Abstract：
:Sheep and goats differ in diet selection, which may reflect different abilities to deal with the ingestion of plant secondary metabolites. Although saliva provides a basis for immediate oral information via sensory cues and also a mechanism for detoxification, our understanding of the role of saliva in the pre-gastric control of the intake of herbivores is rudimentary. Salivary proteins have important biological functions, but despite their significance, their expression patterns in sheep and goats have been little studied. Protein separation techniques coupled to mass spectrometry based techniques have been used to obtain an extensive comprehension of human saliva protein composition but far fewer studies have been undertaken on animals' saliva. We used two-dimensional electrophoresis gel analysis to compare sheep and goats parotid saliva proteome. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to identify proteins. From a total of 260 sheep and 205 goat saliva protein spots, 117 and 106 were identified, respectively. A high proportion of serum proteins were found in both salivary protein profiles. Major differences between the two species were detected for proteins within the range of 25-35 kDa. This study presents the parotid saliva proteome of sheep and goat and highlights the potential of proteomics for investigation relating to intake behavior research.
journal_name
Physiol Behavjournal_title
Physiology & behaviorauthors
Lamy E,da Costa G,Santos R,Capela E Silva F,Potes J,Pereira A,Coelho AV,Sales Baptista Edoi
10.1016/j.physbeh.2009.07.002subject
Has Abstractpub_date
2009-10-19 00:00:00pages
393-401issue
4eissn
0031-9384issn
1873-507Xpii
S0031-9384(09)00251-0journal_volume
98pub_type
杂志文章abstract：:Electrophysiological and behavioral experiments were performed to determine whether the taste system of the aquatic salamander, axolotl, discriminates taste stimuli. Taste responses were recorded extracellularly from the glossopharyngeal nerve bundle. The behavioral responses of axolotls towards various concentrations...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(94)90269-0
更新日期：1994-07-01 00:00:00
abstract：:Alteration of the sodium concentration in the cerebrospinal fluid (CSF) of sheep induces reciprocal changes in sodium appetite. Similar studies have now been performed in cattle. Heifers were prepared with a unilateral parotid fistula and guide tubes were implanted in the skull for the introduction of probes into the ...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(87)90374-x
更新日期：1987-01-01 00:00:00
abstract：:Male hooded rats were castrated and implanted with Silastic capsules (1.57 mm i.d.; 3.18 mm o.d.) having a testosterone-filled space 0, 7, 22, 60, or 90 mm long. All animals were returned to their original group cages for a three-week period to allow hormone concentrations and behavioral tendencies to stabilize. Each ...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(90)90336-3
更新日期：1990-09-01 00:00:00
abstract：:In experiment 1, electrical stimulation of the posterior hippocampus was shown to produce state-dependent learning (SDL) for a step-out inhibitory avoidance task in rats. Stimulation sites in either the right or left hippocampus were equally effective in producing this effect. Similarly, the presence or absence of aft...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(85)90091-5
更新日期：1985-01-01 00:00:00
abstract：:Electric shocks lead to lasting behavioral deficits in rodents, and as such are often used to model post-traumatic stress disorder (PTSD) in the laboratory. Here we show that a single exposure of rats to 3 mA-strong shocks results in a marked social avoidance that lasts at least 28 days; moreover, the response intensi...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2008.01.023
更新日期：2008-06-09 00:00:00
abstract：:Female rats can show mounting behavior towards isosexual or heterosexual conspecifics. The present experiments were designed to study whether the prenatal presence of male fetuses would affect display of this mounting behavior in adulthood. Therefore mounting behavior, after gonadectomy and during continuous treatment...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(82)90167-6
更新日期：1982-06-01 00:00:00
abstract：:Previous work in our laboratory has found that whereas medial septal lesions impaired an operant left-right delayed alternation task in rats, the lesion also facilitated the performance of rats on a cued go/no-go discrimination task with a delay between the cue and the required response. These findings suggested to us...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(95)02044-6
更新日期：1995-12-01 00:00:00
abstract：:Postcopulatory genital autogrooming was studied in rats following desensitization of the glans penis due to topical application of an anesthetic ointment or to surgical transection of the dorsal penile nerve. These treatments sharply reduced the number of mounts resulting in intromission, but genital autogrooming was ...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(89)90228-x
更新日期：1989-05-01 00:00:00
