Abstract：
OBJECTIVE:There is an increasing interest concerning the contribution of astrocytes to the intrinsic bioremediation of ischemic brain injury. The aim of this work was to disclose the effects and mechanism of dexmedetomidine (DEX) on the apoptosis of astrocytes under oxygen glucose deprivation (OGD) condition. METHODS:Primary cultured astrocytes separated from Sprague-Dawley (SD) rats were subjected to OGD treatment. Astrocytes were transfected with si-JMJD3 or pcDNA3.1-JMJD3 and then treated with DEX or JAK/STAT inhibitor (WP1066) before cell apoptosis was detected by TUNEL apoptosis kit. Western blot was applied to assess the level of apoptosis-related proteins Caspase-3, Bax and Bcl-2. Astrocyte cell viability was assessed by measuring the lactate dehydrogenase (LDH) level using a LDH assay kit. RESULTS:Astrocytes received OGD treatment had increased LDH and elevated apoptotic rate (P < 0.05). DEX could suppress OGD induced cytotoxic effect on astrocytes, as evidenced by decreased LDH release and suppressed cell apoptosis rate (P < 0.05). Meanwhile, DEX and WP1066 treatment were also found to inhibit the phosphorylation level of STAT1 and STAT3 (P < 0.05), indicating the DEX could suppress the activation of JAK/STAT signal pathway. JMJD3 overexpression in astrocytes could suppress the anti-apoptotic function of WP1066 in OGD treated astrocytes and hamper the protective effect of DEX in cell apoptosis (P < 0.05), suggesting that DEX and JAK/STAT signal pathway inhibits OGD induced apoptosis in astrocytes by down-regulating JMJD3. CONCLUSION:DEX protects astrocytes against apoptosis via inhibiting JAK2/STAT3 signal pathway and downregulating JMJD3 expression in vitro.
journal_name
Brain Resjournal_title
Brain researchauthors
Feng P,Zhang A,Su M,Cai H,Wang X,Zhang Ydoi
10.1016/j.brainres.2020.147141subject
Has Abstractpub_date
2021-01-01 00:00:00pages
147141eissn
0006-8993issn
1872-6240pii
S0006-8993(20)30499-6journal_volume
1750pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Endothelins are potent vasoconstrictors and signaling molecules. Their effects are broad, impacting processes ranging from neurovascular and cardiovascular health to cell migration and survival. In stroke, traumatic brain injury or subarachnoid hemorrhage, endothelin-1 (ET-1) is induced resulting in cerebral vasospasm...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.09.022
更新日期：2014-11-07 00:00:00
abstract：:Administration of non-competitive N-methyl-D-aspartate (NMDA) receptor antagonists (e.g. phencyclidine, MK-801) has been shown to elicit behavioral abnormalities related to symptoms of schizophrenia, such as spontaneous locomotor activity and impaired sensorimotor gating, as represented by deficits of prepulse inhibit...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.07.013
更新日期：2010-09-17 00:00:00
abstract：:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes a disruption of nigrostriatal dopaminergic function which resembles parkinsonism. On the cellular level, the disruption involves a Ca2+ independent release of dopamine, depletion of nigral and striatal dopamine, and the death of dopaminergic neurons. We have p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91035-y
更新日期：1991-02-15 00:00:00
abstract：:(1) Spontaneous barbiturate spindles were recorded from the primary and secondary somatosensory cortex. The recordings were concentrated to areas surrounding several reference loci. The recording sites producing the maximal response evoked by stimulation of an exposed nerve in a contralateral limb were used as referen...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)90984-7
更新日期：1976-01-09 00:00:00
abstract：:Mechanical hyperalgesia is an early symptom of diabetic neuropathy. To evaluate the mechanisms underlying this symptom, it was studied and compared in rat models of systemic and local hyperglycemia. Systemic hyperglycemia was induced by a single injection of streptozotocin (STZ, 50 mg/kg). Local hyperglycemia either i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03828-3
更新日期：2003-01-17 00:00:00
abstract：:This study was designed to assess effects of insulin-induced hypoglycemia on plasma and cerebrospinal fluid (CSF) levels of immunoreactive (ir) beta-endorphins, adrenocorticotropin (ACTH), cortisol, norepinephrine, insulin, and glucose in the conscious, overnight fasted dog. Dogs received either an intravenous infusio...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90475-1
更新日期：1988-08-23 00:00:00
abstract：:Cerebral amyloid angiopathy (CAA) might disturb the sensitive mechanism of cerebral pressure autoregulation. This study examines whether dynamic cerebral autoregulation (CA) is impaired in the posterior or anterior circulation of CAA patients. Fifteen patients with known CAA on magnetic resonance imaging (MRI) and 14 ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2019.04.014
