Abstract：
OBJECTIVES:To report a single institution's experience using human papillomavirus (HPV) E6/E7 mRNA in-situ hybridization (mRNA ISH) for HPV detection in oropharyngeal squamous cell carcinoma (OPSCC). To review the literature on HPV detection methods. STUDY DESIGN:Retrospective chart review, literature review. SETTING:Tertiary care academic hospital. SUBJECTS AND METHODS:We conducted a retrospective chart review of 122 OPSCC biopsy specimens. mRNA ISH was performed on formalin-fixed paraffin-embedded (FFPE) tissue with a pool of 18 high risk HPV probes using an automated stainer; p16 immunohistochemistry (IHC) was also performed. We conducted a literature review on HPV detection methods including p16 IHC, mRNA ISH, DNA ISH, and PCR. RESULTS:In our cohort, mRNA ISH had a sensitivity and specificity of 100% and 100% with reference to p16 (100% concordance). 2-year OS was 87.5% vs. 94.5% for p16/HPV-negative vs. positive patients. 2-year DFS was 60.0% vs. 84.2%. On literature review, mRNA ISH demonstrated consistently high sensitivity and specificity ranging from 88-98% and 90-100% respectively. In comparison, the specificity of p16 was 85-95%. CONCLUSIONS:Our report supports the use of mRNA ISH for HPV detection in OPSCC and validates its feasibility using automated tissue staining methods on FFPE tissue. Our findings and literature review support that mRNA ISH may have superior specificity and be easier to interpret than p16. Further study on the prognostic value and cost-effectiveness of mRNA ISH is warranted and may establish this HPV detection method as the "gold standard."
journal_name
Am J Otolaryngoljournal_title
American journal of otolaryngologyauthors
Suresh K,Shah PV,Coates S,Alexiev BA,Samant Sdoi
10.1016/j.amjoto.2020.102782subject
Has Abstractpub_date
2021-01-01 00:00:00pages
102782issue
1eissn
0196-0709issn
1532-818Xpii
S0196-0709(20)30476-2journal_volume
42pub_type
杂志文章abstract：:Leiomyosarcoma of the paranasal sinuses is uncommon and has an aggressive clinical behavior. Only 28 cases have been described in the literature, and, of these, only 3 patients treated with surgery had a disease-free survival. In this report, we describe the clinical history of a 57-year-old woman with a leiomyosarcom...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1053/ajot.2002.30628
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:Laryngeal squamous cell carcinoma (LSCC) is strongly associated with tobacco use, but recent reports suggest an increasing incidence of LSCC in patients without traditional risk factors, suggesting an alternative etiology of tumorigenesis. The purpose of this study is to characterize this non-smoking populat...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2018.11.009
更新日期：2019-01-01 00:00:00
abstract：:Meniere's disease is a peripheral audiovestibular disorder characterized by vertigo, hearing loss, tinnitus, and aural fullness. Management of these symptoms includes medical and surgical treatment. Many patients with Meniere's disease can be managed using nonablative therapy, such as intratympanic steroids and endoly...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1016/j.amjoto.2020.102817
更新日期：2021-01-01 00:00:00
abstract：:Tuberculosis is a systemic disease and its occurrence in the larynx and in the oral cavity is well-documented in the literature. Tuberculosis of the larynx involves mainly the vocal folds and the ventricular band and is associated with pulmonary tuberculosis in 80% of cases. Isolated epiglottic tuberculosis has rarely...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1053/ajot.2002.123441
更新日期：2002-07-01 00:00:00
abstract：:Intense sounds were delivered to 11 subjects with normal hearing both before and during administration of standard doses of four nonsteroidal anti-inflammatory drugs. After four days of aspirin treatment (3.9 g daily), the subjects' resting hearing levels raised by about 10 dB. Administration of intense sounds that ha...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(84)80033-2
更新日期：1984-07-01 00:00:00
abstract：:Profound unilateral sensorineural hearing loss is an indication for the placement of a bone anchored hearing aid. In a few unfortunate patients who later develop contralateral hearing loss, a cochlear implant becomes a good option. We present our experience in these cases and discuss our technique for single stage con...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2015.05.003
更新日期：2015-09-01 00:00:00
abstract：:Exercise after vestibular injury is potentially an important modality in the recovery process. In this study, the effects of injury on the vestibular system were examined experimentally in cats. Data from healthy normal cats, labyrinthectomized cats, and labyrinthectomized cats treated with exercise were examined. Eva...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(82)80017-3