abstract：:The activity-stress ulcer procedure consists of housing rats in running-wheel activity cages while at the same time, restricting their food intake to 1 hr per day. Male Wistar rats which had been habituated with ad lib feeding to the running-wheel cage environment for 3 days prior to the restricted feeding phase, deve...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(82)90247-5
更新日期：1982-10-01 00:00:00
abstract：:Searching for food by extensive digging is one of the most important aspects of life of subterranean rodents. We studied the effect of extrinsic (substrate quality) and intrinsic factors (sex and body mass) upon the cost of burrowing, expressed as digging metabolic rate (DMR) in two African mole-rat species (Bathyergi...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2009.10.007
更新日期：2010-01-12 00:00:00
abstract：:Learned aversions developed following sublethal ingestion of poisons are reported to be long-lived in many pest species. This study aimed to identify the duration of bait aversions following poisoning with a common poison (cyanide) used for possum control in New Zealand. Groups of possums with bait aversions (followin...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/s0031-9384(97)00175-3
更新日期：1997-10-01 00:00:00
abstract：:Chronic exposure to a diet rich in fats changes the gastrointestinal milieu and alters responses to several signals involved in the control of food intake. Oleoylethanolamide (OEA) is a gut-derived satiety signal released from enterocytes upon the ingestion of dietary fats. The anorexigenic effect of OEA, which requir...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2014.04.039
更新日期：2014-09-01 00:00:00
abstract：:Within 3-4 weeks after spinal transection, morphine-induced antinociception, assessed with the tail flick reflex in rats, is profoundly reduced. The cause of this decrement is unknown. The present studies were conducted to determine whether this phenomenon reflects a general loss in opiate activity or a selective decl...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/s0031-9384(97)00240-0
更新日期：1997-10-01 00:00:00
abstract：:A wide range of data in the literature suggests that environmental enrichment has beneficial effects on various cognitive parameters in rodents. However, the magnitude of these effects and their persistence after the cessation of enrichment vary markedly across studies, with the use of different enrichment protocols p...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2007.09.009
更新日期：2008-01-28 00:00:00
abstract：:Midbrain raphe lesions induced aggressive behavior which was characterized by defensive aggression rather than offensive aggression in nature. Furthermore, raphe-lesioned rats exhibited muricide. This muricide included not only mouse-killing but also mouse-eating behavior. Both dorsal and medial raphe lesioned (dm-R) ...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(77)90166-4
更新日期：1977-07-01 00:00:00
abstract：:Memory and learning are impaired by imbalanced diet consumption. High-fat high-carbohydrate diet (HFCD) induces oxidative stress, which results in neuronal damage and interference with synaptic transmission; hence, a decline in cognitive function. Vitamin E is a fat soluble antioxidant that is believed to have positiv...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2013.06.011
更新日期：2013-07-02 00:00:00
abstract：:Bilateral intracranial injections of puromycin (10 micrograms/0.5 microliter vehicle) into the preoptic area (POA) of steroid-primed ovariectomized rats resulted in a significant decrease in the lordosis response when compared to saline treated controls. To determine the extent of puromycin diffusion after intracrania...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(84)90162-8
更新日期：1984-09-01 00:00:00
abstract：:The effects of central infusion of naloxone into the midbrain periaqueductal gray (PAG) upon predatory attack behavior in the cat were studied in 12 cats. Initially, quiet biting attack was elicited by electrical stimulation of sites within the lateral hypothalamus using monopolar electrodes. Then cannula-electrodes w...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(91)90337-n
更新日期：1991-06-01 00:00:00
abstract：:We have previously reported that the opioid receptor blocker, naloxone, is less effective in reducing palatable food intake in offspring exposed to a maternal cafeteria diet during the perinatal period, implicating a desensitization of the central opioid pathway in the programming of food preferences. The present stud...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2014.04.007
更新日期：2014-06-22 00:00:00
abstract：:This study analyzed in Balb/C mice the effects of individual housing on behavior, serum corticosterone and resistance to Ehrlich tumor growth. Mice (60 days old) were individually (IH) or grouped housed (G) (10-12 animals/cage) for 14-21 days. The 1st day of the housing condition was considered experimental day 1 (ED1...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2008.07.006
更新日期：2008-10-20 00:00:00
abstract：:Acute sport exercise leads to a strong stimulation of muscle tissue and a change in the organism energy demands. This study was designed to investigate the effect of oral melatonin supplementation on human physiological functions associated with acute exercise. Immune, endocrine and metabolic parameters were measured ...