更新日期：2019-08-15 00:00:00
abstract：:In the present study we have examined the effects of changes in potassium and calcium concentration on the early adaptation of the slowly adapting stretch receptor of the crayfish using intracellular recordings including the potential clamp technique. This was because previous studies had suggested that the early adap...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90409-3
更新日期：1985-06-10 00:00:00
abstract：:The aim of this work was to study whether the vestibular afferent fibers establish direct connections with the motoneurons of glossopharyngeal and vagus nerves of the frog, Rana esculenta. In anaesthetized animals the vestibulocochlear nerve and the common root of glossopharyngeal-vagus and accessory (IX-X-XI) nerves ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.06.048
更新日期：2009-08-25 00:00:00
abstract：:Nitric oxide (NO) can have opposite effects on peripheral sensory neuron sensitivity depending on the concentration and source of NO, and the experimental setting. The aim of this study was to determine the role of endogenous NO production in the regulation of mechanosensitive Ca(2+) influx of dorsal root ganglion (DR...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02407-6
更新日期：2001-06-08 00:00:00
abstract：:Converging evidence suggests that compromised motor abilities in hemiparetic cerebral palsy are not solely due to impairments in motor execution, but are also related to deficits in action planning. The present study had two aims. First, we compared grip planning in a sequential task between participants with left-sid...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.12.074
更新日期：2009-03-19 00:00:00
abstract：:To assess the role of androgen-dependent brain vasopressinergic transmission in the modulation of social recognition, castrated male rats which are deficient in vasopressin were implanted intracerebroventricularly with an Accurel collodion mini-device containing 10 micrograms vasopressin (AVP) whereas intact male rats...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90480-w
更新日期：1992-02-14 00:00:00
abstract：:The recently developed technique of in vivo dialysis has permitted us to make direct measurements of serotonin release in a specific region of the spinal cord and to relate this to changes in blood pressure elicited by chemical stimulation of the brainstem. In the present experiments we have shown that chemical stimul...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91318-1
更新日期：1986-02-26 00:00:00
abstract：:Neurotensin (NT) is an endogenous brain tridecapeptide for which high affinity binding sites exist in the central nervous system. We have investigated the effects of NT incubation with rat neostriatal slices on calcium/calmodulin (Ca/CaM)-dependent protein phosphorylation. Slices were incubated with NT (5 or 50 nM) fo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91311-n
更新日期：1991-04-05 00:00:00
abstract：:Contradictory results have been reported with regard to the effects of various models of hepatic encephalopathy on the blood-brain barrier (BBB) permeability, which may be due partly to the use of brain concentrations of BBB markers without attention to their peripheral pharmacokinetics. The purpose of the current stu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.07.040
更新日期：2013-09-19 00:00:00
abstract：:Previous studies have demonstrated that food restriction during lactation extends the length of lactational infertility in rats. In order to determine whether Neuropeptide Y (NPY) plays a role in the increased length of lactational infertility seen in food-restricted rats, NPY immunoreactivity in the arcuate nucleus o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00351-x
更新日期：1997-07-04 00:00:00
abstract：:The acquisition and retention of tolerance to the antinociceptive effect of supraspinal morphine on the tail withdrawal reflex was assessed in rats implanted with unilateral cannulae in the periaqueductal gray (PAG). Development of tolerance to daily microinjections of morphine was indicated by the return of the tail ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91476-4
更新日期：1987-10-27 00:00:00
abstract：:Indirect immunofluorescence cytochemistry of ovine corticotropin releasing factor (oCRF) was performed by use of an antiserum raised against a conjugate of oCRF and bovine thyroglobulin. The staining intensity was quantitated by use of an automated microfluorimeter. In cryostat sections of formaldehyde fixed oCRF cont...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91458-6
更新日期：1984-02-13 00:00:00
