Ethanol and cyclophosphamide induce similar nephrotoxic effects: possible role for Nox4 and superoxide.

Abstract:

:We tested the hypothesis that ethanol consumption would aggravate the renal damage induced by cyclophosphamide (CYP). Male C57BL/6J mice from control (n=8) and CYP (n=12) groups had free access to filtered water and standard rodent chow for 12 weeks. Then, 24 h before euthanasia mice received an intraperitoneal injection of saline or CYP (300 mg/kg). Mice from ethanol (n=8) and CYP + ethanol (n=12) groups had free access to increasing doses of ethanol for 12 weeks. Twenty four hours before euthanasia, mice from ethanol and CYP + ethanol groups received an intraperitoneal injection of saline or CYP, respectively. Ethanol, CYP or the association of both drugs augmented serum levels of creatinine and increased the levels of superoxide (O2•-) generation and thiobarbituric acid reactive substances (TBARS) in the renal cortex. Up-regulation of Nox4 and increased activity of superoxide dismutase (SOD) were detected in the renal cortex of mice treated with ethanol, CYP or the combination of these drugs. However, these molecular alterations induced by CYP were not potentiated by ethanol consumption. Our findings revealed that chronic ethanol consumption had no potentiating effect on the nephrotoxic effects displayed by CYP. It is possible that the combination of these drugs showed no synergistic effect because they share the same molecular mechanisms of renal toxicity.

authors

Sousa AH,Do Vale GT,Silva CB,Awata WM,Pinheiro LC,Tirapelli CR

doi

10.1139/cjpp-2020-0246

subject

Has Abstract

pub_date

2020-11-11 00:00:00

eissn

0008-4212

issn

1205-7541

pub_type

杂志文章
  • Endothelium derived relaxing factor release from canine coronary artery by leukocytes.

    abstract::Lectins, known to recognize endothelial cell adhesion molecules, have been shown to release endothelium-derived relaxing factor (EDRF) from blood vessels. We investigated the effects of different leukocyte-type cells to determine if these cells, by interacting with the endothelium, could release EDRF from the circumfl...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y95-052

    authors: Kleha JF,Devesly P,Johns A

    更新日期:1995-03-01 00:00:00

  • Endocannabinoid and N-acylethanolamide levels in rat brain and spinal cord following systemic dipyrone and paracetamol administration.

    abstract::The cannabinoid system has been suspected to play a role in the mechanisms of action of dipyrone and paracetamol. Our purpose was to measure the local endocannabinoid and N-acylethanolamide levels in the brain and spinal cord of rats following dipyrone and paracetamol administration. Nociception was assessed 1, 5, and...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/cjpp-2019-0015

    authors: Topuz RD,Gunduz O,Karadag CH,Dokmeci D,Ulugol A

    更新日期:2019-11-01 00:00:00

  • Rat atrial muscle responses with caffeine: dose-response, force frequency, and postrest contractions.

    abstract::Caffeine has been reported to have a positive and (or) a negative inotropic effect on cardiac muscle. In this study, the force-frequency and postrest characteristics of rat atrium were studied in the presence of caffeine (1.0-10 mM) to see if the interval between beats affected the response of cardiac muscle to caffei...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y92-034

    authors: MacIntosh BR,Posner P,Lobo J,Harms R

    更新日期:1992-02-01 00:00:00

  • Studies of the innervation of rabbit myometrium and cervix.

    abstract::We characterized the innervation of isolated circular and longitudinal-oriented muscle strips from the nulliparous rabbit uterus and cervix by field stimulation (FS). FS with increasing frequency (2.5-50 pps) and voltage (2.5-70 V) caused graded increases in isometric contraction with no relaxation or inhibition of sp...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y89-131

    authors: Bulat R,Kannan MS,Garfield RE

    更新日期:1989-08-01 00:00:00

  • Caffeine and theanine exert opposite effects on attention under emotional arousal.

    abstract::Tea is perceived as more relaxing than coffee, even though both contain caffeine. L-theanine in tea may account for the difference. Consumed together, caffeine and theanine exert similar cognitive effects to that of caffeine alone, but exert opposite effects on arousal, in that caffeine accentuates and theanine mitiga...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/cjpp-2016-0498

    authors: Giles GE,Mahoney CR,Brunyé TT,Taylor HA,Kanarek RB

    更新日期:2017-01-01 00:00:00

  • Doxepin, a tricyclic antidepressant, binds to normal, intact astroglial cells in cultures and inhibits the isoproterenol-induced increase in cyclic AMP production.