journal_title：Physiology & behavior
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.physbeh.2011.12.015
更新日期：2012-03-20 00:00:00
abstract：:The influence of adrenalectomy (ADX) on the odor detection performance of male Long-Evans rats was assessed using high-precision olfactometry and a go/no-go operant signal detection task. Nonparametric signal detection measures of sensitivity and responsivity, as well as measures of S+ response latency, the number of ...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(91)90362-r
更新日期：1991-06-01 00:00:00
abstract：:Male rats were punished (0.6 mA/2 sec) for entering the shock compartment of a passive avoidance chamber. Twenty four hours later they were returned to the apparatus and confined to the grid floor of the shock compartment for 5 min (forced exposure). The bradycardic response found in control rats during forced exposur...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Multiple theta cells in the dentate granule cell area of the hippocampal formation of the freely moving rabbit were analyzed during three behavioral conditions: (1) voluntary motor patterns, termed Type 1 theta behaviors; (2) automatic motor patterns, termed Type 2 LIA behaviors; (3) alert immobility with presentation...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(83)90104-x
更新日期：1983-07-01 00:00:00
abstract：:Gas chromatography/mass spectrometry revealed the presence of both carbon disulfide (CS2) and carbonyl sulfide (COS) on rat breath. Behavioral experiments indicated that rats exposed to an unfamiliar diet moistened with CS2, like rats exposed to an unfamiliar diet placed on the fur of an anesthetized rat, subsequently...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(88)90285-5
更新日期：1988-01-01 00:00:00
abstract：:The coordination and regulation of electrical signals across excitable cells is a complex, dynamic phenomenon requiring, in part, the interaction of ion channels with cellular constituents. The intracellular loops or domains of many ion channel subunits have been shown to specifically bind other cellular components th...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/s0031-9384(02)00912-5
更新日期：2002-12-01 00:00:00
abstract：:Experiments were designed to distinguish between central and peripheral effects on temperature preference and body temperature of drugs injected intraperitoneally (IP) in infant mice ranging in age from 3 to 10 days postpartum. These compared a drug restricted to the periphery ("peripheral" drug) with a drug of simila...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(89)90257-6
更新日期：1989-08-01 00:00:00
abstract：:Caloric restriction in mice can trigger gorging behaviour, which is characterized by periods of excessive food ingestion in a short time. Animals that gorge are thought to have a reduced metabolism compared to those that nibble their food over a longer period and might therefore be more able to compensate for reduced ...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/j.physbeh.2007.07.005
更新日期：2007-12-05 00:00:00
abstract：:In the developing rodent, there is a period from about days 4 to 14 when the adrenal response to stress is either minimal or non-existent. This has been designated as the stress hyporesponsive period (SHRP). Numerous experiments have demonstrated that maternal factors are critical for the regulation of the pup's hypot...
journal_title：Physiology & behavior
pub_type： 杂志文章,评审
doi：10.1016/s0031-9384(01)00496-6
更新日期：2001-06-01 00:00:00
abstract：:The studies reported here addressed the question of whether the pungent principle in chilies, capsaicin, suppresses taste and flavor intensity. Over a period of several minutes, groups of frequent and infrequent eaters of chili repeatedly rated the taste and flavor intensities of sweet and sour solutions that also con...
journal_title：Physiology & behavior
pub_type： 杂志文章
doi：10.1016/0031-9384(95)02052-7
更新日期：1995-12-01 00:00:00
