Abstract:
BACKGROUND:Invariant natural killer T cells (iNKT cells) may suppress graft-versus-host disease (GVHD) after allogeneic transplantation. The purpose of this study was to investigate the therapeutic potential of iNKT cells from major histocompatibility complex (MHC)-mismatched donors for preventing GVHD after allogeneic bone marrow transplantation (BMT). STUDY DESIGN AND METHODS:In vitro, mouse iNKT cells were expanded with alpha-galactosylceramide and interleukin (IL)-2 treatment. In the NKT-treated group, lethally irradiated DBA/2(H-2K(d)) mice were adoptively transferred with expanded iNKT, bone marrow (BM), and spleen cells (SCs) from C57BL/6 (H-2K(b)) mice. Recipients in the control group were transferred only BM and SCs. The two groups were compared in survival, weight, histopathologic specimens, and serum cytokine analysis. RESULTS:In the iNKT-treated group, 80% of mice survived past Day 60 after BMT, but all died within 38 days in the control group. The mice treated with iNKT did not exhibit signs of GVHD after Day 42 except for a change in fur color. There were higher IL-4 levels by Day 7 in serum of mice that received iNKT compared to those without iNKT treatment, while the interferon-gamma levels showed no significant difference between two groups. Levels of IL-2 and IL-5 increased by Day 21 only in iNKT-treated mice. CONCLUSION:The results suggest that donor iNKT cells could alleviate GVHD symptoms and prolong survival after MHC-mismatched allogeneic BMT, which may be associated with the maintenance in IL-4 levels. These findings indicate that the therapy based on iNKT cells from MHC-mismatched donors has great potential in protection against GVHD after allogeneic hematopoietic stem cell transplantation.
journal_name
Transfusionjournal_title
Transfusionauthors
Yang J,Gao L,Liu Y,Ren Y,Xie R,Fan H,Qian Kdoi
10.1111/j.1537-2995.2009.02395.xsubject
Has Abstractpub_date
2010-02-01 00:00:00pages
407-17issue
2eissn
0041-1132issn
1537-2995pii
TRF2395journal_volume
50pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Posttransfusion graft-versus-host disease (PT-GVHD) is underdiagnosed and underreported. Risk factors predisposing to PT-GVHD in newborn recipients, the clinical manifestations of the disease in newborns, and its mechanism are not well characterized. STUDY DESIGN AND METHODS:A literature review identified 2...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1046/j.1537-2995.1996.36296181922.x
更新日期:1996-02-01 00:00:00
abstract::Monitoring of platelet counts 1 hour after transfusion has become standard practice in most centers. In this study, platelet counts obtained 10 and 60 minutes after 48 platelet transfusions were compared. There was a close, linear relationship (r = 0.98) between these values over a wide range of posttransfusion counts...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28188127957.x
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND:Effective risk communication in transfusion medicine is important for health-care consumers, but understanding the numerical magnitude of risks can be difficult. The objective of this study was to determine the effect of a visual risk communication tool on the knowledge and perception of transfusion risk. S...