    abstract::Doxepin binds to intact astrocytes in primary cultures. The binding is competitively displaceable by excess cold doxepin and it is pronounced (Bmax = 27 nmol/mg protein) but the affinity is rather low (KD = 30 microM). The binding is inhibited by other antidepressants (amitriptyline, desipramine, tranylcypromine, ipri...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y80-230

    authors: Hertz L,Richardson JS,Mukerji S

    更新日期:1980-12-01 00:00:00

  • The 1990 Borden Award Lecture. Dietary regulation of fatty acid and triglyceride metabolism.

    abstract::The level of circulating triacylglycerols is determined by the balance between their delivery into the plasma and their removal from it. Plasma triacylglycerols are derived either from dietary fat as chylomicrons or from endogenous hepatic synthesis as very low density lipoproteins. Their removal occurs through the ac...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y91-242

    authors: Herzberg GR

    更新日期:1991-11-01 00:00:00

  • Calcium in excitation--contraction coupling of frog skeletal muscle.

    abstract::A principal step in the process leading to muscle contraction is the intracellular release of Ca2+. We have detected and compared some physical and chemical events that reflect Ca2+ release in contracting frog skeletal muscle cells, described the effects of some agents that are believed to alter intracellular Ca2+ rel...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y82-068

    authors: Taylor SR,Lopez JR,Griffiths PJ,Trube G,Cecchi G

    更新日期:1982-04-01 00:00:00

  • Impact of enalapril therapy on in vitro coronary artery responsiveness in pacing-induced heart failure.

    abstract::In vitro coronary artery responsiveness to angiotensin I, angiotensin II, noradrenaline, phenylephrine, BHT 920, and potassium chloride together with functional relaxation to acetylcholine was investigated in dogs with pacing-induced heart failure treated with enalapril (oral administration of 10 mg.day-1) for a mean ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y92-199

    authors: Forster C,Larosa G,Armstrong PW

    更新日期:1992-10-01 00:00:00

  • PACAP enhances stimulation-induced norepinephrine release in canine pancreas in vivo.

    abstract::The present study was to investigate whether pituitary adenylate cyclase activating polypeptide (PACAP) can modify norepinephrine (NE) release in response to pancreatic nerve stimulation in anesthetized dogs. Plasma catecholamine concentrations in aortic and superior pancreaticoduodenal (SPD) venous blood were determi...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/cjpp-76-7-8-788

    authors: Yamaguchi N,Fukushima Y

    更新日期:1998-07-01 00:00:00

  • Changes in peripheral pulmonary vascular resistance after ioxaglate infusion in anesthetized dogs.

    abstract::The pulmonary vascular resistance in a peripheral portion of the pulmonary vascular bed can be determined with a catheter wedged in a peripheral branch of the pulmonary artery, from the pressure increase induced by infusion of mixed venous blood at known flow rates. The volume of the wedged vascular bed can be estimat...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:

    authors: Chabot F,Saunier C,Schrijen F

    更新日期:1997-01-01 00:00:00

  • Forskolin and phosphodiesterase inhibitors release adenosine but inhibit morphine-evoked release of adenosine from spinal cord synaptosomes.

    abstract::The effects of forskolin, Ro 20-1724, rolipram, and 3-isobutyl-1-methylxanthine (IBMX) on morphine-evoked release of adenosine from dorsal spinal cord synaptosomes were evaluated to examine the potential involvement of cyclic AMP in this action of morphine. Ro 20-1724 (1-100 microM), rolipram (1-100 microM), and forsk...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y91-133

    authors: Nicholson D,White TD,Sawynok J

    更新日期:1991-06-01 00:00:00

  • Effect of folic acid deficiency on pregnant rats and their offspring.

    abstract::Two groups of 63-day-old female Wistar rats were fed a folic acid deficient diet, based on 20% of vitamin-free casein and containing 1% of succinylsulfathiazole, for 5 weeks (group A) and 9 weeks (group B) before being bred, and the same diet was continued through pregnancy and lactation. Three out of eleven (21.3%) a...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y77-060

    authors: Tagbo IF,Hill DC

    更新日期:1977-06-01 00:00:00

  • Inhibition of small conductance K+ -channels attenuated melatonin-induced relaxation of serotonin-contracted rat gastric fundus.

