Molecular and functional profiling of human islets: from heterogeneity to human phenotypes.

Abstract:

:Efforts to phenotype pancreatic islets have contributed tremendously to our present understanding of endocrine function and diabetes. A continued evolution in approaches to study islet physiology is important given the need to establish reference points for mature islet functionality, understanding biological variation amongst individuals and cells, and the ongoing appreciation of the role for islets in diabetes susceptibility. Recent efforts in islet biology have focused on technological improvements in imaging, molecular profiling and data analysis, along with a push for enhanced transparency and reporting. The integration of these approaches within a classical islet physiology framework, and approaches to link these data with in vivo human phenotypes, will be critical as we move towards a better understanding of islet function in health and disease. Here we discuss what we feel are important issues and useful approaches to consider as we move forward as a field in islet and beta cell phenotyping. Graphical abstract.

journal_name

Diabetologia

journal_title

Diabetologia

authors

Arrojo E Drigo R,Roy B,MacDonald PE

doi

10.1007/s00125-020-05159-8

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

2095-2101

issue

10

eissn

0012-186X

issn

1432-0428

pii

10.1007/s00125-020-05159-8

journal_volume

63

pub_type

杂志文章,评审
  • Infection of cultured human pancreatic B cells with reovirus type 3.

    abstract::The capacity of reovirus type 3 to infect insulin-producing B cells was studied in human pancreatic cell cultures. Antibody to reovirus was labelled with fluorescein isothiocyanate and antibody to insulin was labelled with tetramethyl rhodamine isothiocyanate. By using a double-labelled immunofluorescent antibody tech...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00253408

    authors: Yoon JW,Selvaggio S,Onodera T,Wheeler J,Jenson AB

    更新日期:1981-04-01 00:00:00

  • Coffee consumption and risk of total and cardiovascular mortality among patients with type 2 diabetes.

    abstract:AIMS/HYPOTHESIS:Higher habitual coffee drinking has been associated with a lower risk of developing type 2 diabetes. The relation between coffee consumption and risk of cardiovascular disease (CVD) has been examined in many studies, but the issue remains controversial. This study was designed to assess the association ...

    journal_title:Diabetologia

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s00125-006-0435-9

    authors: Bidel S,Hu G,Qiao Q,Jousilahti P,Antikainen R,Tuomilehto J

    更新日期:2006-11-01 00:00:00

  • Mitochondrial regulation of insulin production in rat pancreatic islets.

    abstract:AIMS/HYPOTHESIS:The study was designed to identify the key metabolic signals of glucose-stimulated proinsulin gene transcription and translation, focusing on the mechanism of succinate stimulation of insulin production. METHODS:Wistar rat islets were incubated in 3.3 mmol/l glucose with and without esters of different...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-005-1811-6

    authors: Leibowitz G,Khaldi MZ,Shauer A,Parnes M,Oprescu AI,Cerasi E,Jonas JC,Kaiser N

    更新日期:2005-08-01 00:00:00

  • Do insulinotropic glucose-lowering drugs do more harm than good? The hypersecretion hypothesis revisited.

    abstract::Significant progress has been made in recent years in the characterisation of the signal pathways of beta cell dysfunction and death in the pathogenesis of type 2 diabetes. Glucolipotoxicity acts as an exogenous factor whereas oxidative stress and endoplasmic reticulum stress may result from the processes of signal re...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-010-1839-0

    authors: Rustenbeck I,Baltrusch S,Tiedge M

    更新日期:2010-10-01 00:00:00

  • Risk of cancer in patients on insulin glargine and other insulin analogues in comparison with those on human insulin: results from a large population-based follow-up study.

    abstract:AIMS/HYPOTHESIS:Several publications suggest an association between certain types of insulin and cancer, but with conflicting results. We investigated whether insulin glargine (A21Gly,B31Arg,B32Arg human insulin) is associated with an increased risk of cancer in a large population-based cohort study. METHODS:Data for ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-011-2312-4

    authors: Ruiter R,Visser LE,van Herk-Sukel MP,Coebergh JW,Haak HR,Geelhoed-Duijvestijn PH,Straus SM,Herings RM,Stricker BH

