease. The etiologies of both entities include immunologic and nonimmunologic factors. The management of modif...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2012.09.010
更新日期：2012-11-01 00:00:00
abstract：:BK virus-associated nephropathy (BKVN) can cause clinically significant viral infection in renal transplant recipients, leading to allograft dysfunction and loss. The usual management of BKVN involves the reduction of immunosuppression and the addition of leflunomide, quinolones, and cidofovir, but the rate of graft l...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2015.01.012
更新日期：2015-03-01 00:00:00
abstract：:Reuse of liver graft for transplantation is extremely uncommon. We report the 1st case of reuse of liver graft from a recipient who had hepatitis B virus (HBV) infection, 11 years after the 1st transplantation. Our relay liver transplantation challenged conventional thinking because of late reuse of graft in the prese...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2015.09.036
更新日期：2015-11-01 00:00:00
abstract：BACKGROUND:Graft rejection after intestinal transplantation remains challenging. We aimed to use endoscopy for rejection prediction. MATERIALS AND METHODS:Patients ≥7 years old who underwent intestinal transplantation between November 2016 and September 2019 were prospectively enrolled. Magnifying endoscopy under narr...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2020.10.002
更新日期：2021-01-01 00:00:00
abstract：:To determine the patterns of infectious complications in renal transplant recipients in our center, we evaluated 48 patients (29 men and 19 women) who were transplanted between 1994 and 2003. The average age of the patients was 29 years. Thirty (62.5%) and 18 (37.5%) transplants were from living related and cadaveric ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.05.009
更新日期：2005-07-01 00:00:00