journal_title:Transfusion
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1537-2995.2003.00402.x
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:Bacterial contamination or platelet (PLT) metabolism can change the pH of stored PLT concentrates (PCs). Measurement of pH for quality control is currently done on a limited basis. An easy noninvasive method was developed to obtain sequential pH measurements over time, without risking contamination and/or co...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02727.x
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Blood donor parvovirus B19 DNA prevalence with sensitive nucleic acid test assays has recently been demonstrated to be higher than that found with assays designed to detect high viral titers in the plasma manufacturing sector. STUDY DESIGN AND METHODS:Stored plasma aliquots from 5020 donations collected bet...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01341.x
更新日期:2007-10-01 00:00:00
abstract::A stable mouse hybridoma line secreting monoclonal anti-C3d antibody (NBTS/BRIC 8) was produced. Ascitic fluid containing BRIC 8 can be used at a final dilution of 1 to 5000 to provide an excellent antiglobulin reagent when mixed with a conventional polyclonal rabbit anti-human IgG serum. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25385219912.x
更新日期:1985-05-01 00:00:00
abstract:BACKGROUND:Anti-Tc(a) detects a high-incidence antigen in the Cromer blood group system. Cromer system antibodies have not usually been associated with hemolytic transfusion reactions or hemolytic disease of the newborn. CASE REPORT:Anti-Tc(a) (initially identified in the patient's serum in 1982) was not detected when...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1999.39090948.x
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Very preterm newborns receive up to three to five red blood cell (RBC) transfusions, often early, after birth. Despite awareness of the association of transfusion with increases in cytokines and markers of endothelial activation, research has focused on single transfusions weeks after birth. With pathophysio...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15952
更新日期:2020-07-25 00:00:00
abstract:BACKGROUND:Washing of red blood cell concentrates (RCCs) is required for potassium-sensitive transfusion recipients, including neonates in need of large-volume transfusions. When open, nonsterile washing systems are used, postwash outdate time is limited to 24 hours, often leading to problems providing the component to...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12170
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND:Donor granulocyte concentrates are routinely administered to patients with granulocyte function defects or transient neutropenia and (risk of) bacterial or fungal exacerbations, despite lack of definitive clinical proof for patient-relevant outcome improvement. Granulocytes are collected by apheresis from he...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12197
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Bartonella spp. are neglected fastidious Gram-negative bacilli. We isolated Bartonella henselae from 1.2% of 500 studied blood donors and demonstrated that the bacteria remain viable in red blood cell units after 35 days of experimental infection. Now, we aim to evaluate the possibility of B. henselae transm...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13545
更新日期:2016-06-01 00:00:00
abstract::Because there may be inadequate dextrose in the newly licensed CPD-adenine for five or six weeks storage of high hematocrit red blood cells, this laboratory has examined some alternate sugars for their ability to maintain red blood cell metabolism during storage. In the current study, dextrose and fructose were studie...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1980.20180125025.x
更新日期:1980-01-01 00:00:00
abstract::Current standards for the preparation of factor VIII (FVIII) concentrates from human plasma recommend separation of plasma from red cells (RBCs) within 6 hours of blood donation, thereby reducing the volume of plasma from donated whole blood available for processing to FVIII concentrate. The decay of FVIII clotting ac...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28689059033.x
更新日期:1988-11-01 00:00:00
abstract::Donation willingness is generally high but registering with donor centers lags behind. We need to understand why and when during the life course which groups of individuals are motivated to donate. With this knowledge, we can design targeted recruitment and retention strategies and improve evidence-based donor managem...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.15268
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to evaluate an iodine-based method of activating potentially harmful viruses that might be found in normal human plasma. STUDY DESIGN AND METHODS:A procedure has been developed for inactivating lipid-enveloped and non-lipid-enveloped model viruses in normal human plasma by usin...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34494233580.x
更新日期:1994-04-01 00:00:00
abstract::A system of weekly turnover of blood inventory has been shown capable of reducing outdating in small hospitals. One problem is ensuring the return of unused blood at the end of the first week. Outdating becomes intolerable if the blood is returned to the blood center after two weeks. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1979.19680104100.x
更新日期:1979-11-01 00:00:00