    更新日期:2012-01-01 00:00:00

  • Insulin-induced activation of phosphoinositide 3-kinase in Fao cells.

    abstract::Phosphoinositide 3-kinase (PI3-kinase) plays a crucial role in insulin signal transduction. We studied the molecular mechanism of the insulin-induced activation of PI3-kinase in rat hepatoma Fao cells using an antibody against the 110-kDa catalytic subunit (p110) and two against the 85-kDa regulatory subunit (p85 alph...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00403297

    authors: Hayashi T,Okamoto M,Yoshimasa Y,Inoue G,Yamada K,Kono S,Shigemoto M,Suga J,Kuzuya H,Nakao K

    更新日期:1996-05-01 00:00:00

  • Lipolysis and cyclic AMP levels in epididymal adipose tissue of obese-hyperglycaemic mice.

    abstract::Glycerol release from epididymal fat fragments of young adult (3-month old) ob/ob mice was three times lower than normal, on a tissue weight basis. Dose-response curves in response to isoproterenol and ACTH-(1--24) indicated that the capacity of the lipolytic process was reduced. However, the sensitivity to both hormo...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF01236307

    authors: Dehaye JP,Winand J,Christophe J

    更新日期:1977-12-01 00:00:00

  • Insulitis in human diabetes: a histological evaluation of donor pancreases.

    abstract:AIMS/HYPOTHESIS:According to the consensus criteria developed for type 1 diabetes, an individual can be diagnosed with insulitis when ≥ 15 CD45+ cells are found within the parenchyma or in the islet-exocrine interface in ≥ 3 islets. The aim of this study was to determine the frequency of individuals with type 2 diabete...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-016-4140-z

    authors: Lundberg M,Seiron P,Ingvast S,Korsgren O,Skog O

    更新日期:2017-02-01 00:00:00

  • Visceral fat and beta cell function in non-diabetic humans.

    abstract:AIMS/HYPOTHESIS:Preferential visceral adipose tissue (VAT) accumulation has been clearly associated with insulin resistance. In contrast, the impact of visceral obesity on beta cell function is controversial. METHODS:In 62 non-diabetic women and men (age 24-69 years, BMI 21-39 kg/m2), we measured VAT and subcutaneous ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-005-1891-3

    authors: Gastaldelli A,Sironi AM,Ciociaro D,Positano V,Buzzigoli E,Giannessi D,Lombardi M,Mari A,Ferrannini E

    更新日期:2005-10-01 00:00:00

  • High-dose thiamine therapy counters dyslipidaemia in streptozotocin-induced diabetic rats.

    abstract:AIMS/HYPOTHESIS:Cardiovascular disease in diabetes is linked to increased risk of atherosclerosis, increased levels of triglyceride-rich lipoproteins and enhanced hepatic lipogenesis. The hepatic hexosamine pathway has been implicated in signalling for de novo lipogenesis by the liver. In this study, we assessed if dec...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-004-1582-5

    authors: Babaei-Jadidi R,Karachalias N,Kupich C,Ahmed N,Thornalley PJ

    更新日期:2004-12-01 00:00:00

  • Characterisation of insulin-like growth factor I receptor in skeletal muscles of normal and insulin resistant subjects.

    abstract::The insulin-like growth factor I receptor and the activity of its associated tyrosine kinase activity were characterised in wheat germ agglutinin extracts from skeletal muscle biopsies from nine control and ten obese Type 2 (non-insulin-dependent) diabetic subjects, who had marked peripheral in vivo insulin resistance...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00265369

    authors: Livingston N,Pollare T,Lithell H,Arner P

    更新日期:1988-12-01 00:00:00

  • Urinary podocalyxin is an early marker for podocyte injury in patients with diabetes: establishment of a highly sensitive ELISA to detect urinary podocalyxin.