abstract：:Based on pharmacological evidence that inhibitory amino acids mediate vaginocervical mechano-stimulation produced analgesia (VSPA), we hypothesized that inhibitory amino acids would be released endogenously in the spinal cord in response to vaginocervical mechano-stimulation (VS). This hypothesis was tested by HPLC an...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90117-6
更新日期：1993-09-10 00:00:00
abstract：:Chronic administration of caffeine (s.c. for a period of 14 days in escalating doses of 10-70 mg/kg) increased the sensitivity of rat cerebral cortical neurons to the inhibitory action of microiontophoretically applied adenosine. The sensitivity of spontaneously firing rat cerebral cortical neurons in caffeine-treated...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91722-s
更新日期：1990-06-18 00:00:00
abstract：:Choline is an essential nutrient whose availability during the second half of gestation produces long-lasting cognitive effects. Rats that obtain supplemental choline during embryonic day (E) 11-17 have enhanced depolarization-evoked acetylcholine (ACh) release from hippocampal slices, whereas choline deficiency durin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.08.046
更新日期：2008-10-27 00:00:00
abstract：:In adolescence, gender differences in rates of affective disorders emerge. For both adolescent boys and girls, peer relationships are the primary source of life stressors though adolescent girls are more sensitive to such stressors. Social stressors are also powerful stressors for non-human social species like rodents...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.04.068
更新日期：2010-07-09 00:00:00
abstract：:Monoclonal antibody N210 (mabN210) recognizes the 210 kdalton neurofilament protein in mammals and gives characteristic immunocytochemical staining of neurofilament-rich processes. For example, in the cerebellum it recognizes myelinated axons and the calyx formed by basket cell axon collaterals. The distribution of ma...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90937-6
更新日期：1986-07-23 00:00:00
abstract：:The effects of natural (mechanical and gustatory) stimulation of the nasopharynx or electrical stimulation of the pharyngeal branch of the glossopharyngeal (PH-IXth) nerve on the changes in heart rate (HR) and arterial blood pressure (BP) were investigated in paralyzed and anesthetized rats. Afferent responses in the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91614-x
更新日期：1993-08-13 00:00:00
abstract：:Correlative light and electron microscopic immunohistochemical methods were adapted for a descriptive analysis of the normal time course and pattern of expression and intraneuronal localization of the enzyme glutamic acid decarboxylase (GAD) in the neostriatum (Ns) of fetal, postnatal and adult cats. The differentiati...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90155-6
更新日期：1987-06-01 00:00:00
abstract：:The effect of MK-801 on striatal dopamine (DA) release was investigated by using an in vivo microdialysis technique in the freely moving rat. Systemic injection of MK-801 (0.25, 0.5, 1, 2 mg/kg, i.p.) reduced the extracellular level of DA significantly and produced no change in the level of 3,4-dihydroxyphenylacetic a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90197-j
更新日期：1990-09-24 00:00:00
abstract：:Dural afferent neurons are implicated in primary headaches including migraine. Although a significant portion of primary afferent neurons innervating the dura are myelinated A-type neurons, previous electrophysiological studies have primarily characterized the functional properties of small-sized C-type sensory neuron...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.06.007
更新日期：2018-10-01 00:00:00
abstract：:The taste reactivity test described in the preceding paper was used to begin determining the capacity of brain stem structures to execute and regulate ingestive behavior. Both chronic thalamic and chronic decerebrate rat preparations were examined repeatedly, and their gustatory mimetic responses compared through fram...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90569-3
更新日期：1978-03-24 00:00:00
abstract：:Noradrenergic, serotoninergic and peptidergic nerves have been demonstrated in perivascular plexuses of vasa nervorum of sympathetic, parasympathetic and somatic nerve trunks. 5-Hydroxytryptamine-, vasoactive intestinal polypeptide- and substance P-containing fibers were found by immunohistochemistry to variable exten...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90344-5
更新日期：1984-06-25 00:00:00
abstract：:The distribution of melanin-concentrating hormone-like immunoreactivity (MCH-LI) in 41 microdissected brain and spinal cord regions was determined using radioimmunoassay with antibodies to salmon MCH. The highest concentration of MCH-LI was detected just ventral to the zona incerta (subzona incerta) (2923.2 fmol/mg pr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90337-9
更新日期：1986-05-14 00:00:00