abstract:BACKGROUND:The effect on component wastage after enhancing the clinician's ability to order blood products in the computerized physician order entry (CPOE) system was investigated in a multihospital network. METHODS:A novel field was added to the CPOE of eight hospitals within a health care system allowing the physici...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13399
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Platelets (PLTs) stored in additive solutions (PASs) may reduce the risk of several plasma-associated adverse transfusion reactions such as allergic reactions and potentially transfusion-associated lung injury. The objective of this study was to determine the in vitro characteristics and the in vivo radiolab...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2012.03841.x
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:A novel WBC-reduction in-line whole-blood (WB) filter that does not retain platelets was evaluated to assess the filtration performance and, after processing WB by the platelet-rich plasma (PRP) method, to analyze the storage quality of filtered platelet concentrate (PC) units. STUDY DESIGN AND METHODS:To a...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.0041-1132.2003.00587.x
更新日期:2003-12-01 00:00:00
abstract:BACKGROUND:Guidelines for red blood cell (RBC) transfusions exist; however, transfusion practices vary among centers. This study aimed to analyze transfusion practices and the impact of patients and institutional characteristics on the indications of RBC transfusions in preterm infants. STUDY DESIGN AND METHODS:RBC tr...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1537-2995.2009.02373.x
更新日期:2010-01-01 00:00:00
abstract::As the number of successful marrow transplants has increased, the lack of HLA-identical sibling donors for 60 to 70 percent of transplant candidates has become a serious problem. Pilot studies established that marrow transplantation between phenotypically HLA-identical, unrelated individuals can be accomplished succes...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33793325052.x
更新日期:1993-07-01 00:00:00
abstract:BACKGROUND:Transfusion-transmitted babesiosis (TTB) has been rapidly increasing in incidence since the beginning of the 21st century. Asymptomatic individuals with Babesia infection are able to donate blood in the United States because of the lack of specific blood donation testing. Blood products collected in Babesia-...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14246
更新日期:2017-10-01 00:00:00
abstract::ALX148, a novel CD47 blocking agent, is in clinical development for the treatment of advanced solid tumors and lymphoma. Because CD47 is highly expressed on red blood cells (RBCs), its therapeutic blockade can potentially interfere with pretransfusion compatibility testing. This study describes the interference of ALX...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16009
更新日期:2020-08-20 00:00:00
abstract::In spite of growing awareness of the potential risks associated with transfusion, the number of platelet units transfused in the United States continues to increase each year. There is a growing interest in ensuring that all transfusions are administered for appropriate reasons. Prospective review of requests for tran...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31791368335.x
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:The diagnosis of immune thrombocytopenic purpura (ITP) is a diagnosis of exclusion, as stated by international guidelines. Nevertheless, the assessment of platelet (PLT) antibodies has been reported as helpful for the diagnosis and the follow-up of ITP patients. PLT antibodies are detected by highly speciali...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01556.x
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:Techniques for banking cord blood units (CBUs) as source for hematopoietic stem cell transplantation have been developed over the past 20 years, aimed to improve laboratory efficiency without altering the biologic properties of the graft. A large-scale, registry-based assessment of the impact of the banking ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13661
更新日期:2016-08-01 00:00:00
abstract::This review considers the various methods currently in use, or under investigation, for the storage of platelets intended for use in the treatment of active hemorrhage. The current standard practice of storing platelets at room temperature (RT) (20°C-24°C) optimizes circulating time, but at the expense of hemostatic f...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.13504
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:Reported hepatitis E virus (HEV) antibody assay performance characteristics are variable. Using a subset of surplus US blood donation samples, we compared assays for detecting anti-HEV immunoglobulin M (Ig)M and IgG or total anti-HEV antibodies. STUDY DESIGN AND METHODS:Samples from 5040 random blood donati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14553
更新日期:2018-05-01 00:00:00
abstract::In this study we tested the seeds of uncultivated plants for lectin activity. Extracts prepared from the seeds of 167 forb species were tested against human red cell samples. Extracts of 54 species agglutinated unmodified or enzyme-modified red cells. In nine species, the hemagglutinating lectin activity could be neut...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23684074276.x
更新日期:1983-11-01 00:00:00
abstract:BACKGROUND:Human infections with simian foamy viruses (SFVs) have been reported after occupational and nonoccupational exposure to infected animals and their tissues, blood, and body fluids, although there is no evidence for human-to-human transmission. We previously demonstrated SFV transmission in monkeys by blood tr...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02372.x
更新日期:2010-01-01 00:00:00