Abstract:
:We tested the hypothesis that ethanol consumption would aggravate the renal damage induced by cyclophosphamide (CYP). Male C57BL/6J mice from control (n=8) and CYP (n=12) groups had free access to filtered water and standard rodent chow for 12 weeks. Then, 24 h before euthanasia mice received an intraperitoneal injection of saline or CYP (300 mg/kg). Mice from ethanol (n=8) and CYP + ethanol (n=12) groups had free access to increasing doses of ethanol for 12 weeks. Twenty four hours before euthanasia, mice from ethanol and CYP + ethanol groups received an intraperitoneal injection of saline or CYP, respectively. Ethanol, CYP or the association of both drugs augmented serum levels of creatinine and increased the levels of superoxide (O2•-) generation and thiobarbituric acid reactive substances (TBARS) in the renal cortex. Up-regulation of Nox4 and increased activity of superoxide dismutase (SOD) were detected in the renal cortex of mice treated with ethanol, CYP or the combination of these drugs. However, these molecular alterations induced by CYP were not potentiated by ethanol consumption. Our findings revealed that chronic ethanol consumption had no potentiating effect on the nephrotoxic effects displayed by CYP. It is possible that the combination of these drugs showed no synergistic effect because they share the same molecular mechanisms of renal toxicity.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Sousa AH,Do Vale GT,Silva CB,Awata WM,Pinheiro LC,Tirapelli CRdoi
10.1139/cjpp-2020-0246subject
Has Abstractpub_date
2020-11-11 00:00:00eissn
0008-4212issn
1205-7541pub_type
杂志文章abstract::Lectins, known to recognize endothelial cell adhesion molecules, have been shown to release endothelium-derived relaxing factor (EDRF) from blood vessels. We investigated the effects of different leukocyte-type cells to determine if these cells, by interacting with the endothelium, could release EDRF from the circumfl...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y76-038
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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更新日期:1995-07-01 00:00:00