更新日期：1982-11-01 00:00:00
abstract：PURPOSE:The study objective is to evaluate the clinical features and outcomes of patients treated for head and neck malignant fibrous histiocytoma at a tertiary care medical facility. MATERIALS AND METHODS:This is a retrospective case series of 17 adult subjects with malignant fibrous histiocytoma of the head and neck...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2012.06.010
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:To investigate the combined use of a nanocarbon (NC) suspension and low-dose 99mTc-MIBI for parathyroid localization during surgery in patients with secondary hyperparathyroidism (sHPT). METHODS:Between March 2010 and September 2015, 40 patients with sHPT were enrolled in this study and were randomized to re...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.amjoto.2017.12.008
更新日期：2018-01-01 00:00:00
abstract：:A new mutation of the inner ear was discovered in golden hamsters raised in our laboratories. Although scanning electron microscopy showed a normal arrangement of individual stereocilia on the first row of outer hair cells, the entire bundle of stereocilia were irregular in orientation and scattered in several directi...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(88)80031-0
更新日期：1988-09-01 00:00:00
abstract：:Laryngeal sarcoma is a rare and potentially aggressive malignancy. In this case report, we present a 23year-old-male with four-years of progressive hoarseness who was found to have a large left paraglottic mass. A partial laryngectomy was successful at completely excising the lesion. Final pathology returned as alveol...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2017.01.017
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:To evaluate foot and ankle function in a series of patients undergoing fibula microvascular free tissue transfer. STUDY DESIGN:A nonrandomized, nonblinded analysis was performed using the ankle-hindfoot scale as well as radiographic analysis to determine donor site morbidity. RESULTS:Overall, foot ankle fun...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2005.07.003
更新日期：2006-01-01 00:00:00
abstract：:Recent technological advances and developments in surgical technique have made the craniocervical junction (CCJ) accessible through the transnasal surgical corridor. Endoscopic endonasal transclival and transodontoid approaches have been previously described in the literature. Traditionally, these approaches entail a ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2014.12.005
更新日期：2015-05-01 00:00:00
abstract：PURPOSE:The current loss to follow-up rate after failed newborn hearing screening (NBHS) is 34.4%. Previous studies have found that lack of parental and primary care provider (PCP) awareness of NBHS results are significant contributors to loss to follow-up. The objective of this study was to identify factors associated...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102614
更新日期：2020-01-01 00:00:00
abstract：:The management of a patient with traumatic disruption of the cricotracheal junction in an attempted suicide by hanging is described. Such injury is uncommon, and many patients die at the scene; detailed radiologic imaging is rare because of the urgency of airway management. The delayed complete disruption of the major...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2003.11.001
更新日期：2004-05-01 00:00:00
abstract：:The Asiatic Black Bear (Ursus thibetanus or Selenarctos thibetanus), also known as the Tibetan black bear, the Himalayan black bear, or the moon bear is a omnivorous mammal. This animal is declared threatened animal and rarely comes in human contact. Recent decrease in forest area has, however, increased the chances o...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2006.08.014
更新日期：2007-07-01 00:00:00
abstract：OBJECTIVES:We aim to present two cases of a retained insertion stylet from nasal feeding tube bridle systems. STUDY DESIGN:Case series of two patients. METHODS:Patients were evaluated from 8/2012 to 9/2013 for retained bridle system insertion stylet. RESULTS:Two patients presented with a retained stylet from nasal t...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2014.10.033
更新日期：2015-03-01 00:00:00
abstract：:Olfactory neuroblastoma is a rare malignant tumor of the nasal cavity arising from the olfactory neuroepithelium. It usually presents as a polypoid mass high in the nasal vault including the cribriform plate, superior turbinate, and superior portion of nasal septum. An 89-year-old man visited our office with symptoms ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2006.07.013
更新日期：2007-05-01 00:00:00
abstract：:A diagnosis of natural killer (NK)/T-cell lymphoma was clarified after repeated deep neck abscess in a 63-year-old man. The absolute number of lymphocytes was mildly decreased. Lymphopenia induced by latent malignant lymphoma in this case likely caused the immunodeficiency, which induced repeated infection in the head...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2008.05.004