    abstract:AIMS/OBJECTIVE:Nephropathy, a major complication of diabetes, is the leading cause of end-stage renal disease. Recent studies have demonstrated that podocyte injury is involved in the onset of and progression to renal insufficiency. Here, we describe a novel, highly sensitive ELISA for detecting urinary podocalyxin, a ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-012-2661-7

    authors: Hara M,Yamagata K,Tomino Y,Saito A,Hirayama Y,Ogasawara S,Kurosawa H,Sekine S,Yan K

    更新日期:2012-11-01 00:00:00

  • Safety of high-dose nicotinamide: a review.

    abstract::Nicotinamide, the amide derivative of nicotinic acid, has over the past forty years been given at high doses for a variety of therapeutic applications. It is currently in trial as a potential means of preventing the onset of Type I (insulin-dependent) diabetes mellitus in high-risk, first-degree relatives. Nicotinamid...

    journal_title:Diabetologia

    pub_type: 杂志文章,评审

    doi:10.1007/s001250051536

    authors: Knip M,Douek IF,Moore WP,Gillmor HA,McLean AE,Bingley PJ,Gale EA,European Nicotinamide Diabetes Intervention Trial Group.

    更新日期:2000-11-01 00:00:00

  • Adiposity, inflammation and hyperglycaemia in rural and urban Indian men: Coronary Risk of Insulin Sensitivity in Indian Subjects (CRISIS) Study.

    abstract:AIMS/HYPOTHESIS:The aim of this study was to investigate whether the higher prevalence of insulin resistance and glucose intolerance in urban compared with rural Indian men is related to their higher adiposity (percentage body fat) and the associated inflammatory state. METHODS:We studied 149 rural, 142 urban slum and...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-007-0847-1

    authors: Yajnik CS,Joglekar CV,Lubree HG,Rege SS,Naik SS,Bhat DS,Uradey B,Raut KN,Shetty P,Yudkin JS

    更新日期:2008-01-01 00:00:00

  • Rat hepatic microsomal glucose-6-phosphatase protein levels are increased in streptozotocin-induced diabetes.

    abstract::Hepatic microsomal glucose-6-phosphatase activity levels and the hepatic output of glucose are increased in diabetes. We have used protein chemistry and immunological techniques to determine the mechanism by which the activity levels of the glucose-6-phosphatase system are increased in streptozotocin-induced diabetic ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00291077

    authors: Burchell A,Cain DI

    更新日期:1985-11-01 00:00:00

  • SDF-1-CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1-SLIT2 interactions in mice.

    abstract:AIMS/HYPOTHESIS:We had previously reported that stromal cell-derived factor 1 (SDF-1) mediates chemorepulsion of diabetogenic T cell adhesion to islet microvascular endothelium through unknown mechanisms in NOD mice. Here we report that SDF-1-mediated chemorepulsion occurs through slit homologue (SLIT)2-roundabout, axo...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-013-2978-x

    authors: Glawe JD,Mijalis EM,Davis WC,Barlow SC,Gungor N,McVie R,Kevil CG

    更新日期:2013-10-01 00:00:00

  • Resistance to tolerance induction in the diabetes-prone biobreeding rat as one manifestation of abnormal responses to superantigens.

    abstract::T cells taken from normal rats treated with an exogenous source of bacterial superantigen in vivo specifically failed to proliferate following re-stimulation with the same superantigen in vitro. Responsiveness was restored following the addition of an exogenous source of interleukin-2 indicating that the T cells had b...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00400410

    authors: Sellins KS,Gold DP,Bellgrau D

    更新日期:1996-01-01 00:00:00

  • Potassium intake and risk of incident type 2 diabetes mellitus: the Coronary Artery Risk Development in Young Adults (CARDIA) Study.

    abstract:AIMS/HYPOTHESIS:Serum potassium has been found to be a significant predictor of diabetes risk, but the effect of dietary potassium on diabetes risk is not clear. We sought to determine if dietary potassium is associated with risk of incident type 2 diabetes in young adults. METHODS:We used data from the Coronary Arter...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-012-2487-3

    authors: Chatterjee R,Colangelo LA,Yeh HC,Anderson CA,Daviglus ML,Liu K,Brancati FL