    abstract::The aim of this study was to investigate the effects of melatonin on rat gastric fundus smooth muscle. Melatonin (10(-4) to 10(-3) M) had no effect on the basal tone of gastric smooth muscle. After precontraction with carbachol (10(-6) M) or serotonin (10(-7) M), melatonin caused a concentration dependent inhibitory a...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:

    authors: Storr M,Schusdziarra V,Allescher HD

    更新日期:2000-10-01 00:00:00

  • Role of cyclic AMP response element binding protein (CREB) in angiotensin II-induced responses in vascular smooth muscle cells.

    abstract::Cyclic AMP response element (CRE) binding protein (CREB) is a nuclear transcription factor that regulates the transcription of several genes containing the CRE sites on their promoters. CREB is activated by phosphorylation on a key serine residue, Ser311, in response to a wide variety of extracellular stimuli includin...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/cjpp-2020-0531

    authors: Truong V,Anand-Srivastava MB,Srivastava AK

    更新日期:2021-01-01 00:00:00

  • Effects of alterations in maternal thyroid metabolism on embryonic thyroid development in the chick.

    abstract::The cause of embryonic thyroidal enlargement in the progeny of chickens fed goitrogenic rapeseed meal (RSM) has been investigated. It has been found that eggs from birds fed RSM are low in iodine. The low concentration of iodine in the eggs of the goitrous birds is apparently due to diversion of a large proportion of ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y76-038

    authors: March BE,Leung P

    更新日期:1976-06-01 00:00:00

  • Canine coronary venous pressures: responses to positive inotropism and vasodilation.

    abstract::The epicardial coronary venous pressure in 16 dogs was compared with coronary arterial pressure as well as aortic, intraventricular, and intramyocardial pressures. Partial aortic occlusion augmented intraventricular (IVP), intramyocardial (IMP), aortic (AP), and coronary arterial pressures. Peripheral coronary venous ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y83-033

    authors: Klassen GA,Armour JA

    更新日期:1983-03-01 00:00:00

  • Hypertensive factor: calcium stimulatory activity obtained from different tissues and animal species.

    abstract::It was recently shown that a peptide (hypertensive factor, HF) isolated from erythrocyte hemolysates from spontaneously hypertensive rats induced a prolonged elevation of blood pressure in normotensive rats. In addition, the peptide produced a marked stimulation of the in vitro uptake of lanthanum-resistant calcium by...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y88-209

    authors: Wright GL,Huang BS,Johnson PJ,McCumbee WD

    更新日期:1988-10-01 00:00:00

  • Hindlimb skeletal muscle blood flow during sympathetic nerve block before and during acute anemia.

    abstract::The role of sympathetic innervation in the regulation of hindlimb skeletal muscle blood flow (QL) and metabolism was studied prior to and during acute anemia in anesthetized, paralyzed, and ventilated dogs (n = 8). Neural activity in the sciatic nerve was reversibly cold blocked for a 15-min period at control hematocr...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y88-189

    authors: Kubes P,Cain SM,Chapler CK

    更新日期:1988-09-01 00:00:00

  • Comparative aspects and temperature dependence of [3H]1,4-dihydropyridine Ca2+ channel antagonist and activator binding to neuronal and muscle membranes.

    abstract::Binding of [3H]nitrendipine, [3H]nimodipine, and (+)[3H]PN 200-110 to microsomal preparations of guinea pig smooth and cardiac muscle and brain synaptosomes revealed high affinity interaction with KD values in the sequence, (+)PN 200-110 greater than nitrendipine greater than nimodipine. Bmax values for a particular t...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y87-228

    authors: Rampe D,Luchowski E,Rutledge A,Janis RA,Triggle DJ

    更新日期:1987-07-01 00:00:00

  • Regulation of postsynaptic responses by calcitonin gene related peptide and ATP at developing neuromuscular junctions.

    abstract::Neuronal factors co-released with neurotransmitters may play an important role in synapse development and function. Calcitonin gene related peptide (CGRP) and adenosine 5'-triphosphate (ATP), two principal neuromodulators present in the motor nerve terminals, were studied for their roles and mechanisms during early de...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y95-149

    authors: Lu B,Fu WM

    更新日期:1995-07-01 00:00:00

  • The impact of doxorubicin and dexrazoxane injection of prepubertal female rats on pregnancy outcome and cardiac function postpartum.

