Epidemiology of rabies virus variants. Differentiation using monoclonal antibodies and discriminant analysis.

Abstract:

:Rabies virus was isolated by cell culture from the brains of 104 confirmed rabies cases diagnosed by the fluorescent-antibody staining technique in the United States during 1974-1984. Eighty-seven isolates were obtained from wild-life species, 10 from humans, and seven from domestic animals. These isolates were tested in virus neutralization and immunofluorescence assays using a panel of 34 monoclonal antibodies specific for rabies virus nucleocapsid protein, 44 monoclonal antibodies specific for rabies virus glycoprotein, and two monoclonal antibodies specific for rabies virus nucleocapsid-associated phosphoprotein. Using discriminant analysis, a distinctly different reactivity pattern was revealed between virus isolates from terrestrial (raccoon, skunk) and nonterrestrial (bat) reservoir hosts. The usefulness of this approach for studying the epidemiology of rabies and for predicting the source of infection when this information is unknown is discussed.

journal_name

Am J Epidemiol

authors

Rupprecht CE,Glickman LT,Spencer PA,Wiktor TJ

doi

10.1093/aje/126.2.298

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

298-309

issue

2

eissn

0002-9262

issn

1476-6256

journal_volume

126

pub_type

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