    更新日期:2012-05-01 00:00:00

  • The UK case-control study of cerebral oedema complicating diabetic ketoacidosis in children.

    abstract:AIMS/HYPOTHESIS:Cerebral oedema complicating diabetic ketoacidosis (DKA) remains the major cause of morbidity and mortality in children with type 1 diabetes, but its aetiology remains unknown. Our objective was to determine the impact of baseline biochemical factors and of treatment-related variables on risk of the dev...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-006-0363-8

    authors: Edge JA,Jakes RW,Roy Y,Hawkins M,Winter D,Ford-Adams ME,Murphy NP,Bergomi A,Widmer B,Dunger DB

    更新日期:2006-09-01 00:00:00

  • The effect of insulin and exercise on c-Cbl protein abundance and phosphorylation in insulin-resistant skeletal muscle in lean and obese Zucker rats.

    abstract:AIMS/HYPOTHESIS:Recruitment of the protein c-Cbl to the insulin receptor (IR) and its tyrosine phosphorylation via a pathway that is independent from phosphatidylinositol 3'-kinase is necessary for insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes. The activation of this pathway by insulin or exercise has yet...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-003-1322-2

    authors: Wadley GD,Bruce CR,Konstantopoulos N,Macaulay SL,Howlett KF,Hawley JA,Cameron-Smith D

    更新日期:2004-03-01 00:00:00

  • Symptoms, signs and complications in newly diagnosed type 2 diabetic patients, and their relationship to glycaemia, blood pressure and weight.

    abstract:AIMS/HYPOTHESIS:To document the prevalence of typical diabetic symptoms, signs and complications in the diagnosis of type 2 diabetes mellitus, examine their pre-diagnostic duration, and analyse associations with glycaemic level, blood pressure (BP), and weight. METHODS:An epidemiological population-based study of 1137...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-004-1625-y

    authors: Drivsholm T,de Fine Olivarius N,Nielsen AB,Siersma V

    更新日期:2005-02-01 00:00:00

  • Estimation of individual beneficial and adverse effects of intensive glucose control for patients with type 2 diabetes.

    abstract:AIMS/HYPOTHESIS:Intensive glucose control reduces the risk of vascular complications while increasing the risk of severe hypoglycaemia at a group level. We sought to estimate individual beneficial and adverse effects of intensive glucose control in patients with type 2 diabetes. METHODS:We performed a post hoc analysi...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-016-4082-5

    authors: van der Leeuw J,Visseren FL,Woodward M,van der Graaf Y,Grobbee DE,Harrap S,Heller S,Mancia G,Marre M,Poulter N,Zoungas S,Chalmers J

    更新日期:2016-12-01 00:00:00

  • Hyperinsulinaemia and hyperglycaemia: possible risk factors of colorectal cancer among diabetic patients.

    abstract::Hyperinsulinaemia and hyperglycaemia are two possible risk factors for colorectal cancer, which constitutes the third leading cause of cancer death in Western countries. Molecular evidence as well as animal models provide support for these associations: Insulin has been shown to be an important growth factor for colon...

    journal_title:Diabetologia

    pub_type: 杂志文章,评审

    doi:10.1007/s00125-003-1109-5

    authors: Chang CK,Ulrich CM

    更新日期:2003-05-01 00:00:00

  • Conditional islet hypovascularisation does not preclude beta cell expansion during pregnancy in mice.

    abstract:AIMS/HYPOTHESIS:Endothelial-endocrine cell interactions and vascular endothelial growth factor (VEGF)-A signalling are deemed essential for maternal islet vascularisation, glucose control and beta cell expansion during mouse pregnancy. The aim of this study was to assess whether pregnancy-associated beta cell expansion...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-017-4243-1

    authors: Staels W,Heremans Y,Leuckx G,Van Gassen N,Salinno C,De Groef S,Cools M,Keshet E,Dor Y,Heimberg H,De Leu N