    abstract::Childhood cancer survivors can develop significant cardiac dysfunction in adulthood as a consequence of their cancer treatment. Studies have linked heart failure during pregnancy to childhood doxorubicin (DOX) exposure. We hypothesized that DOX injection would reduce cardiac function peripartum and that DOX-treated da...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y2012-126

    authors: Calvé A,Noiles W,Sebag IA,Chalifour LE

    更新日期:2012-11-01 00:00:00

  • The role of the posterior parietal cortex in coordinate transformations for visual-motor integration.

    abstract::Lesion to the posterior parietal cortex in monkeys and humans produces spatial deficits in movement and perception. In recording experiments from area 7a, a cortical subdivision in the posterior parietal cortex in monkeys, we have found neurons whose responses are a function of both the retinal location of visual stim...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y88-078

    authors: Andersen RA,Zipser D

    更新日期:1988-04-01 00:00:00

  • Endurance exercise in a rat model of metabolic syndrome.

    abstract::We have measured the responses to endurance exercise training on body composition and glucose regulation, as well as cardiovascular and liver structure and function in rats fed a high carbohydrate and high fat (HCHF) diet as a model of human metabolic syndrome. Male Wistar rats (9-10 weeks old) were randomly allocated...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y2012-097

    authors: Cameron I,Alam MA,Wang J,Brown L

    更新日期:2012-11-01 00:00:00

  • Mechanical and electrophysiological effects of a hydroxyphenyl-substituted tetrahydroisoquinoline, SL-1, on isolated rat cardiac tissues.

    abstract::The mechanisms of the positive inotropic action of a new synthetic tetrahydroisoquinoline compound, SL-1, were investigated in isolated rat cardiac tissues and ventricular myocytes. SL-1 produced a rapidly developing, concentration-dependent positive inotropic response in both atrial and ventricular muscles and a nega...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y95-727

    authors: Chang GJ,SU MJ,Lee PH,Lee SS,Liu KC

    更新日期:1995-11-01 00:00:00

  • Differential sensitivity of hepatic microsomal aryl hydrocarbon hydroxylase to the inhibitory effect of SKF 525-A in rats of varying age, hormonal- and drug-induced status.

    abstract::The inhibition of hepatic microsomal aryl hydrocarbon hydroxylase (AHH) activity by SKF 525-A in vitro was studied in hepatic microsomes from rats of varying age, sex hormone status, and drug treatment. The concentration of SKF 525-A required to produce a 50% inhibition of AHH activity, the IC50, was determined for th...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y82-156

    authors: Gontovnick LS,Bellward GD

    更新日期:1982-08-01 00:00:00

  • Extracellular matrix and airway smooth muscle interactions: a target for modulating airway wall remodelling and hyperresponsiveness?

    abstract::The airway smooth muscle from asthmatic airways produces increased amounts and an altered composition of extracellular matrix proteins. The extracellular matrix can in turn influence the phenotype and function of airway smooth muscle cells, affecting the biochemical, geometric, and mechanical properties of the airway ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/Y07-050

    authors: Tran T,Halayko AJ

    更新日期:2007-07-01 00:00:00

  • Temporal coordination in bimanual actions.

    abstract::The issue of bimanual temporal coordination in human subjects is discussed for three selected movement paradigms: (i) simple, symmetric, bimanual finger movements, (ii) bimanual unloading, and (iii) a complex bimanual pull and grasp task. Temporal synchronization was found for all three experiments and was least varia...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y94-084

    authors: Wiesendanger M,Kaluzny P,Kazennikov O,Palmeri A,Perrig S

    更新日期:1994-05-01 00:00:00

  • Inflammatory cytokines and malnutrition as related to risk for cardiovascular disease in hemodialysis patients.

    abstract::Malnutrition and inflammation are associated with end-stage renal disease (ESRD). Interleukin (IL)-6 and tumor necrosis factor alpha (TNF-alpha) powerfully predict death from cardiovascular disease. The aim of our study was to establish an association between markers of inflammation and parameters of malnutrition in p...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/Y08-018

    authors: Perunicic-Pekovic G,Pljesa S,Rasic-Milutinovic Z,Stankovic S,Ilic M,Maletic R

    更新日期:2008-04-01 00:00:00

  • Bradykinin B2 receptor antagonists: development and applications.

    abstract::Most physiological and pathophysiological responses to bradykinin are mediated by G-protein-coupled receptors designated B2 receptors. Discovery of antagonists for these receptors has brought about a revolution in research in the kinin field. The chemistry and development of antagonists for B2 kinin receptors are disc...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y95-106

    authors: Stewart JM

    更新日期:1995-07-01 00:00:00