更新日期：2009-07-01 00:00:00
abstract：:A regional random pattern flap is described which may be used to cover the mastoid area in cases in which the inferior auricle and adjacent tissue have been removed by trauma or surgery. The viability of this vascularized tissue offers a distinct advantage over skin grafting when protection of exposed facial nerve and...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(88)80032-2
更新日期：1988-09-01 00:00:00
abstract：:Malignant otitis externa is a severe and rare infection of the external acoustic meatus. Triphasic bone and (67)Ga scintigraphies are used to initial detect and follow-up the response of therapy. With single photon emission computed tomography/computed tomography images, the diagnostic sensitivity is higher. We presen...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2012.11.001
更新日期：2013-03-01 00:00:00
abstract：PURPOSE:HPV-related oropharyngeal squamous cell carcinomas clearly represent a growing entity in the head and neck with distinct carcinogenesis, clinico-pathological presentation and survival profile. We aimed to compare the HPV prevalence rates and clinico-pathological correlations obtained with three distinct commonl...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2013.08.007
更新日期：2014-01-01 00:00:00
abstract：PURPOSE:We treated allergic rhinitis with trichloroacetic acid (TCA) applied to the inferior turbinates. Further clinical evaluations were made to determine the effect of TCA application on symptoms of allergic rhinitis. We also report the results of measurement of the mucociliary transit time (saccharin time) and nasa...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2006.12.006
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVES:Several methods have been reported for inferior turbinate (IT) reduction. We describe office-based interstitial IT reduction using bipolar cautery and evaluate long-term efficacy. METHODS:Sixty patients with allergic and nonallergic rhinitis unresponsive to medical treatment underwent office IT reduction be...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102449
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:Bone-cement (BC) ossiculoplasty is one of the options to solve ossicular chain problems. Many authors reported successful results in the early or mid-follow-up period; however, there is no long-term result in the literature. We aim to evaluate long term results of BC ossiculoplasty. MATERIALS AND METHODS:Forty...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102620
更新日期：2020-01-01 00:00:00
abstract：:Herpes simplex virus infection of the larynx is an exceedingly rare clinical entity, most frequently reported in the pediatric population or in immunocompromised adults. We present a 62-year-old woman presented with neck pain, hoarseness, crepitus over the larynx, and what appeared to be a necrotic mass of the right t...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2012.11.006
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVES:Rare diseases are often poorly understood, and this study sought to investigate the incidence of a rare disease entity, basaloid squamous cell carcinoma (BSCC) of the oral cavity (OC) at a tertiary care medical center and to assess its clinical outcomes. METHODS:The aim of this study was to collect data in ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102645
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:The purpose of this retrospective cohort study was to determine whether there is a difference in the sensitivity of chest computed tomography (CT) versus 18F-fluorodeoxyglucose positron emission tomography with low-dose nonenhanced CT (18F-FDG PET/CT or PET/CT) in the detection of distant metastases in head and...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102662
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:To investigate objective measures that could increase the positive predictive value of computed tomography (CT) in diagnosing deep neck space infections (DNSIs). METHODS:A retrospective analysis of patients surgically treated at a tertiary care hospital for DNSIs for more than 2 years were reviewed. Patients w...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2005.11.008
更新日期：2006-07-01 00:00:00
abstract：PURPOSE:To further enhance and assess the ability to characterize middle ear effusion (MEE) using non-invasive ultrasound technology. MATERIALS AND METHODS:This is a prospective unblinded comparison study. Fifty-six children between the ages of 6 months and 17 years scheduled to undergo bilateral myringotomy with pres...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2012.08.005
更新日期：2013-01-01 00:00:00