    更新日期:2017-06-01 00:00:00

  • Treatment of diabetes mellitus.

    abstract::New treatment strategies for subjects with Type 1 (insulin-dependent) and Type 2 (non-insulin-dependent) diabetes mellitus are being developed. Pilot studies utilising insulin itself have been reported to prevent Type 1 diabetes in subjects likely, by immunogenetic and physiologic criteria, to develop clinically overt...

    journal_title:Diabetologia

    pub_type: 杂志文章,评审

    doi:10.1007/BF00586274

    authors: Robertson RP,Klein DJ

    更新日期:1992-12-01 00:00:00

  • Reclassification of asymptomatic beta cell autoimmunity: a critical perspective.

    abstract::Type 1 diabetes is an immune-mediated disease leading to almost total beta cell destruction and permanent exogenous insulin dependency. The appearance of clinical symptoms is preceded by an asymptomatic preclinical period, the duration of which is highly individual. The emergence of diabetes-associated autoantibodies ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-016-4147-5

    authors: Knip M,Selvenius J,Siljander H,Veijola R

    更新日期:2017-01-01 00:00:00

  • The influence of the ACE ( I/D) polymorphism on systemic and renal vascular responses to angiotensins in normotensive, normoalbuminuric Type 1 diabetes mellitus.

    abstract:AIM/HYPOTHESIS:The renin-angiotensin-aldosterone system is important in diabetic nephropathy, with the angiotensin-converting-enzyme DD-genotype being a renal risk factor. The D-allele is associated with higher ACE concentrations, but functional consequences in diabetes mellitus are not known. To analyse these conseque...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-003-1149-x

    authors: Luik PT,Hoogenberg K,Kerstens MN,Beusekamp BJ,De Jong PE,Dullaart RP,Navis GJ

    更新日期:2003-08-01 00:00:00

  • Acute and chronic effects of troglitazone (CS-045) on isolated rat ventricular cardiomyocytes.

    abstract::Freshly isolated and primary cultured adult rat cardiomyocytes were used to elucidate the mechanism of action of the new oral antidiabetic agent (+/-)-5-[4-(6-hydroxy-2, 5, 7, 8-tetramethyl-chroman-2-yl-methoxy)benzyl]-2,4-thiazolidinedione (troglitazone) on the heart. Interaction with protein kinase C (PKC) and regul...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s001250050509

    authors: Bähr M,Spelleken M,Bock M,von Holtey M,Kiehn R,Eckel J

    更新日期:1996-07-01 00:00:00

  • Inhibition of development of peripheral neuropathy in streptozotocin-induced diabetic rats with N-acetylcysteine.

    abstract::N-acetylcysteine (NAC) is a precursor of glutathione (GSH) synthesis, a free radical scavenger and an inhibitor of tumour necrosis factor alpha (TNF). Because these functions might be beneficial in diabetic complications, in this study we examined whether NAC inhibits peripheral neuropathy. Motor nerve conduction velo...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00418340

    authors: Sagara M,Satoh J,Wada R,Yagihashi S,Takahashi K,Fukuzawa M,Muto G,Muto Y,Toyota T

    更新日期:1996-03-01 00:00:00

  • MST1 coordinately regulates autophagy and apoptosis in diabetic cardiomyopathy in mice.

    abstract:AIMS/HYPOTHESIS:Diabetic cardiomyopathy (DCM) is associated with suppressed autophagy and augmented apoptosis in the heart although the interplay between the two remains elusive. The ability of mammalian sterile 20-like kinase 1 to regulate both autophagy and apoptosis prompted us to investigate it as a possible candid...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-016-4070-9

    authors: Zhang M,Zhang L,Hu J,Lin J,Wang T,Duan Y,Man W,Feng J,Sun L,Jia H,Li C,Zhang R,Wang H,Sun D

    更新日期:2016-11-01 00:00:00